Isolated polynucleotides and polypeptides for increasing plant yield and/or agricultural characteristics

ABSTRACT

Provided are isolated polynucleotides which comprise a nucleic acid sequence at least 80% identical to SEQ ID NO: 321, 1-320, 322-480, 793-2945 or 2946; isolated polypeptides which comprise an amino acid sequence at least 80% homologous to SEQ ID NO: 517, 481-516, 518-792, 2947-4662 or 4663, nucleic acid constructs comprising same, transgenic cells and plants expressing same and methods of using same for increasing yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality, abiotic stress tolerance, and/or nitrogen use efficiency of a plant.

RELATED APPLICATIONS

This application is a division of U.S. patent application Ser. No.13/695,094 filed on Oct. 29, 2012, which is a National Phase of PCTPatent Application No. PCT/M2011/051843 having International filing dateof Apr. 27, 2011, which claims the benefit of priority under 35 USC§119(e) of U.S. Provisional Patent Application Nos. 61/328,692 filed onApr. 28, 2010, 61/378,003 filed on Aug. 30, 2010, 61/405,260 filed onOct. 21, 2010 and 61/437,715 filed on Jan. 31, 2011. The contents of theabove applications are all incorporated by reference as if fully setforth herein in their entirety.

SEQUENCE LISTING STATEMENT

The ASCII file, entitled 65571SequenceListing.txt, created on Mar. 15,2016, comprising 12,197,419 bytes, submitted concurrently with thefiling of this application is incorporated herein by reference.

FIELD AND BACKGROUND OF THE INVENTION

The present invention, in some embodiments thereof, relates to isolatedpolynucleotides and polypeptides which can increase the yield (e.g.,biomass, grain quantity and/or quality), growth rate, vigor, abioticstress tolerance (ABST), water use efficiency (WUE), nitrogen useefficiency (NUE) and/or fertilizer use efficiency (FUE) of a plant.

The ever-increasing world population and the decreasing availability inarable land for agriculture affect the yield of plants and plant-relatedproducts. The global shortage of water supply, desertification, abioticstress (ABS) conditions (e.g., salinity, drought, flood, suboptimaltemperature and toxic chemical pollution), and/or limited nitrogen andfertilizer sources cause substantial damage to agricultural plants suchas major alterations in the plant metabolism, cell death, and decreasesin plant growth and crop productivity.

Drought is a gradual phenomenon, which involves periods of abnormallydry weather that persists long enough to produce serious hydrologicimbalances such as crop damage, water supply shortage and increasedsusceptibility to various diseases.

Salinity, high salt levels, affects one in five hectares of irrigatedland. None of the top five food crops, i.e., wheat, corn, rice,potatoes, and soybean, can tolerate excessive salt. Detrimental effectsof salt on plants result from both water deficit, which leads to osmoticstress (similar to drought stress), and the effect of excess sodium ionson critical biochemical processes. As with freezing and drought, highsalt causes water deficit; and the presence of high salt makes itdifficult for plant roots to extract water from their environment. Thus,salination of soils that are used for agricultural production is asignificant and increasing problem in regions that rely heavily onagriculture, and is worsen by over-utilization, over-fertilization andwater shortage, typically caused by climatic change and the demands ofincreasing population.

Suboptimal temperatures affect plant growth and development through thewhole plant life cycle. Thus, low temperatures reduce germination rateand high temperatures result in leaf necrosis. In addition, matureplants that are exposed to excess heat may experience heat shock, whichmay arise in various organs, including leaves and particularly fruit,when transpiration is insufficient to overcome heat stress. Heat alsodamages cellular structures, including organelles and cytoskeleton, andimpairs membrane function. Heat shock may produce a decrease in overallprotein synthesis, accompanied by expression of heat shock proteins,e.g., chaperones, which are involved in refolding proteins denatured byheat. High-temperature damage to pollen almost always occurs inconjunction with drought stress, and rarely occurs under well-wateredconditions. Combined stress can alter plant metabolism in novel ways.Excessive chilling conditions, e.g., low, but above freezing,temperatures affect crops of tropical origins, such as soybean, rice,maize, and cotton. Typical chilling damage includes wilting, necrosis,chlorosis or leakage of ions from cell membranes. Excessive lightconditions, which occur under clear atmospheric conditions subsequent tocold late summer/autumn nights, can lead to photoinhibition ofphotosynthesis (disruption of photosynthesis). In addition, chilling maylead to yield losses and lower product quality through the delayedripening of maize.

Nutrient deficiencies cause adaptations of the root architecture,particularly notably for example is the root proliferation withinnutrient rich patches to increase nutrient uptake. Nutrient deficienciescause also the activation of plant metabolic pathways which maximize theabsorption, assimilation and distribution processes such as byactivating architectural changes. Engineering the expression of thetriggered genes may cause the plant to exhibit the architectural changesand enhanced metabolism also under other conditions.

In addition, it is widely known that the plants usually respond to waterdeficiency by creating a deeper root system that allows access tomoisture located in deeper soil layers. Triggering this effect willallow the plants to access nutrients and water located in deeper soilhorizons particularly those readily dissolved in water like nitrates.

Suboptimal nutrient (macro and micro nutrient) affect plant growth anddevelopment through the whole plant life cycle. A common approach topromote plant growth has been, and continues to be, the use of naturalas well as synthetic nutrients (fertilizers). Thus, fertilizers are thefuel behind the “green revolution”, directly responsible for theexceptional increase in crop yields during the last 40 years, and areconsidered the number one overhead expense in agriculture. Of the threemacronutrients provided as main fertilizers [Nitrogen (N), Phosphate (P)and Potassium (K)], nitrogen is often the rate-limiting element in plantgrowth and all field crops have a fundamental dependence on inorganicnitrogenous fertilizer. Nitrogen is responsible for biosynthesis ofamino acids and nucleic acids, prosthetic groups, plant hormones, plantchemical defenses, and the like; it is translocated to the shoot, whereit is stored in the leaves and stalk during the rapid step of plantdevelopment and up until flowering. In corn for example, plantsaccumulate the bulk of their organic nitrogen during the period of graingermination, and until flowering. Once fertilization of the plant hasoccurred, grains begin to form and become the main sink of plantnitrogen. The stored nitrogen can be then redistributed from the leavesand stalk that served as storage compartments until grain formation.Phosphorous (P) and Potassium (K) have a direct correlation to yield andgeneral plant tolerance.

Since fertilizer is rapidly depleted from most soil types, it must besupplied to growing crops two or three times during the growing season,particularly for cereals, which comprise more than half of thecultivated areas worldwide. For example, inorganic nitrogenousfertilizers such as ammonium nitrate, potassium nitrate, or urea,typically accounts for 40% of the costs associated with crops such ascorn and wheat. In addition, the low nitrogen use efficiency (NUE) ofthe main crops (e.g., in the range of only 30-70%) negatively affectsthe input expenses for the farmer, due to the excess fertilizer applied.Moreover, the over and inefficient use of fertilizers are major factorsresponsible for environmental problems such as eutrophication ofgroundwater, lakes, rivers and seas, nitrate pollution in drinking waterwhich can cause methemoglobinemia, phosphate pollution, atmosphericpollution and the like. However, in spite of the negative impact offertilizers on the environment, and the limits on fertilizer use, whichhave been legislated in several countries, the use of fertilizers isexpected to increase in order support food and fiber production forrapid population growth on limited land resources. For example, it hasbeen estimated that by 2050, more than 150 million tons of nitrogenousfertilizer will be used worldwide annually. Increased use efficiency ofnitrogen by plants should enable crops to be cultivated with lowerfertilizer input, or alternatively to be cultivated on soils of poorerquality and would therefore have significant economic impact in bothdeveloped and developing agricultural systems.

Yield is affected by various factors, such as, the number and size ofthe plant organs, plant architecture (for example, the number ofbranches), grains set length, number of filled grains, vigor (e.g.seedling), growth rate, root development, utilization of water,nutrients (e.g., nitrogen) and fertilizers, and stress tolerance.

Crops such as, corn, rice, wheat, canola and soybean account for overhalf of total human caloric intake, whether through direct consumptionof the seeds themselves or through consumption of meat products raisedon processed seeds or forage. Seeds are also a source of sugars, oilsand metabolites used in industrial processes. The ability to increaseplant yield, whether through increase dry matter accumulation rate,modifying cellulose or lignin composition, increase stalk strength,enlarge meristem size, change of plant branching pattern, erectness oflevees, increase in fertilization efficiency, enhanced seed dry matteraccumulation rate, modification of seed development, enhanced seedfilling or by increasing the content of oil, starch or protein in theseeds would have many applications in agricultural and non-agriculturaluses such as in the biotechnological production of pharmaceuticals,antibodies or vaccines.

Studies have shown that plant adaptations to adverse environmentalconditions are complex genetic traits with polygenic nature.Conventional means for crop and horticultural improvements utilizeselective breeding techniques to identify plants having desirablecharacteristics. However, selective breeding is tedious, time consumingand has an unpredictable outcome. Furthermore, limited germplasmresources for yield improvement and incompatibility in crosses betweendistantly related plant species represent significant problemsencountered in conventional breeding. Advances in genetic engineeringhave allowed mankind to modify the germplasm of plants by expression ofgenes-of-interest in plants. Such a technology has the capacity togenerate crops or plants with improved economic, agronomic orhorticultural traits.

Genetic improvement of fertilizer use efficiency (FUE) in plants can begenerated either via traditional breeding or via genetic engineering.Attempts to generate plants with increased FUE have been described inU.S. Pat. Appl. No. 20020046419 to Choo, et al.; U.S. Pat. Appl. No.2005010879 to Edgerton et al.; U.S. Pat. Appl. No. 20060179511 to Chometet al.; Good, A, et al. 2007 (Engineering nitrogen use efficiency withalanine aminotransferase. Canadian Journal of Botany 85: 252-262); andGood A G et al. 2004 (Trends Plant Sci. 9:597-605).

Yanagisawa et al. (Proc. Natl. Acad. Sci. U.S. Pat. No.2,004,101:7833-8) describe Dofl transgenic plants which exhibit improvedgrowth under low-nitrogen conditions.

U.S. Pat. No. 6,084,153 to Good et al. discloses the use of a stressresponsive promoter to control the expression of Alanine AmineTransferase (AlaAT) and transgenic canola plants with improved droughtand nitrogen deficiency tolerance when compared to control plants.

WO publication No. 2009/013750 discloses genes, constructs and methodsof increasing abiotic stress tolerance, biomass and/or yield in plantsgenerated thereby.

WO publication No. 2008/122980 discloses genes constructs and methodsfor increasing oil content, growth rate and biomass of plants.

WO publication No. 2008/075364 discloses polynucleotides involved inplant fiber development and methods of using same.

WO publication No. 2007/049275 discloses isolated polypeptides,polynucleotides encoding same, transgenic plants expressing same andmethods of using same for increasing plant abiotic stress tolerance andbiomass.

WO publication No. 2004/104162 discloses methods of increasing abioticstress tolerance and/or biomass in plants and plants generated thereby.

WO publication No. 2005/121364 discloses polynucleotides andpolypeptides involved in plant fiber development and methods of usingsame for improving fiber quality, yield and/or biomass of a fiberproducing plant.

WO publication No. 2007/020638 discloses methods of increasing abioticstress tolerance and/or biomass in plants and plants generated thereby.

WO publication No. 2009/083958 discloses methods of increasing water useefficiency, fertilizer use efficiency, biotic/abiotic stress tolerance,yield and biomass in plant and plants generated thereby.

WO publication No. 2010/020941 discloses methods of increasing nitrogenuse efficiency, abiotic stress tolerance, yield and biomass in plantsand plants generated thereby.

WO publication No. 2009/141824 discloses isolated polynucleotides andmethods using same for increasing plant utility.

SUMMARY OF THE INVENTION

According to an aspect of some embodiments of the present inventionthere is provided a method of increasing yield, biomass, growth rate,vigor, oil content, fiber yield, fiber quality, abiotic stresstolerance, and/or nitrogen use efficiency of a plant, comprisingexpressing within the plant an exogenous polynucleotide comprising anucleic acid sequence encoding a polypeptide at least 80% identical toSEQ ID NO: 481-792, 2947-4662 or 4663, thereby increasing the yield,biomass, growth rate, vigor, oil content, fiber yield, fiber quality,abiotic stress tolerance, and/or nitrogen use efficiency of the plant.

According to an aspect of some embodiments of the present inventionthere is provided a method of increasing yield, biomass, growth rate,vigor, oil content, fiber yield, fiber quality, abiotic stresstolerance, and/or nitrogen use efficiency of a plant, comprisingexpressing within the plant an exogenous polynucleotide comprising anucleic acid sequence encoding a polypeptide selected from the groupconsisting of SEQ ID NOs:481-792, and 2947-4663, thereby increasing theyield, biomass, growth rate, vigor, oil content, fiber yield, fiberquality, abiotic stress tolerance, and/or nitrogen use efficiency of theplant.

According to an aspect of some embodiments of the present inventionthere is provided a method of increasing yield, biomass, growth rate,vigor, oil content, fiber yield, fiber quality, abiotic stresstolerance, and/or nitrogen use efficiency of a plant, comprisingexpressing within the plant an exogenous polynucleotide comprising anucleic acid sequence at least 80% identical to SEQ ID NO: 1-480,793-2945, or 2946, thereby increasing the yield, biomass, growth rate,vigor, oil content, fiber yield, fiber quality, abiotic stresstolerance, and/or nitrogen use efficiency of the plant.

According to an aspect of some embodiments of the present inventionthere is provided a method of increasing yield, biomass, growth rate,vigor, oil content, fiber yield, fiber quality, abiotic stresstolerance, and/or nitrogen use efficiency of a plant, comprisingexpressing within the plant an exogenous polynucleotide comprising thenucleic acid sequence selected from the group consisting of SEQ ID NOs:1-480, and 793-2946, thereby increasing the yield, biomass, growth rate,vigor, oil content, fiber yield, fiber quality, abiotic stresstolerance, and/or nitrogen use efficiency of the plant.

According to an aspect of some embodiments of the present inventionthere is provided an isolated polynucleotide comprising a nucleic acidsequence encoding a polypeptide which comprises an amino acid sequenceat least 80% homologous to the amino acid sequence set forth in SEQ IDNO:481-792, 2947-4662 or 4663, wherein the amino acid sequence iscapable of increasing yield, biomass, growth rate, vigor, oil content,fiber yield, fiber quality, abiotic stress tolerance, and/or nitrogenuse efficiency of a plant.

According to an aspect of some embodiments of the present inventionthere is provided an isolated polynucleotide comprising a nucleic acidsequence encoding a polypeptide which comprises the amino acid sequenceselected from the group consisting of SEQ ID NOs:481-792, and 2947-4663.

According to an aspect of some embodiments of the present inventionthere is provided an isolated polynucleotide comprising a nucleic acidsequence at least 80% identical to SEQ ID NO:1-480, 793-2945 or 2946,wherein the nucleic acid sequence is capable of increasing yield,biomass, growth rate, vigor, oil content, fiber yield, fiber quality,abiotic stress tolerance, and/or nitrogen use efficiency of a plant.

According to an aspect of some embodiments of the present inventionthere is provided an isolated polynucleotide comprising the nucleic acidsequence selected from the group consisting of SEQ ID NOs: 1-480, and793-2946.

According to an aspect of some embodiments of the present inventionthere is provided a nucleic acid construct comprising the isolatedpolynucleotide of some embodiments of the invention, and a promoter fordirecting transcription of the nucleic acid sequence in a host cell.

According to an aspect of some embodiments of the present inventionthere is provided an isolated polypeptide comprising an amino acidsequence at least 80% homologous to SEQ ID NO: 481-792, 2947-4662 or4663, wherein the amino acid sequence is capable of increasing yield,biomass, growth rate, vigor, oil content, fiber yield, fiber quality,abiotic stress tolerance, and/or nitrogen use efficiency of a plant.

According to an aspect of some embodiments of the present inventionthere is provided an isolated polypeptide comprising the amino acidsequence selected from the group consisting of SEQ ID NOs: 481-792, and2947-4663.

According to an aspect of some embodiments of the present inventionthere is provided a plant cell exogenously expressing the polynucleotideof some embodiments of the invention, or the nucleic acid construct ofsome embodiments of the invention.

According to an aspect of some embodiments of the present inventionthere is provided a plant cell exogenously expressing the polypeptide ofsome embodiments of the invention.

According to an aspect of some embodiments of the present inventionthere is provided a transgenic plant comprising the nucleic acidconstruct of some embodiments of the invention.

According to some embodiments of the invention, the nucleic acidsequence encodes an amino acid sequence selected from the groupconsisting of SEQ ID NOs: 481-792, and 2947-4663.

According to some embodiments of the invention, the nucleic acidsequence is selected from the group consisting of SEQ ID NOs: 1-480, and793-2946.

According to some embodiments of the invention, the polynucleotideconsists of the nucleic acid sequence selected from the group consistingof SEQ ID NOs: 1-480, and 793-2946.

According to some embodiments of the invention, the nucleic acidsequence encodes the amino acid sequence selected from the groupconsisting of SEQ ID NOs:481-792, and 2947-4663.

According to some embodiments of the invention, the plant cell formspart of a plant.

According to some embodiments of the invention, the method furthercomprising growing the plant expressing the exogenous polynucleotideunder the abiotic stress.

According to some embodiments of the invention, the abiotic stress isselected from the group consisting of salinity, drought, waterdeprivation, flood, etiolation, low temperature, high temperature, heavymetal toxicity, anaerobiosis, nutrient deficiency, nutrient excess,atmospheric pollution and UV irradiation.

According to some embodiments of the invention, the yield comprises seedyield or oil yield.

According to some embodiments of the invention, the method furthercomprising growing the plant expressing the exogenous polynucleotideunder nitrogen-limiting conditions.

According to some embodiments of the invention, the promoter isheterologous to the isolated polynucleotide and/or to the host cell.

Unless otherwise defined, all technical and/or scientific terms usedherein have the same meaning as commonly understood by one of ordinaryskill in the art to which the invention pertains. Although methods andmaterials similar or equivalent to those described herein can be used inthe practice or testing of embodiments of the invention, exemplarymethods and/or materials are described below. In case of conflict, thepatent specification, including definitions, will control. In addition,the materials, methods, and examples are illustrative only and are notintended to be necessarily limiting.

BRIEF DESCRIPTION OF THE SEVERAL VIEWS OF THE DRAWING(S)

Some embodiments of the invention are herein described, by way ofexample only, with reference to the accompanying drawings. With specificreference now to the drawings in detail, it is stressed that theparticulars shown are by way of example and for purposes of illustrativediscussion of embodiments of the invention. In this regard, thedescription taken with the drawings makes apparent to those skilled inthe art how embodiments of the invention may be practiced.

In the drawings:

FIG. 1 is a schematic illustration of the modified pGI binary plasmidcontaining the new At6669 promoter (SEQ ID NO:4668) and the GUSintron(pQYN_6669) used for expressing the isolated polynucleotide sequences ofthe invention. RB—T-DNA right border; LB—T-DNA left border; MCS—Multiplecloning site; RE—any restriction enzyme; NOS pro=nopaline synthasepromoter; NPT-II=neomycin phosphotransferase gene; NOS ter=nopalinesynthase terminator; Poly-A signal (polyadenylation signal);GUSintron—the GUS reporter gene (coding sequence and intron). Theisolated polynucleotide sequences of the invention were cloned into thevector while replacing the GUSintron reporter gene.

FIG. 2 is a schematic illustration of the modified pGI binary plasmidcontaining the new At6669 promoter (SEQ ID NO:4668) (pQFN or pQFNc) usedfor expressing the isolated polynucleotide sequences of the invention.RB—T-DNA right border; LB—T-DNA left border; MCS—Multiple cloning site;RE—any restriction enzyme; NOS pro=nopaline synthase promoter;NPT-II=neomycin phosphotransferase gene; NOS ter=nopaline synthaseterminator; Poly-A signal (polyadenylation signal); GUSintron—the GUSreporter gene (coding sequence and intron). The isolated polynucleotidesequences of the invention were cloned into the MCS of the vector.

FIGS. 3A-3F are images depicting visualization of root development oftransgenic plants exogenously expressing the polynucleotide of someembodiments of the invention when grown in transparent agar plates undernormal (FIGS. 3A-B), osmotic stress (15% PEG; FIGS. 3C-D) ornitrogen-limiting (FIGS. 3E-F) conditions. The different transgenes weregrown in transparent agar plates for 17 days (7 days nursery and 10 daysafter transplanting). The plates were photographed every 3-4 daysstarting at day 1 after transplanting. FIG. 3A—An image of a photographof plants taken following 10 after transplanting days on agar plateswhen grown under normal (standard) conditions. FIG. 3B—An image of rootanalysis of the plants shown in FIG. 3A in which the lengths of theroots measured are represented by arrows. FIG. 3C—An image of aphotograph of plants taken following 10 days after transplanting on agarplates, grown under high osmotic (PEG 15%) conditions. FIG. 3D—An imageof root analysis of the plants shown in FIG. 3C in which the lengths ofthe roots measured are represented by arrows. FIG. 3E—An image of aphotograph of plants taken following 10 days after transplanting on agarplates, grown under low nitrogen conditions. FIG. 3F—An image of rootanalysis of the plants shown in FIG. 3E in which the lengths of theroots measured are represented by arrows.

FIG. 4 is a schematic illustration of the modified pGI binary plasmidcontaining the Root Promoter (pQNa_RP) used for expressing the isolatedpolynucleotide sequences of the invention. RB—T-DNA right border;LB—T-DNA left border; NOS pro=nopaline synthase promoter;NPT-II=neomycin phosphotransferase gene; NOS ter=nopaline synthaseterminator; Poly-A signal (polyadenylation signal); The isolatedpolynucleotide sequences according to some embodiments of the inventionwere cloned into the MCS of the vector.

FIG. 5 is a schematic illustration of the pQYN plasmid.

FIG. 6 is a schematic illustration of the pQFN plasmid.

FIG. 7 is a schematic illustration of the pQFYN plasmid.

FIG. 8 is a schematic illustration of pQXNc plasmid, which is a modifiedpGI binary plasmid used for expressing the isolated polynucleotidesequences of some embodiments of the invention. RB—T-DNA right border;LB—T-DNA left border; NOS pro=nopaline synthase promoter;NPT-II=neomycin phosphotransferase gene; NOS ter=nopaline synthaseterminator; RE=any restriction enzyme; Poly-A signal (polyadenylationsignal); 35S—the 35S promoter (SEQ ID NO:4666). The isolatedpolynucleotide sequences of some embodiments of the invention werecloned into the MCS (Multiple cloning site) of the vector.

DESCRIPTION OF SPECIFIC EMBODIMENTS OF THE INVENTION

The present invention, in some embodiments thereof, relates to isolatedpolynucleotides and polypeptides, nucleic acid constructs, transgeniccells and transgenic plants comprising same and methods of generatingand using same, and, more particularly, but not exclusively, to methodsof increasing yield, biomass, growth rate, vigor, oil content, fiberyield, fiber quality abiotic stress tolerance, and/or fertilizer useefficiency (e.g., nitrogen use efficiency) of a plant.

Before explaining at least one embodiment of the invention in detail, itis to be understood that the invention is not necessarily limited in itsapplication to the details set forth in the following description orexemplified by the Examples. The invention is capable of otherembodiments or of being practiced or carried out in various ways.

The present inventors have identified novel polypeptides andpolynucleotides which can be used to increase yield, growth rate,biomass, oil content, vigor and/or abiotic stress tolerance of a plant.

Thus, as shown in the Examples section which follows, the presentinventors have utilized bioinformatics tools to identify polynucleotideswhich enhance yield (e.g., seed yield, oil yield, oil content), growthrate, biomass, vigor, abiotic stress tolerance and/or fertilizer (e.g.,nitrogen) use efficiency of a plant. Genes which affect thetrait-of-interest were identified [Example 1, Table 1, SEQ ID NOs: 1-288(polynucleotides) and 481-727 (polypeptides)] based on expressionprofiles in specific tissues and conditions of several Barley accessions(Example 3, Tables 3-8), Arabidopsis ecotypes/accessions (Examples 4-5,Tables 9-16), Sorghum varieties (Example 6, Tables 17-25) and Maizehybrids (Example 7, Tables 26-31). Homologous polypeptides andpolynucleotides having the same function were also identified [Example2, Table 2, SEQ ID NOs: 793-2946 (polynucleotides) and 2947-4663(polypeptides)]. Agrobacterium tumefaciens cells were transformed withbinary vectors harboring the identified genes (Example 9) and transgenicplants expressing same were generated (Example 10). Transgenic plantsover-expressing the identified polynucleotides were found to exhibitincreased biomass, yield, oil yield, dry matter, harvest index, growthrate, rosette area, seed yield and weight of 1000 seeds (Tables 33-48;Examples 11 and 12). Altogether, these results suggest the use of thenovel polynucleotides and polypeptides of the invention for increasingyield (including oil yield, seed yield and oil content), growth rate,biomass, vigor, abiotic stress tolerance and/or fertilizer (e.g.,nitrogen) use efficiency of a plant.

Thus, according to an aspect of some embodiments of the invention, thereis provided method of increasing yield, biomass, growth rate, vigor, oilcontent, fiber yield, fiber quality, abiotic stress tolerance, and/ornitrogen use efficiency of a plant, comprising expressing within theplant an exogenous polynucleotide comprising a nucleic acid sequenceencoding a polypeptide at least about 80%, at least about 81%, at leastabout 82%, at least about 83%, at least about 84%, at least about 85%,at least about 86%, at least about 87%, at least about 88%, at leastabout 89%, at least about 90%, at least about 91%, at least about 92%,at least about 93%, at least about 94%, at least about 95%, at leastabout 96%, at least about 97%, at least about 98%, at least about 99%,or more say 100% homologous to the amino acid sequence selected from thegroup consisting of SEQ ID NOs: 481-792, and 2947-4663, therebyincreasing the yield, biomass, growth rate, vigor, oil content, fiberyield, fiber quality, abiotic stress tolerance, and/or nitrogen useefficiency of the plant.

As used herein the phrase “plant yield” refers to the amount (e.g., asdetermined by weight or size) or quantity (numbers) of tissues or organsproduced per plant or per growing season. Hence increased yield couldaffect the economic benefit one can obtain from the plant in a certaingrowing area and/or growing time.

It should be noted that a plant yield can be affected by variousparameters including, but not limited to, plant biomass; plant vigor;growth rate; seed yield; seed or grain quantity; seed or grain quality;oil yield; content of oil, starch and/or protein in harvested organs(e.g., seeds or vegetative parts of the plant); number of flowers(florets) per panicle (expressed as a ratio of number of filled seedsover number of primary panicles); harvest index; number of plants grownper area; number and size of harvested organs per plant and per area;number of plants per growing area (density); number of harvested organsin field; total leaf area; carbon assimilation and carbon partitioning(the distribution/allocation of carbon within the plant); resistance toshade; number of harvestable organs (e.g. seeds), seeds per pod, weightper seed; and modified architecture [such as increase stalk diameter,thickness or improvement of physical properties (e.g. elasticity)].

As used herein the phrase “seed yield” refers to the number or weight ofthe seeds per plant, seeds per pod, or per growing area or to the weightof a single seed, or to the oil extracted per seed. Hence seed yield canbe affected by seed dimensions (e.g., length, width, perimeter, areaand/or volume), number of (filled) seeds and seed filling rate and byseed oil content. Hence increase seed yield per plant could affect theeconomic benefit one can obtain from the plant in a certain growing areaand/or growing time; and increase seed yield per growing area could beachieved by increasing seed yield per plant, and/or by increasing numberof plants grown on the same given area.

The term “seed” (also referred to as “grain” or “kernel”) as used hereinrefers to a small embryonic plant enclosed in a covering called the seedcoat (usually with some stored food), the product of the ripened ovuleof gymnosperm and angiosperm plants which occurs after fertilization andsome growth within the mother plant.

The phrase “oil content” as used herein refers to the amount of lipidsin a given plant organ, either the seeds (seed oil content) or thevegetative portion of the plant (vegetative oil content) and istypically expressed as percentage of dry weight (10% humidity of seeds)or wet weight (for vegetative portion).

It should be noted that oil content is affected by intrinsic oilproduction of a tissue (e.g., seed, vegetative portion), as well as themass or size of the oil-producing tissue per plant or per growth period.

In one embodiment, increase in oil content of the plant can be achievedby increasing the size/mass of a plant's tissue(s) which comprise oilper growth period. Thus, increased oil content of a plant can beachieved by increasing the yield, growth rate, biomass and vigor of theplant.

As used herein the phrase “plant biomass” refers to the amount (e.g.,measured in grams of air-dry tissue) of a tissue produced from the plantin a growing season, which could also determine or affect the plantyield or the yield per growing area. An increase in plant biomass can bein the whole plant or in parts thereof such as aboveground (harvestable)parts, vegetative biomass, roots and seeds.

As used herein the phrase “growth rate” refers to the increase in plantorgan/tissue size per time (can be measured in cm² per day).

As used herein the phrase “plant vigor” refers to the amount (measuredby weight) of tissue produced by the plant in a given time. Henceincreased vigor could determine or affect the plant yield or the yieldper growing time or growing area. In addition, early vigor (seed and/orseedling) results in improved field stand.

Improving early vigor is an important objective of modern rice breedingprograms in both temperate and tropical rice cultivars. Long roots areimportant for proper soil anchorage in water-seeded rice. Where rice issown directly into flooded fields, and where plants must emerge rapidlythrough water, longer shoots are associated with vigour. Wheredrill-seeding is practiced, longer mesocotyls and coleoptiles areimportant for good seedling emergence. The ability to engineer earlyvigor into plants would be of great importance in agriculture. Forexample, poor early vigor has been a limitation to the introduction ofmaize (Zea mays L.) hybrids based on Corn Belt germplasm in the EuropeanAtlantic.

It should be noted that a plant yield can be determined under stress(e.g., abiotic stress, nitrogen-limiting conditions) and/or non-stress(normal) conditions.

As used herein, the phrase “non-stress conditions” refers to the growthconditions (e.g., water, temperature, light-dark cycles, humidity, saltconcentration, fertilizer concentration in soil, nutrient supply such asnitrogen, phosphorous and/or potassium), that do not significantly gobeyond the everyday climatic and other abiotic conditions that plantsmay encounter, and which allow optimal growth, metabolism, reproductionand/or viability of a plant at any stage in its life cycle (e.g., in acrop plant from seed to a mature plant and back to seed again). Personsskilled in the art are aware of normal soil conditions and climaticconditions for a given plant in a given geographic location. It shouldbe noted that while the non-stress conditions may include some mildvariations from the optimal conditions (which vary from one type/speciesof a plant to another), such variations do not cause the plant to ceasegrowing without the capacity to resume growth.

The phrase “abiotic stress” as used herein refers to any adverse effecton metabolism, growth, reproduction and/or viability of a plant.Accordingly, abiotic stress can be induced by suboptimal environmentalgrowth conditions such as, for example, salinity, water deprivation,flooding, freezing, low or high temperature, heavy metal toxicity,anaerobiosis, nutrient deficiency, atmospheric pollution or UVirradiation. The implications of abiotic stress are discussed in theBackground section.

The phrase “abiotic stress tolerance” as used herein refers to theability of a plant to endure an abiotic stress without suffering asubstantial alteration in metabolism, growth, productivity and/orviability.

Plants are subject to a range of environmental challenges. Several ofthese, including salt stress, general osmotic stress, drought stress andfreezing stress, have the ability to impact whole plant and cellularwater availability. Not surprisingly, then, plant responses to thiscollection of stresses are related. Zhu (2002) Ann. Rev. Plant Biol. 53:247-273 et al. note that “most studies on water stress signaling havefocused on salt stress primarily because plant responses to salt anddrought are closely related and the mechanisms overlap”. Many examplesof similar responses and pathways to this set of stresses have beendocumented. For example, the CBF transcription factors have been shownto condition resistance to salt, freezing and drought (Kasuga et al.(1999) Nature Biotech. 17: 287-291). The Arabidopsis rd29B gene isinduced in response to both salt and dehydration stress, a process thatis mediated largely through an ABA signal transduction process (Uno etal. (2000) Proc. Natl. Acad. Sci. USA 97: 11632-11637), resulting inaltered activity of transcription factors that bind to an upstreamelement within the rd29B promoter. In Mesembryanthemum crystallinum (iceplant), Patharker and Cushman have shown that a calcium-dependentprotein kinase (McCDPK1) is induced by exposure to both drought and saltstresses (Patharker and Cushman (2000) Plant J. 24: 679-691). Thestress-induced kinase was also shown to phosphorylate a transcriptionfactor, presumably altering its activity, although transcript levels ofthe target transcription factor are not altered in response to salt ordrought stress. Similarly, Saijo et al. demonstrated that a ricesalt/drought-induced calmodulin-dependent protein kinase (OsCDPK7)conferred increased salt and drought tolerance to rice whenoverexpressed (Saijo et al. (2000) Plant J. 23: 319-327).

Exposure to dehydration invokes similar survival strategies in plants asdoes freezing stress (see, for example, Yelenosky (1989) Plant Physiol89: 444-451) and drought stress induces freezing tolerance (see, forexample, Siminovitch et al. (1982) Plant Physiol 69: 250-255; and Guy etal. (1992) Planta 188: 265-270). In addition to the induction ofcold-acclimation proteins, strategies that allow plants to survive inlow water conditions may include, for example, reduced surface area, orsurface oil or wax production. In another example increased solutecontent of the plant prevents evaporation and water loss due to heat,drought, salinity, osmoticum, and the like therefore providing a betterplant tolerance to the above stresses.

It will be appreciated that some pathways involved in resistance to onestress (as described above), will also be involved in resistance toother stresses, regulated by the same or homologous genes. Of course,the overall resistance pathways are related, not identical, andtherefore not all genes controlling resistance to one stress willcontrol resistance to the other stresses. Nonetheless, if a geneconditions resistance to one of these stresses, it would be apparent toone skilled in the art to test for resistance to these related stresses.Methods of assessing stress resistance are further provided in theExamples section which follows.

As used herein the phrase “water use efficiency (WUE)” refers to thelevel of organic matter produced per unit of water consumed by theplant, i.e., the dry weight of a plant in relation to the plant's wateruse, e.g., the biomass produced per unit transpiration.

As used herein the phrase “fertilizer use efficiency” refers to themetabolic process(es) which lead to an increase in the plant's yield,biomass, vigor, and growth rate per fertilizer unit applied. Themetabolic process can be the uptake, spread, absorbent, accumulation,relocation (within the plant) and use of one or more of the minerals andorganic moieties absorbed by the plant, such as nitrogen, phosphatesand/or potassium.

As used herein the phrase “fertilizer-limiting conditions” refers togrowth conditions which include a level (e.g., concentration) of afertilizer applied which is below the level needed for normal plantmetabolism, growth, reproduction and/or viability.

As used herein the phrase “nitrogen use efficiency (NUE)” refers to themetabolic process(es) which lead to an increase in the plant's yield,biomass, vigor, and growth rate per nitrogen unit applied. The metabolicprocess can be the uptake, spread, absorbent, accumulation, relocation(within the plant) and use of nitrogen absorbed by the plant.

As used herein the phrase “nitrogen-limiting conditions” refers togrowth conditions which include a level (e.g., concentration) ofnitrogen (e.g., ammonium or nitrate) applied which is below the levelneeded for normal plant metabolism, growth, reproduction and/orviability.

Improved plant NUE and FUE is translated in the field into eitherharvesting similar quantities of yield, while implementing lessfertilizers, or increased yields gained by implementing the same levelsof fertilizers. Thus, improved NUE or FUE has a direct effect on plantyield in the field. Thus, the polynucleotides and polypeptides of someembodiments of the invention positively affect plant yield, seed yield,and plant biomass. In addition, the benefit of improved plant NUE willcertainly improve crop quality and biochemical constituents of the seedsuch as protein yield and oil yield.

It should be noted that improved ABST will confer plants with improvedvigor also under non-stress conditions, resulting in crops havingimproved biomass and/or yield e.g., elongated fibers for the cottonindustry, higher oil content.

The term “fiber” is usually inclusive of thick-walled conducting cellssuch as vessels and tracheids and to fibrillar aggregates of manyindividual fiber cells. Hence, the term “fiber” refers to (a)thick-walled conducting and non-conducting cells of the xylem; (b)fibers of extraxylary origin, including those from phloem, bark, groundtissue, and epidermis; and (c) fibers from stems, leaves, roots, seeds,and flowers or inflorescences (such as those of Sorghum vulgare used inthe manufacture of brushes and brooms).

Example of fiber producing plants, include, but are not limited to,agricultural crops such as cotton, silk cotton tree (Kapok, Ceibapentandra), desert willow, creosote bush, winterfat, balsa, kenaf,roselle, jute, sisal abaca, flax, corn, sugar cane, hemp, ramie, kapok,coir, bamboo, spanish moss and Agave spp. (e.g. sisal).

As used herein the phrase “fiber quality” refers to at least one fiberparameter which is agriculturally desired, or required in the fiberindustry (further described hereinbelow). Examples of such parameters,include but are not limited to, fiber length, fiber strength, fiberfitness, fiber weight per unit length, maturity ratio and uniformity(further described hereinbelow).

Cotton fiber (lint) quality is typically measured according to fiberlength, strength and fineness. Accordingly, the lint quality isconsidered higher when the fiber is longer, stronger and finer.

As used herein the phrase “fiber yield” refers to the amount or quantityof fibers produced from the fiber producing plant.

As used herein the term “increasing” refers to at least about 2%, atleast about 3%, at least about 4%, at least about 5%, at least about10%, at least about 15%, at least about 20%, at least about 30%, atleast about 40%, at least about 50%, at least about 60%, at least about70%, at least about 80%, increase in yield, biomass, growth rate, vigor,oil content, fiber yield, fiber quality, abiotic stress tolerance,and/or nitrogen use efficiency of a plant as compared to a native plant[i.e., a plant not modified with the biomolecules (polynucleotide orpolypeptides) of the invention, e.g., a non-transformed plant of thesame species which is grown under the same (e.g., identical) growthconditions].

The phrase “expressing within the plant an exogenous polynucleotide” asused herein refers to upregulating the expression level of an exogenouspolynucleotide within the plant by introducing the exogenouspolynucleotide into a plant cell or plant and expressing by recombinantmeans, as further described herein below.

As used herein “expressing” refers to expression at the mRNA andoptionally polypeptide level.

As used herein, the phrase “exogenous polynucleotide” refers to aheterologous nucleic acid sequence which may not be naturally expressedwithin the plant or which overexpression in the plant is desired. Theexogenous polynucleotide may be introduced into the plant in a stable ortransient manner, so as to produce a ribonucleic acid (RNA) moleculeand/or a polypeptide molecule. It should be noted that the exogenouspolynucleotide may comprise a nucleic acid sequence which is identicalor partially homologous to an endogenous nucleic acid sequence of theplant.

The term “endogenous” as used herein refers to any polynucleotide orpolypeptide which is present and/or naturally expressed within a plantor a cell thereof. According to some embodiments of the invention, theexogenous polynucleotide of the invention comprises a nucleic acidsequence encoding a polypeptide having an amino acid sequence at leastabout 80%, at least about 81%, at least about 82%, at least about 83%,at least about 84%, at least about 85%, at least about 86%, at leastabout 87%, at least about 88%, at least about 89%, at least about 90%,at least about 91%, at least about 92%, at least about 93%, at leastabout 94%, at least about 95%, at least about 96%, at least about 97%,at least about 98%, at least about 99%, or more say 100% homologous tothe amino acid sequence selected from the group consisting of SEQ IDNOs: 481-792, and 2947-4663.

Homology (e.g., percent homology, identity+similarity) can be determinedusing any homology comparison software, including for example, the BasicLocal Alignment Search Tool BlastP® or Basic Local Alignment Search ToolTBLASTN® software (National Library of Medicine) of the National Centerof Biotechnology Information (NCBI) such as by using default parameters,when starting from a polypeptide sequence; or the Basic Local AlignmentSearch Tool tBLASTX® (National Library of Medicine) algorithm (availablevia the NCBI) such as by using default parameters, which compares thesix-frame conceptual translation products of a nucleotide query sequence(both strands) against a protein sequence database.

According to some embodiments of the invention, the term “homology” or“homologous” refers to identity of two or more nucleic acid sequences;or identity of two or more amino acid sequences.

Homologous sequences include both orthologous and paralogous sequences.The term “paralogous” relates to gene-duplications within the genome ofa species leading to paralogous genes. The term “orthologous” relates tohomologous genes in different organisms due to ancestral relationship.

One option to identify orthologues in monocot plant species is byperforming a reciprocal blast search. This may be done by a first blastinvolving blasting the sequence-of-interest against any sequencedatabase, such as the publicly available NCBI database which may befound at: Hypertext Transfer Protocol://World Wide Web (dot) ncbi (dot)nlm (dot) nih (dot) gov. If orthologues in rice were sought, thesequence-of-interest would be blasted against, for example, the 28,469full-length cDNA clones from Oryza sativa Nipponbare available at NCBI.The blast results may be filtered. The full-length sequences of eitherthe filtered results or the non-filtered results are then blasted back(second blast) against the sequences of the organism from which thesequence-of-interest is derived. The results of the first and secondblasts are then compared. An orthologue is identified when the sequenceresulting in the highest score (best hit) in the first blast identifiesin the second blast the query sequence (the originalsequence-of-interest) as the best hit. Using the same rational aparalogue (homolog to a gene in the same organism) is found. In case oflarge sequence families, the ClustalW program may be used [HypertextTransfer Protocol://World Wide Web (dot) ebi (dot) ac (dot)uk/Tools/clustalw2/index (dot) html], followed by a neighbor-joiningtree (Hypertext Transfer Protocol://en (dot) wikipedia (dot)org/wiki/Neighbor-joining) which helps visualizing the clustering.

According to some embodiments of the invention, the exogenouspolynucleotide of the invention encodes a polypeptide having an aminoacid sequence at least about 80%, at least about 81%, at least about82%, at least about 83%, at least about 84%, at least about 85%, atleast about 86%, at least about 87%, at least about 88%, at least about89%, at least about 90%, at least about 91%, at least about 92%, atleast about 93%, at least about 94%, at least about 95%, at least about96%, at least about 97%, at least about 98%, at least about 99%, or moresay 100% identical to the amino acid sequence selected from the groupconsisting of SEQ ID NOs:481-792, and 2947-4663.

According to some embodiments of the invention, the method of increasingyield, biomass, growth rate, vigor, oil content, fiber yield, fiberquality, abiotic stress tolerance, and/or nitrogen use efficiency of aplant, is effected by expressing within the plant an exogenouspolynucleotide comprising a nucleic acid sequence encoding a polypeptideat least at least about 80%, at least about 81%, at least about 82%, atleast about 83%, at least about 84%, at least about 85%, at least about86%, at least about 87%, at least about 88%, at least about 89%, atleast about 90%, at least about 91%, at least about 92%, at least about93%, at least about 94%, at least about 95%, at least about 96%, atleast about 97%, at least about 98%, at least about 99%, or more say100% identical to the amino acid sequence selected from the groupconsisting of SEQ ID NOs: 481-792, and 2947-4663, thereby increasing theyield, biomass, growth rate, vigor, oil content, fiber yield, fiberquality, abiotic stress tolerance, and/or nitrogen use efficiency of theplant.

According to some embodiments of the invention, the exogenouspolynucleotide encodes a polypeptide consisting of the amino acidsequence set forth by SEQ ID NO:481-792, 2947-4662 or 4663.

According to an aspect of some embodiments of the invention, the methodof increasing yield, biomass, growth rate, vigor, oil content, fiberyield, fiber quality, abiotic stress tolerance, and/or nitrogen useefficiency of a plant, is effected by expressing within the plant anexogenous polynucleotide comprising a nucleic acid sequence encoding apolypeptide comprising an amino acid sequence selected from the groupconsisting of SEQ ID NOs:481-792, and 2947-4663, thereby increasing theyield, biomass, growth rate, vigor, oil content, fiber yield, fiberquality, abiotic stress tolerance, and/or nitrogen use efficiency of theplant.

According to an aspect of some embodiments of the invention, there isprovided a method of increasing yield, biomass, growth rate, vigor, oilcontent, fiber yield, fiber quality, abiotic stress tolerance, and/ornitrogen use efficiency of a plant, comprising expressing within theplant an exogenous polynucleotide comprising a nucleic acid sequenceencoding a polypeptide selected from the group consisting of SEQ ID NOs:481-792, and 2947-4663, thereby increasing the yield, biomass, growthrate, vigor, oil content, fiber yield, fiber quality, abiotic stresstolerance, and/or nitrogen use efficiency of the plant.

According to some embodiments of the invention, the exogenouspolynucleotide encodes a polypeptide consisting of the amino acidsequence set forth by SEQ ID NO: 481-792, 2947-4662 or 4663.

According to some embodiments of the invention the exogenouspolynucleotide comprises a nucleic acid sequence which is at least about80%, at least about 81%, at least about 82%, at least about 83%, atleast about 84%, at least about 85%, at least about 86%, at least about87%, at least about 88%, at least about 89%, at least about 90%, atleast about 91%, at least about 92%, at least about 93%, at least about93%, at least about 94%, at least about 95%, at least about 96%, atleast about 97%, at least about 98%, at least about 99%, e.g., 100%identical to the nucleic acid sequence selected from the groupconsisting of SEQ ID NOs:1-480, and 793-2946.

According to an aspect of some embodiments of the invention, there isprovided a method of increasing yield, biomass, growth rate, vigor, oilcontent, fiber yield, fiber quality, abiotic stress tolerance, and/ornitrogen use efficiency of a plant, comprising expressing within theplant an exogenous polynucleotide comprising a nucleic acid sequence atleast about 80%, at least about 81%, at least about 82%, at least about83%, at least about 84%, at least about 85%, at least about 86%, atleast about 87%, at least about 88%, at least about 89%, at least about90%, at least about 91%, at least about 92%, at least about 93%, atleast about 93%, at least about 94%, at least about 95%, at least about96%, at least about 97%, at least about 98%, at least about 99%, e.g.,100% identical to the nucleic acid sequence selected from the groupconsisting of SEQ ID NOs:1-480, and 793-2946, thereby increasing theyield, biomass, growth rate, vigor, oil content, fiber yield, fiberquality, abiotic stress tolerance, and/or nitrogen use efficiency of theplant.

According to some embodiments of the invention, the homology is a globalhomology, i.e., an homology over the entire amino acid or nucleic acidsequences of the invention and not over portions thereof.

According to some embodiments of the invention, the identity is a globalidentity, i.e., an identity over the entire amino acid or nucleic acidsequences of the invention and not over portions thereof.

Identity (e.g., percent homology) can be determined using any homologycomparison software, including for example, the BlastN software of theNational Center of Biotechnology Information (NCBI) such as by usingdefault parameters.

According to some embodiments of the invention the exogenouspolynucleotide is at least about 80%, at least about 81%, at least about82%, at least about 83%, at least about 84%, at least about 85%, atleast about 86%, at least about 87%, at least about 88%, at least about89%, at least about 90%, at least about 91%, at least about 92%, atleast about 93%, at least about 93%, at least about 94%, at least about95%, at least about 96%, at least about 97%, at least about 98%, atleast about 99%, e.g., 100% identical to the polynucleotide selectedfrom the group consisting of SEQ ID NOs:1-480, and 793-2946.

According to some embodiments of the invention the exogenouspolynucleotide is set forth by SEQ ID NO:1-480, 793-2945 or 2946.

According to some embodiments of the invention the exogenouspolynucleotide is set forth by the nucleic acid sequence selected fromthe group consisting of SEQ ID NOs: 1-480, and 793-2946.

As used herein the term “polynucleotide” refers to a single or doublestranded nucleic acid sequence which is isolated and provided in theform of an RNA sequence, a complementary polynucleotide sequence (cDNA),a genomic polynucleotide sequence and/or a composite polynucleotidesequences (e.g., a combination of the above).

The term “isolated” refers to at least partially separated from thenatural environment e.g., from a plant cell.

As used herein the phrase “complementary polynucleotide sequence” refersto a sequence, which results from reverse transcription of messenger RNAusing a reverse transcriptase or any other RNA dependent DNA polymerase.Such a sequence can be subsequently amplified in vivo or in vitro usinga DNA dependent DNA polymerase.

As used herein the phrase “genomic polynucleotide sequence” refers to asequence derived (isolated) from a chromosome and thus it represents acontiguous portion of a chromosome.

As used herein the phrase “composite polynucleotide sequence” refers toa sequence, which is at least partially complementary and at leastpartially genomic. A composite sequence can include some exonalsequences required to encode the polypeptide of the present invention,as well as some intronic sequences interposing therebetween. Theintronic sequences can be of any source, including of other genes, andtypically will include conserved splicing signal sequences. Suchintronic sequences may further include cis acting expression regulatoryelements.

Nucleic acid sequences encoding the polypeptides of the presentinvention may be optimized for expression. Examples of such sequencemodifications include, but are not limited to, an altered G/C content tomore closely approach that typically found in the plant species ofinterest, and the removal of codons atypically found in the plantspecies commonly referred to as codon optimization.

The phrase “codon optimization” refers to the selection of appropriateDNA nucleotides for use within a structural gene or fragment thereofthat approaches codon usage within the plant of interest. Therefore, anoptimized gene or nucleic acid sequence refers to a gene in which thenucleotide sequence of a native or naturally occurring gene has beenmodified in order to utilize statistically-preferred orstatistically-favored codons within the plant. The nucleotide sequencetypically is examined at the DNA level and the coding region optimizedfor expression in the plant species determined using any suitableprocedure, for example as described in Sardana et al. (1996, Plant CellReports 15:677-681). In this method, the standard deviation of codonusage, a measure of codon usage bias, may be calculated by first findingthe squared proportional deviation of usage of each codon of the nativegene relative to that of highly expressed plant genes, followed by acalculation of the average squared deviation. The formula used is: 1SDCU=n=1 N [(Xn−Yn)/Yn]2/N, where Xn refers to the frequency of usage ofcodon n in highly expressed plant genes, where Yn to the frequency ofusage of codon n in the gene of interest and N refers to the totalnumber of codons in the gene of interest. A Table of codon usage fromhighly expressed genes of dicotyledonous plants is compiled using thedata of Murray et al. (1989, Nuc Acids Res. 17:477-498).

One method of optimizing the nucleic acid sequence in accordance withthe preferred codon usage for a particular plant cell type is based onthe direct use, without performing any extra statistical calculations,of codon optimization Tables such as those provided on-line at the CodonUsage Database through the NIAS (National Institute of AgrobiologicalSciences) DNA bank in Japan (Hypertext Transfer Protocol://World WideWeb (dot) kazusa (dot) or (dot) jp/codon/). The Codon Usage Databasecontains codon usage tables for a number of different species, with eachcodon usage Table having been statistically determined based on the datapresent in Genbank.

By using the above Tables to determine the most preferred or mostfavored codons for each amino acid in a particular species (for example,rice), a naturally-occurring nucleotide sequence encoding a protein ofinterest can be codon optimized for that particular plant species. Thisis effected by replacing codons that may have a low statisticalincidence in the particular species genome with corresponding codons, inregard to an amino acid, that are statistically more favored. However,one or more less-favored codons may be selected to delete existingrestriction sites, to create new ones at potentially useful junctions(5′ and 3′ ends to add signal peptide or termination cassettes, internalsites that might be used to cut and splice segments together to producea correct full-length sequence), or to eliminate nucleotide sequencesthat may negatively effect mRNA stability or expression.

The naturally-occurring encoding nucleotide sequence may already, inadvance of any modification, contain a number of codons that correspondto a statistically-favored codon in a particular plant species.Therefore, codon optimization of the native nucleotide sequence maycomprise determining which codons, within the native nucleotidesequence, are not statistically-favored with regards to a particularplant, and modifying these codons in accordance with a codon usage tableof the particular plant to produce a codon optimized derivative. Amodified nucleotide sequence may be fully or partially optimized forplant codon usage provided that the protein encoded by the modifiednucleotide sequence is produced at a level higher than the proteinencoded by the corresponding naturally occurring or native gene.Construction of synthetic genes by altering the codon usage is describedin for example PCT Patent Application 93/07278.

According to some embodiments of the invention, the exogenouspolynucleotide is a non-coding RNA.

As used herein the phrase ‘non-coding RNA” refers to an RNA moleculewhich does not encode an amino acid sequence (a polypeptide). Examplesof such non-coding RNA molecules include, but are not limited to, anantisense RNA, a pre-miRNA (precursor of a microRNA), or a precursor ofa Piwi-interacting RNA (piRNA).

Non-limiting examples of non-coding RNA polynucleotides are provided inSEQ ID NOs: 201-213, 280-288, and 476-480.

Thus, the invention encompasses nucleic acid sequences describedhereinabove; fragments thereof, sequences hybridizable therewith,sequences homologous thereto, sequences encoding similar polypeptideswith different codon usage, altered sequences characterized bymutations, such as deletion, insertion or substitution of one or morenucleotides, either naturally occurring or man induced, either randomlyor in a targeted fashion.

The invention provides an isolated polynucleotide comprising a nucleicacid sequence at least about 80%, at least about 81%, at least about82%, at least about 83%, at least about 84%, at least about 85%, atleast about 86%, at least about 87%, at least about 88%, at least about89%, at least about 90%, at least about 91%, at least about 92%, atleast about 93%, at least about 93%, at least about 94%, at least about95%, at least about 96%, at least about 97%, at least about 98%, atleast about 99%, e.g., 100% identical to the polynucleotide selectedfrom the group consisting of SEQ ID NOs:1-480, and 793-2946.

According to some embodiments of the invention the nucleic acid sequenceis capable of increasing yield, biomass, growth rate, vigor, oilcontent, fiber yield, fiber quality, abiotic stress tolerance,fertilizer use efficiency, water use efficiency and/or nitrogen useefficiency of a plant.

According to some embodiments of the invention the isolatedpolynucleotide comprising the nucleic acid sequence selected from thegroup consisting of SEQ ID NOs:1-480, and 793-2946.

According to some embodiments of the invention the isolatedpolynucleotide is set forth by SEQ ID NO:1-480, 793-2945 or 2946.

The invention provides an isolated polynucleotide comprising a nucleicacid sequence encoding a polypeptide which comprises an amino acidsequence at least about 80%, at least about 81%, at least about 82%, atleast about 83%, at least about 84%, at least about 85%, at least about86%, at least about 87%, at least about 88%, at least about 89%, atleast about 90%, at least about 91%, at least about 92%, at least about93%, at least about 93%, at least about 94%, at least about 95%, atleast about 96%, at least about 97%, at least about 98%, at least about99%, or more say 100% homologous to the amino acid sequence selectedfrom the group consisting of SEQ ID NO: 481-792, and 2947-4663.

According to some embodiments of the invention the amino acid sequenceis capable of increasing yield, biomass, growth rate, vigor, oilcontent, fiber yield, fiber quality, abiotic stress tolerance,fertilizer use efficiency, water use efficiency and/or nitrogen useefficiency of a plant.

The invention provides an isolated polynucleotide comprising a nucleicacid sequence encoding a polypeptide which comprises the amino acidsequence selected from the group consisting of SEQ ID NOs:481-792, and2947-4663.

The invention provides an isolated polypeptide comprising an amino acidsequence at least about 80%, at least about 81%, at least about 82%, atleast about 83%, at least about 84%, at least about 85%, at least about86%, at least about 87%, at least about 88%, at least about 89%, atleast about 90%, at least about 91%, at least about 92%, at least about93%, at least about 93%, at least about 94%, at least about 95%, atleast about 96%, at least about 97%, at least about 98%, at least about99%, or more say 100% homologous to an amino acid sequence selected fromthe group consisting of SEQ ID NO: 481-792, and 2947-4663.

According to some embodiments of the invention the amino acid sequenceis capable of increasing yield, biomass, growth rate, vigor, oilcontent, fiber yield, fiber quality, abiotic stress tolerance,fertilizer use efficiency, water use efficiency and/or nitrogen useefficiency of a plant.

According to some embodiments of the invention, the polypeptidecomprising an amino acid sequence selected from the group consisting ofSEQ ID NOs:481-792, and 2947-4663.

According to some embodiments of the invention, the polypeptide is setforth by SEQ ID NO: 481-792, 2947-4662 or 4663.

According to an aspect of some embodiments of the invention, there isprovided a nucleic acid construct comprising the isolated polynucleotideof the invention, and a promoter for directing transcription of thenucleic acid sequence in a host cell.

The invention also encompasses fragments of the above describedpolypeptides and polypeptides having mutations, such as deletions,insertions or substitutions of one or more amino acids, either naturallyoccurring or man induced, either randomly or in a targeted fashion.

The term “plant” as used herein encompasses whole plants, ancestors andprogeny of the plants and plant parts, including seeds, shoots, stems,roots (including tubers), and plant cells, tissues and organs. The plantmay be in any form including suspension cultures, embryos, meristematicregions, callus tissue, leaves, gametophytes, sporophytes, pollen, andmicrospores. Plants that are particularly useful in the methods of theinvention include all plants which belong to the superfamilyViridiplantae, in particular monocotyledonous and dicotyledonous plantsincluding a fodder or forage legume, ornamental plant, food crop, tree,or shrub selected from the list comprising Acacia spp., Acer spp.,Actinidia spp., Aesculus spp., Agathis australis, Albizia amara,Alsophila tricolor, Andropogon spp., Arachis spp, Areca catechu, Asteliafragrans, Astragalus cicer, Baikiaea plurijuga, Betula spp., Brassicaspp., Bruguiera gymnorrhiza, Burkea africana, Butea frondosa, Cadabafarinosa, Calliandra spp, Camellia sinensis, Canna indica, Capsicumspp., Cassia spp., Centroema pubescens, Chacoomeles spp., Cinnamomumcassia, Coffea arabica, Colophospermum mopane, Coronillia varia,Cotoneaster serotina, Crataegus spp., Cucumis spp., Cupressus spp.,Cyathea dealbata, Cydonia oblonga, Cryptomeria japonica, Cymbopogonspp., Cynthea dealbata, Cydonia oblonga, Dalbergia monetaria, Davalliadivaricata, Desmodium spp., Dicksonia squarosa, Dibeteropogonamplectens, Dioclea spp, Dolichos spp., Dorycnium rectum, Echinochloapyramidalis, Ehraffia spp., Eleusine coracana, Eragrestis spp.,Erythrina spp., Eucalypfus spp., Euclea schimperi, Eulalia vi/losa,Pagopyrum spp., Feijoa sellowlana, Fragaria spp., Flemingia spp,Freycinetia banksli, Geranium thunbergii, GinAgo biloba, Glycinejavanica, Gliricidia spp, Gossypium hirsutum, Grevillea spp., Guibourtiacoleosperma, Hedysarum spp., Hemaffhia altissima, Heteropogon contoffus,Hordeum vulgare, Hyparrhenia rufa, Hypericum erectum, Hypeffheliadissolute, Indigo incamata, Iris spp., Leptarrhena pyrolifolia,Lespediza spp., Lettuca spp., Leucaena leucocephala, Loudetia simplex,Lotonus bainesli, Lotus spp., Macrotyloma axillare, Malus spp., Manihotesculenta, Medicago saliva, Metasequoia glyptostroboides, Musasapientum, Nicotianum spp., Onobrychis spp., Ornithopus spp., Oryzaspp., Peltophorum africanum, Pennisetum spp., Persea gratissima, Petuniaspp., Phaseolus spp., Phoenix canariensis, Phormium cookianum, Photiniaspp., Picea glauca, Pinus spp., Pisum sativam, Podocarpus totara,Pogonarthria fleckii, Pogonaffhria squarrosa, Populus spp., Prosopiscineraria, Pseudotsuga menziesii, Pterolobium stellatum, Pyrus communis,Quercus spp., Rhaphiolepsis umbellata, Rhopalostylis sapida, Rhusnatalensis, Ribes grossularia, Ribes spp., Robinia pseudoacacia, Rosaspp., Rubus spp., Salix spp., Schyzachyrium sanguineum, Sciadopitysvefficillata, Sequoia sempervirens, Sequoiadendron giganteum, Sorghumbicolor, Spinacia spp., Sporobolus fimbriatus, Stiburus alopecuroides,Stylosanthos humilis, Tadehagi spp, Taxodium distichum, Themedatriandra, Trifolium spp., Triticum spp., Tsuga heterophylla, Vacciniumspp., Vicia spp., Vitis vinifera, Watsonia pyramidata, Zantedeschiaaethiopica, Zea mays, amaranth, artichoke, asparagus, broccoli, Brusselssprouts, cabbage, canola, carrot, cauliflower, celery, collard greens,flax, kale, lentil, oilseed rape, okra, onion, potato, rice, soybean,straw, sugar beet, sugar cane, sunflower, tomato, squash tea, maize,wheat, barely, rye, oat, peanut, pea, lentil and alfalfa, cotton,rapeseed, canola, pepper, sunflower, tobacco, eggplant, eucalyptus, atree, an ornamental plant, a perennial grass and a forage crop.Alternatively algae and other non-Viridiplantae can be used for themethods of the present invention.

According to some embodiments of the invention, the plant used by themethod of the invention is a crop plant such as rice, maize, wheat,barley, peanut, potato, sesame, olive tree, palm oil, banana, soybean,sunflower, canola, sugarcane, alfalfa, millet, leguminosae (bean, pea),flax, lupinus, rapeseed, tobacco, poplar and cotton.

According to some embodiments of the invention, there is provided aplant cell exogenously expressing the polynucleotide of some embodimentsof the invention, the nucleic acid construct of some embodiments of theinvention and/or the polypeptide of some embodiments of the invention.

According to some embodiments of the invention, expressing the exogenouspolynucleotide of the invention within the plant is effected bytransforming one or more cells of the plant with the exogenouspolynucleotide, followed by generating a mature plant from thetransformed cells and cultivating the mature plant under conditionssuitable for expressing the exogenous polynucleotide within the matureplant.

According to some embodiments of the invention, the transformation iseffected by introducing to the plant cell a nucleic acid construct whichincludes the exogenous polynucleotide of some embodiments of theinvention and at least one promoter for directing transcription of theexogenous polynucleotide in a host cell (a plant cell). Further detailsof suitable transformation approaches are provided hereinbelow.

As mentioned, the nucleic acid construct according to some embodimentsof the invention comprises a promoter sequence and the isolatedpolynucleotide of the invention.

According to some embodiments of the invention, the isolatedpolynucleotide is operably linked to the promoter sequence.

A coding nucleic acid sequence is “operably linked” to a regulatorysequence (e.g., promoter) if the regulatory sequence is capable ofexerting a regulatory effect on the coding sequence linked thereto.

As used herein, the term “promoter” refers to a region of DNA which liesupstream of the transcriptional initiation site of a gene to which RNApolymerase binds to initiate transcription of RNA. The promoter controlswhere (e.g., which portion of a plant) and/or when (e.g., at which stageor condition in the lifetime of an organism) the gene is expressed.

According to some embodiments of the invention, the promoter isheterologous to the isolated polynucleotide and/or to the host cell.

Any suitable promoter sequence can be used by the nucleic acid constructof the present invention. Preferably the promoter is a constitutivepromoter, a tissue-specific, or an abiotic stress-inducible promoter.

According to some embodiments of the invention, the promoter is a plantpromoter, which is suitable for expression of the exogenouspolynucleotide in a plant cell.

Suitable constitutive promoters include, for example, CaMV 35S promoter[SEQ ID NO:4666 (pQFNC); SEQ ID NO:5158 (PJJ 35S from Brachypodium); SEQID NO:5159 (Odell et al., Nature 313:810-812, 1985)], Arabidopsis At6669promoter (SEQ ID NO:4665; see PCT Publication No. W004081173A2 or thenew At6669 promoter (SEQ ID NO:4668); maize Ubi 1 (Christensen et al.,Plant Sol. Biol. 18:675-689, 1992); rice actin (McElroy et al., PlantCell 2:163-171, 1990); pEMU (Last et al., Theor. Appl. Genet.81:581-588, 1991); CaMV 19S (Nilsson et al., Physiol. Plant 100:456-462,1997); GOS2 (de Pater et al, Plant J November; 2(6):837-44, 1992);ubiquitin (Christensen et al, Plant Mol. Biol. 18: 675-689, 1992); Ubi 1promoter (SEQ ID NO:5157); RBCS promoter (SEQ ID NO:5156); Ricecyclophilin (Bucholz et al, Plant Mol Biol. 25(5):837-43, 1994); MaizeH3 histone (Lepetit et al, Mol. Gen. Genet. 231: 276-285, 1992); Actin 2(An et al, Plant J. 10(1); 107-121, 1996) and Synthetic Super MAS (Ni etal., The Plant Journal 7: 661-76, 1995). Other constitutive promotersinclude those in U.S. Pat. Nos. 5,659,026, 5,608,149; 5,608,144;5,604,121; 5,569,597: 5,466,785; 5,399,680; 5,268,463; and 5,608,142.

Suitable tissue-specific promoters include, but not limited to,leaf-specific promoters [such as described, for example, by Yamamoto etal., Plant J. 12:255-265, 1997; Kwon et al., Plant Physiol. 105:357-67,1994; Yamamoto et al., Plant Cell Physiol. 35:773-778, 1994; Gotor etal., Plant J. 3:509-18, 1993; Orozco et al., Plant Mol. Biol.23:1129-1138, 1993; and Matsuoka et al., Proc. Natl. Acad. Sci. USA90:9586-9590, 1993], seed-preferred promoters [e.g., Napin (originatedfrom Brassica napus which is characterized by a seed specific promoteractivity; Stuitje A. R. et. al. Plant Biotechnology Journal 1 (4):301-309; SEQ ID NO:4667), from seed specific genes (Simon, et al., PlantMol. Biol. 5. 191, 1985; Scofield, et al., J. Biol. Chem. 262: 12202,1987; Baszczynski, et al., Plant Mol. Biol. 14: 633, 1990), Brazil Nutalbumin (Pearson' et al., Plant Mol. Biol. 18: 235-245, 1992), legumin(Ellis, et al. Plant Mol. Biol. 10: 203-214, 1988), Glutelin (rice)(Takaiwa, et al., Mol. Gen. Genet. 208: 15-22, 1986; Takaiwa, et al.,FEBS Letts. 221: 43-47, 1987), Zein (Matzke et al Plant Mol Biol, 143).323-32 1990), napA (Stalberg, et al, Planta 199: 515-519, 1996), WheatSPA (Albanietal, Plant Cell, 9: 171-184, 1997), sunflower oleosin(Cummins, etal., Plant Mol. Biol. 19: 873-876, 1992)], endospermspecific promoters [e.g., wheat LMW and HMW, glutenin-1 (Mol Gen Genet216:81-90, 1989; NAR 17:461-2), wheat a, b and g gliadins(EMBO3:1409-15, 1984), Barley ltrl promoter, barley B1, C, D hordein(Theor Appl Gen 98:1253-62, 1999; Plant J 4:343-55, 1993; Mol Gen Genet250:750-60, 1996), Barley DOF (Mena et al, The Plant Journal, 116(1):53-62, 1998), Biz2 (EP99106056.7), Synthetic promoter (Vicente-Carbajosaet al., Plant J. 13: 629-640, 1998), rice prolamin NRP33, rice-globulinGlb-1 (Wu et al, Plant Cell Physiology 39(8) 885-889, 1998), ricealpha-globulin REB/OHP-1 (Nakase et al. Plant Mol. Biol. 33: 513-S22,1997), rice ADP-glucose PP (Trans Res 6:157-68, 1997), maize ESR genefamily (Plant J 12:235-46, 1997), sorgum gamma-kafirin (PMB 32:1029-35,1996)], embryo specific promoters [e.g., rice OSH1 (Sato et al, Proc.Nati. Acad. Sci. USA, 93: 8117-8122), KNOX (Postma-Haarsma of al, PlantMol. Biol. 39:257-71, 1999), rice oleosin (Wu et at, J. Biochem.,123:386, 1998)], and flower-specific promoters [e.g., AtPRP4, chalenesynthase (chsA) (Van der Meer, et al., Plant Mol. Biol. 15, 95-109,1990), LAT52 (Twell et al Mol. Gen Genet. 217:240-245; 1989),apetala—3], and root promoters such as the ROOTP promoter [SEQ ID NO:4669].

Suitable abiotic stress-inducible promoters include, but not limited to,salt-inducible promoters such as RD29A (Yamaguchi-Shinozalei et al.,Mol. Gen. Genet. 236:331-340, 1993); drought-inducible promoters such asmaize rab17 gene promoter (Pla et. al., Plant Mol. Biol. 21:259-266,1993), maize rab28 gene promoter (Busk et. al., Plant J. 11:1285-1295,1997) and maize Ivr2 gene promoter (Pelleschi et. al., Plant Mol. Biol.39:373-380, 1999); heat-inducible promoters such as heat tomatohsp80-promoter from tomato (U.S. Pat. No. 5,187,267).

The nucleic acid construct of some embodiments of the invention canfurther include an appropriate selectable marker and/or an origin ofreplication. According to some embodiments of the invention, the nucleicacid construct utilized is a shuttle vector, which can propagate both inE. coli (wherein the construct comprises an appropriate selectablemarker and origin of replication) and be compatible with propagation incells. The construct according to the present invention can be, forexample, a plasmid, a bacmid, a phagemid, a cosmid, a phage, a virus oran artificial chromosome.

The nucleic acid construct of some embodiments of the invention can beutilized to stably or transiently transform plant cells. In stabletransformation, the exogenous polynucleotide is integrated into theplant genome and as such it represents a stable and inherited trait. Intransient transformation, the exogenous polynucleotide is expressed bythe cell transformed but it is not integrated into the genome and assuch it represents a transient trait.

There are various methods of introducing foreign genes into bothmonocotyledonous and dicotyledonous plants (Potrykus, I., Annu. Rev.Plant. Physiol., Plant. Mol. Biol. (1991) 42:205-225; Shimamoto et al.,Nature (1989) 338:274-276).

The principle methods of causing stable integration of exogenous DNAinto plant genomic DNA include two main approaches:

(i) Agrobacterium-mediated gene transfer: Klee et al. (1987) Annu. Rev.Plant Physiol. 38:467-486; Klee and Rogers in Cell Culture and SomaticCell Genetics of Plants, Vol. 6, Molecular Biology of Plant NuclearGenes, eds. Schell, J., and Vasil, L. K., Academic Publishers, SanDiego, Calif. (1989) p. 2-25; Gatenby, in Plant Biotechnology, eds.Kung, S. and Arntzen, C. J., Butterworth Publishers, Boston, Mass.(1989) p. 93-112.

(ii) Direct DNA uptake: Paszkowski et al., in Cell Culture and SomaticCell Genetics of Plants, Vol. 6, Molecular Biology of Plant NuclearGenes eds. Schell, J., and Vasil, L. K., Academic Publishers, San Diego,Calif. (1989) p. 52-68; including methods for direct uptake of DNA intoprotoplasts, Toriyama, K. et al. (1988) Bio/Technology 6:1072-1074. DNAuptake induced by brief electric shock of plant cells: Zhang et al.Plant Cell Rep. (1988) 7:379-384. Fromm et al. Nature (1986)319:791-793. DNA injection into plant cells or tissues by particlebombardment, Klein et al. Bio/Technology (1988) 6:559-563; McCabe et al.Bio/Technology (1988) 6:923-926; Sanford, Physiol. Plant. (1990)79:206-209; by the use of micropipette systems: Neuhaus et al., Theor.Appl. Genet. (1987) 75:30-36; Neuhaus and Spangenberg, Physiol. Plant.(1990) 79:213-217; glass fibers or silicon carbide whiskertransformation of cell cultures, embryos or callus tissue, U.S. Pat. No.5,464,765 or by the direct incubation of DNA with germinating pollen,DeWet et al. in Experimental Manipulation of Ovule Tissue, eds. Chapman,G. P. and Mantell, S. H. and Daniels, W. Longman, London, (1985) p.197-209; and Ohta, Proc. Natl. Acad. Sci. USA (1986) 83:715-719.

The Agrobacterium system includes the use of plasmid vectors thatcontain defined DNA segments that integrate into the plant genomic DNA.Methods of inoculation of the plant tissue vary depending upon the plantspecies and the Agrobacterium delivery system. A widely used approach isthe leaf disc procedure which can be performed with any tissue explantthat provides a good source for initiation of whole plantdifferentiation. See, e.g., Horsch et al. in Plant Molecular BiologyManual A5, Kluwer Academic Publishers, Dordrecht (1988) p. 1-9. Asupplementary approach employs the Agrobacterium delivery system incombination with vacuum infiltration. The Agrobacterium system isespecially viable in the creation of transgenic dicotyledonous plants.

There are various methods of direct DNA transfer into plant cells. Inelectroporation, the protoplasts are briefly exposed to a strongelectric field. In microinjection, the DNA is mechanically injecteddirectly into the cells using very small micropipettes. In microparticlebombardment, the DNA is adsorbed on microprojectiles such as magnesiumsulfate crystals or tungsten particles, and the microprojectiles arephysically accelerated into cells or plant tissues.

Following stable transformation plant propagation is exercised. The mostcommon method of plant propagation is by seed. Regeneration by seedpropagation, however, has the deficiency that due to heterozygositythere is a lack of uniformity in the crop, since seeds are produced byplants according to the genetic variances governed by Mendelian rules.Basically, each seed is genetically different and each will grow withits own specific traits. Therefore, it is preferred that the transformedplant be produced such that the regenerated plant has the identicaltraits and characteristics of the parent transgenic plant. Therefore, itis preferred that the transformed plant be regenerated bymicropropagation which provides a rapid, consistent reproduction of thetransformed plants.

Micropropagation is a process of growing new generation plants from asingle piece of tissue that has been excised from a selected parentplant or cultivar. This process permits the mass reproduction of plantshaving the preferred tissue expressing the fusion protein. The newgeneration plants which are produced are genetically identical to, andhave all of the characteristics of, the original plant. Micropropagationallows mass production of quality plant material in a short period oftime and offers a rapid multiplication of selected cultivars in thepreservation of the characteristics of the original transgenic ortransformed plant. The advantages of cloning plants are the speed ofplant multiplication and the quality and uniformity of plants produced.

Micropropagation is a multi-stage procedure that requires alteration ofculture medium or growth conditions between stages. Thus, themicropropagation process involves four basic stages: Stage one, initialtissue culturing; stage two, tissue culture multiplication; stage three,differentiation and plant formation; and stage four, greenhouseculturing and hardening. During stage one, initial tissue culturing, thetissue culture is established and certified contaminant-free. Duringstage two, the initial tissue culture is multiplied until a sufficientnumber of tissue samples are produced to meet production goals. Duringstage three, the tissue samples grown in stage two are divided and growninto individual plantlets. At stage four, the transformed plantlets aretransferred to a greenhouse for hardening where the plants' tolerance tolight is gradually increased so that it can be grown in the naturalenvironment.

According to some embodiments of the invention, the transgenic plantsare generated by transient transformation of leaf cells, meristematiccells or the whole plant. Transient transformation can be effected byany of the direct DNA transfer methods described above or by viralinfection using modified plant viruses.

Viruses that have been shown to be useful for the transformation ofplant hosts include CaMV, Tobacco mosaic virus (TMV), brome mosaic virus(BMV) and Bean Common Mosaic Virus (BV or BCMV). Transformation ofplants using plant viruses is described in U.S. Pat. No. 4,855,237 (beangolden mosaic virus; BGV), EP-A 67,553 (TMV), Japanese PublishedApplication No. 63-14693 (TMV), EPA 194,809 (BV), EPA 278,667 (BV); andGluzman, Y. et al., Communications in Molecular Biology: Viral Vectors,Cold Spring Harbor Laboratory, New York, pp. 172-189 (1988). Pseudovirusparticles for use in expressing foreign DNA in many hosts, includingplants are described in WO 87/06261.

According to some embodiments of the invention, the virus used fortransient transformations is avirulent and thus is incapable of causingsevere symptoms such as reduced growth rate, mosaic, ring spots, leafroll, yellowing, streaking, pox formation, tumor formation and pitting.A suitable avirulent virus may be a naturally occurring avirulent virusor an artificially attenuated virus. Virus attenuation may be effectedby using methods well known in the art including, but not limited to,sub-lethal heating, chemical treatment or by directed mutagenesistechniques such as described, for example, by Kurihara and Watanabe(Molecular Plant Pathology 4:259-269, 2003), Gal-on et al. (1992),Atreya et al. (1992) and Huet et al. (1994).

Suitable virus strains can be obtained from available sources such as,for example, the American Type culture Collection (ATCC) or by isolationfrom infected plants. Isolation of viruses from infected plant tissuescan be effected by techniques well known in the art such as described,for example by Foster and Tatlor, Eds. “Plant Virology Protocols: FromVirus Isolation to Transgenic Resistance (Methods in Molecular Biology(Humana Pr), Vol 81)”, Humana Press, 1998. Briefly, tissues of aninfected plant believed to contain a high concentration of a suitablevirus, preferably young leaves and flower petals, are ground in a buffersolution (e.g., phosphate buffer solution) to produce a virus infectedsap which can be used in subsequent inoculations.

Construction of plant RNA viruses for the introduction and expression ofnon-viral exogenous polynucleotide sequences in plants is demonstratedby the above references as well as by Dawson, W. O. et al., Virology(1989) 172:285-292; Takamatsu et al. EMBO J. (1987) 6:307-311; French etal. Science (1986) 231:1294-1297; Takamatsu et al. FEBS Letters (1990)269:73-76; and U.S. Pat. No. 5,316,931.

When the virus is a DNA virus, suitable modifications can be made to thevirus itself. Alternatively, the virus can first be cloned into abacterial plasmid for ease of constructing the desired viral vector withthe foreign DNA. The virus can then be excised from the plasmid. If thevirus is a DNA virus, a bacterial origin of replication can be attachedto the viral DNA, which is then replicated by the bacteria.Transcription and translation of this DNA will produce the coat proteinwhich will encapsidate the viral DNA. If the virus is an RNA virus, thevirus is generally cloned as a cDNA and inserted into a plasmid. Theplasmid is then used to make all of the constructions. The RNA virus isthen produced by transcribing the viral sequence of the plasmid andtranslation of the viral genes to produce the coat protein(s) whichencapsidate the viral RNA.

In one embodiment, a plant viral polynucleotide is provided in which thenative coat protein coding sequence has been deleted from a viralpolynucleotide, a non-native plant viral coat protein coding sequenceand a non-native promoter, preferably the subgenomic promoter of thenon-native coat protein coding sequence, capable of expression in theplant host, packaging of the recombinant plant viral polynucleotide, andensuring a systemic infection of the host by the recombinant plant viralpolynucleotide, has been inserted. Alternatively, the coat protein genemay be inactivated by insertion of the non-native polynucleotidesequence within it, such that a protein is produced. The recombinantplant viral polynucleotide may contain one or more additional non-nativesubgenomic promoters. Each non-native subgenomic promoter is capable oftranscribing or expressing adjacent genes or polynucleotide sequences inthe plant host and incapable of recombination with each other and withnative subgenomic promoters. Non-native (foreign) polynucleotidesequences may be inserted adjacent the native plant viral subgenomicpromoter or the native and a non-native plant viral subgenomic promotersif more than one polynucleotide sequence is included. The non-nativepolynucleotide sequences are transcribed or expressed in the host plantunder control of the subgenomic promoter to produce the desiredproducts.

In a second embodiment, a recombinant plant viral polynucleotide isprovided as in the first embodiment except that the native coat proteincoding sequence is placed adjacent one of the non-native coat proteinsubgenomic promoters instead of a non-native coat protein codingsequence.

In a third embodiment, a recombinant plant viral polynucleotide isprovided in which the native coat protein gene is adjacent itssubgenomic promoter and one or more non-native subgenomic promoters havebeen inserted into the viral polynucleotide. The inserted non-nativesubgenomic promoters are capable of transcribing or expressing adjacentgenes in a plant host and are incapable of recombination with each otherand with native subgenomic promoters. Non-native polynucleotidesequences may be inserted adjacent the non-native subgenomic plant viralpromoters such that the sequences are transcribed or expressed in thehost plant under control of the subgenomic promoters to produce thedesired product.

In a fourth embodiment, a recombinant plant viral polynucleotide isprovided as in the third embodiment except that the native coat proteincoding sequence is replaced by a non-native coat protein codingsequence.

The viral vectors are encapsidated by the coat proteins encoded by therecombinant plant viral polynucleotide to produce a recombinant plantvirus. The recombinant plant viral polynucleotide or recombinant plantvirus is used to infect appropriate host plants. The recombinant plantviral polynucleotide is capable of replication in the host, systemicspread in the host, and transcription or expression of foreign gene(s)(exogenous polynucleotide) in the host to produce the desired protein.

Techniques for inoculation of viruses to plants may be found in Fosterand Taylor, eds. “Plant Virology Protocols: From Virus Isolation toTransgenic Resistance (Methods in Molecular Biology (Humana Pr), Vol81)”, Humana Press, 1998; Maramorosh and Koprowski, eds. “Methods inVirology” 7 vols, Academic Press, New York 1967-1984; Hill, S. A.“Methods in Plant Virology”, Blackwell, Oxford, 1984; Walkey, D. G. A.“Applied Plant Virology”, Wiley, New York, 1985; and Kado and Agrawa,eds. “Principles and Techniques in Plant Virology”, VanNostrand-Reinhold, New York.

In addition to the above, the polynucleotide of the present inventioncan also be introduced into a chloroplast genome thereby enablingchloroplast expression.

A technique for introducing exogenous polynucleotide sequences to thegenome of the chloroplasts is known. This technique involves thefollowing procedures. First, plant cells are chemically treated so as toreduce the number of chloroplasts per cell to about one. Then, theexogenous polynucleotide is introduced via particle bombardment into thecells with the aim of introducing at least one exogenous polynucleotidemolecule into the chloroplasts. The exogenous polynucleotides selectedsuch that it is integratable into the chloroplast's genome viahomologous recombination which is readily effected by enzymes inherentto the chloroplast. To this end, the exogenous polynucleotide includes,in addition to a gene of interest, at least one polynucleotide stretchwhich is derived from the chloroplast's genome. In addition, theexogenous polynucleotide includes a selectable marker, which serves bysequential selection procedures to ascertain that all or substantiallyall of the copies of the chloroplast genomes following such selectionwill include the exogenous polynucleotide. Further details relating tothis technique are found in U.S. Pat. Nos. 4,945,050; and 5,693,507which are incorporated herein by reference. A polypeptide can thus beproduced by the protein expression system of the chloroplast and becomeintegrated into the chloroplast's inner membrane.

Since processes which increase yield, biomass, growth rate, vigor, oilcontent, fiber yield, fiber quality, abiotic stress tolerance, and/ornitrogen use efficiency of a plant can involve multiple genes actingadditively or in synergy (see, for example, in Quesda et al., PlantPhysiol. 130:951-063, 2002), the present invention also envisagesexpressing a plurality of exogenous polynucleotides in a single hostplant to thereby achieve superior effect on oil content, yield, growthrate, biomass, vigor and/or abiotic stress tolerance.

Expressing a plurality of exogenous polynucleotides in a single hostplant can be effected by co-introducing multiple nucleic acidconstructs, each including a different exogenous polynucleotide, into asingle plant cell. The transformed cell can than be regenerated into amature plant using the methods described hereinabove.

Alternatively, expressing a plurality of exogenous polynucleotides in asingle host plant can be effected by co-introducing into a singleplant-cell a single nucleic-acid construct including a plurality ofdifferent exogenous polynucleotides. Such a construct can be designedwith a single promoter sequence which can transcribe a polycistronicmessenger RNA including all the different exogenous polynucleotidesequences. To enable co-translation of the different polypeptidesencoded by the polycistronic messenger RNA, the polynucleotide sequencescan be inter-linked via an internal ribosome entry site (IRES) sequencewhich facilitates translation of polynucleotide sequences positioneddownstream of the IRES sequence. In this case, a transcribedpolycistronic RNA molecule encoding the different polypeptides describedabove will be translated from both the capped 5′ end and the twointernal IRES sequences of the polycistronic RNA molecule to therebyproduce in the cell all different polypeptides. Alternatively, theconstruct can include several promoter sequences each linked to adifferent exogenous polynucleotide sequence.

The plant cell transformed with the construct including a plurality ofdifferent exogenous polynucleotides, can be regenerated into a matureplant, using the methods described hereinabove.

Alternatively, expressing a plurality of exogenous polynucleotides in asingle host plant can be effected by introducing different nucleic acidconstructs, including different exogenous polynucleotides, into aplurality of plants. The regenerated transformed plants can then becross-bred and resultant progeny selected for superior abiotic stresstolerance, water use efficiency, fertilizer use efficiency, growth,biomass, yield and/or vigor traits, using conventional plant breedingtechniques.

According to some embodiments of the invention, the method furthercomprising growing the plant expressing the exogenous polynucleotideunder the abiotic stress.

Non-limiting examples of abiotic stress conditions include, salinity,drought, water deprivation, excess of water (e.g., flood, waterlogging),etiolation, low temperature, high temperature, heavy metal toxicity,anaerobiosis, nutrient deficiency, nutrient excess, atmosphericpollution and UV irradiation.

Thus, the invention encompasses plants exogenously expressing thepolynucleotide(s), the nucleic acid constructs and/or polypeptide(s) ofthe invention.

Once expressed within the plant cell or the entire plant, the level ofthe polypeptide encoded by the exogenous polynucleotide can bedetermined by methods well known in the art such as, activity assays,Western blots using antibodies capable of specifically binding thepolypeptide, Enzyme-Linked Immuno Sorbent Assay (ELISA),radio-immuno-assays (RIA), immunohistochemistry, immunocytochemistry,immunofluorescence and the like.

Methods of determining the level in the plant of the RNA transcribedfrom the exogenous polynucleotide are well known in the art and include,for example, Northern blot analysis, reverse transcription polymerasechain reaction (RT-PCR) analysis (including quantitative,semi-quantitative or real-time RT-PCR) and RNA-in situ hybridization.

The sequence information and annotations uncovered by the presentteachings can be harnessed in favor of classical breeding. Thus,sub-sequence data of those polynucleotides described above, can be usedas markers for marker assisted selection (MAS), in which a marker isused for indirect selection of a genetic determinant or determinants ofa trait of interest (e.g., biomass, growth rate, oil content, yield,abiotic stress tolerance, water use efficiency, nitrogen use efficiencyand/or fertilizer use efficiency). Nucleic acid data of the presentteachings (DNA or RNA sequence) may contain or be linked to polymorphicsites or genetic markers on the genome such as restriction fragmentlength polymorphism (RFLP), microsatellites and single nucleotidepolymorphism (SNP), DNA fingerprinting (DFP), amplified fragment lengthpolymorphism (AFLP), expression level polymorphism, polymorphism of theencoded polypeptide and any other polymorphism at the DNA or RNAsequence.

Examples of marker assisted selections include, but are not limited to,selection for a morphological trait (e.g., a gene that affects form,coloration, male sterility or resistance such as the presence or absenceof awn, leaf sheath coloration, height, grain color, aroma of rice);selection for a biochemical trait (e.g., a gene that encodes a proteinthat can be extracted and observed; for example, isozymes and storageproteins); selection for a biological trait (e.g., pathogen races orinsect biotypes based on host pathogen or host parasite interaction canbe used as a marker since the genetic constitution of an organism canaffect its susceptibility to pathogens or parasites).

The polynucleotides and polypeptides described hereinabove can be usedin a wide range of economical plants, in a safe and cost effectivemanner.

Plant lines exogenously expressing the polynucleotide or the polypeptideof the invention are screened to identify those that show the greatestincrease of the desired plant trait.

The effect of the transgene (the exogenous polynucleotide encoding thepolypeptide) on abiotic stress tolerance can be determined using knownmethods such as detailed below and in the Examples section whichfollows.

Abiotic Stress Tolerance—

Transformed (i.e., expressing the transgene) and non-transformed (wildtype) plants are exposed to an abiotic stress condition, such as waterdeprivation, suboptimal temperature (low temperature, high temperature),nutrient deficiency, nutrient excess, a salt stress condition, osmoticstress, heavy metal toxicity, anaerobiosis, atmospheric pollution and UVirradiation.

Salinity Tolerance Assay—

Transgenic plants with tolerance to high salt concentrations areexpected to exhibit better germination, seedling vigor or growth in highsalt. Salt stress can be effected in many ways such as, for example, byirrigating the plants with a hyperosmotic solution, by cultivating theplants hydroponically in a hyperosmotic growth solution (e.g., Hoaglandsolution), or by culturing the plants in a hyperosmotic growth medium[e.g., 50% Murashige-Skoog medium (MS medium)]. Since different plantsvary considerably in their tolerance to salinity, the salt concentrationin the irrigation water, growth solution, or growth medium can beadjusted according to the specific characteristics of the specific plantcultivar or variety, so as to inflict a mild or moderate effect on thephysiology and/or morphology of the plants (for guidelines as toappropriate concentration see, Bernstein and Kafkafi, Root Growth UnderSalinity Stress In: Plant Roots, The Hidden Half 3rd ed. Waisel Y, EshelA and Kafkafi U. (editors) Marcel Dekker Inc., New York, 2002, andreference therein).

For example, a salinity tolerance test can be performed by irrigatingplants at different developmental stages with increasing concentrationsof sodium chloride (for example 50 mM, 100 mM, 200 mM, 400 mM NaCl)applied from the bottom and from above to ensure even dispersal of salt.Following exposure to the stress condition the plants are frequentlymonitored until substantial physiological and/or morphological effectsappear in wild type plants. Thus, the external phenotypic appearance,degree of wilting and overall success to reach maturity and yieldprogeny are compared between control and transgenic plants.

Quantitative parameters of tolerance measured include, but are notlimited to, the average wet and dry weight, growth rate, leaf size, leafcoverage (overall leaf area), the weight of the seeds yielded, theaverage seed size and the number of seeds produced per plant.Transformed plants not exhibiting substantial physiological and/ormorphological effects, or exhibiting higher biomass than wild-typeplants, are identified as abiotic stress tolerant plants.

Osmotic Tolerance Test—

Osmotic stress assays (including sodium chloride and mannitol assays)are conducted to determine if an osmotic stress phenotype was sodiumchloride-specific or if it was a general osmotic stress relatedphenotype. Plants which are tolerant to osmotic stress may have moretolerance to drought and/or freezing. For salt and osmotic stressgermination experiments, the medium is supplemented for example with 50mM, 100 mM, 200 mM NaCl or 100 mM, 200 mM NaCl, 400 mM mannitol.

Drought Tolerance Assay/Osmoticum Assay—

Tolerance to drought is performed to identify the genes conferringbetter plant survival after acute water deprivation. To analyze whetherthe transgenic plants are more tolerant to drought, an osmotic stressproduced by the non-ionic osmolyte sorbitol in the medium can beperformed. Control and transgenic plants are germinated and grown inplant-agar plates for 4 days, after which they are transferred to platescontaining 500 mM sorbitol. The treatment causes growth retardation,then both control and transgenic plants are compared, by measuring plantweight (wet and dry), yield, and by growth rates measured as time toflowering.

Conversely, soil-based drought screens are performed with plantsoverexpressing the polynucleotides detailed above. Seeds from controlArabidopsis plants, or other transgenic plants overexpressing thepolypeptide of the invention are germinated and transferred to pots.Drought stress is obtained after irrigation is ceased accompanied byplacing the pots on absorbent paper to enhance the soil-drying rate.Transgenic and control plants are compared to each other when themajority of the control plants develop severe wilting. Plants arere-watered after obtaining a significant fraction of the control plantsdisplaying a severe wilting. Plants are ranked comparing to controls foreach of two criteria: tolerance to the drought conditions and recovery(survival) following re-watering.

Cold Stress Tolerance—

To analyze cold stress, mature (25 day old) plants are transferred to 4°C. chambers for 1 or 2 weeks, with constitutive light. Later on plantsare moved back to greenhouse. Two weeks later damages from chillingperiod, resulting in growth retardation and other phenotypes, arecompared between both control and transgenic plants, by measuring plantweight (wet and dry), and by comparing growth rates measured as time toflowering, plant size, yield, and the like.

Heat Stress Tolerance—

Heat stress tolerance is achieved by exposing the plants to temperaturesabove 34° C. for a certain period. Plant tolerance is examined aftertransferring the plants back to 22° C. for recovery and evaluation after5 days relative to internal controls (non-transgenic plants) or plantsnot exposed to neither cold or heat stress.

Water Use Efficiency—

can be determined as the biomass produced per unit transpiration. Toanalyze WUE, leaf relative water content can be measured in control andtransgenic plants. Fresh weight (FW) is immediately recorded; thenleaves are soaked for 8 hours in distilled water at room temperature inthe dark, and the turgid weight (TW) is recorded. Total dry weight (DW)is recorded after drying the leaves at 60° C. to a constant weight.Relative water content (RWC) is calculated according to the followingFormula I:

RWC=[(FW−DW)/(TW−DW)]×100  Formula I

Fertilizer Use Efficiency—

To analyze whether the transgenic plants are more responsive tofertilizers, plants are grown in agar plates or pots with a limitedamount of fertilizer, as described, for example, in Yanagisawa et al(Proc Natl Acad Sci USA. 2004; 101:7833-8). The plants are analyzed fortheir overall size, time to flowering, yield, protein content of shootand/or grain. The parameters checked are the overall size of the matureplant, its wet and dry weight, the weight of the seeds yielded, theaverage seed size and the number of seeds produced per plant. Otherparameters that may be tested are: the chlorophyll content of leaves (asnitrogen plant status and the degree of leaf verdure is highlycorrelated), amino acid and the total protein content of the seeds orother plant parts such as leaves or shoots, oil content, etc. Similarly,instead of providing nitrogen at limiting amounts, phosphate orpotassium can be added at increasing concentrations. Again, the sameparameters measured are the same as listed above. In this way, nitrogenuse efficiency (NUE), phosphate use efficiency (PUE) and potassium useefficiency (KUE) are assessed, checking the ability of the transgenicplants to thrive under nutrient restraining conditions.

Nitrogen Use Efficiency—

To analyze whether the transgenic plants (e.g., Arabidopsis plants) aremore responsive to nitrogen, plant are grown in 0.75-3 mM (nitrogendeficient conditions) or 6-10 mM (optimal nitrogen concentration).Plants are allowed to grow for additional 25 days or until seedproduction. The plants are then analyzed for their overall size, time toflowering, yield, protein content of shoot and/or grain/seed production.The parameters checked can be the overall size of the plant, wet and dryweight, the weight of the seeds yielded, the average seed size and thenumber of seeds produced per plant. Other parameters that may be testedare: the chlorophyll content of leaves (as nitrogen plant status and thedegree of leaf greenness is highly correlated), amino acid and the totalprotein content of the seeds or other plant parts such as leaves orshoots and oil content. Transformed plants not exhibiting substantialphysiological and/or morphological effects, or exhibiting highermeasured parameters levels than wild-type plants, are identified asnitrogen use efficient plants.

Nitrogen Use Efficiency Assay Using Plantlets—

The assay is done according to Yanagisawa-S. et al. with minormodifications (“Metabolic engineering with Dofl transcription factor inplants: Improved nitrogen assimilation and growth under low-nitrogenconditions” Proc. Nall. Acad. Sci. USA 101, 7833-7838). Briefly,transgenic plants which are grown for 7-10 days in 0.5×MS[Murashige-Skoog] supplemented with a selection agent are transferred totwo nitrogen-limiting conditions: MS media in which the combinednitrogen concentration (NH₄NO₃ and KNO₃) was 0.75 mM (nitrogen deficientconditions) or 6-15 mM (optimal nitrogen concentration). Plants areallowed to grow for additional 30-40 days and then photographed,individually removed from the Agar (the shoot without the roots) andimmediately weighed (fresh weight) for later statistical analysis.Constructs for which only T1 seeds are available are sown on selectivemedia and at least 20 seedlings (each one representing an independenttransformation event) are carefully transferred to the nitrogen-limitingmedia. For constructs for which T2 seeds are available, differenttransformation events are analyzed. Usually, 20 randomly selected plantsfrom each event are transferred to the nitrogen-limiting media allowedto grow for 3-4 additional weeks and individually weighed at the end ofthat period. Transgenic plants are compared to control plants grown inparallel under the same conditions. Mock-transgenic plants expressingthe uidA reporter gene (GUS) under the same promoter or transgenicplants carrying the same promoter but lacking a reporter gene are usedas control.

Nitrogen Determination—

The procedure for N (nitrogen) concentration determination in thestructural parts of the plants involves the potassium persulfatedigestion method to convert organic N to NO₃ ⁻ (Purcell and King 1996Argon. J. 88:111-113, the modified Cd⁻ mediated reduction of NO₃ ⁻ toNO₂ ⁻ (Vodovotz 1996 Biotechniques 20:390-394) and the measurement ofnitrite by the Griess assay (Vodovotz 1996, supra). The absorbancevalues are measured at 550 nm against a standard curve of NaNO₂. Theprocedure is described in details in Samonte et al. 2006 Agron. J.98:168-176.

Germination Tests—

Germination tests compare the percentage of seeds from transgenic plantsthat could complete the germination process to the percentage of seedsfrom control plants that are treated in the same manner. Normalconditions are considered for example, incubations at 22° C. under22-hour light 2-hour dark daily cycles. Evaluation of germination andseedling vigor is conducted between 4 and 14 days after planting. Thebasal media is 50% MS medium (Murashige and Skoog, 1962 Plant Physiology15, 473-497).

Germination is checked also at unfavorable conditions such as cold(incubating at temperatures lower than 10° C. instead of 22° C.) orusing seed inhibition solutions that contain high concentrations of anosmolyte such as sorbitol (at concentrations of 50 mM, 100 mM, 200 mM,300 mM, 500 mM, and up to 1000 mM) or applying increasing concentrationsof salt (of 50 mM, 100 mM, 200 mM, 300 mM, 500 mM NaCl).

The effect of the transgene on plant's vigor, growth rate, biomass,yield and/or oil content can be determined using known methods.

Plant Vigor—

The plant vigor can be calculated by the increase in growth parameterssuch as leaf area, fiber length, rosette diameter, plant fresh weightand the like per time.

Growth Rate—

The growth rate can be measured using digital analysis of growingplants. For example, images of plants growing in greenhouse on plotbasis can be captured every 3 days and the rosette area can becalculated by digital analysis. Rosette area growth is calculated usingthe difference of rosette area between days of sampling divided by thedifference in days between samples.

Evaluation of growth rate can be done by measuring plant biomassproduced, rosette area, leaf size or root length per time (can bemeasured in cm² per day of leaf area).

Relative growth rate area can be calculated using Formula II.

Relative growth rate area=Regression coefficient of area along timecourse.  Formula II:

Thus, the relative growth area rate is in units of 1/day and lengthgrowth rate is in units of 1/day.

Seed Yield—

Evaluation of the seed yield per plant can be done by measuring theamount (weight or size) or quantity (i.e., number) of dry seeds producedand harvested from 8-16 plants and divided by the number of plants.

For example, the total seeds from 8-16 plants can be collected, weightedusing e.g., an analytical balance and the total weight can be divided bythe number of plants. Seed yield per growing area can be calculated inthe same manner while taking into account the growing area given to asingle plant. Increase seed yield per growing area could be achieved byincreasing seed yield per plant, and/or by increasing number of plantscapable of growing in a given area.

In addition, seed yield can be determined via the weight of 1000 seeds.The weight of 1000 seeds can be determined as follows: seeds arescattered on a glass tray and a picture is taken. Each sample isweighted and then using the digital analysis, the number of seeds ineach sample is calculated.

The 1000 seeds weight can be calculated using formula III:

1000 Seed Weight=number of seed in sample/sample weight×1000  FormulaIII:

The Harvest Index can be calculated using Formula IV

Harvest Index=Average seed yield per plant/Average dry weight  FormulaIV:

Grain Protein Concentration—

Grain protein content (g grain protein m⁻²) is estimated as the productof the mass of grain N (g grain N m⁻²) multiplied by the N/proteinconversion ratio of k-5.13 (Mosse 1990, supra). The grain proteinconcentration is estimated as the ratio of grain protein content perunit mass of the grain (g grain protein kg⁻¹ grain).

Fiber Length—

Fiber length can be measured using fibrograph. The fibrograph system wasused to compute length in terms of “Upper Half Mean” length. The upperhalf mean (UHM) is the average length of longer half of the fiberdistribution. The fibrograph measures length in span lengths at a givenpercentage point (Hypertext Transfer Protocol://World Wide Web (dot)cottoninc (dot) com/ClassificationofCotton/?Pg=4#Length).

According to some embodiments of the invention, increased yield of cornmay be manifested as one or more of the following: increase in thenumber of plants per growing area, increase in the number of ears perplant, increase in the number of rows per ear, number of kernels per earrow, kernel weight, thousand kernel weight (1000-weight), earlength/diameter, increase oil content per kernel and increase starchcontent per kernel.

As mentioned, the increase of plant yield can be determined by variousparameters. For example, increased yield of rice may be manifested by anincrease in one or more of the following: number of plants per growingarea, number of panicles per plant, number of spikelets per panicle,number of flowers per panicle, increase in the seed filling rate,increase in thousand kernel weight (1000-weight), increase oil contentper seed, increase starch content per seed, among others. An increase inyield may also result in modified architecture, or may occur because ofmodified architecture.

Similarly, increased yield of soybean may be manifested by an increasein one or more of the following: number of plants per growing area,number of pods per plant, number of seeds per pod, increase in the seedfilling rate, increase in thousand seed weight (1000-weight), reduce podshattering, increase oil content per seed, increase protein content perseed, among others. An increase in yield may also result in modifiedarchitecture, or may occur because of modified architecture.

Increased yield of canola may be manifested by an increase in one ormore of the following: number of plants per growing area, number of podsper plant, number of seeds per pod, increase in the seed filling rate,increase in thousand seed weight (1000-weight), reduce pod shattering,increase oil content per seed, among others. An increase in yield mayalso result in modified architecture, or may occur because of modifiedarchitecture.

Increased yield of cotton may be manifested by an increase in one ormore of the following: number of plants per growing area, number ofbolls per plant, number of seeds per boll, increase in the seed fillingrate, increase in thousand seed weight (1000-weight), increase oilcontent per seed, improve fiber length, fiber strength, among others. Anincrease in yield may also result in modified architecture, or may occurbecause of modified architecture.

Oil Content—

The oil content of a plant can be determined by extraction of the oilfrom the seed or the vegetative portion of the plant. Briefly, lipids(oil) can be removed from the plant (e.g., seed) by grinding the planttissue in the presence of specific solvents (e.g., hexane or petroleumether) and extracting the oil in a continuous extractor. Indirect oilcontent analysis can be carried out using various known methods such asNuclear Magnetic Resonance (NMR) Spectroscopy, which measures theresonance energy absorbed by hydrogen atoms in the liquid state of thesample [See for example, Conway T F. and Earle F R., 1963, Journal ofthe American Oil Chemists' Society; Springer Berlin/Heidelberg, ISSN:0003-021X (Print) 1558-9331 (Online)]; the Near Infrared (NI)Spectroscopy, which utilizes the absorption of near infrared energy(1100-2500 nm) by the sample; and a method described in WO/2001/023884,which is based on extracting oil a solvent, evaporating the solvent in agas stream which forms oil particles, and directing a light into the gasstream and oil particles which forms a detectable reflected light.

Thus, the present invention is of high agricultural value for promotingthe yield of commercially desired crops (e.g., biomass of vegetativeorgan such as poplar wood, or reproductive organ such as number of seedsor seed biomass).

Any of the transgenic plants described hereinabove or parts thereof maybe processed to produce a feed, meal, protein or oil preparation, suchas for ruminant animals.

The transgenic plants described hereinabove, which exhibit an increasedoil content can be used to produce plant oil (by extracting the oil fromthe plant).

The plant oil (including the seed oil and/or the vegetative portion oil)produced according to the method of the invention may be combined with avariety of other ingredients. The specific ingredients included in aproduct are determined according to the intended use. Exemplary productsinclude animal feed, raw material for chemical modification,biodegradable plastic, blended food product, edible oil, biofuel,cooking oil, lubricant, biodiesel, snack food, cosmetics, andfermentation process raw material. Exemplary products to be incorporatedto the plant oil include animal feeds, human food products such asextruded snack foods, breads, as a food binding agent, aquaculturefeeds, fermentable mixtures, food supplements, sport drinks, nutritionalfood bars, multi-vitamin supplements, diet drinks, and cereal foods.

According to some embodiments of the invention, the oil comprises a seedoil.

According to some embodiments of the invention, the oil comprises avegetative portion oil.

According to some embodiments of the invention, the plant cell forms apart of a plant.

As used herein the term “about” refers to ±10% The terms “comprises”,“comprising”, “includes”, “including”, “having” and their conjugatesmean “including but not limited to”.

The term “consisting of” means “including and limited to”.

The term “consisting essentially of” means that the composition, methodor structure may include additional ingredients, steps and/or parts, butonly if the additional ingredients, steps and/or parts do not materiallyalter the basic and novel characteristics of the claimed composition,method or structure.

As used herein, the singular form “a”, “an” and “the” include pluralreferences unless the context clearly dictates otherwise. For example,the term “a compound” or “at least one compound” may include a pluralityof compounds, including mixtures thereof.

Throughout this application, various embodiments of this invention maybe presented in a range format. It should be understood that thedescription in range format is merely for convenience and brevity andshould not be construed as an inflexible limitation on the scope of theinvention. Accordingly, the description of a range should be consideredto have specifically disclosed all the possible subranges as well asindividual numerical values within that range. For example, descriptionof a range such as from 1 to 6 should be considered to have specificallydisclosed subranges such as from 1 to 3, from 1 to 4, from 1 to 5, from2 to 4, from 2 to 6, from 3 to 6 etc., as well as individual numberswithin that range, for example, 1, 2, 3, 4, 5, and 6. This appliesregardless of the breadth of the range.

Whenever a numerical range is indicated herein, it is meant to includeany cited numeral (fractional or integral) within the indicated range.The phrases “ranging/ranges between” a first indicate number and asecond indicate number and “ranging/ranges from” a first indicate number“to” a second indicate number are used herein interchangeably and aremeant to include the first and second indicated numbers and all thefractional and integral numerals therebetween.

As used herein the term “method” refers to manners, means, techniquesand procedures for accomplishing a given task including, but not limitedto, those manners, means, techniques and procedures either known to, orreadily developed from known manners, means, techniques and proceduresby practitioners of the chemical, pharmacological, biological,biochemical and medical arts.

It is appreciated that certain features of the invention, which are, forclarity, described in the context of separate embodiments, may also beprovided in combination in a single embodiment. Conversely, variousfeatures of the invention, which are, for brevity, described in thecontext of a single embodiment, may also be provided separately or inany suitable subcombination or as suitable in any other describedembodiment of the invention. Certain features described in the contextof various embodiments are not to be considered essential features ofthose embodiments, unless the embodiment is inoperative without thoseelements.

Various embodiments and aspects of the present invention as delineatedhereinabove and as claimed in the claims section below find experimentalsupport in the following examples.

EXAMPLES

Reference is now made to the following examples, which together with theabove descriptions illustrate some embodiments of the invention in a nonlimiting fashion.

Generally, the nomenclature used herein and the laboratory proceduresutilized in the present invention include molecular, biochemical,microbiological and recombinant DNA techniques. Such techniques arethoroughly explained in the literature. See, for example, “MolecularCloning: A laboratory Manual” Sambrook et al., (1989); “CurrentProtocols in Molecular Biology” Volumes I-III Ausubel, R. M., ed.(1994); Ausubel et al., “Current Protocols in Molecular Biology”, JohnWiley and Sons, Baltimore, Md. (1989); Perbal, “A Practical Guide toMolecular Cloning”, John Wiley & Sons, New York (1988); Watson et al.,“Recombinant DNA”, Scientific American Books, New York; Birren et al.(eds) “Genome Analysis: A Laboratory Manual Series”, Vols. 1-4, ColdSpring Harbor Laboratory Press, New York (1998); methodologies as setforth in U.S. Pat. Nos. 4,666,828; 4,683,202; 4,801,531; 5,192,659 and5,272,057; “Cell Biology: A Laboratory Handbook”, Volumes I-III Cellis,J. E., ed. (1994); “Current Protocols in Immunology” Volumes I-IIIColigan J. E., ed. (1994); Stites et al. (eds), “Basic and ClinicalImmunology” (8th Edition), Appleton & Lange, Norwalk, Conn. (1994);Mishell and Shiigi (eds), “Selected Methods in Cellular Immunology”, W.H. Freeman and Co., New York (1980); available immunoassays areextensively described in the patent and scientific literature, see, forexample, U.S. Pat. Nos. 3,791,932; 3,839,153; 3,850,752; 3,850,578;3,853,987; 3,867,517; 3,879,262; 3,901,654; 3,935,074; 3,984,533;3,996,345; 4,034,074; 4,098,876; 4,879,219; 5,011,771 and 5,281,521;“Oligonucleotide Synthesis” Gait, M. J., ed. (1984); “Nucleic AcidHybridization” Hames, B. D., and Higgins S. J., eds. (1985);“Transcription and Translation” Hames, B. D., and Higgins S. J., Eds.(1984); “Animal Cell Culture” Freshney, R. I., ed. (1986); “ImmobilizedCells and Enzymes” IRL Press, (1986); “A Practical Guide to MolecularCloning” Perbal, B., (1984) and “Methods in Enzymology” Vol. 1-317,Academic Press; “PCR Protocols: A Guide To Methods And Applications”,Academic Press, San Diego, Calif. (1990); Marshak et al., “Strategiesfor Protein Purification and Characterization—A Laboratory CourseManual” CSHL Press (1996); all of which are incorporated by reference asif fully set forth herein. Other general references are providedthroughout this document. The procedures therein are believed to be wellknown in the art and are provided for the convenience of the reader. Allthe information contained therein is incorporated herein by reference.

GENERAL EXPERIMENTAL AND BIOINFORMATICS METHODS

RNA Extraction—

Tissues growing at various growth conditions (as described below) weresampled and RNA was extracted using TRIzol Reagent from Invitrogen[Hypertext Transfer Protocol://World Wide Web (dot) invitrogen (dot)com/content (dot)cfm?pageid=469]. Approximately 30-50 mg of tissue wastaken from samples. The weighed tissues were ground using pestle andmortar in liquid nitrogen and resuspended in 500 μl of TRIzol Reagent.To the homogenized lysate, 100 μl of chloroform was added followed byprecipitation using isopropanol and two washes with 75% ethanol. The RNAwas eluted in 30 μl of RNase-free water. RNA samples were cleaned upusing Qiagen's RNeasy® minikit clean-up protocol as per themanufacturer's protocol (QIAGEN Inc, CA USA). For convenience, eachmicro-array expression information tissue type has received anexpression Set ID.

Correlation Analysis—

was performed for selected genes according to some embodiments of theinvention, in which the characterized parameters (measured parametersaccording to the correlation IDs) were used as “x axis” for correlationwith the tissue transcriptome which was used as the “Y axis”. For eachgene and measured parameter a correlation coefficient “R” was calculated(using Pearson correlation) along with a p-value for the significance ofthe correlation. When the correlation coefficient (R) between the levelsof a gene's expression in a certain tissue and a phenotypic performanceacross ecotypes/variety/hybrid is high in absolute value (between0.5-1), there is an association between the gene (specifically theexpression level of this gene) the phenotypic characteristic (e.g.,improved yield, growth rate, nitrogen use efficiency, abiotic stresstolerance and the like).

Example 1 Identifying Genes which Improve Yield and AgronomicalImportant Traits in Plants

The present inventors have identified polynucleotides which expressionthereof in plants can increase yield, fiber yield, fiber quality, growthrate, vigor, biomass, growth rate, oil content, abiotic stress tolerance(ABST), fertilizer use efficiency (FUE) such as nitrogen use efficiency(NUE), and water use efficiency (WUE) of a plant, as follows.

All nucleotide sequence datasets used here were originated from publiclyavailable databases or from performing sequencing using the Solexatechnology (e.g. Barley and Sorghum). Sequence data from 100 differentplant species was introduced into a single, comprehensive database.Other information on gene expression, protein annotation, enzymes andpathways were also incorporated.

Major databases used include:

Genomes

-   -   Arabidopsis genome [TAIR genome version 6 (Hypertext Transfer        Protocol://World Wide Web (dot) arabidopsis (dot) org/)]    -   Rice genome [IRGSP build 4.0 (Hypertext Transfer Protocol://rgp        (dot) dna (dot) affrc (dot) go (dot) jp/IRGSP/)].    -   Poplar [Populus trichocarpa release 1.1 from JGI (assembly        release v1.0) (Hypertext Transfer Protocol://World Wide Web        (dot) genome (dot) jgi-psf (dot) org/)]    -   Brachypodium [JGI 4× assembly, Hypertext Transfer        Protocol://World Wide Web (dot) brachpodium (dot) org)]    -   Soybean [DOE-JGI SCP, version GlymaO (Hypertext Transfer        Protocol://World Wide Web (dot) phytozome (dot) net/)]    -   Grape [French-Italian Public Consortium for Grapevine Genome        Characterization grapevine genome (Hypertext Transfer        Protocol://World Wide Web (dot) genoscope (dot) cns (dot) fr /)]    -   Castobean [TIGR/J Craig Venter Institute 4× assembly [(Hypertext        Transfer Protocol://msc (dot) jcvi (dot) org/r communis]    -   Sorghum [DOE-JGI SCP, version Sbi1 [Hypertext Transfer        Protocol://World Wide Web (dot) phytozome (dot) net/)].    -   Partially assembled genome of Maize [Hypertext Transfer        Protocol://maizesequence (dot) org/]

Expressed EST and mRNA Sequences were Extracted from the FollowingDatabases:

-   -   GenBank Hypertext Transfer Protocol://World Wide Web (dot) ncbi        (dot) nlm (dot) nih (dot) gov/dbEST    -   RefSeq (Hypertext Transfer Protocol://World Wide Web (dot) ncbi        (dot) nlm (dot) nih (dot) gov/RefSeq/).    -   TAR (Hypertext Transfer Protocol://World Wide Web (dot)        arabidopsis (dot) org/).

Protein and Pathway Databases

-   -   Uniprot [Hypertext Transfer Protocol://World Wide Web (dot)        uniprot (dot) orga    -   AraCyc [Hypertext Transfer Protocol://World Wide Web (dot)        arabidopsis (dot) org/biocyc/index (dot) jsp].    -   ENZYME [Hypertext Transfer Protocol://expasy (dot) org/enzyme/].

Microarray Datasets were Downloaded from:

-   -   GEO (Hypertext Transfer Protocol://World Wide        Web.ncbi.nlm.nih.gov/geo/)    -   TAR (Hypertext Transfer Protocol://World Wide Web.        arabidopsis.org/).    -   Proprietary microarray data (WO2008/122980).

QTL and SNPs Information

-   -   Gramene [Hypertext Transfer Protocol://World Wide Web (dot)        gramene (dot) org/qtl/].    -   Panzea [Hypertext Transfer Protocol://World Wide Web (dot)        panzea (dot) org/index (dot) html].

Database Assembly—

was performed to build a wide, rich, reliable annotated and easy toanalyze database comprised of publicly available genomic mRNA, ESTs DNAsequences, data from various crops as well as gene expression, proteinannotation and pathway data QTLs, and other relevant information.

Database assembly is comprised of a toolbox of gene refining,structuring, annotation and analysis tools enabling to construct atailored database for each gene discovery project. Gene refining andstructuring tools enable to reliably detect splice variants andantisense transcripts, generating understanding of various potentialphenotypic outcomes of a single gene. The capabilities of the “LEADS”platform of Compugen LTD for analyzing human genome have been confirmedand accepted by the scientific community [see e.g., “WidespreadAntisense Transcription”, Yelin, et al. (2003) Nature Biotechnology 21,379-85; “Splicing of Alu Sequences”, Lev-Maor, et al. (2003) Science 300(5623), 1288-91; “Computational analysis of alternative splicing usingEST tissue information”, Xie H et al. Genomics 2002], and have beenproven most efficient in plant genomics as well.

EST Clustering and Gene Assembly—

For gene clustering and assembly of organisms with available genomesequence data (arabidopsis, rice, castorbean, grape, brachypodium,poplar, soybean, sorghum) the genomic LEADS version (GANG) was employed.This tool allows most accurate clustering of ESTs and mRNA sequences ongenome, and predicts gene structure as well as alternative splicingevents and anti-sense transcription.

For organisms with no available full genome sequence data, “expressedLEADS” clustering software was applied.

Gene Annotation—

Predicted genes and proteins were annotated as follows:

Blast search [Hypertext Transfer Protocol://blast (dot) ncbi (dot) nlm(dot) nih (dot) gov /Blast (dot) cgi] against all plant UniProt[Hypertext Transfer Protocol://World Wide Web (dot) uniprot (dot) org/]sequences was performed. Open reading frames of each putative transcriptwere analyzed and longest ORF with higher number of homologues wasselected as predicted protein of the transcript. The predicted proteinswere analyzed by InterPro [Hypertext Transfer Protocol://World Wide Web(dot) ebi (dot) ac (dot) uk/interpro/].

Blast against proteins from AraCyc and ENZYME databases was used to mapthe predicted transcripts to AraCyc pathways.

Predicted proteins from different species were compared using blastalgorithm [Hypertext Transfer Protocol://World Wide Web (dot) ncbi (dot)nlm (dot) nih (dot) gov /Blast (dot) cgi] to validate the accuracy ofthe predicted protein sequence, and for efficient detection oforthologs.

Gene Expression Profiling—

Several data sources were exploited for gene expression profiling,namely microarray data and digital expression profile (see below).According to gene expression profile, a correlation analysis wasperformed to identify genes which are co-regulated under differentdevelopment stages and environmental conditions and associated withdifferent phenotypes.

Publicly available microarray datasets were downloaded from TAR and NCBIGEO sites, renormalized, and integrated into the database. Expressionprofiling is one of the most important resource data for identifyinggenes important for yield.

A digital expression profile summary was compiled for each clusteraccording to all keywords included in the sequence records comprisingthe cluster. Digital expression, also known as electronic Northern Blot,is a tool that displays virtual expression profile based on the ESTsequences forming the gene cluster. The tool provides the expressionprofile of a cluster in terms of plant anatomy (e.g., the tissue/organin which the gene is expressed), developmental stage (the developmentalstages at which a gene can be found) and profile of treatment (providesthe physiological conditions under which a gene is expressed such asdrought, cold, pathogen infection, etc). Given a random distribution ofESTs in the different clusters, the digital expression provides aprobability value that describes the probability of a cluster having atotal of N ESTs to contain X ESTs from a certain collection oflibraries. For the probability calculations, the following is taken intoconsideration: a) the number of ESTs in the cluster, b) the number ofESTs of the implicated and related libraries, c) the overall number ofESTs available representing the species. Thereby clusters with lowprobability values are highly enriched with ESTs from the group oflibraries of interest indicating a specialized expression.

Recently, the accuracy of this system was demonstrated by Portnoy etal., 2009 (Analysis Of The Melon Fruit Transcriptome Based On 454Pyrosequencing) in: Plant & Animal Genomes XVII Conference, San Diego,Calif. Transcriptomic analysis, based on relative EST abundance in datawas performed by 454 pyrosequencing of cDNA representing mRNA of themelon fruit. Fourteen double strand cDNA samples obtained from twogenotypes, two fruit tissues (flesh and rind) and four developmentalstages were sequenced. GS FLX pyrosequencing (Roche/454 Life Sciences)of non-normalized and purified cDNA samples yielded 1,150,657 expressedsequence tags, that assembled into 67,477 unigenes (32,357 singletonsand 35,120 contigs). Analysis of the data obtained against the CucurbitGenomics Database [Hypertext Transfer Protocol://World Wide Web (dot)icugi (dot) org/[confirmed the accuracy of the sequencing and assembly.Expression patterns of selected genes fitted well their qRT-PCR data.

Overall, 213 genes (SEQ ID NOs: 1-288 and 289-480 for polynucleotidesand SEQ ID NOs: 481-727 and 728-792 for polypeptides) were identified tohave a major impact on plant yield, growth rate, vigor, biomass, growthrate, oil content, abiotic stress tolerance, nitrogen use efficiency,water use efficiency and fertilizer use efficiency when expressionthereof is increased in plants. The identified genes, their curatedpolynucleotide and polypeptide sequences, as well as their updatedsequences according to Genbank database are summarized in Table 1,hereinbelow.

TABLE 1 Identified genes for increasing yield, growth rate, vigor,biomass, growth rate, oil content, abiotic stress tolerance, nitrogenuse efficiency, water use efficiency and fertilizer use efficiency of aplant Gene Polyn. SEQ Polyp. SEQ Name Cluster Name Organism ID NO: IDNO: LYM46 barley|gb157SOLEXA|AV914235 barley 1 481 LYM297arabidopsis|gb165|AT2G36560 arabidopsis 2 482 LYM298arabidopsis|gb165|AT3G04550 arabidopsis 3 483 LYM299arabidopsis|gb165|AT5G45360 arabidopsis 4 484 LYM300barley|gb157SOLEXA|AF039024 barley 5 485 LYM301barley|gb157SOLEXA|AJ471689 barley 6 486 LYM302barley|gb157SOLEXA|AJ478368 barley 7 487 LYM303barley|gb157SOLEXA|AL450771 barley 8 488 LYM304barley|gb157SOLEXA|AL500954 barley 9 489 LYM305barley|gb157SOLEXA|AL501188 barley 10 490 LYM306barley|gb157SOLEXA|AL507201 barley 11 491 LYM307barley|gb157SOLEXA|AV832846 barley 12 492 LYM308barley|gb157SOLEXA|AV833964 barley 13 493 LYM309barley|gb157SOLEXA|AV834630 barley 14 494 LYM310barley|gb157SOLEXA|AV836092 barley 15 495 LYM312barley|gb157SOLEXA|AV932936 barley 16 496 LYM313barley|gb157SOLEXA|BE060106 barley 17 497 LYM314barley|gb157SOLEXA|BE412725 barley 18 498 LYM315barley|gb157SOLEXA|BE412988 barley 19 499 LYM316barley|gb157SOLEXA|BE412990 barley 20 500 LYM317barley|gb157SOLEXA|BE413214 barley 21 501 LYM318barley|gb157SOLEXA|BE413493 barley 22 502 LYM319barley|gb157SOLEXA|BE421137 barley 23 503 LYM320barley|gb157SOLEXA|BE421502 barley 24 504 LYM321barley|gb157SOLEXA|BE437947 barley 25 505 LYM322barley|gb157SOLEXA|BE438129 barley 26 506 LYM323barley|gb157SOLEXA|BF263342 barley 27 507 LYM324barley|gb157SOLEXA|BF264152 barley 28 508 LYM326barley|gb157SOLEXA|BF623943 barley 29 509 LYM327barley|gb157SOLEXA|BF628395 barley 30 510 LYM328barley|gb157SOLEXA|BG299354 barley 31 511 LYM329barley|gb157SOLEXA|BG300782 barley 32 512 LYM330barley|gb157SOLEXA|BG366539 barley 33 513 LYM331barley|gb157SOLEXA|BG415251 barley 34 514 LYM332barley|gb157SOLEXA|BI947101 barley 35 515 LYM333barley|gb157SOLEXA|BI951290 barley 36 516 LYM334barley|gb157SOLEXA|BI953288 barley 37 517 LYM335barley|gb157SOLEXA|BJ447518 barley 38 518 LYM336barley|gb157SOLEXA|BQ665724 barley 39 519 LYM338barley|gb157SOLEXA|BU977002 barley 40 520 LYM339barley|gb157SOLEXA|CB875456 barley 41 521 LYM340brachypodium|09v1|GT776162 brachypodium 42 522 LYM341brachypodium|09v1|SRR031795S0011089 brachypodium 43 523 LYM342brachypodium|09v1|SRR031795S0018843 brachypodium 44 524 LYM343cotton|gb164|AW186747 cotton 45 525 LYM344 cotton|gb164|AW187142 cotton46 526 LYM345 cotton|gb164|CO128772 cotton 47 527 LYM346maize|gb170|AA979954 maize 48 528 LYM348 maize|gb170|AI491658 maize 49529 LYM349 maize|gb170|AI586701 maize 50 530 LYM350 maize|gb170|AI612450maize 51 531 LYM351 maize|gb170|AI629497 maize 52 532 LYM352maize|gb170|AI649898 maize 53 533 LYM353 maize|gb170|AI714592 maize 54534 LYM354 maize|gb170|AI734481 maize 55 535 LYM355 maize|gb170|AI734524maize 56 536 LYM356 maize|gb170|AI820388 maize 57 537 LYM357maize|gb170|AI834390 maize 58 538 LYM359 maize|gb170|AI939790 maize 59539 LYM360 maize|gb170|AI964644 maize 60 540 LYM361 maize|gb170|AI978097maize 61 541 LYM362 maize|gb170|AW053081 maize 62 542 LYM363maize|gb170|AW053216 maize 63 543 LYM364 maize|gb170|AW066128 maize 64544 LYM365 maize|gb170|AW066984 maize 65 545 LYM366 maize|gb170|AW119986maize 66 546 LYM367 maize|gb170|AW163846 maize 67 547 LYM368maize|gb170|AW267659 maize 68 548 LYM369 maize|gb170|AW400051 maize 69549 LYM370 maize|gb170|AW455701 maize 70 550 LYM371 maize|gb170|AW461159maize 71 551 LYM372 maize|gb170|AW499159 maize 72 552 LYM373maize|gb170|AW573473 maize 73 553 LYM374 maize|gb170|BE238502 maize 74554 LYM375 maize|gb170|BE512179 maize 75 555 LYM376 maize|gb170|BG842270maize 76 556 LYM377 maize|gb170|BI398419 maize 77 557 LYM378maize|gb170|BM075597 maize 78 558 LYM379 maize|gb170|BM953346 maize 79559 LYM380 maize|gb170|BQ294380 maize 80 560 LYM381 maize|gb170|BU197916maize 81 561 LYM382 maize|gb170|CF005206 maize 82 562 LYM383maize|gb170|CK145349 maize 83 563 LYM384 maize|gb170|DR786060 maize 84564 LYM385 maize|gb170|DT942887 maize 85 565 LYM386 maize|gb170|DW783146maize 86 566 LYM387 maize|gb170|T18700 maize 87 567 LYM388maize|gb170|W49854 maize 88 568 LYM389 rice|gb170|GFXAP002539X8 rice 89569 LYM390 rice|gb170|OS01G10070 rice 90 570 LYM391rice|gb170|OS01G13930 rice 91 571 LYM392 rice|gb170|OS01G42870 rice 92572 LYM393 rice|gb170|OS01G45470 rice 93 573 LYM394rice|gb170|OS01G72670 rice 94 574 LYM395 rice|gb170|OS02G03230 rice 95575 LYM396 rice|gb170|OS02G12310 rice 96 576 LYM397rice|gb170|OS02G44510 rice 97 577 LYM398 rice|gb170|OS02G58150 rice 98578 LYM399 rice|gb170|OS03G04470 rice 99 579 LYM400rice|gb170|OS03G14690 rice 100 580 LYM401 rice|gb170|OS03G17490 rice 101581 LYM402 rice|gb170|OS03G53660 rice 102 582 LYM403rice|gb170|OS04G53300 rice 103 583 LYM404 rice|gb170|OS04G54240 rice 104584 LYM405 rice|gb170|OS04G58890 rice 105 585 LYM406rice|gb170|OS04G59050 rice 106 586 LYM407 rice|gb170|OS05G05680 rice 107587 LYM408 rice|gb170|OS05G35340 rice 108 588 LYM409rice|gb170|OS05G42270 rice 109 589 LYM410 rice|gb170|OS06G43760 rice 110590 LYM411 rice|gb170|OS07G10350 rice 111 591 LYM412rice|gb170|OS07G42220 rice 112 592 LYM413 rice|gb170|OS07G42390 rice 113593 LYM414 rice|gb170|OS09G12150 rice 114 594 LYM415rice|gb170|OS09G31120 rice 115 595 LYM416 rice|gb170|OS10G27450 rice 116596 LYM417 rice|gb170|OS10G34920 rice 117 597 LYM418rice|gb170|OS11G08940 rice 118 598 LYM419 sorghum|09v1|AW285700 sorghum119 599 LYM421 sorghum|09v1|AW565098 sorghum 120 600 LYM423sorghum|09v1|BE367258 sorghum 121 601 LYM424 sorghum|09v1|BF507223sorghum 122 602 LYM427 sorghum|09v1|BG463613 sorghum 123 603 LYM433sorghum|09v1|CF481648 sorghum 124 604 LYM435 sorghum|09v1|SB01G001570sorghum 125 605 LYM436 sorghum|09v1|SB01G001880 sorghum 126 606 LYM437sorghum|09v1|SB01G005600 sorghum 127 607 LYM438 sorghum|09v1|SB01G009590sorghum 128 608 LYM439 sorghum|09v1|SB01G012100 sorghum 129 609 LYM440sorghum|09v1|SB01G022260 sorghum 130 610 LYM441 sorghum|09v1|SB01G028160sorghum 131 611 LYM442 sorghum|09v1|SB01G036980 sorghum 132 612 LYM443sorghum|09v1|SB01G038030 sorghum 133 613 LYM444 sorghum|09v1|SB01G041100sorghum 134 614 LYM445 sorghum|09v1|SB01G045170 sorghum 135 615 LYM446sorghum|09v1|SB01G045830 sorghum 136 616 LYM447 sorghum|09v1|SB01G045970sorghum 137 617 LYM448 sorghum|09v1|SB01G047790 sorghum 138 618 LYM449sorghum|09v1|SB01G049680 sorghum 139 619 LYM450 sorghum|09v1|SB02G002380sorghum 140 620 LYM451 sorghum|09v1|SB02G003540 sorghum 141 621 LYM452sorghum|09v1|SB02G005600 sorghum 142 622 LYM453 sorghum|09v1|SB02G024770sorghum 143 623 LYM454 sorghum|09v1|SB02G036860 sorghum 144 624 LYM455sorghum|09v1|SB02G042460 sorghum 145 625 LYM456 sorghum|09v1|SB03G000620sorghum 146 626 LYM457 sorghum|09v1|SB03G002840 sorghum 147 627 LYM458sorghum|09v1|SB03G005490 sorghum 148 628 LYM460 sorghum|09v1|SB03G010610sorghum 149 629 LYM461 sorghum|09v1|SB03G028800 sorghum 150 630 LYM463sorghum|09v1|SB03G036240 sorghum 151 631 LYM464 sorghum|09v1|SB03G037450sorghum 152 632 LYM465 sorghum|09v1|SB03G042320 sorghum 153 633 LYM466sorghum|09v1|SB03G042690 sorghum 154 634 LYM467 sorghum|09v1|SB03G044230sorghum 155 635 LYM468 sorghum|09v1|SB03G046070 sorghum 156 636 LYM471sorghum|09v1|SB04G009670 sorghum 157 637 LYM472 sorghum|09v1|SB04G017800sorghum 158 638 LYM473 sorghum|09v1|SB04G020170 sorghum 159 639 LYM474sorghum|09v1|SB04G022570 sorghum 160 640 LYM475 sorghum|09v1|SB04G023155sorghum 161 641 LYM476 sorghum|09v1|SB04G028950 sorghum 162 642 LYM477sorghum|09v1|SB04G030560 sorghum 163 643 LYM478 sorghum|09v1|SB05G000940sorghum 164 644 LYM479 sorghum|09v1|SB05G000980 sorghum 165 645 LYM480sorghum|09v1|SB05G001550 sorghum 166 646 LYM481 sorghum|09v1|SB05G005450sorghum 167 647 LYM483 sorghum|09v1|SB05G018376 sorghum 168 648 LYM484sorghum|09v1|SB05G019020 sorghum 169 649 LYM485 sorghum|09v1|SB06G021970sorghum 170 650 LYM486 sorghum|09v1|SB06G024300 sorghum 171 651 LYM487sorghum|09v1|SB06G027830 sorghum 172 652 LYM488 sorghum|09v1|SB06G029440sorghum 173 653 LYM489 sorghum|09v1|SB06G030740 sorghum 174 654 LYM490sorghum|09v1|SB06G032170 sorghum 175 655 LYM491 sorghum|09v1|SB06G033090sorghum 176 656 LYM492 sorghum|09v1|SB07G001470 sorghum 177 657 LYM493sorghum|09v1|SB07G003070 sorghum 178 658 LYM494 sorghum|09v1|SB07G005420sorghum 179 659 LYM495 sorghum|09v1|SB07G027350 sorghum 180 660 LYM496sorghum|09v1|SB07G027880 sorghum 181 661 LYM497 sorghum|09v1|SB08G000390sorghum 182 662 LYM498 sorghum|09v1|SB08G000930 sorghum 183 663 LYM499sorghum|09v1|SB08G002960 sorghum 184 664 LYM500 sorghum|09v1|SB08G007640sorghum 185 665 LYM501 sorghum|09v1|SB08G009120 sorghum 186 666 LYM502sorghum|09v1|SB08G019150 sorghum 187 667 LYM503 sorghum|09v1|SB08G019960sorghum 188 668 LYM504 sorghum|09v1|SB08G022310 sorghum 189 669 LYM505sorghum|09v1|SB09G004700 sorghum 190 670 LYM506 sorghum|09v1|SB10G023650sorghum 191 671 LYM507 sorghum|09v1|SB10G023690 sorghum 192 672 LYM508sorghum|09v1|SB10G026350 sorghum 193 673 LYM509 sorghum|09v1|SB10G029550sorghum 194 674 LYM510 wheat|gb164|CA745761 wheat 195 675 LYM304_H3brachypodium|09v1|DV468923 brachypodium 196 676 LYM307_H7sorghum|09v1|SB01G033760 sorghum 197 677 LYM326_H4brachypodium|09v1|GT790559 brachypodium 198 678 LYM368_H4sorghum|09v1|SB04G022750 sorghum 199 679 LYM397_H2sorghum|09v1|SB04G036540 sorghum 200 680 LYM311barley|gb157SOLEXA|AV909117 barley 201 — LYM325barley|gb157SOLEXA|BF623560 barley 202 — LYM337barley|gb157SOLEXA|BQ756072 barley 203 — LYM420 sorghum|09v1|AW287430sorghum 204 — LYM422 sorghum|09v1|AW745990 sorghum 205 — LYM425sorghum|09v1|BF655529 sorghum 206 — LYM426 sorghum|09v1|BG050685 sorghum207 — LYM428 sorghum|09v1|BG947594 sorghum 208 — LYM429sorghum|09v1|BI140081 sorghum 209 — LYM430 sorghum|09v1|CD208778 sorghum210 — LYM431 sorghum|09v1|CD210000 sorghum 211 — LYM432sorghum|09v1|CF073969 sorghum 212 — LYM434 sorghum|09v1|CF758775 sorghum213 — LYM298 arabidopsis|gb165|AT3G04550 arabidopsis 3 683 LYM396rice|gb170|OS02G12310 rice 96 708 LYM409 rice|gb170|OS05G42270 rice 109710 LYM440 sorghum|09v1|SB01G022260 sorghum 130 718 LYM46barley|gb157SOLEXA|AV914235 barley 214 681 LYM297arabidopsis|gb165|AT2G36560 arabidopsis 215 682 LYM305barley|gb157SOLEXA|AL501188 barley 216 684 LYM308barley|gb157SOLEXA|AV833964 barley 217 493 LYM309barley|gb157SOLEXA|AV834630 barley 218 685 LYM312barley|gb157SOLEXA|AV932936 barley 219 686 LYM315barley|gb157SOLEXA|BE412988 barley 220 687 LYM316barley|gb157SOLEXA|BE412990 barley 221 688 LYM323barley|gb157SOLEXA|BF263342 barley 222 689 LYM331barley|gb157SOLEXA|BG415251 barley 223 690 LYM336barley|gb157SOLEXA|BQ665724 barley 224 691 LYM337barley|gb157SOLEXA|BQ756072 barley 225 692 LYM338barley|gb157SOLEXA|BU977002 barley 226 693 LYM339barley|gb157SOLEXA|CB875456 barley 227 694 LYM340brachypodium|09v1|GT776162 brachypodium 228 522 LYM341brachypodium|09v1|SRR031795S0011089 brachypodium 229 523 LYM342brachypodium|09v1|SRR031795S0018843 brachypodium 230 695 LYM345cotton|gb164|CO128772 cotton 231 696 LYM353 maize|gb170|AI714592 maize232 697 LYM356 maize|gb170|AI820388 maize 233 698 LYM357maize|gb170|AI834390 maize 234 699 LYM360 maize|gb170|AI964644 maize 235700 LYM362 maize|gb170|AW053081 maize 236 701 LYM364maize|gb170|AW066128 maize 237 544 LYM365 maize|gb170|AW066984 maize 238545 LYM370 maize|gb170|AW455701 maize 239 702 LYM371maize|gb170|AW461159 maize 240 703 LYM374 maize|gb170|BE238502 maize 241704 LYM378 maize|gb170|BM075597 maize 242 558 LYM381maize|gb170|BU197916 maize 243 705 LYM384 maize|gb170|DR786060 maize 244706 LYM386 maize|gb170|DW783146 maize 245 707 LYM401rice|gb170|OS03G17490 rice 246 581 LYM402 rice|gb170|OS03G53660 rice 247582 LYM408 rice|gb170|OS05G35340 rice 248 709 LYM411rice|gb170|OS07G10350 rice 249 711 LYM414 rice|gb170|OS09G12150 rice 250594 LYM417 rice|gb170|OS10G34920 rice 251 712 LYM421sorghum|09v1|AW565098 sorghum 252 713 LYM424 sorghum|09v1|BF507223sorghum 253 714 LYM427 sorghum|09v1|BG463613 sorghum 254 715 LYM428sorghum|09v1|BG947594 sorghum 255 716 LYM433 sorghum|09v1|CF481648sorghum 256 717 LYM443 sorghum|09v1|SB01G038030 sorghum 257 613 LYM445sorghum|09v1|SB01G045170 sorghum 258 719 LYM446 sorghum|09v1|SB01G045830sorghum 259 616 LYM447 sorghum|09v1|SB01G045970 sorghum 260 617 LYM451sorghum|09v1|SB02G003540 sorghum 261 621 LYM455 sorghum|09v1|SB02G042460sorghum 262 625 LYM457 sorghum|09v1|SB03G002840 sorghum 263 627 LYM460sorghum|09v1|SB03G010610 sorghum 264 720 LYM465 sorghum|09v1|SB03G042320sorghum 265 721 LYM467 sorghum|09v1|SB03G044230 sorghum 266 722 LYM468sorghum|09v1|SB03G046070 sorghum 267 636 LYM472 sorghum|09v1|SB04G017800sorghum 268 723 LYM475 sorghum|09v1|SB04G023155 sorghum 269 724 LYM479sorghum|09v1|SB05G000980 sorghum 270 725 LYM483 sorghum|09v1|SB05G018376sorghum 271 726 LYM484 sorghum|09v1|SB05G019020 sorghum 272 727 LYM488sorghum|09v1|SB06G029440 sorghum 273 653 LYM490 sorghum|09v1|SB06G032170sorghum 274 655 LYM491 sorghum|09v1|SB06G033090 sorghum 275 656 LYM497sorghum|09v1|SB08G000390 sorghum 276 662 LYM502 sorghum|09v1|SB08G019150sorghum 277 667 LYM504 sorghum|09v1|SB08G022310 sorghum 278 669LYM307_H7 sorghum|09v1|SB01G033760 sorghum 279 677 LYM311barley|gb157SOLEXA|AV909117 barley 280 — LYM325barley|gb157SOLEXA|BF623560 barley 281 — LYM420 sorghum|09v1|AW287430sorghum 282 — LYM422 sorghum|09v1|AW745990 sorghum 283 — LYM425sorghum|09v1|BF655529 sorghum 284 — LYM426 sorghum|09v1|BG050685 sorghum285 — LYM429 sorghum|09v1|BI140081 sorghum 286 — LYM432sorghum|09v1|CF073969 sorghum 287 — LYM434 sorghum|09v1|CF758775 sorghum288 — LYM297 arabidopsis|gb165|AT2G36560 arabidopsis 2 482 LYM337barley|gb157SOLEXA|BQ756072 barley 203 — LYM425 sorghum|09v1|BF655529sorghum 206 — LYM428 sorghum|09v1|BG947594 sorghum 208 — LYM434sorghum|09v1|CF758775 sorghum 213 — LYM46 barley|gb157SOLEXA|AV914235barley 289 481 LYM298 arabidopsis|gb165|AT3G04550 arabidopsis 290 483LYM299 arabidopsis|gb165|AT5G45360 arabidopsis 291 484 LYM300barley|gb157SOLEXA|AF039024 barley 292 485 LYM301barley|gb157SOLEXA|AJ471689 barley 293 486 LYM302barley|gb157SOLEXA|AJ478368 barley 294 487 LYM303barley|gb157SOLEXA|AL450771 barley 295 728 LYM305barley|gb157SOLEXA|AL501188 barley 296 729 LYM306barley|gb157SOLEXA|AL507201 barley 297 491 LYM308barley|gb157SOLEXA|AV833964 barley 298 493 LYM309barley|gb157SOLEXA|AV834630 barley 299 494 LYM310barley|gb157SOLEXA|AV836092 barley 300 495 LYM312barley|gb157SOLEXA|AV932936 barley 301 730 LYM313barley|gb157SOLEXA|BE060106 barley 302 497 LYM314barley|gb157SOLEXA|BE412725 barley 303 498 LYM315barley|gb157SOLEXA|BE412988 barley 304 499 LYM316barley|gb157SOLEXA|BE412990 barley 305 500 LYM317barley|gb157SOLEXA|BE413214 barley 306 501 LYM318barley|gb157SOLEXA|BE413493 barley 307 502 LYM319barley|gb157SOLEXA|BE421137 barley 308 503 LYM320barley|gb157SOLEXA|BE421502 barley 309 731 LYM321barley|gb157SOLEXA|BE437947 barley 310 732 LYM322barley|gb157SOLEXA|BE438129 barley 311 733 LYM323barley|gb157SOLEXA|BF263342 barley 312 734 LYM324barley|gb157SOLEXA|BF264152 barley 313 735 LYM327barley|gb157SOLEXA|BF628395 barley 314 736 LYM328barley|gb157SOLEXA|BG299354 barley 315 737 LYM329barley|gb157SOLEXA|BG300782 barley 316 738 LYM330barley|gb157SOLEXA|BG366539 barley 317 739 LYM331barley|gb157SOLEXA|BG415251 barley 318 740 LYM332barley|gb157SOLEXA|BI947101 barley 319 741 LYM333barley|gb157SOLEXA|BI951290 barley 320 516 LYM334barley|gb157SOLEXA|BI953288 barley 321 517 LYM335barley|gb157SOLEXA|BJ447518 barley 322 518 LYM336barley|gb157SOLEXA|BQ665724 barley 323 742 LYM338barley|gb157SOLEXA|BU977002 barley 324 693 LYM339barley|gb157SOLEXA|CB875456 barley 325 743 LYM340brachypodium|09v1|GT776162 brachypodium 326 744 LYM341brachypodium|09v1|SRR031795S0011089 brachypodium 327 523 LYM343cotton|gb164|AW186747 cotton 328 745 LYM344 cotton|gb164|AW187142 cotton329 746 LYM345 cotton|gb164|CO128772 cotton 330 747 LYM346maize|gb170|AA979954 maize 331 748 LYM348 maize|gb170|AI491658 maize 332749 LYM349 maize|gb170|AI586701 maize 333 530 LYM350maize|gb170|AI612450 maize 334 531 LYM351 maize|gb170|AI629497 maize 335532 LYM352 maize|gb170|AI649898 maize 336 533 LYM353maize|gb170|AI714592 maize 337 750 LYM354 maize|gb170|AI734481 maize 338751 LYM355 maize|gb170|AI734524 maize 339 752 LYM356maize|gb170|AI820388 maize 340 537 LYM357 maize|gb170|AI834390 maize 341538 LYM359 maize|gb170|AI939790 maize 342 539 LYM360maize|gb170|AI964644 maize 343 540 LYM361 maize|gb170|AI978097 maize 344541 LYM362 maize|gb170|AW053081 maize 345 542 LYM363maize|gb170|AW053216 maize 346 753 LYM364 maize|gb170|AW066128 maize 347754 LYM365 maize|gb170|AW066984 maize 348 545 LYM366maize|gb170|AW119986 maize 349 755 LYM367 maize|gb170|AW163846 maize 350756 LYM369 maize|gb170|AW400051 maize 351 757 LYM370maize|gb170|AW455701 maize 352 758 LYM371 maize|gb170|AW461159 maize 353759 LYM372 maize|gb170|AW499159 maize 354 760 LYM373maize|gb170|AW573473 maize 355 761 LYM374 maize|gb170|BE238502 maize 356554 LYM375 maize|gb170|BE512179 maize 357 762 LYM376maize|gb170|BG842270 maize 358 556 LYM377 maize|gb170|BI398419 maize 359557 LYM378 maize|gb170|BM075597 maize 360 558 LYM379maize|gb170|BM953346 maize 361 559 LYM380 maize|gb170|BQ294380 maize 362560 LYM381 maize|gb170|BU197916 maize 363 763 LYM382maize|gb170|CF005206 maize 364 764 LYM384 maize|gb170|DR786060 maize 365564 LYM385 maize|gb170|DT942887 maize 366 765 LYM386maize|gb170|DW783146 maize 367 566 LYM387 maize|gb170|T18700 maize 368766 LYM388 maize|gb170|W49854 maize 369 568 LYM389rice|gb170|GFXAP002539X8 rice 370 569 LYM390 rice|gb170|OS01G10070 rice371 570 LYM391 rice|gb170|OS01G13930 rice 372 571 LYM392rice|gb170|OS01G42870 rice 373 572 LYM393 rice|gb170|OS01G45470 rice 374573 LYM394 rice|gb170|OS01G72670 rice 375 574 LYM395rice|gb170|OS02G03230 rice 376 575 LYM396 rice|gb170|OS02G12310 rice 377576 LYM398 rice|gb170|OS02G58150 rice 378 578 LYM399rice|gb170|OS03G04470 rice 379 579 LYM400 rice|gb170|OS03G14690 rice 380580 LYM401 rice|gb170|OS03G17490 rice 381 767 LYM402rice|gb170|OS03G53660 rice 382 582 LYM403 rice|gb170|OS04G53300 rice 383583 LYM404 rice|gb170|OS04G54240 rice 384 584 LYM405rice|gb170|OS04G58890 rice 385 585 LYM406 rice|gb170|OS04G59050 rice 386586 LYM407 rice|gb170|OS05G05680 rice 387 587 LYM409rice|gb170|OS05G42270 rice 388 589 LYM410 rice|gb170|OS06G43760 rice 389768 LYM413 rice|gb170|OS07G42390 rice 390 593 LYM414rice|gb170|OS09G12150 rice 391 769 LYM415 rice|gb170|OS09G31120 rice 392595 LYM416 rice|gb170|OS10G27450 rice 393 596 LYM417rice|gb170|OS10G34920 rice 394 597 LYM418 rice|gb170|OS11G08940 rice 395598 LYM419 sorghum|09v1|AW285700 sorghum 396 599 LYM421sorghum|09v1|AW565098 sorghum 397 600 LYM423 sorghum|09v1|BE367258sorghum 398 601 LYM424 sorghum|09v1|BF507223 sorghum 399 770 LYM427sorghum|09v1|BG463613 sorghum 400 603 LYM433 sorghum|09v1|CF481648sorghum 401 604 LYM435 sorghum|09v1|SB01G001570 sorghum 402 605 LYM436sorghum|09v1|SB01G001880 sorghum 403 606 LYM437 sorghum|09v1|SB01G005600sorghum 404 607 LYM438 sorghum|09v1|SB01G009590 sorghum 405 608 LYM439sorghum|09v1|SB01G012100 sorghum 406 609 LYM440 sorghum|09v1|SB01G022260sorghum 407 610 LYM441 sorghum|09v1|SB01G028160 sorghum 408 771 LYM442sorghum|09v1|SB01G036980 sorghum 409 612 LYM443 sorghum|09v1|SB01G038030sorghum 410 613 LYM444 sorghum|09v1|SB01G041100 sorghum 411 772 LYM445sorghum|09v1|SB01G045170 sorghum 412 773 LYM446 sorghum|09v1|SB01G045830sorghum 413 616 LYM447 sorghum|09v1|SB01G045970 sorghum 414 617 LYM448sorghum|09v1|SB01G047790 sorghum 415 618 LYM449 sorghum|09v1|SB01G049680sorghum 416 619 LYM450 sorghum|09v1|SB02G002380 sorghum 417 620 LYM451sorghum|09v1|SB02G003540 sorghum 418 621 LYM452 sorghum|09v1|SB02G005600sorghum 419 622 LYM453 sorghum|09v1|SB02G024770 sorghum 420 774 LYM454sorghum|09v1|SB02G036860 sorghum 421 624 LYM455 sorghum|09v1|SB02G042460sorghum 422 625 LYM456 sorghum|09v1|SB03G000620 sorghum 423 626 LYM457sorghum|09v1|SB03G002840 sorghum 424 627 LYM458 sorghum|09v1|SB03G005490sorghum 425 628 LYM460 sorghum|09v1|SB03G010610 sorghum 426 775 LYM461sorghum|09v1|SB03G028800 sorghum 427 630 LYM463 sorghum|09v1|SB03G036240sorghum 428 776 LYM464 sorghum|09v1|SB03G037450 sorghum 429 632 LYM465sorghum|09v1|SB03G042320 sorghum 430 777 LYM466 sorghum|09v1|SB03G042690sorghum 431 778 LYM467 sorghum|09v1|SB03G044230 sorghum 432 635 LYM468sorghum|09v1|SB03G046070 sorghum 433 636 LYM471 sorghum|09v1|SB04G009670sorghum 434 779 LYM472 sorghum|09v1|SB04G017800 sorghum 435 780 LYM473sorghum|09v1|SB04G020170 sorghum 436 639 LYM474 sorghum|09v1|SB04G022570sorghum 437 640 LYM475 sorghum|09v1|SB04G023155 sorghum 438 781 LYM476sorghum|09v1|SB04G028950 sorghum 439 642 LYM477 sorghum|09v1|SB04G030560sorghum 440 643 LYM478 sorghum|09v1|SB05G000940 sorghum 441 644 LYM480sorghum|09v1|SB05G001550 sorghum 442 646 LYM481 sorghum|09v1|SB05G005450sorghum 443 782 LYM483 sorghum|09v1|SB05G018376 sorghum 444 783 LYM484sorghum|09v1|SB05G019020 sorghum 445 649 LYM485 sorghum|09v1|SB06G021970sorghum 446 650 LYM486 sorghum|09v1|SB06G024300 sorghum 447 651 LYM487sorghum|09v1|SB06G027830 sorghum 448 652 LYM488 sorghum|09v1|SB06G029440sorghum 449 784 LYM489 sorghum|09v1|SB06G030740 sorghum 450 654 LYM490sorghum|09v1|SB06G032170 sorghum 451 655 LYM491 sorghum|09v1|SB06G033090sorghum 452 656 LYM492 sorghum|09v1|SB07G001470 sorghum 453 657 LYM493sorghum|09v1|SB07G003070 sorghum 454 785 LYM494 sorghum|09v1|SB07G005420sorghum 455 659 LYM495 sorghum|09v1|SB07G027350 sorghum 456 660 LYM496sorghum|09v1|SB07G027880 sorghum 457 786 LYM497 sorghum|09v1|SB08G000390sorghum 458 662 LYM498 sorghum|09v1|SB08G000930 sorghum 459 663 LYM499sorghum|09v1|SB08G002960 sorghum 460 787 LYM500 sorghum|09v1|SB08G007640sorghum 461 788 LYM501 sorghum|09v1|SB08G009120 sorghum 462 789 LYM502sorghum|09v1|SB08G019150 sorghum 463 667 LYM503 sorghum|09v1|SB08G019960sorghum 464 668 LYM504 sorghum|09v1|SB08G022310 sorghum 465 669 LYM505sorghum|09v1|SB09G004700 sorghum 466 670 LYM506 sorghum|09v1|SB10G023650sorghum 467 671 LYM507 sorghum|09v1|SB10G023690 sorghum 468 672 LYM509sorghum|09v1|SB10G029550 sorghum 469 674 LYM510 wheat|gb164|CA745761wheat 470 790 LYM304_H3 brachypodium|09v1|DV468923 brachypodium 471 676LYM307_H7 sorghum|09v1|SB01G033760 sorghum 472 791 LYM326_H4brachypodium|09v1|GT790559 brachypodium 473 678 LYM368_H4sorghum|09v1|SB04G022750 sorghum 474 679 LYM397_H2sorghum|09v1|SB04G036540 sorghum 475 792 LYM311barley|gb157SOLEXA|AV909117 barley 476 — LYM325barley|gb157SOLEXA|BF623560 barley 477 — LYM420 sorghum|09v1|AW287430sorghum 478 — LYM422 sorghum|09v1|AW745990 sorghum 479 — LYM432sorghum|09v1|CF073969 sorghum 480 — Table 1: Provided are the identifiedgenes, their annotation, organism and polynucleotide and polypeptidesequence identifiers. “polyn.” = polynucleotide; “polyp.” = polypeptide.

Example 2 Identification of Homologous Sequences that Increase Yield,Fiber Yield, Fiber Quality, Growth Rate, Biomass, Oil Content, Vigor,Abst, and/or Nue of a Plant

The concepts of orthology and paralogy have recently been applied tofunctional characterizations and classifications on the scale ofwhole-genome comparisons. Orthologs and paralogs constitute two majortypes of homologs: The first evolved from a common ancestor byspecialization, and the latter are related by duplication events. It isassumed that paralogs arising from ancient duplication events are likelyto have diverged in function while true orthologs are more likely toretain identical function over evolutionary time.

To identify putative orthologs of the genes affecting plant yield, oilyield, oil content, seed yield, growth rate, vigor, biomass, abioticstress tolerance, and fertilizer use efficiency (FUE) genes and/ornitrogen use efficiency, all sequences were aligned using the BLAST(Basic Local Alignment Search Tool). Sequences sufficiently similar weretentatively grouped. These putative orthologs were further organizedunder a Phylogram—a branching diagram (tree) assumed to be arepresentation of the evolutionary relationships among the biologicaltaxa. Putative ortholog groups were analyzed as to their agreement withthe phylogram and in cases of disagreements these ortholog groups werebroken accordingly.

Expression data was analyzed and the EST libraries were classified usinga fixed vocabulary of custom terms such as developmental stages (e.g.,genes showing similar expression profile through development with upregulation at specific stage, such as at the seed filling stage) and/orplant organ (e.g., genes showing similar expression profile across theirorgans with up regulation at specific organs such as seed). Theannotations from all the ESTs clustered to a gene were analyzedstatistically by comparing their frequency in the cluster versus theirabundance in the database, allowing the construction of a numeric andgraphic expression profile of that gene, which is termed “digitalexpression”. The rationale of using these two complementary methods withmethods of phenotypic association studies of QTLs, SNPs and phenotypeexpression correlation is based on the assumption that true orthologsare likely to retain identical function over evolutionary time. Thesemethods provide different sets of indications on function similaritiesbetween two homologous genes, similarities in the sequencelevel-identical amino acids in the protein domains and similarity inexpression profiles.

The search and identification of homologous genes involves the screeningof sequence information available, for example, in public databases suchas the DNA Database of Japan (DDBJ), Genbank, and the European MolecularBiology Laboratory Nucleic Acid Sequence Database (EMBL) or versionsthereof or the MIPS database. A number of different search algorithmshave been developed, including but not limited to the suite of programsreferred to as BLAST programs. There are five implementations of BLAST,three designed for nucleotide sequence queries (BLASTN, BLASTX, andTBLASTX) and two designed for protein sequence queries (BLASTP andTBLASTN) (Coulson, Trends in Biotechnology: 76-80, 1994; Birren et al.,Genome Analysis, I: 543, 1997). Such methods involve alignment andcomparison of sequences. The BLAST algorithm calculates percent sequenceidentity and performs a statistical analysis of the similarity betweenthe two sequences. The software for performing BLAST analysis ispublicly available through the National Centre for BiotechnologyInformation. Other such software or algorithms are GAP, BESTFIT, FASTAand TFASTA. GAP uses the algorithm of Needleman and Wunsch (J. Mol.Biol. 48: 443-453, 1970) to find the alignment of two complete sequencesthat maximizes the number of matches and minimizes the number of gaps.

The homologous genes may belong to the same gene family. The analysis ofa gene family may be carried out using sequence similarity analysis. Toperform this analysis one may use standard programs for multiplealignments e.g. Clustal W. A neighbour-joining tree of the proteinshomologous to the genes in this invention may be used to provide anoverview of structural and ancestral relationships. Sequence identitymay be calculated using an alignment program as described above. It isexpected that other plants will carry a similar functional gene(ortholog) or a family of similar genes and those genes will provide thesame preferred phenotype as the genes presented here. Advantageously,these family members may be useful in the methods of the invention.Example of other plants are included here but not limited to, barley(Hordeum vulgare), Arabidopsis (Arabidopsis thaliana), maize (Zea mays),cotton (Gossypium), Oilseed rape (Brassica napus), Rice (Oryza sativa),Sugar cane (Saccharum officinarum), Sorghum (Sorghum bicolor), Soybean(Glycine max), Sunflower (Helianthus annuus), Tomato (Lycopersiconesculentum), Wheat (Triticum aestivum).

The above-mentioned analyses for sequence homology can be carried out ona full-length sequence, but may also be based on a comparison of certainregions such as conserved domains. The identification of such domains,would also be well within the realm of the person skilled in the art andwould involve, for example, a computer readable format of the nucleicacids of the present invention, the use of alignment software programsand the use of publicly available information on protein domains,conserved motifs and boxes. This information is available in the PRODOM(Hypertext Transfer Protocol://World Wide Web (dot) biochem (dot) ucl(dot) ac (dot) uk/bsm/dbbrowser/protocol/prodomqry (dot) html), PR(Hypertext Transfer Protocol://pir (dot) Georgetown (dot) edu/) or Pfam(Hypertext Transfer Protocol://World Wide Web (dot) sanger (dot) ac(dot) uk/Software/Pfam/) database. Sequence analysis programs designedfor motif searching may be used for identification of fragments, regionsand conserved domains as mentioned above. Preferred computer programsinclude, but are not limited to, MEME, SIGNALSCAN, and GENESCAN.

A person skilled in the art may use the homologous sequences providedherein to find similar sequences in other species and other organisms.Homologues of a protein encompass, peptides, oligopeptides,polypeptides, proteins and enzymes having amino acid substitutions,deletions and/or insertions relative to the unmodified protein inquestion and having similar biological and functional activity as theunmodified protein from which they are derived. To produce suchhomologues, amino acids of the protein may be replaced by other aminoacids having similar properties (conservative changes, such as similarhydrophobicity, hydrophilicity, antigenicity, propensity to form orbreak a-helical structures or 3-sheet structures). Conservativesubstitution tables are well known in the art (see for example Creighton(1984) Proteins. W.H. Freeman and Company). Homologues of a nucleic acidencompass nucleic acids having nucleotide substitutions, deletionsand/or insertions relative to the unmodified nucleic acid in questionand having similar biological and functional activity as the unmodifiednucleic acid from which they are derived.

Table 2, hereinbelow, lists a summary of orthologous and homologoussequences of the polynucleotide sequences (SEQ ID NOs:1-288 and 289-480)and polypeptide sequences (SEQ ID NOs:481-727 and 728-792) presented inTable 1 above and in Table 32 below, which were identified from thedatabases using the NCBI BLAST software (e.g., using the Blastp andtBlastn algorithms) and needle (EMBOSS package) as being at least 80%homologous to the selected polynucleotides and polypeptides, and whichare expected to increase plant yield, seed yield, oil yield, oilcontent, growth rate, fiber yield, fiber quality, biomass, vigor, ABSTand/or NUE of a plant.

TABLE 2 Homologues of the identified genes/polypeptides for increasingyield, fiber yield, fiber quality, growth rate, vigor, biomass, growthrate, abiotic stress tolerance, nitrogen use efficiency, water useefficiency and fertilizer use efficiency of a plant Nucl. Polyp. Hom. to% SEQ ID Hom. to SEQ ID SEQ ID global NO: Gene Name cluster name NO: NO:iden. Algor. 793 LYM298 arabidopsis_lyrata|09v1|JGIAL008806_P1 2947 48394.4 globlastp 794 LYM299 arabidopsis_lyrata|09v1|JGIAL028327_P1 2948484 95 globlastp 795 LYM300 wheat|gb164|BM135033 2949 485 82.1 globlastp796 LYM300 wheat|10v2|BE430200_P1 2949 485 82.1 globlastp 797 LYM300wheat|gb164|BE430200 2950 485 81.91 glotblastn 798 LYM300wheat|gb164|BE606947 2951 485 80 globlastp 799 LYM302wheat|10v2|BE415864_P1 2952 487 91.2 globlastp 800 LYM302wheat|gb164|BE415864 2953 487 90.4 globlastp 801 LYM302oat|10v2|GO592242_P1 2954 487 82.6 globlastp 802 LYM304wheat|10v2|BE604238_T1 2955 489 97.7 glotblastn 802 LYM304_H3wheat|10v2|BE604238_P1 2955 676 90.9 globlastp 803 LYM304wheat|gb164|BE604238 2956 489 97.13 glotblastn 803 LYM304_H3wheat|gb164|BE604238 2956 676 87.3 globlastp 804 LYM304wheat|10v2|AL825256_T1 2957 489 95.98 glotblastn 805 LYM304wheat|gb164|CA607048 2958 489 95.98 glotblastn 806 LYM304oat|10v2|CN816603_T1 2959 489 91.95 glotblastn 806 LYM304_H3oat|10v2|CN816603_P1 2959 676 91.5 globlastp 807 LYM304wheat|gb164|AL822468 2960 489 90.8 globlastp 808 LYM304fescue|gb161|DT681301_T1 2961 489 88.51 glotblastn 809 LYM304rice|gb170|OS06G10770 2962 489 85.63 glotblastn 809 LYM304_H3rice|gb170|OS06G10770 2962 676 81.2 globlastp 810 LYM304sorghum|09v1|SB10G006970 2963 489 84.48 glotblastn 810 LYM304_H3sorghum|09v1|SB10G006970 2963 676 80.7 globlastp 811 LYM304foxtail_millet|10v2|FXTRMSLX00901077D1_T1 2964 489 83.33 glotblastn 812LYM304 millet|10v1|EVO454PM128453_T1 2965 489 83.33 glotblastn 812LYM304_H3 millet|10v1|EVO454PM128453_P1 2965 676 80 globlastp 813 LYM304sugarcane|10v1|BQ529603_T1 2966 489 82.76 glotblastn 814 LYM304sugarcane|gb157.3|BQ529603 2967 489 82.76 glotblastn 815 LYM304millet|09v1|EVO454PM128453 2968 489 82.2 globlastp 816 LYM304wheat|10v2|CA485187_P1 2969 489 82.2 globlastp 817 LYM304switchgrass|gb167|FE628239 2970 489 82.18 glotblastn 818 LYM304wheat|gb164|CA485187 2971 489 81.6 globlastp 819 LYM305barley|10v2|BE413415_P1 2972 490 94.9 globlastp 820 LYM306wheat|10v2|BF482499_P1 2973 491 95.6 globlastp 821 LYM306wheat|gb164|BF482499 2974 491 91.6 globlastp 822 LYM306brachypodium|09v1|DV471800_P1 2975 491 85.5 globlastp 823 LYM306oat|10v2|GR314164_P1 2976 491 85.2 globlastp 824 LYM307wheat|10v2|CA497658_T1 2977 492 98.65 glotblastn 825 LYM307wheat|gb164|CA497658 2978 492 98.65 glotblastn 826 LYM307brachypodium|09v1|GT768682_T1 2979 492 97.3 glotblastn 826 LYM307_H7brachypodium|09v1|GT768682_P1 2979 791 84.7 globlastp 827 LYM307rice|gb170|OS03G26960 2980 492 94.59 glotblastn 827 LYM307_H7rice|gb170|OS03G26960 2980 791 86.3 globlastp 828 LYM307millet|09v1|CD724364 2981 492 93.69 glotblastn 829 LYM307millet|10v1|CD724364_T1 2982 492 93.69 glotblastn 830 LYM307switchgrass|gb167|FE618254 2983 492 93.24 glotblastn 831 LYM307maize|10v1|AI941642_T1 2984 492 92.34 glotblastn 831 LYM307_H7maize|10v1|AI941642_T1 2984 677 93.58 glotblastn 832 LYM307maize|gb170|AI941642 2985 492 92.34 glotblastn 832 LYM307_H7maize|gb170|AI941642 2985 791 93.4 globlastp 833 LYM307prunus|10v1|BU046517_T1 2986 492 90.54 glotblastn 834 LYM307strawberry|11v1|CO818023_T1 2987 492 89.19 glotblastn 835 LYM307cassava|09v1|DB921661_T1 2988 492 88.74 glotblastn 836 LYM307cacao|10v1|CU590610_T1 2989 492 88.29 glotblastn 837 LYM307oak|10v1|FP035476_T1 2990 492 88.29 glotblastn 838 LYM307pigeonpea|10v1|SRR054580S0008307_T1 2991 492 87.84 glotblastn 839 LYM307sequoia|10v1|SRR065044S0120822_T1 2992 492 87.84 glotblastn 840 LYM307medicago|09v1|BF631940_T1 2993 492 87.84 glotblastn 841 LYM307cotton|10v2|AI728035_T1 2994 492 87.39 glotblastn 842 LYM307castorbean|09v1|GE633986_T1 2995 492 87.39 glotblastn 843 LYM307podocarpus|10v1|SRR065014S0002749_T1 2996 492 86.94 glotblastn 844LYM307 cowpea|gb166|FG829824_T1 2997 492 86.94 glotblastn 845 LYM307poplar|10v1|BU893016_T1 2998 492 86.94 glotblastn 846 LYM307poplar|gb170|BU893016 2999 492 86.94 glotblastn 847 LYM307arabidopsis|10v1|AT2G38770_T1 3000 492 86.94 glotblastn 848 LYM307soybean|11v1|GLYMA20G00260_T1 3001 492 86.94 glotblastn 849 LYM307soybean|gb168|AW587177 3001 492 86.94 glotblastn 850 LYM307lotus|09v1|BP085687_T1 3002 492 86.49 glotblastn 851 LYM307soybean|11v1|GLYMA0048S00340_T1 3003 492 86.49 glotblastn 852 LYM307soybean|gb168|BE822147 3004 492 86.49 glotblastn 853 LYM307arabidopsis_lyrata|09v1|JGIAL015120_T1 3005 492 86.49 glotblastn 854LYM307 aquilegia|10v2|DT738373_T1 3006 492 86.49 glotblastn 855 LYM307canola|10v1|BG732277_T1 3007 492 86.04 glotblastn 856 LYM307pine|10v2|BE662622_T1 3008 492 85.59 glotblastn 857 LYM307canola|gb161|BG732277 3009 492 85.59 glotblastn 858 LYM307solanum_phureja|09v1|SPHAW398539 3010 492 85.59 glotblastn 859 LYM307tomato|09v1|AW398539 3011 492 85.59 glotblastn 860 LYM307cucumber|09v1|BGI454G0037397_T1 3012 492 85.59 glotblastn 861 LYM307citrus|gb166|CB291414_T1 3013 492 85.14 glotblastn 862 LYM307foxtail_millet|10v2|FXTRMSLX04048331D1_T1 3014 492 83.33 glotblastn 863LYM307 monkeyflower|10v1|GO948235_T1 3015 492 81.98 glotblastn 864LYM307 monkeyflower|10v1|GO948236_T1 3016 492 81.98 glotblastn 865LYM307 aristolochia|10v1|SRR039082S0072841_T1 3017 492 80.63 glotblastn866 LYM307 aquilegia|gb157.3|DT738373 3018 492 80.63 glotblastn 867LYM309 wheat|10v2|BE500784_P1 3019 494 96.4 globlastp 867 LYM495wheat|10v2|BE500784_P1 3019 660 82.3 globlastp 868 LYM309wheat|gb164|BF200740 3020 494 87.9 globlastp 868 LYM495wheat|gb164|BF200740 3020 660 82.63 glotblastn 869 LYM309brachypodium|09v1|GT789184_P1 3021 494 87.2 globlastp 869 LYM495brachypodium|09v1|GT789184_P1 3021 660 83.6 globlastp 870 LYM309pseudoroegneria|gb167|FF342430_P1 3022 494 86.5 globlastp 871 LYM309rice|gb170|OS08G40430 3023 494 84.7 globlastp 871 LYM495rice|gb170|OS08G40430 3023 660 88.9 globlastp 872 LYM309rice|gb170|OS08G40420 3024 494 84.68 glotblastn 872 LYM495rice|gb170|OS08G40420 3024 660 88.92 glotblastn 873 LYM309wheat|gb164|AL817405 3025 494 84.5 globlastp 873 LYM495wheat|gb164|AL817405 3025 660 82.34 glotblastn 874 LYM309switchgrass|gb167|FE651785 3026 494 80.18 glotblastn 874 LYM495switchgrass|gb167|FE651785 3026 660 91.9 globlastp 875 LYM313rye|gb164|BE493923 3027 497 89.6 globlastp 876 LYM313wheat|10v2|CA498090_P1 3028 497 88.1 globlastp 877 LYM313wheat|gb164|CA498090 3028 497 88.1 globlastp 878 LYM313wheat|10v2|BE637619_P1 3029 497 87.7 globlastp 879 LYM313wheat|gb164|BE637619 3030 497 86.2 globlastp 880 LYM314leymus|gb166|EG376544_P1 3031 498 93.7 globlastp 881 LYM314wheat|10v2|BE403164_P1 3032 498 89.6 globlastp 882 LYM314wheat|gb164|BE403164 3032 498 89.6 globlastp 883 LYM314wheat|10v2|BE404241_P1 3033 498 89.2 globlastp 884 LYM314wheat|gb164|BE404241 3033 498 89.2 globlastp 885 LYM314wheat|10v2|BE405115_P1 3034 498 89 globlastp 886 LYM314wheat|gb164|BE405115 3034 498 89 globlastp 887 LYM315oat|10v2|BE439287_P1 3035 499 86.8 globlastp 888 LYM315brachypodium|09v1|DV475843_P1 3036 499 86.5 globlastp 889 LYM315rice|gb170|OS01G01790 3037 499 82.3 globlastp 890 LYM316wheat|10v2|BI750788_P1 3038 500 97.7 globlastp 891 LYM316wheat|gb164|BI750788 3039 500 97.7 globlastp 892 LYM316brachypodium|09v1|GT775994_P1 3040 500 96.2 globlastp 893 LYM316sorghum|09v1|SB08G023070 3041 500 94.9 globlastp 894 LYM316rice|gb170|OS12G44150 3042 500 94.7 globlastp 895 LYM316maize|10v1|AI947455_P1 3043 500 94.7 globlastp 896 LYM316brachypodium|09v1|DV485303_P1 3044 500 92.7 globlastp 897 LYM316rice|gb170|OS03G48310 3045 500 92.3 globlastp 898 LYM316maize|gb170|AI444726 3046 500 92 globlastp 899 LYM316maize|10v1|AI444726_P1 3047 500 91.3 globlastp 900 LYM316solanum_phureja|09v1|SPHTOMLHA1 3048 500 89.8 globlastp 901 LYM316tomato|09v1|TOMLHA1 3049 500 89.68 glotblastn 902 LYM316maize|10v1|ZMU08984_P1 3050 500 89.5 globlastp 903 LYM316maize|gb170|ZMU08984 3050 500 89.5 globlastp 904 LYM316soybean|11v1|GLYMA05G01460_P1 3051 500 89.2 globlastp 905 LYM316soybean|gb168|BQ137671 3051 500 89.2 globlastp 906 LYM316cacao|10v1|CU477584_T1 3052 500 89.05 glotblastn 907 LYM316oak|10v1|CU640330_P1 3053 500 89 globlastp 908 LYM316strawberry|11v1|CO379666_P1 3054 500 89 globlastp 909 LYM316cucumber|09v1|DV634280_P1 3055 500 89 globlastp 910 LYM316cucumber|09v1|BGI454G0068170_P1 3056 500 89 globlastp 911 LYM316soybean|11v1|GLYMA17G10420_P1 3057 500 88.9 globlastp 912 LYM316soybean|11v1|GLYMA06G20200_P1 3058 500 88.9 globlastp 913 LYM316soybean|gb168|BE823826 3058 500 88.9 globlastp 914 LYM316prunus|gb167|CB818450 3059 500 88.9 globlastp 915 LYM316walnuts|gb166|AY347715 3060 500 88.8 globlastp 916 LYM316soybean|11v1|GLYMA04G34370_P1 3061 500 88.8 globlastp 917 LYM316soybean|gb168|BQ453861 3061 500 88.8 globlastp 918 LYM316tomato|09v1|AF275745 3062 500 88.74 glotblastn 919 LYM316cucumber|09v1|BGI454G0029194_P1 3063 500 88.7 globlastp 920 LYM316rice|gb170|OS07G09340 3064 500 88.7 globlastp 921 LYM316sorghum|09v1|SB02G005440 3065 500 88.7 globlastp 922 LYM316solanum_phureja|09v1|SPHAF275745 3066 500 88.7 globlastp 923 LYM316potato|10v1|BF459938_P1 3067 500 88.6 globlastp 924 LYM316potato|gb157.2|BF459938 3067 500 88.6 globlastp 925 LYM316aquilegia|10v2|DR912485_P1 3068 500 88.5 globlastp 926 LYM316sunflower|10v1|CD855840_P1 3069 500 88.5 globlastp 927 LYM316millet|10v1|EVO454PM000746_P1 3070 500 88.5 globlastp 928 LYM316pine|10v2|AW226212_P1 3071 500 88.4 globlastp 929 LYM316taxus|10v1|SRR032523S0008818_P1 3072 500 88.4 globlastp 930 LYM316arabidopsis|10v1|AT5G62670_P1 3073 500 88.4 globlastp 931 LYM316arabidopsis|gb165|AT5G62670 3073 500 88.4 globlastp 932 LYM316orobanche|10v1|SRR023189S0000310_P1 3074 500 88.3 globlastp 933 LYM316sciadopitys|10v1|SRR065035S0004583_P1 3075 500 88.3 globlastp 934 LYM316poplar|10v1|AI166273_P1 3076 500 88.3 globlastp 935 LYM316poplar|gb170|AI166273 3076 500 88.3 globlastp 936 LYM316soybean|gb168|CD410987 3077 500 88.3 globlastp 937 LYM316pseudotsuga|10v1|SRR065119S0000105_P1 3078 500 88.2 globlastp 938 LYM316brachypodium|09v1|GT769251_P1 3079 500 88.2 globlastp 939 LYM316cotton|10v2|SRR032367S0004261_P1 3080 500 88.1 globlastp 940 LYM316triphysaria|gb164|BE574923 3081 500 88 globlastp 941 LYM316cassava|09v1|JGICASSAVA3457M1_P1 3082 500 88 globlastp 942 LYM316poplar|10v1|BU821931_P1 3083 500 87.9 globlastp 943 LYM316cotton|10v2|CO084073_P1 3084 500 87.8 globlastp 944 LYM316poplar|gb170|BU821931 3085 500 87.8 globlastp 945 LYM316monkeyflower|10v1|SRR037227S0002431_P1 3086 500 87.7 globlastp 946LYM316 chestnut|gb170|SRR006295S0001140_P1 3087 500 87.7 globlastp 947LYM316 prunus|10v1|CB821619_P1 3088 500 87.5 globlastp 948 LYM316monkeyflower|10v1|DV206482_P1 3089 500 87.3 globlastp 949 LYM316castorbean|09v1|XM002517411_P1 3090 500 87.2 globlastp 950 LYM316sunflower|10v1|DY937446_P1 3091 500 87.2 globlastp 951 LYM316arabidopsis|10v1|AT3G47950_P1 3092 500 87.1 globlastp 952 LYM316foxtail_millet|10v2|OXEC612066T1_P1 3093 500 86.8 globlastp 953 LYM316soybean|11v1|GLYMA19G02270_P1 3094 500 86.5 globlastp 954 LYM316cacao|10v1|CF972872_P1 3095 500 86.4 globlastp 955 LYM316switchgrass|gb167|FL749584 3096 500 86.2 globlastp 956 LYM316strawberry|11v1|SRR034859S0009654_P1 3097 500 85.2 globlastp 957 LYM316aristolochia|10v1|SRR039082S0479301_P1 3098 500 84.3 globlastp 958LYM316 pine|10v2|CX649213_T1 3099 500 84.13 glotblastn 959 LYM316cassava|09v1|DV444631_T1 3100 500 84.04 glotblastn 960 LYM316foxtail_millet|10v2|OXEC613731T1_P1 3101 500 83.1 globlastp 961 LYM316maize|10v1|GFXZMU09989X1_P1 3102 500 82.9 globlastp 962 LYM316soybean|11v1|BM567790_P1 3103 500 82.4 globlastp 963 LYM316soybean|gb168|BF634181 3104 500 82.4 globlastp 964 LYM316podocarpus|10v1|SRR065014S0001544_P1 3105 500 82.3 globlastp 965 LYM316cleome_gynandra|10v1|SRR015532S0001594_P1 3106 500 81.4 globlastp 966LYM316 cassava|09v1|DV445742_P1 3107 500 81.3 globlastp 967 LYM316poplar|10v1|AY165042_P1 3108 500 80.9 globlastp 968 LYM316soybean|11v1|GLYMA14G17360_P1 3109 500 80.9 globlastp 969 LYM316banana|10v1|GFXFN396603X3_P1 3110 500 80.7 globlastp 970 LYM316castorbean|09v1|EG674264_P1 3111 500 80.7 globlastp 971 LYM316monkeyflower|10v1|GR053706_P1 3112 500 80.7 globlastp 972 LYM316sorghum|09v1|SB06G031240_P1 3113 500 80.7 globlastp 973 LYM316soybean|11v1|GLYMA17G29370_T1 3114 500 80.6 glotblastn 974 LYM316sugarcane|10v1|BQ532941_T1 3115 500 80.54 glotblastn 975 LYM316maize|10v1|AI001235_P1 3116 500 80.5 globlastp 976 LYM316oak|10v1|DN950122_P1 3117 500 80.5 globlastp 977 LYM316chestnut|gb170|SRR006295S0006926_P1 3118 500 80.4 globlastp 978 LYM316foxtail_millet|10v2|SICRP039993_P1 3119 500 80.4 globlastp 979 LYM316maize|10v1|AI615212_P1 3120 500 80.4 globlastp 980 LYM316poplar|10v1|BU884336_P1 3121 500 80.4 globlastp 981 LYM316rice|gb170|OS04G56160_P1 3122 500 80.4 globlastp 982 LYM316soybean|11v1|GLYMA09G06250_P1 3123 500 80.4 globlastp 983 LYM316soybean|11v1|GLYMA15G17530_P1 3124 500 80.4 globlastp 984 LYM316potato|10v1|BF459991_P1 3125 500 80.3 globlastp 985 LYM316pseudotsuga|10v1|SRR065119S0010587_P1 3126 500 80.2 globlastp 986 LYM316solanum_phureja|09v1|SPHTOMTRALTBL_P1 3127 500 80.2 globlastp 987 LYM316soybean|11v1|GLYMA13G00840_P1 3128 500 80.2 globlastp 988 LYM316cacao|10v1|CA796153_P1 3129 500 80.1 globlastp 989 LYM316cacao|10v1|CU477696_P1 3130 500 80.1 globlastp 990 LYM316cotton|10v2|CO113314_P1 3131 500 80.1 globlastp 991 LYM316cucumber|09v1|AJ703811_P1 3132 500 80.1 globlastp 992 LYM316monkeyflower|10v1|DV206165_P1 3133 500 80.1 globlastp 993 LYM316orobanche|10v1|SRR023189S0000837_P1 3134 500 80.1 globlastp 994 LYM316physcomitrella|10v1|AW700088_P1 3135 500 80.1 globlastp 995 LYM316poplar|10v1|BI071253_P1 3136 500 80.1 globlastp 996 LYM316prunus|10v1|CN491211_P1 3137 500 80.1 globlastp 997 LYM316arabidopsis_lyrata|09v1|CRPALE021507_P1 3138 500 80 globlastp 998 LYM316arabidopsis|10v1|AT4G30190_P1 3139 500 80 globlastp 999 LYM316cotton|10v2|CO113293_P1 3140 500 80 globlastp 1000 LYM316millet|10v1|DQ875455_P1 3141 500 80 globlastp 1001 LYM316tomato|09v1|TOMTRALTBL_P1 3142 500 80 globlastp 1002 LYM317wheat|10v2|BE428966_P1 3143 501 97.7 globlastp 1003 LYM317pseudoroegneria|gb167|FF343824 3144 501 97.4 globlastp 1004 LYM317leymus|gb166|EG376251_P1 3145 501 97.4 globlastp 1005 LYM317leymus|gb166|EG375010_P1 3146 501 97.1 globlastp 1006 LYM317brachypodium|09v1|DV469643_P1 3147 501 94.2 globlastp 1007 LYM317sugarcane|gb157.3|CA085456 3148 501 88.6 globlastp 1008 LYM317sugarcane|gb157.3|BQ536025 3148 501 88.6 globlastp 1009 LYM317sugarcane|gb157.3|CA071453 3149 501 88.4 globlastp 1010 LYM317sorghum|09v1|SB01G036580 3150 501 88.1 globlastp 1011 LYM317cenchrus|gb166|BM084530_P1 3151 501 86.9 globlastp 1012 LYM317maize|10v1|AI861382_P1 3152 501 86.6 globlastp 1013 LYM317maize|gb170|AI861382 3152 501 86.6 globlastp 1014 LYM317switchgrass|gb167|DN142628 3153 501 86.3 globlastp 1015 LYM317switchgrass|gb167|DN140729 3154 501 86 globlastp 1016 LYM317rice|gb170|OS03G21040T2 3155 501 85.4 globlastp 1017 LYM317foxtail_millet|10v2|SICRP025043_T1 3156 501 84.86 glotblastn 1018 LYM317sugarcane|gb157.3|CA112033 3157 501 82.18 glotblastn 1019 LYM318wheat|10v2|BE352604_P1 3158 502 97.1 globlastp 1020 LYM318wheat|gb164|BE352604 3159 502 94.6 globlastp 1021 LYM318switchgrass|gb167|DN145977 3160 502 93.5 globlastp 1022 LYM318foxtail_millet|10v2|SICRP022490_P1 3161 502 93.3 globlastp 1023 LYM318millet|10v1|EVO454PM001279_P1 3162 502 92.9 globlastp 1024 LYM318switchgrass|gb167|DN143219 3163 502 92.7 globlastp 1025 LYM318brachypodium|09v1|DV476722_P1 3164 502 92.3 globlastp 1026 LYM318maize|10v1|AI737467_P1 3165 502 92.3 globlastp 1027 LYM318rice|gb170|OS08G43640 3166 502 91.9 globlastp 1028 LYM318sorghum|09v1|SB07G024800 3167 502 91.7 globlastp 1029 LYM318sugarcane|gb157.3|BU103272 3168 502 91.6 globlastp 1030 LYM318maize|10v1|AI947388_P1 3169 502 91.4 globlastp 1031 LYM318maize|gb170|AI947388 3169 502 91.4 globlastp 1032 LYM318rice|gb170|OS09G37000 3170 502 89.2 globlastp 1033 LYM318switchgrass|gb167|FE599643 3171 502 88.5 globlastp 1034 LYM318brachypodium|09v1|DV471273_P1 3172 502 88.5 globlastp 1035 LYM318wheat|gb164|BE399426 3173 502 84.48 glotblastn 1036 LYM318oat|10v2|GO589547_P1 3174 502 83.6 globlastp 1037 LYM318millet|09v1|EVO454PM001279 3175 502 81.6 glotblastn 1038 LYM318oak|10v1|FP029519_P1 3176 502 81.4 globlastp 1039 LYM318cacao|10v1|CF974024_P1 3177 502 80.8 globlastp 1040 LYM318chestnut|gb170|SRR006295S0011484_P1 3178 502 80.8 globlastp 1041 LYM318soybean|11v1|GLYMA04G02370_P1 3179 502 80.8 globlastp 1042 LYM318soybean|gb168|BE660782 3179 502 80.8 globlastp 1043 LYM318momordica|10v1|SRR071315S0003995_P1 3180 502 80.6 globlastp 1044 LYM318nasturtium|10v1|GH171179_P1 3181 502 80.4 globlastp 1045 LYM318poplar|gb170|BI129079 3182 502 80.4 globlastp 1046 LYM318medicago|09v1|AW695167_P1 3183 502 80.3 globlastp 1047 LYM318melon|10v1|DV632592_P1 3184 502 80.2 globlastp 1048 LYM318peanut|10v1|EE126134_P1 3185 502 80.2 globlastp 1049 LYM318tobacco|gb162|AB001422 3186 502 80.2 globlastp 1050 LYM318poplar|10v1|BU811347_P1 3187 502 80.2 globlastp 1051 LYM318poplar|gb170|BU811347 3187 502 80.2 globlastp 1052 LYM318sunflower|10v1|CD850830_P1 3188 502 80.2 globlastp 1053 LYM318sunflower|gb162|CD850830 3188 502 80.2 globlastp 1054 LYM318cassava|09v1|DV441286_P1 3189 502 80.2 globlastp 1055 LYM318artemisia|gb164|EY080009 3190 502 80.2 globlastp 1056 LYM318cowpea|gb166|FF388382_P1 3191 502 80.2 globlastp 1057 LYM318soybean|11v1|GLYMA06G02410_P1 3192 502 80.2 globlastp 1058 LYM318soybean|gb168|AW695167 3192 502 80.2 globlastp 1059 LYM318castorbean|09v1|EG677995_T1 3193 502 80 glotblastn 1060 LYM319wheat|gb164|BE419309 3194 503 98.2 globlastp 1061 LYM319wheat|gb164|BE443380 3195 503 98.2 globlastp 1062 LYM319wheat|10v2|BE419309_P1 3195 503 98.2 globlastp 1063 LYM319wheat|10v2|BE428077_P1 3196 503 96.8 globlastp 1064 LYM319oat|10v2|GR322926_P1 3197 503 94.7 globlastp 1065 LYM319brachypodium|09v1|GT759567_P1 3198 503 93.7 globlastp 1066 LYM319rice|gb170|OS09G20350 3199 503 83.2 globlastp 1067 LYM320brachypodium|09v1|DV481104_P1 3200 504 84.1 globlastp 1068 LYM322sorghum|09v1|SB04G027090 3201 506 87.2 globlastp 1069 LYM322foxtail_millet|10v2|SICRP011275_T1 3202 506 86.2 glotblastn 1070 LYM322switchgrass|gb167|FE630202 3203 506 85.42 glotblastn 1071 LYM322maize|10v1|AI782996_P1 3204 506 84.6 globlastp 1072 LYM322maize|gb170|AI782996 3204 506 84.6 globlastp 1073 LYM322sugarcane|10v1|CA088583_T1 3205 506 83.85 glotblastn 1074 LYM322millet|10v1|EVO454PM036524_P1 3206 506 83.3 globlastp 1075 LYM322foxtail_millet|10v2|FXTRMSLX01164775D1_T1 3207 506 82.03 glotblastn 1076LYM323 wheat|gb164|AL827748 3208 507 95.4 globlastp 1077 LYM323wheat|gb164|DR737479 3209 507 84 glotblastn 1078 LYM323wheat|10v2|CJ616758_P1 3210 507 83.3 globlastp 1079 LYM323barley|10v2|BF622357_P1 3211 507 81.1 globlastp 1080 LYM323wheat|10v2|CA722056_P1 3212 507 81.1 globlastp 1081 LYM323wheat|10v2|BQ901526_P1 3213 507 80.1 globlastp 1082 LYM324wheat|gb164|BE404741 3214 508 91.6 globlastp 1083 LYM324wheat|gb164|BE606638 3215 508 91.6 globlastp 1084 LYM324wheat|10v2|BE404741_P1 3216 508 91 globlastp 1085 LYM324wheat|10v2|BE606638_P1 3217 508 90.4 globlastp 1086 LYM324brachypodium|09v1|GT806106_P1 3218 508 87.1 globlastp 1087 LYM324rice|gb170|OS01G07810 3219 508 83.2 globlastp 1088 LYM326leymus|gb166|EG400080_T1 3220 509 95.88 glotblastn 1089 LYM326wheat|10v2|BE445234_T1 3221 509 95.06 glotblastn 1089 LYM326_H4wheat|10v2|BE445234_P1 3221 678 80.3 globlastp 1090 LYM326wheat|gb164|BE445234 3222 509 95.06 glotblastn 1090 LYM326_H4wheat|gb164|BE445234 3222 678 80.26 glotblastn 1091 LYM326oat|10v2|GR328664_T1 3223 509 87.24 glotblastn 1092 LYM326maize|10v1|BG410328_T1 3224 509 86.01 glotblastn 1093 LYM326maize|gb170|BG410328 3224 509 86.01 glotblastn 1094 LYM326sorghum|09v1|SB04G001270 3225 509 85.19 glotblastn 1094 LYM326_H4sorghum|09v1|SB04G001270 3225 678 80.16 glotblastn 1095 LYM326rice|gb170|OS02G02520 3226 509 83.13 glotblastn 1096 LYM327wheat|10v2|BE425355_P1 3227 510 88.2 globlastp 1097 LYM327wheat|gb164|BE425355 3227 510 88.2 globlastp 1098 LYM327rye|gb164|BE586531 3228 510 87.1 globlastp 1099 LYM327wheat|10v2|BG608337_P1 3229 510 83.7 globlastp 1100 LYM327wheat|gb164|BG608337 3229 510 83.7 globlastp 1101 LYM327wheat|10v2|BE497048_P1 3230 510 81.6 globlastp 1102 LYM327wheat|gb164|BE497048 3230 510 81.6 globlastp 1103 LYM327wheat|gb164|DR739426 3231 510 80.61 glotblastn 1104 LYM327barley|10v2|BI949893_P1 3232 510 80.2 globlastp 1105 LYM327pseudoroegneria|gb167|FF365337 3233 510 80.2 globlastp 1106 LYM327wheat|10v2|BE402321_P1 3234 510 80 globlastp 1107 LYM330wheat|10v2|BE498332_P1 3235 513 88.9 globlastp 1108 LYM330wheat|gb164|BE498332 3235 513 88.9 globlastp 1109 LYM330pseudoroegneria|gb167|FF348503 3236 513 85.9 globlastp 1110 LYM331barley|gb157SOLEXA|AV910488 3237 514 83.7 globlastp 1111 LYM331rice|gb170|OS02G03720_P1 3238 514 81.2 globlastp 1112 LYM331brachypodium|09v1|GT789518_P1 3239 514 80.5 globlastp 1113 LYM332wheat|10v2|BE490464_P1 3240 515 95.2 globlastp 1114 LYM332wheat|10v2|CA597955_P1 3241 515 95.2 globlastp 1115 LYM332wheat|gb164|BQ743265 3242 515 95.2 globlastp 1116 LYM332brachypodium|09v1|DV484469_P1 3243 515 87.7 globlastp 1117 LYM332rice|gb170|OS04G44530 3244 515 87 globlastp 1118 LYM332foxtail_millet|10v2|FXTRMSLX00224883D1_P1 3245 515 85 globlastp 1119LYM332 millet|10v1|EVO454PM009535_P1 3246 515 84.8 globlastp 1120 LYM332maize|10v1|AW055525_P1 3247 515 83.4 globlastp 1121 LYM332maize|gb170|AW055525 3247 515 83.4 globlastp 1122 LYM332maize|10v1|AW018233_T1 3248 515 83.33 glotblastn 1123 LYM332maize|gb170|AW018233 3249 515 83.3 globlastp 1124 LYM332sorghum|09v1|SB06G023190 3250 515 83.1 globlastp 1125 LYM333wheat|10v2|AL827009_P1 3251 516 86.8 globlastp 1126 LYM333wheat|gb164|AL827009 3252 516 86.8 globlastp 1127 LYM334wheat|10v2|BG606663_P1 3253 517 89.5 globlastp 1128 LYM334wheat|gb164|BG606663 3254 517 88.8 globlastp 1129 LYM334oat|10v2|GR340052_P1 3255 517 80.7 globlastp 1130 LYM335wheat|10v2|BG608153_P1 3256 518 81 globlastp 1131 LYM341barley|10v2|BF255151_P1 3257 523 86.2 globlastp 1132 LYM341barley|gb157SOLEXA|BF255151 3258 523 85.9 globlastp 1133 LYM341wheat|10v2|BE489094_P1 3259 523 84.6 globlastp 1134 LYM341wheat|gb164|BE499583 3260 523 84.4 globlastp 1135 LYM342brachypodium|09v1|DV471725_P1 3261 524 81.9 globlastp 1136 LYM343oak|10v1|FP039541_P1 3262 525 80 globlastp 1137 LYM345cotton|10v2|DT544816_P1 3263 527 94.2 globlastp 1138 LYM345cacao|10v1|CU505040_P1 3264 527 85.8 globlastp 1139 LYM346maize|10v1|BE224797_P1 3265 528 97.3 globlastp 1140 LYM346maize|gb170|BE224797 3265 528 97.3 globlastp 1141 LYM346sugarcane|10v1|CA141777_P1 3266 528 96.2 globlastp 1142 LYM346sorghum|09v1|SB03G010800 3267 528 96.2 globlastp 1143 LYM346switchgrass|gb167|FE621427 3268 528 93.4 globlastp 1144 LYM346sugarcane|10v1|CA084777_P1 3269 528 86.6 globlastp 1145 LYM346sugarcane|gb157.3|CA084777 3269 528 86.6 globlastp 1146 LYM346oat|10v2|GO588228_P1 3270 528 84.8 globlastp 1147 LYM346brachypodium|09v1|DV476378_P1 3271 528 83.8 globlastp 1148 LYM348sorghum|09v1|SB06G030390 3272 529 95.8 globlastp 1149 LYM348foxtail_millet|10v2|FXTRMSLX00976092D1_P1 3273 529 93.4 globlastp 1150LYM348 barley|10v2|BF631070_P1 3274 529 89.3 globlastp 1151 LYM348wheat|gb164|BE425951 3275 529 88.77 glotblastn 1152 LYM348switchgrass|gb167|FL737932 3276 529 86 globlastp 1153 LYM348rice|gb170|OS04G55050 3277 529 85.7 globlastp 1154 LYM348brachypodium|09v1|DV470592_P1 3278 529 85.5 globlastp 1155 LYM349sorghum|09v1|SB03G044720 3279 530 95.7 globlastp 1156 LYM349rice|gb170|OS01G70390 3280 530 90.6 globlastp 1157 LYM349brachypodium|09v1|SRR031795S0016465_P1 3281 530 87.3 globlastp 1158LYM350 sorghum|09v1|SB10G031240 3282 531 95.8 globlastp 1159 LYM350sugarcane|10v1|CA092260_P1 3283 531 94.4 globlastp 1160 LYM350sugarcane|gb157.3|CA092260 3283 531 94.4 globlastp 1161 LYM350switchgrass|gb167|DN140794 3284 531 89.2 globlastp 1162 LYM350switchgrass|gb167|DN152334 3285 531 84.7 globlastp 1163 LYM350foxtail_millet|10v2|SICRP011626_P1 3286 531 83.4 globlastp 1164 LYM350millet|10v1|EVO454PM003260_T1 3287 531 82.89 glotblastn 1165 LYM351sorghum|09v1|SB08G020890 3288 532 91.5 globlastp 1166 LYM351switchgrass|gb167|FL770825 3289 532 89.38 glotblastn 1167 LYM351millet|10v1|EVO454PM052672_P1 3290 532 87.9 globlastp 1168 LYM351rice|gb170|OS12G41590 3291 532 82.5 globlastp 1169 LYM351barley|10v2|BF624095_T1 3292 532 81.79 glotblastn 1170 LYM351barley|gb157SOLEXA|BF624095 3292 532 81.79 glotblastn 1171 LYM351brachypodium|09v1|DV470161_T1 3293 532 81.09 glotblastn 1172 LYM351wheat|10v2|BQ578337_T1 3294 532 80.07 glotblastn 1173 LYM352maize|10v1|FK962564_T1 3295 533 98.1 glotblastn 1174 LYM352maize|10v1|ZMCRP2V098316_T1 — 533 88.61 glotblastn 1175 LYM352maize|10v1|DW790475_T1 3296 533 81.66 glotblastn 1176 LYM354sugarcane|10v1|CA071540_P1 3297 535 94.6 globlastp 1177 LYM354sugarcane|gb157.3|CA071540 3298 535 94 globlastp 1178 LYM354switchgrass|gb167|FE629774 3299 535 87.9 globlastp 1179 LYM354foxtail_millet|10v2|SICRP038756_P1 3300 535 86.2 globlastp 1180 LYM354rice|gb170|OS02G55590 3301 535 80.7 globlastp 1181 LYM356sorghum|09v1|SB04G033890 3302 537 94.7 globlastp 1182 LYM356sugarcane|10v1|CA088037_P1 3303 537 93.9 globlastp 1183 LYM356switchgrass|gb167|FE619329 3304 537 92.4 globlastp 1184 LYM356millet|10v1|EVO454PM004917_P1 3305 537 90.3 globlastp 1185 LYM356rice|gb170|OS02G52270_P1 3306 537 86.6 globlastp 1186 LYM356brachypodium|09v1|GT777127_P1 3307 537 85.9 globlastp 1187 LYM356oat|10v2|GO590102_P1 3308 537 85.1 globlastp 1188 LYM356wheat|10v2|BE398624_P1 3309 537 82.1 globlastp 1189 LYM356foxtail_millet|10v2|FXTRMSLX00005143D1_P1 3310 537 81.8 globlastp 1190LYM356 barley|10v2|BG416537_P1 3311 537 81.3 globlastp 1191 LYM356sugarcane|gb157.3|CA088037 3312 537 81.3 globlastp 1192 LYM359sorghum|09v1|SB09G020350 3313 539 89.6 globlastp 1193 LYM359switchgrass|gb167|FE598142 3314 539 88.1 globlastp 1194 LYM359foxtail_millet|10v2|FXTRMSLX00780257D2_P1 3315 539 85.4 globlastp 1195LYM359 leymus|gb166|EG387725_P1 3316 539 81.1 globlastp 1196 LYM359wheat|10v2|AL820331_P1 3317 539 80.1 globlastp 1197 LYM360sorghum|09v1|SB03G046050 3318 540 93.8 globlastp 1198 LYM360rice|gb170|OS01G72340 3319 540 82 globlastp 1199 LYM361sugarcane|10v1|CA073987_P1  541 541 100 globlastp 1200 LYM361sugarcane|gb157.3|CA073987  541 541 100 globlastp 1201 LYM361switchgrass|gb167|FE604030 3320 541 98.6 globlastp 1202 LYM361maize|gb170|LLBE224739 3321 541 98.6 globlastp 1203 LYM361maize|10v1|T69045_P1 3321 541 98.6 globlastp 1204 LYM361maize|gb170|T69045 3321 541 98.6 globlastp 1205 LYM361maize|10v1|BE051344_P1 3321 541 98.6 globlastp 1206 LYM361maize|gb170|BE051344 3321 541 98.6 globlastp 1207 LYM361switchgrass|gb167|FE607881 3320 541 98.6 globlastp 1208 LYM361sorghum|09v1|SB03G040400 3322 541 98.6 globlastp 1209 LYM361foxtail_millet|10v2|SICRP029404_P1 3323 541 97.9 globlastp 1210 LYM361millet|09v1|CD725401 3323 541 97.9 globlastp 1211 LYM361millet|10v1|CD725401_P1 3323 541 97.9 globlastp 1212 LYM361cynodon|10v1|ES292039_P1 3324 541 97.2 globlastp 1213 LYM361rice|gb170|OS01G63890 3325 541 97.2 globlastp 1214 LYM361sugarcane|10v1|CA092601_P1 3326 541 95.9 globlastp 1215 LYM361switchgrass|gb167|FL883964 3326 541 95.9 globlastp 1216 LYM361sugarcane|gb157.3|CA092601 3326 541 95.9 globlastp 1217 LYM361barley|10v2|BG300925_P1 3327 541 95.9 globlastp 1218 LYM361barley|gb157SOLEXA|BG300925 3327 541 95.9 globlastp 1219 LYM361sorghum|09v1|SB09G022770 3326 541 95.9 globlastp 1220 LYM361foxtail_millet|10v2|SICRP007700_P1 3328 541 95.2 globlastp 1221 LYM361oat|10v2|GO587638_P1 3329 541 95.2 globlastp 1222 LYM361fescue|gb161|DT681630_P1 3330 541 95.2 globlastp 1223 LYM361wheat|gb164|CA721336 3331 541 95.2 globlastp 1224 LYM361wheat|gb164|BG263183 3331 541 95.2 globlastp 1225 LYM361switchgrass|gb167|FE608157 3332 541 95.2 globlastp 1226 LYM361wheat|10v2|BF201868_P1 3331 541 95.2 globlastp 1227 LYM361cynodon|10v1|ES292016_P1 3333 541 94.5 globlastp 1228 LYM361rice|gb170|OS05G37390 3334 541 94.5 globlastp 1229 LYM361banana|10v1|FF559231_P1 3335 541 94.5 globlastp 1230 LYM361banana|gb167|FF559231 3336 541 94.5 globlastp 1231 LYM361millet|09v1|CD726270 3337 541 94.5 globlastp 1232 LYM361millet|10v1|CD726270_P1 3337 541 94.5 globlastp 1233 LYM361brachypodium|09v1|GT807282_P1 3338 541 93.8 globlastp 1234 LYM361aristolochia|10v1|SRR039082S0000613_P1 3339 541 93.1 globlastp 1235LYM361 oat|10v2|CN820723_P1 3340 541 93.1 globlastp 1236 LYM361wheat|gb164|BE414873 3341 541 93.1 globlastp 1237 LYM361oil_palm|gb166|EL686982_P1 3342 541 93.1 globlastp 1238 LYM361barley|10v2|BE603233_P1 3343 541 93.1 globlastp 1239 LYM361barley|gb157SOLEXA|BE603233 3343 541 93.1 globlastp 1240 LYM361wheat|gb164|CA640118 3341 541 93.1 globlastp 1241 LYM361wheat|10v2|BE493692_P1 3343 541 93.1 globlastp 1242 LYM361wheat|gb164|BE493692 3343 541 93.1 globlastp 1243 LYM361wheat|10v2|BE414873_P1 3341 541 93.1 globlastp 1244 LYM361pineapple|10v1|DT337088_P1 3344 541 92.4 globlastp 1245 LYM361eucalyptus|gb166|CT982737_P1 3345 541 92.4 globlastp 1246 LYM361amborella|gb166|CD483512_P1 3346 541 91 globlastp 1247 LYM361aquilegia|10v2|JGIAC018781_P1 3347 541 90.3 globlastp 1248 LYM361aristolochia|10v1|SRR039082S0449912_P1 3348 541 90.3 globlastp 1249LYM361 momordica|10v1|SRR071315S0038100_P1 3349 541 90.3 globlastp 1250LYM361 cucumber|09v1|AM728462_P1 3349 541 90.3 globlastp 1251 LYM361rice|gb170|OS12G05410 3350 541 90.3 globlastp 1252 LYM361melon|10v1|AM716068_P1 3349 541 90.3 globlastp 1253 LYM361melon|gb165|AM716068 3349 541 90.3 globlastp 1254 LYM361acacia|10v1|FS590895_P1 3351 541 89.7 globlastp 1255 LYM361oak|10v1|DN950254_P1 3352 541 89.7 globlastp 1256 LYM361sunflower|10v1|CX943795_P1 3353 541 89.7 globlastp 1257 LYM361grape|gb160|BQ798937_P1 3354 541 89.7 globlastp 1258 LYM361canola|10v1|CD822899_P1 3355 541 89.7 globlastp 1259 LYM361canola|gb161|CD822899 3355 541 89.7 globlastp 1260 LYM361cotton|10v2|SRR032367S0627871_P1 3356 541 89.7 globlastp 1261 LYM361cotton|gb164|AI729628 3356 541 89.7 globlastp 1262 LYM361b_rapa|gb162|CX266853_P1 3355 541 89.7 globlastp 1263 LYM361canola|10v1|DY017536_P1 3355 541 89.7 globlastp 1264 LYM361canola|gb161|DY017536 3355 541 89.7 globlastp 1265 LYM361apple|gb171|CN916494_P1 3357 541 89.7 globlastp 1266 LYM361sunflower|gb162|CX943795 3353 541 89.7 globlastp 1267 LYM361chestnut|gb170|SRR006295S0026079_P1 3352 541 89.7 globlastp 1268 LYM361cassava|09v1|DV451765_P1 3358 541 89.7 globlastp 1269 LYM361lotus|09v1|LLBF177618_P1 3359 541 89.7 globlastp 1270 LYM361b_oleracea|gb161|AM062209_P1 3355 541 89.7 globlastp 1271 LYM361castorbean|09v1|EV521206_P1 3360 541 89.7 globlastp 1272 LYM361b_juncea|10v2|E7FJ1I304DOLGM_P1 3361 541 89 globlastp 1273 LYM361hevea|10v1|EC600539_P1 3362 541 89 globlastp 1274 LYM361ipomoea_nil|10v1|CJ740253_P1 3363 541 89 globlastp 1275 LYM361nasturtium|10v1|SRR032558S0006072_P1 3364 541 89 globlastp 1276 LYM361strawberry|11v1|CO381831_P1 3365 541 89 globlastp 1277 LYM361strawberry|gb164|CO381831 3365 541 89 globlastp 1278 LYM361ipomoea|gb157.2|BU691892 3363 541 89 globlastp 1279 LYM361rose|gb157.2|EC588056 3366 541 89 globlastp 1280 LYM361cacao|10v1|CF974197_P1 3367 541 89 globlastp 1281 LYM361cacao|gb167|CF974197 3367 541 89 globlastp 1282 LYM361radish|gb164|EY934302 3361 541 89 globlastp 1283 LYM361prunus|10v1|BU045215_P1 3368 541 89 globlastp 1284 LYM361prunus|gb167|BU045215 3368 541 89 globlastp 1285 LYM361citrus|gb166|CK938051_P1 3369 541 89 globlastp 1286 LYM361senecio|gb170|DY659224 3370 541 89 globlastp 1287 LYM361cowpea|gb166|FG841129_P1 3371 541 89 globlastp 1288 LYM361soybean|11v1|GLYMA05G28440_P1 3371 541 89 globlastp 1289 LYM361soybean|gb168|BF177618 3371 541 89 globlastp 1290 LYM361radish|gb164|EV566892 3361 541 89 globlastp 1291 LYM361peanut|10v1|GO266374_P1 3371 541 89 globlastp 1292 LYM361peanut|gb171|ES767033 3371 541 89 globlastp 1293 LYM361soybean|11v1|GLYMA08G11450_P1 3371 541 89 globlastp 1294 LYM361soybean|gb168|BG239642 3371 541 89 globlastp 1295 LYM361liquorice|gb171|FS239962_P1 3371 541 89 globlastp 1296 LYM361brachypodium|09v1|DV469043_T1 3372 541 88.59 glotblastn 1297 LYM361aquilegia|10v2|JGIAG015311_P1 3373 541 88.3 globlastp 1298 LYM361artemisia|10v1|SRR019254S0169291_P1 3374 541 88.3 globlastp 1299 LYM361b_juncea|10v2|E6ANDIZ01DW66Q_P1 3375 541 88.3 globlastp 1300 LYM361canola|10v1|ES922658_P1 3376 541 88.3 globlastp 1301 LYM361cleome_spinosa|10v1|SRR015531S0005388_P1 3377 541 88.3 globlastp 1302LYM361 cyamopsis|10v1|EG975817_P1 3378 541 88.3 globlastp 1303 LYM361eggplant|10v1|FS019113_P1 3379 541 88.3 globlastp 1304 LYM361pigeonpea|10v1|SRR054580S0005740_P1 3380 541 88.3 globlastp 1305 LYM361salvia|10v1|CV163574_P1 3381 541 88.3 globlastp 1306 LYM361sunflower|10v1|EE623253_P1 3382 541 88.3 globlastp 1307 LYM361poppy|gb166|FE964500_P1 3383 541 88.3 globlastp 1308 LYM361monkeyflower|09v1|GO975256 3384 541 88.3 globlastp 1309 LYM361monkeyflower|09v1|DV209147 3384 541 88.3 globlastp 1310 LYM361radish|gb164|EX765001 3385 541 88.3 globlastp 1311 LYM361antirrhinum|gb166|AJ791799_P1 3386 541 88.3 globlastp 1312 LYM361canola|10v1|CD820875_P1 3387 541 88.3 globlastp 1313 LYM361canola|gb161|CD820875 3387 541 88.3 globlastp 1314 LYM361monkeyflower|09v1|GO961216 3384 541 88.3 globlastp 1315 LYM361nuphar|gb166|CD474973_P1 3388 541 88.3 globlastp 1316 LYM361petunia|gb171|CV295984_P1 3389 541 88.3 globlastp 1317 LYM361sunflower|gb162|EL432089 3382 541 88.3 globlastp 1318 LYM361coffea|10v1|DV679962_P1 3390 541 88.3 globlastp 1319 LYM361coffea|gb157.2|DV679962 3390 541 88.3 globlastp 1320 LYM361radish|gb164|EX751181 3391 541 88.3 globlastp 1321 LYM361brachypodium|09v1|DV486314_P1 3392 541 88.3 globlastp 1322 LYM361dandelion|10v1|DY826045_P1 3393 541 88.3 globlastp 1323 LYM361dandelion|gb161|DY826045 3393 541 88.3 globlastp 1324 LYM361apple|gb171|CN444255_P1 3394 541 88.3 globlastp 1325 LYM361b_rapa|gb162|CA991816_P1 3387 541 88.3 globlastp 1326 LYM361monkeyflower|10v1|DV209147_P1 3384 541 88.3 globlastp 1327 LYM361b_juncea|10v2|E6ANDIZ02GACXH_P1 3395 541 87.6 globlastp 1328 LYM361dandelion|10v1|DR400677_P1 3396 541 87.6 globlastp 1329 LYM361guizotia|10v1|GE571913_P1 3397 541 87.6 globlastp 1330 LYM361lolium|10v1|SRR029311S0010475_P1 3398 541 87.6 globlastp 1331 LYM361tragopogon|10v1|SRR020205S0252287_P1 3399 541 87.6 globlastp 1332 LYM361canola|gb161|CX280171 3400 541 87.6 globlastp 1333 LYM361medicago|09v1|DW018876_P1 3401 541 87.6 globlastp 1334 LYM361poplar|10v1|BI068981_P1 3402 541 87.6 globlastp 1335 LYM361poplar|gb170|BI068981 3402 541 87.6 globlastp 1336 LYM361poplar|10v1|AI161903_P1 3403 541 87.6 globlastp 1337 LYM361poplar|gb170|AI161903 3403 541 87.6 globlastp 1338 LYM361basilicum|10v1|DY334449_P1 3404 541 87.6 globlastp 1339 LYM361basilicum|gb157.3|DY334449 3404 541 87.6 globlastp 1340 LYM361safflower|gb162|EL372749 3399 541 87.6 globlastp 1341 LYM361catharanthus|gb166|EG555992_P1 3405 541 87.6 globlastp 1342 LYM361centaurea|gb166|EH739373_P1 3399 541 87.6 globlastp 1343 LYM361canola|10v1|DY011439_P1 3406 541 87.6 globlastp 1344 LYM361canola|gb161|EE419840 3406 541 87.6 globlastp 1345 LYM361cucumber|09v1|DN909678_P1 3407 541 87.6 globlastp 1346 LYM361cynara|gb167|GE587828_P1 3399 541 87.6 globlastp 1347 LYM361gerbera|09v1|AJ751246_P1 3399 541 87.6 globlastp 1348 LYM361radish|gb164|EV545365 3408 541 87.59 glotblastn 1349 LYM361b_juncea|10v2|E6ANDIZ01A1NHB_P1 3409 541 86.9 globlastp 1350 LYM361lettuce|10v1|DW080225_P1 3410 541 86.9 globlastp 1351 LYM361melon|10v1|VMEL01979838432456_P1 3411 541 86.9 globlastp 1352 LYM361canola|10v1|CX280171_P1 3412 541 86.9 globlastp 1353 LYM361b_oleracea|gb161|EE533984_P1 3413 541 86.9 globlastp 1354 LYM361lettuce|gb157.2|DW074112 3414 541 86.9 globlastp 1355 LYM361potato|10v1|BQ518828_P1 3415 541 86.9 globlastp 1356 LYM361potato|gb157.2|BQ518828 3415 541 86.9 globlastp 1357 LYM361artemisia|10v1|EY066674_P1 3410 541 86.9 globlastp 1358 LYM361artemisia|gb164|EY066674 3410 541 86.9 globlastp 1359 LYM361cichorium|gb171|EH682067_P1 3416 541 86.9 globlastp 1360 LYM361triphysaria|10v1|EY130493_P1 3417 541 86.9 globlastp 1361 LYM361triphysaria|gb164|EY130493 3417 541 86.9 globlastp 1362 LYM361tomato|09v1|BG131354 3415 541 86.9 globlastp 1363 LYM361lettuce|10v1|DW056441_P1 3410 541 86.9 globlastp 1364 LYM361lettuce|gb157.2|DW124838 3410 541 86.9 globlastp 1365 LYM361gerbera|09v1|AJ755101_P1 3418 541 86.9 globlastp 1366 LYM361solanum_phureja|09v1|SPHBG131354 3415 541 86.9 globlastp 1367 LYM361arabidopsis|10v1|AT4G21110_P1 3419 541 86.9 globlastp 1368 LYM361arabidopsis_lyrata|09v1|JGIAL026050_P1 3419 541 86.9 globlastp 1369LYM361 pepper|gb171|BM063341_P1 3415 541 86.9 globlastp 1370 LYM361medicago|09v1|AW127096_T1 3420 541 86.9 glotblastn 1371 LYM361rice|gb170|OS04G55280 3421 541 86.84 glotblastn 1372 LYM361guizotia|10v1|GE571769_T1 3422 541 86.21 glotblastn 1373 LYM361cichorium|gb171|EH698674_T1 3423 541 86.21 glotblastn 1374 LYM361spurge|gb161|DV132742 3424 541 86.21 glotblastn 1375 LYM361b_juncea|10v2|E6ANDIZ01C61GG1_P1 3425 541 86.2 globlastp 1376 LYM361eggplant|10v1|FS028388_P1 3426 541 86.2 globlastp 1377 LYM361podocarpus|10v1|SRR065014S0009839_P1 3427 541 86.2 globlastp 1378 LYM361tragopogon|10v1|SRR020205S0014468_P1 3428 541 86.2 globlastp 1379 LYM361lettuce|10v1|DW078223_P1 3429 541 86.2 globlastp 1380 LYM361lotus|09v1|BI420153_P1 3430 541 86.2 globlastp 1381 LYM361kiwi|gb166|FG397440_P1 3431 541 86.2 globlastp 1382 LYM361chestnut|gb170|SRR006295S0103363_P1 3432 541 86.2 globlastp 1383 LYM361peanut|10v1|GO323342_P1 3433 541 86.2 globlastp 1384 LYM361peanut|gb171|GO323342 3433 541 86.2 globlastp 1385 LYM361radish|gb164|EX907259 3434 541 86.2 globlastp 1386 LYM361radish|gb164|EV550603 3435 541 86.2 globlastp 1387 LYM361triphysaria|10v1|EY143984_P1 3436 541 86.2 globlastp 1388 LYM361triphysaria|gb164|EY143984 3436 541 86.2 globlastp 1389 LYM361oak|10v1|FP063615_P1 3437 541 85.5 globlastp 1390 LYM361pigeonpea|10v1|SRR054580S0029542_P1 3438 541 85.5 globlastp 1391 LYM361lettuce|gb157.2|DW056441 3439 541 85.5 globlastp 1392 LYM361radish|gb164|EV536001 3440 541 85.5 globlastp 1393 LYM361cassava|09v1|DV441361_P1 3441 541 85.5 globlastp 1394 LYM361b_rapa|gb162|EE520703_P1 3442 541 85.5 globlastp 1395 LYM361liriodendron|gb166|DT584581_P1 3443 541 85.5 globlastp 1396 LYM361b_juncea|10v2|E6ANDIZ01A17L4_P1 3444 541 84.8 globlastp 1397 LYM361gnetum|10v1|CB082682_P1 3445 541 84.8 globlastp 1398 LYM361sequoia|10v1|SRR065044S0037904_P1 3446 541 84.8 globlastp 1399 LYM361taxus|10v1|SRR032523S0008600_P1 3447 541 84.8 globlastp 1400 LYM361pine|gb157.2|CF670895 3448 541 84.8 globlastp 1401 LYM361pepper|gb171|GD089582_P1 3449 541 84.8 globlastp 1402 LYM361safflower|gb162|EL403744 3450 541 84.8 globlastp 1403 LYM361thellungiella|gb167|BY802080 3451 541 84.8 globlastp 1404 LYM361petunia|gb171|FN009876_P1 3452 541 84.8 globlastp 1405 LYM361cacao|10v1|CU469868_P1 3453 541 84.8 globlastp 1406 LYM361cacao|gb167|CU469868 3453 541 84.8 globlastp 1407 LYM361salvia|10v1|SRR014553S0020301_P1 3454 541 84.1 globlastp 1408 LYM361soybean|gb168|AW127096 3455 541 84.1 globlastp 1409 LYM361iceplant|gb164|CA832422_P1 3456 541 84.1 globlastp 1410 LYM361fern|gb171|DK951780_P1 3457 541 84.1 globlastp 1411 LYM361cryptomeria|gb166|BY878663_P1 3458 541 84.1 globlastp 1412 LYM361solanum_phureja|09v1|SPHBG123343 3459 541 84.1 globlastp 1413 LYM361potato|gb157.2|BG590089 3460 541 84.1 globlastp 1414 LYM361potato|10v1|BG590089_P1 3460 541 84.1 globlastp 1415 LYM361pine|10v2|BF517331_P1 3461 541 83.4 globlastp 1416 LYM361tomato|09v1|BG123343 3462 541 83.4 globlastp 1417 LYM361potato|gb157.2|CK854087 3463 541 83.4 globlastp 1418 LYM361kiwi|gb166|FG406174_P1 3464 541 83.4 globlastp 1419 LYM361ginseng|10v1|GR874863_P1 3465 541 82.8 globlastp 1420 LYM361pine|gb157.2|BF517331 3466 541 82.8 globlastp 1421 LYM361marchantia|gb166|BJ857236_P1 3467 541 82.8 globlastp 1422 LYM361citrus|gb166|CX636054_P1 3468 541 82.8 globlastp 1423 LYM361sciadopitys|10v1|SRR065035S0113890_T1 3469 541 82.76 glotblastn 1424LYM361 pseudotsuga|10v1|SRR065119S0057200_P1 3470 541 82.1 globlastp1425 LYM361 leymus|gb166|EG386976_P1 3471 541 82.1 globlastp 1426 LYM361lolium|10v1|DT670946_P1 3472 541 82.1 globlastp 1427 LYM361fern|gb171|DK956086_P1 3473 541 82.1 globlastp 1428 LYM361cleome_spinosa|10v1|SRR015531S0048103_T1 3474 541 82.07 glotblastn 1429LYM361 ceratodon|10v1|SRR074890S0014886_P1 3475 541 81.4 globlastp 1430LYM361 castorbean|09v1|XM002529032_P1 3476 541 81.4 globlastp 1431LYM361 physcomitrella|10v1|BQ040629_P1 3477 541 81.4 globlastp 1432LYM361 orobanche|10v1|SRR023189S0055567_P1 3478 541 80.7 globlastp 1433LYM361 sugarcane|10v1|CA153039_T1 3479 541 80.69 glotblastn 1434 LYM361iceplant|gb164|BE036439_T1 3480 541 80.69 glotblastn 1435 LYM361strawberry|11v1|CRPFV015322_P1 3481 541 80 globlastp 1436 LYM361physcomitrella|10v1|BJ586722_P1 3482 541 80 globlastp 1437 LYM362sorghum|09v1|SB06G027130 3483 542 91.4 globlastp 1438 LYM363sugarcane|10v1|BQ529715_P1 3484 543 95.9 globlastp 1439 LYM363sugarcane|gb157.3|BQ529715 3485 543 95.4 globlastp 1440 LYM363barley|10v2|BF626430_P1 3486 543 83.9 globlastp 1441 LYM363barley|gb157SOLEXA|BF626430 3486 543 83.9 globlastp 1442 LYM364sorghum|09v1|SB02G009450 3487 544 92.8 globlastp 1443 LYM364switchgrass|gb167|DN151397 3488 544 80 globlastp 1444 LYM365maize|10v1|BF733100_P1 3489 545 95.1 globlastp 1445 LYM365sorghum|09v1|SB04G002260 3490 545 90.9 globlastp 1446 LYM365foxtail_millet|10v2|SICRP011045_T1 3491 545 89.16 glotblastn 1447 LYM365maize|gb170|BF733100 3492 545 88.5 globlastp 1448 LYM365switchgrass|gb167|FE605040 3493 545 81.6 globlastp 1449 LYM365pseudoroegneria|gb167|FF352749 3494 545 81.4 globlastp 1450 LYM366sorghum|09v1|SB10G008220 3495 546 95.2 globlastp 1451 LYM366foxtail_millet|10v2|SICRP029763_P1 3496 546 93.6 globlastp 1452 LYM366switchgrass|gb167|FL775385 3497 546 93.6 globlastp 1453 LYM366switchgrass|gb167|FL795206 3498 546 92 globlastp 1454 LYM366millet|10v1|EVO454PM340015_P1 3499 546 92 globlastp 1455 LYM366millet|09v1|EVO454PM417719 3500 546 91.44 glotblastn 1456 LYM366sugarcane|10v1|CA293234_P1 3501 546 91.4 globlastp 1457 LYM366rice|gb170|OS06G12500 3502 546 88.8 globlastp 1458 LYM368sugarcane|gb157.3|CA080429 3503 548 90.24 glotblastn 1458 LYM368_H4sugarcane|gb157.3|CA080429 3503 679 89.9 globlastp 1459 LYM368maize|gb170|AI065874 3504 548 89.84 glotblastn 1459 LYM368_H4maize|gb170|AI065874 3504 679 91.3 globlastp 1460 LYM368maize|gb170|AI901397 3505 548 89.84 glotblastn 1460 LYM368_H4maize|gb170|AI901397 3505 679 83.8 globlastp 1461 LYM368maize|10v1|AI901397_T1 3504 548 89.84 glotblastn 1461 LYM368_H4maize|10v1|AI901397_P1 3504 679 91.3 globlastp 1462 LYM368foxtail_millet|10v2|FXTRMSLX00831270D1_T1 3506 548 82.11 glotblastn 1463LYM368 millet|09v1|EVO454PM004516 3507 548 81.3 glotblastn 1464 LYM368millet|10v1|EVO454PM004516_T1 3508 548 81.3 glotblastn 1465 LYM368maize|gb170|LLCD443651 3509 548 80.08 glotblastn 1466 LYM369maize|10v1|AI637228_P1 3510 549 91.3 globlastp 1467 LYM369maize|gb170|AI637228 3510 549 91.3 globlastp 1468 LYM369wheat|gb164|CA486301 3511 549 90.9 globlastp 1469 LYM369sorghum|09v1|SB01G038910 3511 549 90.9 globlastp 1470 LYM369foxtail_millet|10v2|OXFXTSLX00010659D1T1_P1 3512 549 90.1 globlastp 1471LYM369 switchgrass|gb167|DN151852 3513 549 88.8 globlastp 1472 LYM369cenchrus|gb166|EB653323_P1 3514 549 88.2 globlastp 1473 LYM369switchgrass|gb167|DN150334 3515 549 88 globlastp 1474 LYM369rice|gb170|OS03G17470 3516 549 85.2 globlastp 1475 LYM369oat|10v2|GO586094_P1 3517 549 83.6 globlastp 1476 LYM369lovegrass|gb167|EH190420_T1 3518 549 83.47 glotblastn 1477 LYM369barley|10v2|BE411508_P1 3519 549 82 globlastp 1478 LYM369pseudoroegneria|gb167|FF346054 3520 549 81.4 globlastp 1479 LYM369leymus|gb166|EG394008_T1 3521 549 81.4 glotblastn 1480 LYM369brachypodium|09v1|DV478072_P1 3522 549 81.1 globlastp 1481 LYM371sorghum|09v1|SB03G003745 3523 551 87.7 globlastp 1482 LYM371maize|gb170|CK145019 3524 551 86.3 globlastp 1483 LYM371switchgrass|gb167|FL781064 3525 551 81.63 glotblastn 1484 LYM373sorghum|09v1|SB03G028740 3526 553 96.9 globlastp 1485 LYM373sugarcane|10v1|CA087307_P1 3527 553 96.7 globlastp 1486 LYM373sugarcane|gb157.3|CA087307 3528 553 96.6 globlastp 1487 LYM373switchgrass|gb167|FE600362 3529 553 92.7 globlastp 1488 LYM373rice|gb170|OS01G43910 3530 553 89.9 globlastp 1489 LYM373millet|09v1|EB411080 3531 553 89.4 globlastp 1490 LYM373brachypodium|09v1|DV478255_P1 3532 553 88.8 globlastp 1491 LYM373wheat|10v2|BE405537_P1 3533 553 88.3 globlastp 1492 LYM373wheat|gb164|BE405537 3534 553 88.1 globlastp 1493 LYM373barley|10v2|AV836431_P1 3535 553 87.2 globlastp 1494 LYM373fescue|gb161|DT687544_P1 3536 553 83.1 globlastp 1495 LYM374maize|10v1|EE187960_P1 3537 554 98.3 globlastp 1496 LYM374maize|gb170|EE187960 3538 554 93.6 globlastp 1497 LYM374maize|10v1|AI855357_P1 3539 554 86 globlastp 1498 LYM374sorghum|09v1|SB07G024770 3540 554 85.3 globlastp 1499 LYM376sugarcane|10v1|CA102891_P1 3541 556 97.5 globlastp 1500 LYM376sugarcane|gb157.3|CA102891 3541 556 97.5 globlastp 1501 LYM376foxtail_millet|10v2|SICRP002879_P1 3542 556 95.8 globlastp 1502 LYM376sorghum|09v1|SB06G020530 3543 556 95.8 globlastp 1503 LYM376millet|10v1|PMSLX0036334D1_P1 3544 556 94.3 globlastp 1504 LYM376switchgrass|gb167|FE638189 3545 556 94.1 globlastp 1505 LYM376oat|10v2|GR318581_P1 3546 556 89.1 globlastp 1506 LYM376oat|10v2|GR341075_P1 3546 556 89.1 globlastp 1507 LYM376wheat|gb164|CA742260 3547 556 89.1 globlastp 1508 LYM376wheat|gb164|BE443106 3548 556 89.1 globlastp 1509 LYM376wheat|10v2|BE443106_P1 3547 556 89.1 globlastp 1510 LYM376barley|10v2|BI951581_P1 3549 556 88.2 globlastp 1511 LYM376barley|gb157SOLEXA|BI951581 3549 556 88.2 globlastp 1512 LYM376brachypodium|09v1|GT770899_P1 3550 556 87.6 globlastp 1513 LYM376wheat|gb164|CA690234 3551 556 87.4 globlastp 1514 LYM376rice|gb170|OS04G40670 3552 556 84.8 globlastp 1515 LYM376fescue|gb161|DT691534_P1 3553 556 84 globlastp 1516 LYM382sugarcane|gb157.3|CA089412 3554 562 94.7 globlastp 1517 LYM382maize|10v1|BE025386_P1 3555 562 94.5 globlastp 1518 LYM382maize|gb170|BE025386 3555 562 94.5 globlastp 1519 LYM382sorghum|09v1|SB09G005480 3556 562 90.4 globlastp 1520 LYM382switchgrass|gb167|FE625547 3557 562 89.2 globlastp 1521 LYM382brachypodium|09v1|DV477955_P1 3558 562 84.9 globlastp 1522 LYM382oat|10v2|GR317157_P1 3559 562 83.6 globlastp 1523 LYM382barley|10v2|BF261359_P1 3560 562 81.7 globlastp 1524 LYM382rice|gb170|OS05G08640 3561 562 80.9 globlastp 1525 LYM385maize|10v1|ZMCRP2V006931_T1 3562 565 97.32 glotblastn 1526 LYM385maize|10v1|ZMCRP2V101351_T1 3563 565 95.79 glotblastn 1527 LYM385maize|10v1|ZMCRP2V103873_T1 3564 565 94.64 glotblastn 1528 LYM385maize|10v1|ZMCRP2V150534_T1 3565 565 94.64 glotblastn 1529 LYM385maize|10v1|EG106499_T1 3566 565 93.49 glotblastn 1530 LYM385maize|10v1|ZMCRP2V072664_P1 3567 565 93.1 globlastp 1531 LYM385maize|10v1|ZMCRP2V158795_T1 3568 565 92.72 glotblastn 1532 LYM385maize|10v1|ZMCRP2V052949_P1 3569 565 92.7 globlastp 1533 LYM385maize|10v1|ZMCRP2V198465_P1 3570 565 92.7 globlastp 1534 LYM385maize|10v1|ZMCRP2V062992_T1 3571 565 92.34 glotblastn 1535 LYM385maize|10v1|ZMCRP2V009897_P1 3572 565 92.3 globlastp 1536 LYM385maize|10v1|ZMCRP2V013149_P1 3573 565 92 globlastp 1537 LYM385maize|10v1|ZMCRP2V220907_T1 3574 565 91.19 glotblastn 1538 LYM385maize|10v1|ZMCRP2V036361_P1 3575 565 90.8 globlastp 1539 LYM385maize|10v1|ZMCRP2V173171_T1 3576 565 89.66 glotblastn 1540 LYM385maize|10v1|ZMCRP2V110808_P1 3577 565 87.7 globlastp 1541 LYM385maize|10v1|SRR014552S0020338_P1 3578 565 85.8 globlastp 1542 LYM385maize|gb170|LLBI389401 3579 565 84.7 globlastp 1543 LYM385maize|10v1|ZMCRP2V206060_T1 3580 565 84.29 glotblastn 1544 LYM385maize|10v1|EU961782_P1 3581 565 83.5 globlastp 1545 LYM385maize|gb170|EU961782 3582 565 82 globlastp 1546 LYM387sorghum|09v1|SB01G011750 3583 567 80 globlastp 1547 LYM388sugarcane|gb157.3|CA076939 3584 568 98.6 globlastp 1548 LYM388sorghum|09v1|SB10G009560 3585 568 98 globlastp 1549 LYM388foxtail_millet|10v2|OXFXTSLX00018958D1T1_P1 3586 568 95.3 globlastp 1550LYM388 millet|10v1|EVO454PM004255_P1 3587 568 95.3 globlastp 1551 LYM388switchgrass|gb167|DN144787 3588 568 93.9 globlastp 1552 LYM388switchgrass|gb167|DN145508 3589 568 92.7 globlastp 1553 LYM388switchgrass|gb167|FL840870 3590 568 92 globlastp 1554 LYM388cynodon|10v1|ES292609_P1 3591 568 89.9 globlastp 1555 LYM388lovegrass|gb167|DN480848_P1 3592 568 88.7 globlastp 1556 LYM388maize|gb170|AW438322 3593 568 88.3 globlastp 1557 LYM388maize|10v1|AW438322_P1 3593 568 88.3 globlastp 1558 LYM388brachypodium|09v1|DV475893_P1 3594 568 85.7 globlastp 1559 LYM388maize|gb170|LLEE031732 3595 568 85.6 globlastp 1560 LYM388leymus|gb166|CD808936_P1 3596 568 84.4 globlastp 1561 LYM388foxtail_millet|10v2|FXTSLX00025055_P1 3597 568 83.8 globlastp 1562LYM388 wheat|10v2|BG274116_P1 3598 568 83.8 globlastp 1563 LYM388wheat|10v2|BQ789371_P1 3597 568 83.8 globlastp 1564 LYM388pseudoroegneria|gb167|FF342073 3599 568 83.8 globlastp 1565 LYM388rice|gb170|OS06G15400 3600 568 83.3 globlastp 1566 LYM388oat|10v2|CN818325_P1 3601 568 83.2 globlastp 1567 LYM388wheat|10v2|BF293736_T1 3602 568 83.12 glotblastn 1568 LYM388wheat|10v2|BE418483_P1 3603 568 83.1 globlastp 1569 LYM388fescue|gb161|DT680555_P1 3604 568 83.1 globlastp 1570 LYM388barley|10v2|BE420957XX2_P1 3605 568 83.1 globlastp 1571 LYM388barley|gb157SOLEXA|AL450585 3605 568 83.1 globlastp 1572 LYM388lolium|09v1|AU246422 3606 568 83.1 globlastp 1573 LYM388lolium|10v1|AU246422_P1 3606 568 83.1 globlastp 1574 LYM388wheat|10v2|CA597940_T1 3607 568 82.47 glotblastn 1575 LYM388rye|gb164|BE704519 3608 568 82.47 glotblastn 1576 LYM388maize|10v1|W59830_P1 3609 568 80.9 globlastp 1576 LYM476maize|10v1|W59830_P1 3609 642 83.6 globlastp 1577 LYM388maize|gb170|W59830 3609 568 80.9 globlastp 1577 LYM476maize|gb170|W59830 3609 642 83.6 globlastp 1578 LYM392brachypodium|09v1|GT772123_P1 3610 572 82.3 globlastp 1579 LYM392sorghum|09v1|SB03G027850 3611 572 81.61 glotblastn 1580 LYM392maize|10v1|BM381239_P1 3612 572 81.2 globlastp 1581 LYM392maize|gb170|BM381239 3612 572 81.2 globlastp 1582 LYM392sugarcane|10v1|BQ537130_P1 3613 572 81 globlastp 1583 LYM392oat|10v2|GR314082_P1 3614 572 80.9 globlastp 1584 LYM392wheat|10v2|BI480480_T1 3615 572 80.04 glotblastn 1585 LYM393sugarcane|10v1|BQ537441_P1 3616 573 83.9 globlastp 1586 LYM393sugarcane|gb157.3|BQ534913 3616 573 83.9 globlastp 1587 LYM393sorghum|09v1|SB03G029290 3616 573 83.9 Globlastp 1588 LYM393sugarcane|gb157.3|BQ534082 3616 573 83.9 Globlastp 1589 LYM393sugarcane|10v1|BQ534082_P1 3616 573 83.9 Globlastp 1590 LYM393switchgrass|gb167|DN145383 3617 573 83.6 Globlastp 1591 LYM393switchgrass|gb167|GD008504 3618 573 83.6 Globlastp 1592 LYM393zostera|10v1|SRR057351S0259397_T1 3619 573 81.97 Glotblastn 1593 LYM393lovegrass|gb167|DN480320_T1 3620 573 81.97 Glotblastn 1594 LYM393switchgrass|gb167|FL843312 3621 573 80.3 Globlastp 1595 LYM393millet|09v1|EVO454PM039216 — 573 80 Glotblastn 1596 LYM395brachypodium|09v1|DV479992_P1 3622 575 86.5 Globlastp 1597 LYM395pseudoroegneria|gb167|FF343684 3623 575 86.5 Globlastp 1598 LYM395barley|10v2|BG300466_P1 3624 575 86 globlastp 1599 LYM395barley|gb157SOLEXA|BG300466 3624 575 86 Globlastp 1600 LYM395leymus|gb166|EG378693_P1 3625 575 86 Globlastp 1601 LYM395sorghum|09v1|SB04G002070 3626 575 84.8 Globlastp 1602 LYM395sugarcane|gb157.3|CA071035 3627 575 84.6 Globlastp 1603 LYM395sugarcane|10v1|CA071035_P1 3628 575 84.4 Globlastp 1604 LYM395switchgrass|gb167|DN144256 3629 575 84 Globlastp 1605 LYM395switchgrass|gb167|DN145741 3630 575 83.5 Globlastp 1606 LYM395maize|10v1|AA011879_P1 3631 575 83.4 Globlastp 1607 LYM395maize|gb170|AA011879 3632 575 83 Globlastp 1608 LYM395foxtail_millet|10v2|SICRP030709_P1 3633 575 82.6 Globlastp 1609 LYM397brachypodium|09v1|GT779489_P1 3634 577 82 Globlastp 1609 LYM397_H2brachypodium|09v1|GT779489_T1 3634 680 81.27 Glotblastn 1610 LYM398brachypodium|09v1|DV472507_P1 3635 578 81.8 Globlastp 1611 LYM398switchgrass|gb167|DN141900 3636 578 80.7 Globlastp 1612 LYM400brachypodium|09v1|GT776786_P1 3637 580 88.8 Globlastp 1613 LYM400sorghum|09v1|SB01G040970 3638 580 88.4 Globlastp 1614 LYM400wheat|10v2|BE516698_P1 3639 580 87.7 Globlastp 1615 LYM400wheat|gb164|BE516698 3640 580 87.5 Globlastp 1616 LYM400maize|10v1|BE924837_P1 3641 580 87.5 Globlastp 1617 LYM400maize|gb170|BE924837 3642 580 87.47 Glotblastn 1618 LYM400barley|10v2|BE438908_P1 3643 580 87.3 Globlastp 1619 LYM400foxtail_millet|10v2|OXEC612720T1_P1 3644 580 86.3 Globlastp 1620 LYM400oat|10v2|CN815176_P1 3645 580 85.6 Globlastp 1621 LYM400rice|gb170|OS10G10500 3646 580 84.5 Globlastp 1622 LYM400brachypodium|09v1|SRR031797S0365169_P1 3647 580 80.9 Globlastp 1623LYM400 millet|10v1|EVO454PM000568_P1 3648 580 80.4 Globlastp 1624 LYM400maize|10v1|AW017832_P1 3649 580 80 globlastp 1625 LYM400maize|gb170|AW017832 3649 580 80 Globlastp 1626 LYM402brachypodium|09v1|GT813612_P1 3650 582 88.1 Globlastp 1627 LYM402sorghum|09v1|SB01G008180 3651 582 80.2 Globlastp 1628 LYM404rice|gb170|OS04G54300 3652 584 92 Globlastp 1629 LYM404rice|gb170|OS04G54310 3653 584 90.7 Glotblastn 1630 LYM404fescue|gb161|DT709832_P1 3654 584 90 Globlastp 1631 LYM404wheat|gb164|AL819712 3655 584 90 Globlastp 1632 LYM404wheat|10v2|AL819712_P1 3655 584 90 Globlastp 1633 LYM404wheat|gb164|CD915389 3656 584 88.9 Globlastp 1634 LYM404wheat|10v2|CJ538972_P1 3657 584 88.6 Globlastp 1635 LYM404wheat|10v2|CD907992_P1 3658 584 88.6 Globlastp 1636 LYM404wheat|gb164|CD907992 3659 584 88.6 Globlastp 1637 LYM404wheat|10v2|CA615634_T1 3660 584 88.51 Glotblastn 1638 LYM404wheat|gb164|CA615634 3660 584 88.51 Glotblastn 1639 LYM404brachypodium|09v1|DV468904_T1 3661 584 88.37 Glotblastn 1640 LYM404wheat|gb164|BE424453 3662 584 87.9 Globlastp 1641 LYM404wheat|10v2|BE517528_P1 3663 584 87.8 Globlastp 1642 LYM404wheat|gb164|BE517528 3663 584 87.8 Globlastp 1643 LYM404wheat|gb164|BE419640 3664 584 87.5 Glotblastn 1644 LYM404wheat|10v2|BE419640_T1 — 584 87.5 Glotblastn 1645 LYM404oat|10v2|GR357592_P1 3665 584 86.8 Globlastp 1646 LYM404wheat|10v2|CA646285_P1 3666 584 86.7 Globlastp 1647 LYM404wheat|10v2|BE425044_P1 3667 584 86.7 Globlastp 1648 LYM404wheat|gb164|BE425044 3667 584 86.7 Globlastp 1649 LYM404wheat|gb164|AL809396 3668 584 86.7 Globlastp 1650 LYM404pseudoroegneria|gb167|FF354284 3669 584 86.7 Globlastp 1651 LYM404wheat|gb164|CA646285 3666 584 86.7 Globlastp 1652 LYM404wheat|10v2|BQ802698_P1 3670 584 86.4 Globlastp 1653 LYM404wheat|10v2|BG606870_T1 3671 584 86.36 Glotblastn 1654 LYM404wheat|gb164|BG606870 3671 584 86.36 Glotblastn 1655 LYM404wheat|gb164|BQ802698 3672 584 86.36 Glotblastn 1656 LYM404wheat|10v2|AL819097_P1 3673 584 85.6 Globlastp 1657 LYM404wheat|gb164|CA615057 3674 584 85.6 Globlastp 1658 LYM404wheat|10v2|CD905343_P1 3675 584 85.6 Globlastp 1659 LYM404wheat|gb164|CD905343 3675 584 85.6 globlastp 1660 LYM404maize|gb170|LLDQ245819 3673 584 85.6 Globlastp 1661 LYM404barley|gb157SOLEXA|BG344791 3676 584 85.6 Globlastp 1662 LYM404wheat|gb164|AL819097 3673 584 85.6 Globlastp 1663 LYM404wheat|gb164|CJ775901 3677 584 85.6 Globlastp 1664 LYM404wheat|10v2|CA730798_P1 3678 584 85.2 Globlastp 1665 LYM404wheat|gb164|CA730798 3678 584 85.2 Globlastp 1666 LYM404wheat|10v2|CA644742_P1 3679 584 85.2 Globlastp 1667 LYM404wheat|gb164|CA644742 3679 584 85.2 Globlastp 1668 LYM404barley|10v2|AJ434840_P1 3680 584 84.6 Globlastp 1669 LYM404barley|gb157SOLEXA|AJ434840 3680 584 84.6 Globlastp 1670 LYM404barley|gb157SOLEXA|AL502808 3681 584 84.4 Globlastp 1671 LYM404lolium|10v1|AU245769_P1 3682 584 84.4 Globlastp 1672 LYM404wheat|gb164|BM136835 3683 584 84.4 Globlastp 1673 LYM404barley|10v2|AJ434835_P1 3684 584 84.4 Globlastp 1674 LYM404barley|gb157SOLEXA|AL507407 3684 584 84.4 Globlastp 1675 LYM404barley|10v2|BG344791_P1 3681 584 84.4 Globlastp 1676 LYM404maize|gb170|LLDQ246118 3685 584 84.1 Globlastp 1677 LYM404wheat|10v2|CJ630414_P1 3686 584 84.1 Globlastp 1678 LYM404sorghum|09v1|SB06G029900 3687 584 83.9 Globlastp 1679 LYM404wheat|10v2|CA608688_P1 3688 584 83.7 Globlastp 1680 LYM404wheat|gb164|CA608688 3688 584 83.7 Globlastp 1681 LYM404wheat|gb164|BE429674 3689 584 83.33 Glotblastn 1682 LYM404wheat|10v2|BQ802339_P1 3690 584 83.3 Globlastp 1683 LYM404wheat|gb164|BQ578897 3691 584 83.3 Globlastp 1684 LYM404wheat|gb164|BJ276368 3692 584 83.3 Globlastp 1685 LYM404barley|10v2|AJ473977_P1 3693 584 83.3 Globlastp 1686 LYM404barley|gb157SOLEXA|AJ473977 3693 584 83.3 Globlastp 1687 LYM404wheat|gb164|CJ835344 3694 584 83 Globlastp 1688 LYM404wheat|gb164|CJ630414 3695 584 83 Globlastp 1689 LYM404wheat|10v2|CA614688_P1 3696 584 83 Globlastp 1690 LYM404wheat|gb164|CA614688 3696 584 83 Globlastp 1691 LYM404wheat|10v2|CA603078_T1 3697 584 82.95 glotblastn 1692 LYM404wheat|gb164|CA603078 3697 584 82.95 Glotblastn 1693 LYM404foxtail_millet|10v2|FXTRMSLX02628570D1_P1 3698 584 82.6 Globlastp 1694LYM404 millet|10v1|PMSLX0019838D2_P1 3699 584 82.6 Globlastp 1695 LYM404wheat|10v2|CA716773_P1 3700 584 82.6 Globlastp 1696 LYM404wheat|gb164|CA716773 3700 584 82.6 Globlastp 1697 LYM404wheat|10v2|BU099355_P1 3701 584 82.6 Globlastp 1698 LYM404wheat|gb164|BU099355 3701 584 82.6 Globlastp 1699 LYM404maize|gb170|LLDQ244531 3700 584 82.6 Globlastp 1700 LYM404oat|10v2|GO596539_P1 3702 584 82.4 Globlastp 1701 LYM404wheat|10v2|CA602736_P1 3703 584 82.4 Globlastp 1702 LYM404wheat|gb164|CA602736 3703 584 82.4 Globlastp 1703 LYM404oat|10v2|GO584079_P1 3704 584 82.2 Globlastp 1704 LYM404wheat|10v2|CJ952645_P1 3705 584 82.2 Globlastp 1705 LYM404wheat|gb164|CJ952645 3705 584 82.2 Globlastp 1706 LYM404barley|gb157SOLEXA|BQ467210 3706 584 82.2 Globlastp 1707 LYM404barley|10v2|AJ473476_P1 3707 584 81.8 Globlastp 1708 LYM404barley|gb157SOLEXA|AJ473476 3707 584 81.8 Globlastp 1709 LYM404wheat|gb164|AL819290 3708 584 81.8 Globlastp 1710 LYM404switchgrass|gb167|FE599818 3709 584 81.4 Globlastp 1711 LYM404millet|09v1|EB411010 3710 584 81.4 Globlastp 1712 LYM404millet|10v1|EB411010_P1 3711 584 81.4 Globlastp 1713 LYM404wheat|10v2|BE402489_T1 3712 584 81.11 Glotblastn 1714 LYM404wheat|gb164|BE402489 3712 584 81.11 Glotblastn 1715 LYM404barley|10v2|BQ467210_P1 3713 584 81.1 Globlastp 1716 LYM404barley|10v2|BLYB_P1 3714 584 81.1 Globlastp 1717 LYM404barley|gb157SOLEXA|BG299705 3714 584 81.1 Globlastp 1718 LYM404foxtail_millet|10v2|FXTRMSLX01003033D1_P1 3715 584 80.2 Globlastp 1719LYM404 sugarcane|10v1|CA123349_P1 3716 584 80.2 Globlastp 1720 LYM404switchgrass|gb167|FE598208 3717 584 80.2 Globlastp 1721 LYM404sorghum|09v1|SB06G029870 3718 584 80.2 Globlastp 1722 LYM404sugarcane|gb157.3|CA118620 3719 584 80.2 globlastp 1723 LYM404barley|10v2|AJ462592_P1 3720 584 80 Globlastp 1724 LYM404wheat|10v2|BM137753_P1 3721 584 80 Globlastp 1725 LYM404wheat|10v2|CA608848_P1 3722 584 80 Globlastp 1726 LYM406rice|gb170|CV721513 3723 586 86.38 Glotblastn 1727 LYM407rice|gb170|OS05G05670 3724 587 94.87 Glotblastn 1728 LYM407barley|gb157SOLEXA|AL499770 3725 587 87.1 Globlastp 1729 LYM407wheat|10v2|BE426383_P1 3726 587 87.1 Globlastp 1730 LYM407wheat|gb164|BE426383 3726 587 87.1 Globlastp 1731 LYM407leymus|gb166|CD808664_P1 3727 587 87.1 Globlastp 1732 LYM407wheat|10v2|BE401493_P1 3728 587 86.8 Globlastp 1733 LYM407wheat|gb164|AL825859 3728 587 86.8 Globlastp 1734 LYM407wheat|gb164|CA682233 3729 587 86.5 Globlastp 1735 LYM407wheat|gb164|BF428684 3730 587 86.5 Globlastp 1736 LYM407leymus|gb166|EG375196_P1 3731 587 86.5 Globlastp 1737 LYM407barley|10v2|BE437955_P1 3732 587 86.5 Globlastp 1738 LYM407wheat|10v2|BF428684_P1 3730 587 86.5 Globlastp 1739 LYM407wheat|10v2|BF428537_P1 3733 587 86.1 Globlastp 1740 LYM407wheat|10v2|CA662087_P1 3733 587 86.1 Globlastp 1741 LYM407pseudoroegneria|gb167|FF341565 3734 587 86.1 Globlastp 1742 LYM407wheat|10v2|BE417991_P1 3735 587 86.1 Globlastp 1743 LYM407wheat|gb164|BE417991 3735 587 86.1 Globlastp 1744 LYM407barley|gb157SOLEXA|BE437955 3736 587 85.8 Globlastp 1745 LYM407oat|10v2|AB128047_P1 3737 587 84.7 Globlastp 1746 LYM407oat|10v2|GR313748_P1 3738 587 84.7 Globlastp 1747 LYM407oat|10v2|GR324586_P1 3739 587 84.7 Globlastp 1748 LYM407brachypodium|09v1|DV473156_P1 3740 587 84.3 Globlastp 1749 LYM407oat|10v2|GR325179_P1 3741 587 84.1 Globlastp 1750 LYM407brachypodium|09v1|DV473203_P1 3742 587 84 Globlastp 1751 LYM407sorghum|09v1|SB09G003800 3743 587 80.7 Globlastp 1752 LYM407maize|10v1|BG836303_T1 3744 587 80.51 Glotblastn 1753 LYM407maize|gb170|BG836303 3744 587 80.51 Glotblastn 1754 LYM407sorghum|09v1|SB09G003790 3745 587 80.19 glotblastn 1755 LYM407maize|10v1|BM080388_P1 3746 587 80.1 Globlastp 1756 LYM407maize|gb170|BM080388 3746 587 80.1 Globlastp 1757 LYM409wheat|gb164|BE428448 3747 589 81.75 Glotblastn 1758 LYM410maize|gb170|AI855346 3748 590 86.7 Globlastp 1759 LYM410maize|gb170|CD946231 3749 590 85.84 Glotblastn 1760 LYM410sorghum|09v1|SB04G035150 3750 590 83.49 Glotblastn 1761 LYM415sorghum|09v1|SB02G028180 3751 595 88.3 Globlastp 1762 LYM415brachypodium|09v1|DV477194_P1 3752 595 87 Globlastp 1763 LYM415millet|10v1|PMSLX0031089D2_T1 3753 595 86.29 Glotblastn 1764 LYM415maize|10v1|BM337874_P1 3754 595 85.3 Globlastp 1765 LYM415maize|gb170|BM337874 3754 595 85.3 Globlastp 1766 LYM416brachypodium|09v1|DV470050_P1 3755 596 85.7 Globlastp 1767 LYM416switchgrass|gb167|FL829674 3756 596 85.5 Globlastp 1768 LYM416wheat|10v2|BE413636_P1 3757 596 84.3 Globlastp 1769 LYM416barley|10v2|AV916358_P1 3758 596 82.8 Globlastp 1770 LYM416barley|gb157SOLEXA|AV916358 3758 596 82.8 Globlastp 1771 LYM416sorghum|09v1|SB01G022060 3759 596 82.67 Glotblastn 1772 LYM416maize|10v1|BM267353_T1 3760 596 80.4 Glotblastn 1773 LYM416maize|gb170|BM267353 3761 596 80.4 Glotblastn 1774 LYM417switchgrass|gb167|FE605074_T1 3762 597 80.45 Glotblastn 1775 LYM418rice|gb170|OS12G07980 3763 598 97.3 Globlastp 1776 LYM418oat|gb164|CN818423 3764 598 95.95 Glotblastn 1777 LYM418oat|10v2|GO585999_P1 3765 598 94.6 Globlastp 1778 LYM418oat|10v2|CN818423_P1 3765 598 94.6 Globlastp 1779 LYM418cotton|gb164|BG443918 3766 598 94.59 Glotblastn 1780 LYM418pineapple|gb157.2|CO731527 3767 598 93.33 Glotblastn 1780 LYM418pineapple|10v1|CO731527_P1 3782 598 91.9 Globlastp 1781 LYM418wheat|gb164|CA605578 3768 598 93.24 Glotblastn 1782 LYM418wheat|gb164|CA742116 3769 598 93.24 Glotblastn 1783 LYM418wheat|gb164|BE445451 3770 598 93.24 Glotblastn 1784 LYM418wheat|10v2|BE445451_P1 3771 598 93.2 globlastp 1785 LYM418leymus|gb166|EG392853_P1 3771 598 93.2 Globlastp 1786 LYM418pseudoroegneria|gb167|FF359248 3771 598 93.2 Globlastp 1787 LYM418brachypodium|09v1|GT762052_P1 3772 598 93.2 Globlastp 1788 LYM418millet|09v1|EVO454PM003526 3773 598 93.2 Globlastp 1789 LYM418millet|10v1|EVO454PM003526_P1 3773 598 93.2 Globlastp 1790 LYM418wheat|10v2|CD927277_P1 3771 598 93.2 Globlastp 1791 LYM418wheat|gb164|CD927277 3771 598 93.2 Globlastp 1792 LYM418wheat|10v2|BE471110_P1 3774 598 93.2 Globlastp 1793 LYM418wheat|gb164|BE471110 3774 598 93.2 Globlastp 1794 LYM418lolium|09v1|AU245988 3775 598 93.2 Globlastp 1795 LYM418lolium|10v1|AU245988_P1 3775 598 93.2 Globlastp 1796 LYM418barley|10v2|BE601861_P1 3771 598 93.2 Globlastp 1797 LYM418barley|gb157SOLEXA|BE601861 3771 598 93.2 Globlastp 1798 LYM418fescue|gb161|DT688465_P1 3771 598 93.2 Globlastp 1799 LYM418wheat|10v2|BQ903037_P1 3771 598 93.2 Globlastp 1800 LYM418wheat|gb164|BQ903037 3771 598 93.2 Globlastp 1801 LYM418barley|10v2|BF257863_P1 3771 598 93.2 Globlastp 1802 LYM418barley|gb157SOLEXA|AL506323 3771 598 93.2 Globlastp 1803 LYM418wheat|10v2|CA605578_P1 3771 598 93.2 Globlastp 1804 LYM418aristolochia|10v1|SRR039086S0073523_P1 3776 598 91.9 Globlastp 1805LYM418 cacao|10v1|CU481369_P1 3777 598 91.9 Globlastp 1806 LYM418curcuma|10v1|DY385628_P1 3778 598 91.9 Globlastp 1807 LYM418cynodon|10v1|ES296934_P1 3779 598 91.9 Globlastp 1808 LYM418foxtail_millet|10v2|OXFXTSLX00015150D1T1_P1 3780 598 91.9 Globlastp 1809LYM418 heritiera|10v1|SRR005794S0001491_P1 3781 598 91.9 Globlastp 1810LYM418 cotton|10v2|AJ513288_P1 3777 598 91.9 Globlastp 1811 LYM418cotton|gb164|AJ513288 3777 598 91.9 Globlastp 1812 LYM418cacao|10v1|CA795814_P1 3777 598 91.9 Globlastp 1813 LYM418cacao|gb167|CA795814 3777 598 91.9 globlastp 1814 LYM418grape|gb160|BQ793781_P1 3777 598 91.9 Globlastp 1815 LYM418cotton|gb164|AI729188 3777 598 91.9 Globlastp 1816 LYM418switchgrass|gb167|FE599523 3783 598 91.9 Globlastp 1817 LYM418banana|10v1|FL659758_P1 3784 598 91.9 Globlastp 1818 LYM418banana|gb167|FL659758 3784 598 91.9 Globlastp 1819 LYM418switchgrass|gb167|DN150897 3783 598 91.9 Globlastp 1820 LYM418safflower|gb162|EL374434 3785 598 91.89 Glotblastn 1821 LYM418ginger|gb164|DY367049_T1 3786 598 91.89 Glotblastn 1822 LYM418ipomoea|gb157.2|EE880087 3787 598 91.89 Glotblastn 1823 LYM418oil_palm|gb166|EL690696_T1 3788 598 90.54 Glotblastn 1824 LYM418ginger|gb164|DY345687_T1 3789 598 90.54 Glotblastn 1825 LYM418sunflower|gb162|CD853045 3790 598 90.54 Glotblastn 1826 LYM418ipomoea_batatas|10v1|EE880087_P1 3791 598 90.5 Globlastp 1827 LYM418ipomoea_nil|10v1|CJ739709_P1 3791 598 90.5 Globlastp 1828 LYM418nasturtium|10v1|GH166341_P1 3792 598 90.5 Globlastp 1829 LYM418nasturtium|10v1|SRR032558S0062783_P1 3792 598 90.5 Globlastp 1830 LYM418pine|10v2|AA739786_P1 3793 598 90.5 Globlastp 1831 LYM418pine|10v2|BX253951_P1 3793 598 90.5 Globlastp 1832 LYM418prunus|10v1|CN494842_P1 3794 598 90.5 Globlastp 1833 LYM418triphysaria|10v1|SRR023501S0042197_P1 3795 598 90.5 Globlastp 1834LYM418 zostera|10v1|AM771009_P1 3796 598 90.5 Globlastp 1835 LYM418amborella|gb166|CK766571_P1 3797 598 90.5 Globlastp 1836 LYM418spruce|gb162|CO215259 3798 598 90.5 Globlastp 1837 LYM418spruce|gb162|CO216844 3798 598 90.5 Globlastp 1838 LYM418poplar|10v1|AI165261_P1 3795 598 90.5 Globlastp 1839 LYM418poplar|gb170|AI165261 3795 598 90.5 Globlastp 1840 LYM418maize|10v1|AI621467_P1 3799 598 90.5 Globlastp 1841 LYM418maize|gb170|AI621467 3799 598 90.5 Globlastp 1842 LYM418sugarcane|gb157.3|CA077022 3799 598 90.5 globlastp 1843 LYM418pine|gb157.2|BX250295 3793 598 90.5 Globlastp 1844 LYM418sorghum|09v1|SB08G004910 3799 598 90.5 Globlastp 1845 LYM418banana|10v1|GFXAC186754X43_P1 3800 598 90.5 Globlastp 1846 LYM418banana|gb167|FF557988 3800 598 90.5 Globlastp 1847 LYM418catharanthus|gb166|EG561722_P1 3801 598 90.5 Globlastp 1848 LYM418pine|gb157.2|BG275115 3793 598 90.5 Globlastp 1849 LYM418poplar|10v1|BU822258_P1 3795 598 90.5 Globlastp 1850 LYM418poplar|gb170|BU822258 3795 598 90.5 Globlastp 1851 LYM418senecio|gb170|SRR006592S0000348 3802 598 90.5 Globlastp 1852 LYM418maize|10v1|AI901423_P1 3799 598 90.5 Globlastp 1853 LYM418sugarcane|gb157.3|BQ536239 3799 598 90.5 Globlastp 1854 LYM418ipomoea|gb157.2|CJ739709 3791 598 90.5 Globlastp 1855 LYM418coffea|10v1|DV684513_P1 3795 598 90.5 Globlastp 1856 LYM418coffea|gb157.2|DV684513 3795 598 90.5 Globlastp 1857 LYM418centaurea|gb166|EH737458_P1 3802 598 90.5 Globlastp 1858 LYM418pine|gb157.2|AA739786 3793 598 90.5 Globlastp 1859 LYM418tea|10v1|FE861249_P1 3795 598 90.5 Globlastp 1860 LYM418sugarcane|10v1|BQ536239_P1 3799 598 90.5 Globlastp 1861 LYM418artemisia|10v1|SRR019254S0058219_P1 3803 598 89.2 Globlastp 1862 LYM418dandelion|10v1|DY811211_P1 3803 598 89.2 Globlastp 1863 LYM418ipomoea_nil|10v1|BJ557301_P1 3804 598 89.2 Globlastp 1863 LYM418ipomoea|gb157.2|BJ557301 3804 598 89.2 Globlastp 1864 LYM418orobanche|10v1|SRR023189S0002300_P1 3805 598 89.2 Globlastp 1865 LYM418pseudotsuga|10v1|SRR065119S0000333_P1 3806 598 89.2 Globlastp 1866LYM418 tragopogon|10v1|SRR020205S0001890_P1 3807 598 89.2 Globlastp 1867LYM418 sunflower|10v1|CD853045_P1 3803 598 89.2 Globlastp 1868 LYM418dandelion|gb161|DY811211 3803 598 89.2 globlastp 1869 LYM418lettuce|10v1|DW050308_P1 3807 598 89.2 Globlastp 1870 LYM418lettuce|gb157.2|DW050308 3807 598 89.2 Globlastp 1871 LYM418senecio|gb170|DY665725 3803 598 89.2 Globlastp 1872 LYM418oil_palm|gb166|EL692702_P1 3808 598 89.2 Globlastp 1873 LYM418beet|gb162|BI643320_P1 3809 598 89.2 Globlastp 1874 LYM418cycas|gb166|CB089512_P1 3810 598 89.2 Globlastp 1875 LYM418flax|09v1|EH792185_P1 3811 598 89.2 Globlastp 1876 LYM418apple|gb171|CN495618_P1 3812 598 89.2 Globlastp 1877 LYM418kiwi|gb166|FG414750_P1 3813 598 89.2 Globlastp 1878 LYM418nicotiana_benthamiana|gb162|ES887115_P1 3814 598 89.2 Globlastp 1879LYM418 tea|gb171|FE861249 3815 598 89.2 Globlastp 1880 LYM418physcomitrella|10v1|BJ940377_P1 3816 598 89.2 Globlastp 1881 LYM418apple|gb171|CN494842_P1 3812 598 89.2 Globlastp 1882 LYM418sunflower|10v1|CD854431_P1 3807 598 89.2 Globlastp 1883 LYM418lettuce|10v1|DW099010_P1 3807 598 89.2 Globlastp 1884 LYM418strawberry|11v1|EX686189_P1 3817 598 89.2 Globlastp 1885 LYM418lettuce|10v1|DW060973_P1 3807 598 89.2 Globlastp 1886 LYM418rhizophora|10v1|SRR005793S0007723_T1 3818 598 89.19 Glotblastn 1887LYM418 cotton|gb164|BE055248 3819 598 89.19 Glotblastn 1888 LYM418citrus|gb166|BQ625142_T1 3820 598 89.19 Glotblastn 1889 LYM418spurge|gb161|BG381771 3821 598 89.19 Glotblastn 1890 LYM418wheat|10v2|CA618761_T1 3822 598 89.19 Glotblastn 1890 LYM418wheat|gb164|CA618761 3823 598 89.19 Glotblastn 1891 LYM418basilicum|10v1|DY334993XX1JT1 — 598 89.19 Glotblastn 1892 LYM418melon|gb165|AM714995 3824 598 87.84 Glotblastn 1893 LYM418canola|10v1|CD831654_P1 3825 598 87.8 Globlastp 1894 LYM418cleome_gynandra|10v1|SRR015532S0006189_P1 3826 598 87.8 Globlastp 1895LYM418 cleome_spinosa|10v1|SRR015531S0107223_P1 3827 598 87.8 globlastp1896 LYM418 eggplant|10v1|FS001074_P1 3828 598 87.8 Globlastp 1897LYM418 guizotia|10v1|GE552831_P1 3829 598 87.8 Globlastp 1898 LYM418momordica|10v1|SRR071315S0001036_P1 3830 598 87.8 Globlastp 1899 LYM418oak|10v1|DN950448_P1 3831 598 87.8 Globlastp 1900 LYM418podocarpus|10v1|SRR065014S0022655_P1 3832 598 87.8 Globlastp 1901 LYM418podocarpus|10v1|SRR065014S0040756_P1 3832 598 87.8 Globlastp 1902 LYM418potato|10v1|BE919537_P1 3833 598 87.8 Globlastp 1903 LYM418salvia|10v1|SRR014553S0000375_P1 3834 598 87.8 Globlastp 1904 LYM418melon|10v1|AM714995_P1 3830 598 87.8 Globlastp 1905 LYM418canola|gb161|CD831654 3825 598 87.8 Globlastp 1906 LYM418antirrhinum|gb166|AJ559184_P1 3835 598 87.8 Globlastp 1907 LYM418radish|gb164|EX754681 3825 598 87.8 Globlastp 1908 LYM418castorbean|09v1|XM002532693_P1 3836 598 87.8 Globlastp 1909 LYM418fern|gb171|DK944489_P1 3837 598 87.8 Globlastp 1910 LYM418potato|gb157.2|BE919537 3833 598 87.8 Globlastp 1911 LYM418b_rapa|gb162|EE519023_P1 3825 598 87.8 Globlastp 1912 LYM418walnuts|gb166|EL891946 3838 598 87.8 Globlastp 1913 LYM418tomato|09v1|BG127484 3833 598 87.8 Globlastp 1914 LYM418centaurea|gb166|EH741303_P1 3839 598 87.8 Globlastp 1915 LYM418potato|gb157.2|BI433807 3833 598 87.8 Globlastp 1916 LYM418walnuts|gb166|CV196253 3838 598 87.8 Globlastp 1917 LYM418zamia|gb166|FD764795 3840 598 87.8 Globlastp 1918 LYM418medicago|09v1|AL378329_P1 3841 598 87.8 Globlastp 1919 LYM418oak|10v1|FP063260_P1 3831 598 87.8 Globlastp 1920 LYM418oak|gb170|DN950448 3831 598 87.8 Globlastp 1921 LYM418liquorice|gb171|FS260075_P1 3842 598 87.8 Globlastp 1922 LYM418medicago|09v1|LLBI310627_P1 3843 598 87.8 Globlastp 1923 LYM418cucumber|09v1|CO997774_P1 3830 598 87.8 Globlastp 1924 LYM418solanum_phureja|09v1|SPHBG127484 3833 598 87.8 globlastp 1925 LYM418tobacco|gb162|EB683494 3844 598 87.8 Globlastp 1926 LYM418papaya|gb165|EX228513_P1 3845 598 87.8 Globlastp 1927 LYM418monkeyflower|09v1|DV206332 3846 598 87.8 Globlastp 1928 LYM418monkeyflower|10v1|DV206332_P1 3846 598 87.8 Globlastp 1929 LYM418pepper|gb171|BM065561_P1 3828 598 87.8 Globlastp 1930 LYM418zinnia|gb171|DV017455 3829 598 87.8 Globlastp 1931 LYM418bruguiera|gb166|BP940736_P1 3847 598 87.8 Globlastp 1932 LYM418chestnut|gb170|SRR006295S0020124_P1 3831 598 87.8 Globlastp 1933 LYM418cassava|09v1|DV453159_P1 3836 598 87.8 Globlastp 1934 LYM418potato|10v1|BI433807_P1 3833 598 87.8 Globlastp 1935 LYM418sunflower|gb162|CD854431 3848 598 87.01 Glotblastn 1936 LYM418acacia|10v1|FS584760_P1 3849 598 86.5 Globlastp 1937 LYM418b_juncea|10v2|E6ANDIZ01EHVPH_P1 3850 598 86.5 Globlastp 1938 LYM418canola|10v1|EE451900_P1 3850 598 86.5 Globlastp 1939 LYM418sequoia|10v1|SRR065044S0010052_P1 3851 598 86.5 Globlastp 1940 LYM418taxus|10v1|SRR032523S0000800_P1 3851 598 86.5 Globlastp 1941 LYM418taxus|10v1|SRR065067S0010041_P1 3851 598 86.5 Globlastp 1942 LYM418cryptomeria|gb166|BP174451_P1 3851 598 86.5 Globlastp 1943 LYM418arabidopsis|10v1|AT1G11475_P1 3852 598 86.5 Globlastp 1944 LYM418maize|gb170|LLDQ245343 3850 598 86.5 Globlastp 1945 LYM418b_oleracea|gb161|AM058057_P1 3850 598 86.5 Globlastp 1946 LYM418arabidopsis_lyrata|09v1|JGIAL001174_P1 3852 598 86.5 Globlastp 1947LYM418 lotus|09v1|LLBW594358_P1 3853 598 86.5 Globlastp 1948 LYM418brachypodium|09v1|GT799495_P1 3854 598 86.5 Globlastp 1949 LYM418canola|10v1|BNU12133_P1 3850 598 86.5 Globlastp 1950 LYM418canola|gb161|BNU12133 3850 598 86.5 globlastp 1951 LYM418lotus|09v1|BW595213_P1 3853 598 86.5 Globlastp 1952 LYM418chickpea|09v2|GR397948_P1 3855 598 86.5 Globlastp 1953 LYM418b_rapa|gb162|CX269260_P1 3850 598 86.5 Globlastp 1954 LYM418canola|10v1|DY006722_P1 3850 598 86.5 Globlastp 1955 LYM418canola|gb161|DY006722 3850 598 86.5 Globlastp 1956 LYM418b_juncea|10v2|E6ANDIZ01BWWKH_P1 3850 598 86.5 Globlastp 1957 LYM418b_juncea|gb164|EVGN01227624003276 3850 598 86.5 Globlastp 1958 LYM418radish|gb164|EX888737 3850 598 86.5 Globlastp 1959 LYM418canola|10v1|CD819775_P1 3850 598 86.5 Globlastp 1960 LYM418canola|gb161|CD819775 3850 598 86.5 Globlastp 1961 LYM418clover|gb162|BB930543_P1 3856 598 86.5 Globlastp 1962 LYM418thellungiella|gb167|BY829924 3857 598 86.5 Globlastp 1963 LYM418radish|gb164|EV525321 3850 598 86.5 Globlastp 1964 LYM418lotus|09v1|BW599973_P1 3853 598 86.5 Globlastp 1965 LYM418soybean|11v1|GLYMA06G10100_P1 3858 598 86.5 Globlastp 1966 LYM418soybean|gb168|BU964480 3858 598 86.5 Globlastp 1967 LYM418soybean|11v1|GLYMA04G10140_P1 3859 598 86.5 Globlastp 1968 LYM418ceratodon|10v1|SRR074892S0702482_T1 3860 598 86.49 Glotblastn 1969LYM418 lettuce|gb157.2|DW099010 3861 598 85.7 Globlastp 1970 LYM418cenchrus|gb166|EB656453_P1 3862 598 85.3 Globlastp 1971 LYM418millet|10v1|EVO454PM030932_P1 3863 598 85.3 Globlastp 1972 LYM418cowpea|gb166|FF392803_T1 3864 598 85.14 Glotblastn 1973 LYM418tamarix|gb166|EG970875 3865 598 85.14 Glotblastn 1974 LYM418cotton|gb164|ES808379 3866 598 85.14 Glotblastn 1975 LYM418triphysaria|10v1|EY006973_T1 3867 598 85.14 Glotblastn 1976 LYM418triphysaria|gb164|EY006973 3867 598 85.14 Glotblastn 1977 LYM418pigeonpea|10v1|SRR054580S0037161_P1 3868 598 85.1 Globlastp 1978 LYM418sciadopitys|10v1|SRR065035S0004294_P1 3869 598 85.1 globlastp 1979LYM418 soybean|gb168|BI310627 3870 598 85.1 Globlastp 1980 LYM418oak|10v1|DN949703_P1 3871 598 85.1 Globlastp 1981 LYM418oak|gb170|DN949703 3871 598 85.1 Globlastp 1982 LYM418cowpea|gb166|FF390921_P1 3872 598 85.1 Globlastp 1983 LYM418spikemoss|gb165|DN838654 3873 598 85.1 Globlastp 1984 LYM418bean|gb167|CA902225_P1 3872 598 85.1 Globlastp 1985 LYM418peanut|10v1|EE126621_P1 3874 598 85.1 Globlastp 1986 LYM418peanut|gb171|EE126621 3874 598 85.1 Globlastp 1987 LYM418chestnut|gb170|SRR006295S0024295_P1 3871 598 85.1 Globlastp 1988 LYM418mesostigma|gb166|DN259476_P1 3875 598 85.1 Globlastp 1989 LYM418soybean|11v1|GLYMA01G03590_P1 3876 598 85.1 Globlastp 1990 LYM418soybean|gb168|BI969339 3876 598 85.1 Globlastp 1991 LYM418soybean|gb168|BQ785621 3876 598 85.1 Globlastp 1992 LYM418peanut|10v1|CD038619_P1 3874 598 85.1 Globlastp 1993 LYM418peanut|gb171|CD038619 3874 598 85.1 Globlastp 1994 LYM418peanut|10v1|EE123543_P1 3874 598 85.1 Globlastp 1995 LYM418peanut|gb171|EE123543 3874 598 85.1 Globlastp 1996 LYM418radish|gb164|EV569312 3877 598 85.1 Globlastp 1997 LYM418millet|09v1|EVO454PM030932 3878 598 84 Glotblastn 1998 LYM418gnetum|10v1|SRR064399S0056000_P1 3879 598 83.8 Globlastp 1999 LYM418arabidopsis|10v1|AT1G61700_P1 3880 598 83.8 Globlastp 2000 LYM418arabidopsis_lyrata|09v1|JGIAL005777_P1 3880 598 83.8 Globlastp 2001LYM418 maize|gb170|LLFL220754 3881 598 83.78 Glotblastn 2002 LYM418lettuce|gb157.2|DW118622 3882 598 83.5 Globlastp 2003 LYM418poppy|gb166|FE965029_T1 — 598 83.13 Glotblastn 2004 LYM418aquilegia|10v2|JGIAC019942_P1 3883 598 82.7 Globlastp 2005 LYM418lettuce|gb157.2|DW060973 3884 598 82.5 Globlastp 2006 LYM418artemisia|10v1|EY067798_T1 3885 598 82.43 glotblastn 2007 LYM418eggplant|10v1|FS071046_T1 3886 598 82.43 Glotblastn 2008 LYM418cotton|gb164|DR456367 3887 598 82.35 Glotblastn 2009 LYM418strawberry|gb164|EX686189 3888 598 81.5 Globlastp 2010 LYM418cotton|gb164|BF274001 3889 598 81.18 Glotblastn 2011 LYM418cynodon|10v1|ES300419_P1 3890 598 81.1 Globlastp 2012 LYM418sorghum|09v1|SB05G005840 3891 598 81.1 Globlastp 2013 LYM418chlamydomonas|gb162|AV387081_T1 3892 598 81.08 Glotblastn 2014 LYM418cucumber|09v1|CSCRP016122_T1 3893 598 81.08 Glotblastn 2015 LYM418zinnia|gb171|DV017458 — 598 80.25 Glotblastn 2016 LYM421maize|10v1|AI861160_P1 3894 600 94.6 Globlastp 2017 LYM421maize|gb170|AI861160 3895 600 93.8 Globlastp 2018 LYM421rice|gb170|OS03G12730 3896 600 86.7 Globlastp 2019 LYM421brachypodium|09v1|SRR031796S0016409_P1 3897 600 84.5 Globlastp 2020LYM435 maize|10v1|DT648738_P1 3898 605 85.5 Globlastp 2021 LYM435sorghum|09v1|SB01G001585 3899 605 82.23 Glotblastn 2022 LYM436maize|10v1|AW455688_P1 3900 606 91.1 Globlastp 2023 LYM436maize|gb170|AW455688 3900 606 91.1 Globlastp 2024 LYM436switchgrass|gb167|FE600197 3901 606 84.4 Globlastp 2025 LYM437maize|10v1|AI601005_P1 3902 607 98.3 Globlastp 2026 LYM437maize|gb170|AI601005 3902 607 98.3 Globlastp 2027 LYM437maize|10v1|AI637244_P1 3903 607 97.5 Globlastp 2028 LYM437maize|gb170|AI637244 3903 607 97.5 Globlastp 2029 LYM437switchgrass|gb167|FE606343 3904 607 97.2 Globlastp 2030 LYM437cenchrus|gb166|EB660720_P1 3905 607 97.2 Globlastp 2031 LYM437rice|gb170|OS03G57870 3906 607 94.6 Globlastp 2032 LYM437brachypodium|09v1|DV472921_P1 3907 607 91.8 Globlastp 2033 LYM437wheat|10v2|BG274587_P1 3908 607 90.4 Globlastp 2034 LYM437wheat|gb164|BE402399 3908 607 90.4 Globlastp 2035 LYM437barley|10v2|AV836369_P1 3909 607 90.4 Globlastp 2036 LYM437barley|gb157SOLEXA|AL505233 3909 607 90.4 globlastp 2037 LYM437foxtail_millet|10v2|SICRP014018_T1 3910 607 84.18 Glotblastn 2038 LYM437aquilegia|10v2|JGIAC004711_P1 3911 607 82.2 Globlastp 2039 LYM437ipomoea|gb157.2|CJ752953 3912 607 81.4 Globlastp 2040 LYM437aristolochia|10v1|FD750372_P1 3913 607 80.8 Globlastp 2041 LYM437cichorium|gb171|EH675731_T1 3914 607 80.79 Glotblastn 2042 LYM437soybean|11v1|BU549346_P1 3915 607 80.5 Globlastp 2043 LYM437soybean|gb168|AW585000 3915 607 80.5 Globlastp 2044 LYM437oat|10v2|GO596333_T1 3916 607 80.23 Glotblastn 2045 LYM437eggplant|10v1|FS006898_P1 3917 607 80.2 Globlastp 2046 LYM437solanum_phureja|09v1|SPHBG643831 3918 607 80.2 Globlastp 2047 LYM438maize|10v1|CD941418_P1 3919 608 88.3 Globlastp 2048 LYM438maize|gb170|CD941418 3919 608 88.3 Globlastp 2049 LYM438foxtail_millet|10v2|SICRP019893_T1 3920 608 85.64 Glotblastn 2050 LYM438switchgrass|gb167|FE622579 3921 608 85.2 Globlastp 2051 LYM438rice|gb170|OS03G51580 3922 608 80.7 Globlastp 2052 LYM440maize|gb170|BG319904 3923 610 87.3 Globlastp 2052 LYM440maize|10v1|BG319904_P1 4276 718 87.2 Globlastp 2053 LYM440millet|10v1|EVO454PM003935_P1 3924 610 86.3 Globlastp 2054 LYM440rice|gb170|OS08G14440_P1 3925 610 80.6 Globlastp 2055 LYM441rice|gb170|OS10G42490 3926 611 80.1 Globlastp 2056 LYM442sugarcane|10v1|BQ529804_P1 3927 612 93.7 Globlastp 2057 LYM442sugarcane|gb157.3|BQ529804 3927 612 93.7 Globlastp 2058 LYM442maize|10v1|BM072707_P1 3928 612 86 Globlastp 2059 LYM442maize|gb170|BM072707 3928 612 86 Globlastp 2060 LYM443sorghum|09v1|SB01G038035 3929 613 90 Globlastp 2061 LYM443switchgrass|gb167|FE619547 3930 613 80.4 globlastp 2062 LYM444maize|10v1|AW787625_P1 3931 614 94.9 Globlastp 2063 LYM444maize|gb170|AW787625 3931 614 94.9 Globlastp 2064 LYM444millet|10v1|PMSLX0044651D1_P1 3932 614 92.7 Globlastp 2065 LYM444maize|10v1|BQ035172_T1 3933 614 90.11 Glotblastn 2066 LYM444maize|gb170|BQ035172 3934 614 90.1 Globlastp 2067 LYM444rice|gb170|OS03G14370 3935 614 87.3 Globlastp 2068 LYM444brachypodium|09v1|SRR031796S0003201_P1 3936 614 84.9 Globlastp 2069LYM444 maize|10v1|ZMCRP2V190058_T1 3937 614 83.78 Glotblastn 2070 LYM446maize|10v1|AW308657_P1 3938 616 95.7 Globlastp 2071 LYM446foxtail_millet|10v2|SICRP034406_P1 3939 616 94.2 Globlastp 2072 LYM446brachypodium|09v1|DV469284_P1 3940 616 90 Globlastp 2073 LYM446maize|gb170|AW308657 3941 616 89.4 Globlastp 2074 LYM446oat|10v2|GR360645_T1 3942 616 87.23 Glotblastn 2075 LYM446barley|10v2|BQ460120_P1 3943 616 86.7 Globlastp 2076 LYM446barley|gb157SOLEXA|BQ460120 3943 616 86.7 Globlastp 2077 LYM446cynodon|10v1|ES301316_P1 3944 616 85 Globlastp 2078 LYM446rice|gb170|OS03G07370 3945 616 84.7 Globlastp 2079 LYM446wheat|10v2|CA700687_P1 3946 616 83.7 Globlastp 2080 LYM446wheat|gb164|CA635851 3947 616 81.2 Globlastp 2081 LYM447maize|10v1|DW780791_P1 3948 617 92.2 Globlastp 2082 LYM447maize|gb170|DW780791 3949 617 87.8 Globlastp 2083 LYM447foxtail_millet|10v2|SICRP016156_T1 3950 617 85.69 Glotblastn 2084 LYM447brachypodium|09v1|SRR031798S0273458_T1 3951 617 81.36 Glotblastn 2085LYM448 maize|gb170|AW787353 3952 618 92.8 Globlastp 2086 LYM448maize|10v1|AW787353_P1 3953 618 92.1 Globlastp 2087 LYM448switchgrass|gb167|FE654400 3954 618 85.3 Globlastp 2088 LYM448sugarcane|10v1|CA145812_P1 3955 618 82.7 Globlastp 2089 LYM448sugarcane|gb157.3|CA145812 3956 618 82.4 globlastp 2090 LYM449maize|10v1|AW147097_P1 3957 619 90.9 Globlastp 2091 LYM449maize|gb170|AW147097 3957 619 90.9 Globlastp 2092 LYM449rice|gb170|OS03G02330 3958 619 80.3 Globlastp 2093 LYM450maize|10v1|CO528205_P1 3959 620 80.5 Globlastp 2094 LYM450maize|gb170|CO528205 3960 620 80.3 Globlastp 2095 LYM452maize|gb170|BQ619125 3961 622 85.4 Globlastp 2096 LYM452maize|10v1|BQ619125_P1 3962 622 84.6 Globlastp 2097 LYM453maize|10v1|AI461465_P1 3963 623 89.9 Globlastp 2098 LYM453maize|gb170|AI670283 3963 623 89.9 Globlastp 2099 LYM453maize|gb170|CF630644 3964 623 88.5 Globlastp 2100 LYM453maize|10v1|CF630644_P1 3965 623 88 Globlastp 2101 LYM454maize|10v1|AI586492_P1 3966 624 95.6 Globlastp 2102 LYM454switchgrass|gb167|FE610910 3967 624 91.25 Glotblastn 2103 LYM454rice|gb170|OS07G38260 3968 624 84 Globlastp 2104 LYM454wheat|gb164|BE400205 3969 624 83.7 Globlastp 2105 LYM454barley|10v2|BE413033_P1 3970 624 83.3 Globlastp 2106 LYM454barley|gb157SOLEXA|AL450715 3970 624 83.3 Globlastp 2107 LYM454brachypodium|09v1|DV472226_P1 3971 624 83.2 Globlastp 2108 LYM454foxtail_millet|10v2|SICRP004784_T1 3972 624 83.17 Glotblastn 2109 LYM454wheat|10v2|BE405727_P1 3973 624 83.1 Globlastp 2110 LYM455maize|10v1|DT941652_P1 3974 625 84.7 Globlastp 2111 LYM455maize|gb170|DT941652 3974 625 84.7 Globlastp 2112 LYM457sugarcane|gb157.3|CA075773 3975 627 86.2 Globlastp 2113 LYM458wheat|gb164|CA484331 3976 628 94.44 Glotblastn 2114 LYM458wheat|10v2|CA484331_P1 3977 628 94.4 Globlastp 2115 LYM458sugarcane|10v1|BQ533118_P1 3978 628 89.7 Globlastp 2116 LYM458sugarcane|gb157.3|BQ533118 3979 628 89.7 Globlastp 2117 LYM458sugarcane|gb157.3|CA102307 3980 628 89 Globlastp 2118 LYM458sugarcane|gb157.3|BQ533973 3981 628 88.9 Globlastp 2119 LYM458sugarcane|10v1|BQ533973_P1 3981 628 88.9 Globlastp 2120 LYM458sugarcane|10v1|CA102307_T1 3982 628 88.19 glotblastn 2121 LYM461foxtail_millet|10v2|FXTRMSLX01618069D1_P1 3983 630 96.9 Globlastp 2122LYM461 maize|10v1|AI491437_P1 3984 630 96.9 Globlastp 2123 LYM461maize|gb170|AI491437 3984 630 96.9 Globlastp 2124 LYM461rice|gb170|OS01G44110 3985 630 91.8 Globlastp 2125 LYM461brachypodium|09v1|DV482433_P1 3986 630 90.8 Globlastp 2126 LYM461barley|10v2|BE196273_P1 3987 630 89.6 Globlastp 2127 LYM461wheat|10v2|BE406810_P1 3988 630 89.5 Globlastp 2128 LYM461wheat|gb164|BE400643 3989 630 89.5 Globlastp 2129 LYM461wheat|10v2|BE500293_T1 3990 630 88.48 Glotblastn 2130 LYM461switchgrass|gb167|DN144010 3991 630 80.6 Globlastp 2131 LYM464maize|10v1|BI273479_P1 3992 632 91 Globlastp 2132 LYM464maize|gb170|BI273479 3992 632 91 Globlastp 2133 LYM464foxtail_millet|10v2|SICRP024464_T1 3993 632 81.51 Glotblastn 2134 LYM466rice|gb170|OS01G67220 3994 634 88.8 Globlastp 2135 LYM466brachypodium|09v1|DV469198_P1 3995 634 83 Globlastp 2136 LYM467maize|gb170|AI987474 3996 635 93.8 Globlastp 2137 LYM467maize|10v1|AI987474_P1 3997 635 93.7 Globlastp 2138 LYM467maize|10v1|AI902162_P1 3998 635 92.8 Globlastp 2139 LYM468maize|10v1|AW067000_T1 3999 636 84.86 Glotblastn 2140 LYM468maize|gb170|AW067000 4000 636 81.12 Glotblastn 2141 LYM468rice|gb170|OS01G72350 4001 636 80 Globlastp 2142 LYM473maize|10v1|AW181144_P1 4002 639 93.6 Globlastp 2143 LYM473maize|gb170|AW181144 4002 639 93.6 Globlastp 2144 LYM473millet|10v1|EVO454PM001191_P1 4003 639 90.9 Globlastp 2145 LYM473switchgrass|gb167|FE630245 4004 639 90.4 Globlastp 2146 LYM473brachypodium|09v1|DV479845_P1 4005 639 85.2 Globlastp 2147 LYM473rice|gb170|OS01G27940 4006 639 84.8 Globlastp 2148 LYM473brachypodium|09v1|DV481394_T1 4007 639 84.76 Glotblastn 2149 LYM473cenchrus|gb166|EB653347_P1 4008 639 83.5 Globlastp 2150 LYM473wheat|10v2|BE497866_P1 4009 639 82.7 Globlastp 2151 LYM473barley|gb157SOLEXA|BI952752 4010 639 82.42 Glotblastn 2152 LYM473barley|10v2|BG366664_P1 4011 639 82.3 globlastp 2153 LYM473wheat|gb164|BE497866 4012 639 82.19 Glotblastn 2154 LYM473oat|10v2|CN821117_T1 4013 639 81.58 Glotblastn 2155 LYM474maize|10v1|BM259128_P1 4014 640 91.4 Globlastp 2156 LYM474maize|gb170|BM259128 4014 640 91.4 Globlastp 2157 LYM474switchgrass|gb167|FL787161 4015 640 80.95 Glotblastn 2158 LYM474switchgrass|gb167|FL954360 4016 640 80.8 Globlastp 2159 LYM474foxtail_millet|10v2|SICRP022522_T1 4017 640 80.66 Glotblastn 2160 LYM474sugarcane|10v1|CA228273_P1 4018 640 80.5 Globlastp 2161 LYM474foxtail_millet|10v2|FXTRMSLX00498195D1_T1 4019 640 80.19 Glotblastn 2162LYM476 sugarcane|10v1|CF569829_P1 4020 642 84.7 Globlastp 2163 LYM476sugarcane|gb157.3|CF569829 4021 642 84.7 Globlastp 2164 LYM477maize|10v1|CD661856_P1 4022 643 94.8 Globlastp 2165 LYM477maize|gb170|CD661856 4022 643 94.8 Globlastp 2166 LYM477sugarcane|gb157.3|CA275566 4023 643 94.27 Glotblastn 2167 LYM478maize|10v1|BM072994_P1 4024 644 90.2 Globlastp 2168 LYM478sugarcane|gb157.3|BQ535919 4025 644 90.1 Globlastp 2169 LYM478sugarcane|gb157.3|CA138971 4026 644 90.1 Globlastp 2170 LYM478sugarcane|gb157.3|CA131868 4027 644 90.1 Globlastp 2171 LYM478sugarcane|10v1|BQ535919_P1 4027 644 90.1 Globlastp 2172 LYM478maize|gb170|BG837042 4028 644 89.91 Glotblastn 2173 LYM478maize|10v1|BG837042_P1 4029 644 89.9 Globlastp 2174 LYM478sugarcane|gb157.3|CA074917 4030 644 89.2 Globlastp 2175 LYM478sugarcane|gb157.3|CA109848 4031 644 89.2 Globlastp 2176 LYM478sugarcane|gb157.3|CA130732 4032 644 89.2 Globlastp 2177 LYM478maize|gb170|LLCO451725 4033 644 88.99 Glotblastn 2178 LYM478sugarcane|gb157.3|CA158243 4034 644 87.39 glotblastn 2179 LYM478sugarcane|gb157.3|CA094465 4035 644 86.49 Glotblastn 2180 LYM478sugarcane|gb157.3|CA132959 4036 644 86.49 Glotblastn 2181 LYM478sugarcane|gb157.3|CA144364 4037 644 83.8 Globlastp 2182 LYM478switchgrass|gb167|DN142212 4038 644 83.5 Globlastp 2183 LYM478cynodon|10v1|ES299681_P1 4039 644 81.8 Globlastp 2184 LYM480foxtail_millet|10v2|OXFXTSLX00010151D1T1_T1 4040 646 81.1 Glotblastn2185 LYM480 sorghum|09v1|SB08G001950 4041 646 80.38 Glotblastn 2186LYM480 sugarcane|10v1|CA067698_P1 4042 646 80.2 Globlastp 2187 LYM481maize|10v1|AW202494_P1 4043 647 82.6 Globlastp 2188 LYM481maize|gb170|AW202494 4043 647 82.6 Globlastp 2189 LYM483sorghum|09v1|SB05G018443 4044 648 89.45 Glotblastn 2190 LYM485maize|gb170|AW147025 4045 650 92.6 Globlastp 2191 LYM485maize|10v1||AW147025_P1 4046 650 89.7 Globlastp 2192 LYM485rice|gb170|OS04G42840 4047 650 87.1 Globlastp 2193 LYM485brachypodium|09v1|DV476624_P1 4048 650 85.9 Globlastp 2194 LYM486maize|10v1|AI734670_P1 4049 651 96.1 Globlastp 2195 LYM486maize|gb170|AI734670 4049 651 96.1 Globlastp 2196 LYM486brachypodium|09v1|DV479343_P1 4050 651 89.1 Globlastp 2197 LYM486rice|gb170|OS04G46180 4051 651 88.8 Globlastp 2198 LYM486wheat|10v2|BE446780_P1 4052 651 88.3 Globlastp 2199 LYM486barley|10v2|BF622824_P1 4053 651 88 Globlastp 2200 LYM486wheat|10v2|BE446356_P1 4054 651 88 Globlastp 2201 LYM486foxtail_millet|10v2|SICRP012708_P1 4055 651 81.5 Globlastp 2202 LYM487sugarcane|10v1|CA082908_P1 4056 652 99 Globlastp 2203 LYM487sugarcane|gb157.3|CA082908 4056 652 99 Globlastp 2204 LYM487maize|10v1|AI619147_P1 4057 652 98.5 Globlastp 2205 LYM487maize|gb170|AI619147 4057 652 98.5 Globlastp 2206 LYM487maize|10v1|BQ294334_P1 4058 652 97.5 Globlastp 2207 LYM487maize|gb170|BQ294334 4058 652 97.5 globlastp 2208 LYM487switchgrass|gb167|FL703852 4059 652 97.1 Globlastp 2209 LYM487millet|09v1|EVO454PM008070 4060 652 96.3 Globlastp 2210 LYM487millet|10v1|EVO454PM008070_T1 4061 652 96.27 Glotblastn 2211 LYM487rice|gb170|OS08G13350 4062 652 92.1 Globlastp 2212 LYM487brachypodium|09v1|GT776449_P1 4063 652 88 Globlastp 2213 LYM487rice|gb170|OS04G51710 4064 652 87.1 Globlastp 2214 LYM487brachypodium|09v1|GT772403_P1 4065 652 86.9 Globlastp 2215 LYM487millet|09v1|CD726424 4066 652 86.3 Globlastp 2216 LYM487millet|10v1|CD726424_P1 4066 652 86.3 Globlastp 2217 LYM487sugarcane|gb157.3|CA084353 4067 652 86.3 Globlastp 2218 LYM487sugarcane|10v1|CA084353_P1 4068 652 86.1 Globlastp 2219 LYM487wheat|10v2|BE400599_P1 4069 652 85.9 Globlastp 2220 LYM487wheat|gb164|BE400599 4069 652 85.9 Globlastp 2221 LYM487switchgrass|gb167|FE623823 4070 652 85.5 Globlastp 2222 LYM487sorghum|09v1|SB07G006900 4071 652 85.5 Globlastp 2223 LYM487maize|10v1|AW129881_P1 4072 652 85.3 Globlastp 2224 LYM487maize|gb170|AW129881 4072 652 85.3 Globlastp 2225 LYM487maize|10v1|AW282193_P1 4073 652 85.1 Globlastp 2226 LYM487brachypodium|09v1|DV481308_P1 4074 652 85.1 Globlastp 2227 LYM487barley|10v2|AV833313_P1 4075 652 81.8 Globlastp 2228 LYM487barley|gb157SOLEXA|AV833313 4075 652 81.8 Globlastp 2229 LYM487wheat|10v2|BE445358_P1 4076 652 81.8 Globlastp 2230 LYM487wheat|gb164|BQ801650 4077 652 81.4 Globlastp 2231 LYM487zostera|10v1|SRR057351S0019718_P1 4078 652 80.3 Globlastp 2232 LYM489sugarcane|gb157.3|CA101920 4079 654 96.4 Globlastp 2233 LYM489sorghum|09v1|SB06G030750 4080 654 94 Globlastp 2234 LYM489maize|10v1|BI595677_P1 4081 654 90.5 Globlastp 2235 LYM489maize|gb170|BI595677 4081 654 90.5 Globlastp 2236 LYM489maize|10v1|AA979922_P1 4082 654 85.7 globlastp 2237 LYM489maize|gb170|AA979922 4082 654 85.7 Globlastp 2238 LYM490maize|10v1|W21761_P1 4083 655 92.3 Globlastp 2239 LYM490maize|gb170|W21761 4083 655 92.3 Globlastp 2240 LYM490foxtail_millet|10v2|SICRP032859_P1 4084 655 91.4 Globlastp 2241 LYM490rice|gb170|OS04G57310 4085 655 82.9 Globlastp 2242 LYM490oat|10v2|GR330176_P1 4086 655 80.8 Globlastp 2243 LYM490brachypodium|09v1|DV481980_P1 4087 655 80.6 Globlastp 2244 LYM491maize|10v1|DV171526_P1 4088 656 89.1 Globlastp 2245 LYM491maize|gb170|DV171526 4088 656 89.1 Globlastp 2246 LYM491foxtail_millet|10v2|SICRP012980_T1 4089 656 80.69 Glotblastn 2247 LYM493maize|10v1|AW287758_P1 4090 658 97.6 Globlastp 2248 LYM493maize|gb170|AW287758 4090 658 97.6 Globlastp 2249 LYM493switchgrass|gb167|FL704106 4091 658 93.8 Globlastp 2250 LYM493foxtail_millet|10v2|EC612467_P1 4092 658 89.7 Globlastp 2251 LYM493brachypodium|09v1|GT766073_P1 4093 658 87.6 Globlastp 2252 LYM493barley|10v2|BE412717_P1 4094 658 87.6 Globlastp 2253 LYM493barley|gb157SOLEXA|BE412717 4094 658 87.6 Globlastp 2254 LYM493wheat|10v2|BQ842285_P1 4095 658 87.4 Globlastp 2255 LYM493wheat|gb164|BE430947 4096 658 87.4 Globlastp 2256 LYM493rice|gb170|OS08G04630 4097 658 86.14 Glotblastn 2257 LYM493millet|09v1|EVO454PM003547 4098 658 82.6 Globlastp 2258 LYM495maize|gb170|AI491510 4099 660 93.1 Globlastp 2259 LYM495maize|10v1|AI491510_P1 4100 660 92.8 Globlastp 2260 LYM497sorghum|09v1|SB05G000365 4101 662 85 Globlastp 2261 LYM497maize|10v1|CA404468_P1 4102 662 83.3 Globlastp 2262 LYM497maize|gb170|CA404468 4103 662 82.5 Globlastp 2263 LYM498maize|10v1|AW331749_P1 4104 663 96.5 Globlastp 2264 LYM498maize|gb170|AW331749 4104 663 96.5 Globlastp 2265 LYM498maize|10v1|GRMZM2G014329T01_P1 4105 663 96.2 Globlastp 2266 LYM498millet|10v1|EVO454PM063336_P1 4106 663 92.9 globlastp 2267 LYM498millet|09v1|EVO454PM063336 4107 663 92.7 Globlastp 2268 LYM498foxtail_millet|10v2|SICRP020841_T1 4108 663 92.69 Glotblastn 2269 LYM498rice|gb170|OSHG01875 4109 663 88.75 Glotblastn 2270 LYM498rice|gb170|OS12G01930 4110 663 87.7 Globlastp 2271 LYM498wheat|10v2|BE415292_P1 4111 663 84.6 Globlastp 2272 LYM498wheat|gb164|BE415292 4112 663 84.4 Globlastp 2273 LYM498brachypodium|09v1|DV477205_P1 4113 663 80 Globlastp 2274 LYM499maize|10v1|AI491601_P1 4114 664 87.8 Globlastp 2275 LYM500maize|10v1|CF045034_P1 4115 665 83.9 Globlastp 2276 LYM500maize|gb170|CF045034 4115 665 83.9 Globlastp 2277 LYM502maize|10v1|CF046508_P1 4116 667 89.6 Globlastp 2278 LYM502maize|gb170|CF046508 4116 667 89.6 Globlastp 2279 LYM502switchgrass|gb167|FL824724 4117 667 89 Globlastp 2280 LYM502maize|10v1|BG320787_P1 4118 667 85.8 Globlastp 2281 LYM502maize|gb170|BG320787 4118 667 85.8 Globlastp 2282 LYM504maize|10v1|AA051885_P1 4119 669 89.1 Globlastp 2283 LYM504maize|gb170|AA051885 4119 669 89.1 Globlastp 2284 LYM504switchgrass|gb167|FE651560 4120 669 85.7 Globlastp 2285 LYM504oat|10v2|CN820747_P1 4121 669 82.2 Globlastp 2286 LYM504brachypodium|09v1|GT763669_P1 4122 669 81.7 Globlastp 2287 LYM504rice|gb170|OS12G43130 4123 669 81 Globlastp 2288 LYM504barley|10v2|BE422206_P1 4124 669 80.9 Globlastp 2289 LYM504wheat|10v2|BE425225_P1 4125 669 80.6 Globlastp 2290 LYM504wheat|gb164|BE425225 4126 669 80.39 Glotblastn 2291 LYM504leymus|gb166|EG378293_T1 4127 669 80.24 Glotblastn 2292 LYM505sugarcane|10v1|CA065398_P1 4128 670 91.9 Globlastp 2293 LYM505sugarcane|gb157.3|CA065398 4128 670 91.9 Globlastp 2294 LYM505foxtail_millet|10v2|OXFXTSLX00011066T1_P1 4129 670 85.5 Globlastp 2295LYM505 millet|10v1|PMSLX0012653D2_P1 4130 670 84.8 Globlastp 2296 LYM505switchgrass|gb167|FE603625 4131 670 84 Globlastp 2297 LYM507maize|10v1|AI948254_P1 4132 672 81.1 globlastp 2298 LYM509maize|10v1|AW927894_P1 4133 674 92.6 Globlastp 2299 LYM509sugarcane|10v1|CA087363_P1 4134 674 87.1 Globlastp 2300 LYM509sugarcane|gb157.3|CA087363 4135 674 86.6 Globlastp 2301 LYM509switchgrass|gb167|FL699837 4136 674 85.53 Glotblastn 2302 LYM509foxtail_millet|10v2|OXEC613219T1_P1 4137 674 83.5 Globlastp 2303 LYM509millet|10v1|EVO454PM016056_P1 4138 674 83.5 Globlastp 2304 LYM510barley|gb157SOLEXA|BI949234 4139 675 81.95 Glotblastn 2305 LYM368_H4switchgrass|gb167|FL694165_P1 4140 679 81.9 Globlastp 2306 LYM312wheat|gb164|AL822986 4141 686 93.21 Glotblastn 2307 LYM312wheat|10v2|BE500856_T1 4142 686 91.7 Glotblastn 2308 LYM312oat|10v2|CN815344_T1 4143 686 86.84 Glotblastn 2309 LYM312brachypodium|09v1|GT849852_T1 4144 686 83.77 Glotblastn 2310 LYM312rice|gb170|OS05G25450 4145 686 83.77 Glotblastn 2311 LYM312sorghum|09v1|SB06G029710 4146 686 83.08 Glotblastn 2312 LYM312sugarcane|10v1|BQ804036_T1 4147 686 83.08 Glotblastn 2313 LYM312sugarcane|gb157.3|BQ804036 4148 686 82.71 Glotblastn 2314 LYM312millet|09v1|EVO454PM008579 4149 686 81.13 Glotblastn 2315 LYM312millet|10v1|EVO454PM008579_T1 4150 686 81.13 Glotblastn 2316 LYM315switchgrass|gb167|FE607688 4151 687 84.09 Glotblastn 2317 LYM315sorghum|09v1|SB03G008585 4152 687 83.36 Glotblastn 2318 LYM315maize|10v1|AW076289_T1 4153 687 83 Glotblastn 2319 LYM315maize|gb170|AW076289 4153 687 83 Glotblastn 2320 LYM315maize|10v1|BG840481_T1 4154 687 80.47 Glotblastn 2321 LYM316wheat|10v2|BE606637XX1_T1 4155 688 94.1 Glotblastn 2322 LYM316maize|gb170|AI947455 4156 688 93.9 Glotblastn 2323 LYM316millet|10v1|EVO454PM020446_T1 4157 688 91.64 Glotblastn 2324 LYM316sorghum|09v1|SB01G011610 4158 688 91.46 glotblastn 2325 LYM316millet|09v1|EVO454PM003214 4159 688 91.4 Globlastp 2326 LYM316castorbean|09v1|XM002511462_T1 4160 688 89.55 Glotblastn 2327 LYM316lotus|09v1|GO023600_T1 4161 688 89.37 Glotblastn 2328 LYM316cotton|gb164|BF268247 4162 688 89.02 Glotblastn 2329 LYM316prunus|10v1|CB818450_T1 4163 688 89.02 Glotblastn 2330 LYM316pigeonpea|10v1|SRR054580S0025341_T1 4164 688 88.85 Glotblastn 2331LYM316 artemisia|10v1|EY050657_T1 4165 688 88.5 Glotblastn 2332 LYM316oak|10v1|FP038022_T1 4166 688 88.5 Glotblastn 2333 LYM316sunflower|gb162|CD855840 4167 688 88.5 Glotblastn 2334 LYM316triphysaria|10v1|BE574923_T1 4168 688 88.33 Glotblastn 2335 LYM316arabidopsis_lyrata|09v1|JGIAL030919_T1 4169 688 88.15 Glotblastn 2336LYM316 pepper|gb171|BM067292_T1 4170 688 88.15 Glotblastn 2337 LYM316gnetum|10v1|SRR064399S0004048_T1 4171 688 87.98 Glotblastn 2338 LYM316switchgrass|gb167|FL699125 4172 688 87.8 Glotblastn 2339 LYM316oat|10v2|GO588185_T1 4173 688 87.46 Glotblastn 2340 LYM316taxus|10v1|SRR032523S0016620_T1 4174 688 86.24 Glotblastn 2341 LYM316millet|10v1|EVO454PM006153_P1 4175 688 85.9 Globlastp 2342 LYM316wheat|10v2|BU100161_P1 4176 688 85.9 Globlastp 2343 LYM316lettuce|10v1|DW080995_T1 4177 688 85.54 Glotblastn 2344 LYM316switchgrass|gb167|FE638151 4178 688 85.5 Globlastp 2345 LYM316grape|gb160|CB346136_P1 4179 688 85.5 Globlastp 2346 LYM316wheat|gb164|BU100161 4180 688 85.4 Globlastp 2347 LYM316lettuce|gb157.2|DW080995 4181 688 85.37 Glotblastn 2348 LYM316sunflower|gb162|CD854072 4182 688 85.29 Glotblastn 2349 LYM316sunflower|10v1|CD854072_T1 4183 688 85.12 Glotblastn 2350 LYM316sugarcane|10v1|CA068038_P1 4184 688 84.7 globlastp 2351 LYM316sugarcane|gb157.3|CA068038 4184 688 84.7 Globlastp 2352 LYM316cacao|gb167|CU477584 4185 688 84.2 Globlastp 2353 LYM316maize|10v1|AW172100_T1 4186 688 83.97 Glotblastn 2354 LYM316maize|gb170|AW172100 4186 688 83.97 Glotblastn 2355 LYM316millet|09v1|EVO454PM020446 4187 688 83.9 Globlastp 2356 LYM316triphysaria|10v1|EY002042_T1 4188 688 83.56 Glotblastn 2357 LYM316citrus|gb166|CF504937_P1 4189 688 83.4 Globlastp 2358 LYM316pigeonpea|10v1|SRR054580S0022176_T1 4190 688 82.75 Glotblastn 2359LYM316 spikemoss|gb165|FE433020 4191 688 81.71 Glotblastn 2360 LYM316soybean|gb168|CA901776 4192 688 81.53 Glotblastn 2361 LYM316spikemoss|gb165|FE428833 4193 688 81.53 Glotblastn 2362 LYM316cassava|09v1|CK901350_T1 4194 688 81.46 Glotblastn 2363 LYM316peanut|10v1|EL966922_P1 4195 688 80.7 Globlastp 2364 LYM316marchantia|gb166|BJ841272_T1 4196 688 80.66 Glotblastn 2365 LYM316centaurea|gb166|EL934603_T1 4197 688 80.48 Glotblastn 2366 LYM316melon|10v1|VMEL01572033803113_P1 4198 688 80.3 Globlastp 2367 LYM316millet|09v1|EVO454PM000746 4199 688 80.2 Globlastp 2368 LYM323wheat|gb164|BE213629 4200 689 92.21 Glotblastn 2369 LYM323wheat|gb164|CK213492 4201 689 90.91 Glotblastn 2370 LYM323barley|gb157SOLEXA|BF265424 4202 689 90.91 Glotblastn 2371 LYM323barley|10v2|BI953318_T1 4203 689 89.61 Glotblastn 2372 LYM323pine|10v2|SRR036960S0253724_T1 4204 689 89.61 Glotblastn 2373 LYM323wheat|10v2|BG909365_T1 4205 689 89.61 Glotblastn 2374 LYM323wheat|10v2|CA670391_T1 4206 689 89.61 Glotblastn 2375 LYM323wheat|gb164|BE418436 4207 689 89.61 Glotblastn 2376 LYM323wheat|gb164|CO347212 4208 689 89.61 Glotblastn 2377 LYM323lolium|10v1|AY693395_T1 4209 689 88.31 glotblastn 2378 LYM323oat|10v2|CN817199_T1 4210 689 88.31 Glotblastn 2379 LYM323oat|10v2|CN817812_T1 4211 689 88.31 Glotblastn 2380 LYM323oat|10v2|CN818219_T1 4212 689 88.31 Glotblastn 2381 LYM323oat|10v2|GR321961_T1 4213 689 88.31 Glotblastn 2382 LYM323oat|10v2|GR334226_T1 4214 689 88.31 Glotblastn 2383 LYM323oat|10v2|GR339228_T1 4215 689 88.31 Glotblastn 2384 LYM323oat|10v2|GR339741_T1 4216 689 88.31 Glotblastn 2385 LYM323oat|10v2|GR342788_T1 4217 689 88.31 Glotblastn 2386 LYM323oat|10v2|SRR020741S0000225_T1 4218 689 88.31 Glotblastn 2387 LYM323oat|10v2|SRR020741S0000254_T1 4219 689 88.31 Glotblastn 2388 LYM323oat|10v2|SRR020741S0003459_T1 4217 689 88.31 Glotblastn 2389 LYM323oat|10v2|SRR020741S0004650_T1 4220 689 88.31 Glotblastn 2390 LYM323oat|10v2|SRR020741S0006351_T1 4221 689 88.31 Glotblastn 2391 LYM323oat|10v2|SRR020741S0011600_T1 4222 689 88.31 Glotblastn 2392 LYM323oat|10v2|SRR020741S0016059_T1 4223 689 88.31 Glotblastn 2393 LYM323oat|10v2|SRR020741S0022525_T1 4224 689 88.31 Glotblastn 2394 LYM323oat|10v2|SRR020741S0028818_T1 4225 689 88.31 Glotblastn 2395 LYM323oat|10v2|SRR020741S0044276_T1 4226 689 88.31 Glotblastn 2396 LYM323oat|10v2|SRR020741S0048787_T1 4226 689 88.31 Glotblastn 2397 LYM323oat|10v2|SRR020741S0127028_T1 4227 689 88.31 Glotblastn 2398 LYM323wheat|10v2|CJ915595_T1 4228 689 88.31 Glotblastn 2399 LYM323barley|gb157SOLEXA|BI952774 4229 689 88.31 Glotblastn 2400 LYM323oat|gb164|CN817388 4217 689 88.31 Glotblastn 2401 LYM323barley|gb157SOLEXA|BF625183 4230 689 88.31 Glotblastn 2402 LYM323oat|gb164|CN817243 4231 689 88.31 Glotblastn 2403 LYM323oat|10v2|CN817710_T1 4232 689 88.31 Glotblastn 2404 LYM323oat|gb164|CN817436 4233 689 88.31 Glotblastn 2405 LYM323oat|gb164|CN817998 4234 689 88.31 Glotblastn 2406 LYM323oat|gb164|CN817172 4217 689 88.31 Glotblastn 2407 LYM323oat|gb164|CN817167 4217 689 88.31 Glotblastn 2408 LYM323oat|10v2|SRR020741S0001370_T1 4235 689 87.01 glotblastn 2409 LYM323barley|gb157SOLEXA|BF264953 4236 689 87.01 Glotblastn 2410 LYM323oat|10v2|CN817235_P1 4237 689 86.1 Globlastp 2411 LYM323barley|10v2|BI951845_T1 4238 689 85.71 Glotblastn 2412 LYM323oat|10v2|SRR020741S0008834_T1 4239 689 85.71 Glotblastn 2413 LYM323wheat|gb164|CA600933 4207 689 85.71 Glotblastn 2414 LYM323wheat|gb164|CK152475 4240 689 85.71 Glotblastn 2415 LYM323wheat|gb164|CA679683 4241 689 84.42 Glotblastn 2416 LYM323wheat|gb164|CK213116 4242 689 83.12 Glotblastn 2417 LYM323wheat|gb164|CK214032 4243 689 83.12 Glotblastn 2418 LYM323wheat|gb164|CA607800 4244 689 81.93 Glotblastn 2419 LYM323wheat|gb164|CK211860 4245 689 81.82 Glotblastn 2420 LYM323barley|gb157SOLEXA|BI951845 4246 689 80.52 Glotblastn 2421 LYM323barley|gb157SOLEXA|BF065474 4247 689 80.52 Glotblastn 2422 LYM323lolium|09v1|AU246702 4248 689 80.5 Globlastp 2423 LYM336wheat|10v2|CA623592_P1 4249 691 87.9 Globlastp 2424 LYM336wheat|gb164|CA623592 4250 691 86.9 Globlastp 2425 LYM336brachypodium|09v1|DV480139_T1 4251 691 86.15 Glotblastn 2426 LYM345cotton|10v2|CO079665_T1 4252 696 86.34 Glotblastn 2427 LYM345cassava|09v1|DB934296_T1 4253 696 80.43 Glotblastn 2428 LYM345poplar|10v1|DE875465_T1 4254 696 80.12 Glotblastn 2429 LYM345poplar|gb170|DB875465 4255 696 80.12 Glotblastn 2430 LYM357sorghum|09v1|SB09G028110 4256 699 93.6 Globlastp 2431 LYM357switchgrass|gb167|FE606773 4257 699 84.7 Globlastp 2432 LYM357millet|10v1|EVO454PM006647_P1 4258 699 84.5 Globlastp 2433 LYM360foxtail_millet|10v2|FXTRMSLX00107249D2_P1 4259 700 89.1 Globlastp 2434LYM360 sugarcane|gb157.3|CA075955 4260 700 84.15 Glotblastn 2435 LYM360wheat|10v2|BF201212_T1 4261 700 83.17 Glotblastn 2436 LYM360brachypodium|09v1|DV476893_T1 4262 700 82.54 Glotblastn 2437 LYM374sugarcane|gb157.3|CA074001 4263 704 92.75 Glotblastn 2438 LYM374maize|gb170|AI855357 4264 704 90.1 globlastp 2439 LYM374sugarcane|10v1|CA154822_T1 4265 704 83.94 Glotblastn 2440 LYM374switchgrass|gb167|FL800516 4266 704 83.51 Glotblastn 2441 LYM374cenchrus|gb166|EB656749_T1 4267 704 80.71 Glotblastn 2442 LYM386sorghum|09v1|SB02G035320 4268 707 83.3 Globlastp 2443 LYM409barley|10v2|BJ446916_P1 4269 710 81.7 Globlastp 2444 LYM409wheat|10v2|BE428448_P1 4270 710 81.5 Globlastp 2445 LYM409brachypodium|09v1|GT770696_P1 4271 710 81.5 Globlastp 2446 LYM409oat|10v2|GR318556_T1 4272 710 80.45 Glotblastn 2447 LYM421sugarcane|10v1|CA133760_P1 4273 713 81.8 Globlastp 2448 LYM421sugarcane|gb157.3|CA133760 4273 713 81.8 Globlastp 2449 LYM421switchgrass|gb167|FL728344 4274 713 80 Globlastp 2450 LYM427sorghum|09v1|CN129490 4275 715 94.78 Glotblastn 2451 LYM440foxtail_millet|10v2|OXEC613292T1_P1 4277 718 84.5 Globlastp 2452 LYM440oat|10v2|CN817360_P1 4278 718 84.1 Globlastp 2453 LYM440brachypodium|09v1|DV470928_P1 4279 718 84.1 Globlastp 2454 LYM440switchgrass|gb167|FE600332 4280 718 84 Globlastp 2455 LYM440wheat|gb164|BE585654 4281 718 82 Globlastp 2456 LYM440wheat|10v2|BE425246_P1 4282 718 81.6 Globlastp 2457 LYM440pseudoroegneria|gb167|FF344793 4283 718 81.6 Globlastp 2458 LYM440wheat|gb164|BE425246 4282 718 81.6 Globlastp 2459 LYM440leymus|gb166|CD808992_P1 4284 718 81.6 Globlastp 2460 LYM440barley|10v2|AV835151_P1 4285 718 81.3 Globlastp 2461 LYM440barley|gb157SOLEXA|AV835151 4285 718 81.3 Globlastp 2462 LYM440millet|09v1|EVO454PM003935 4286 718 80.8 Globlastp 2463 LYM460maize|10v1|CO524622_P1 4287 720 80.1 Globlastp 2464 LYM460maize|gb170|CO524622 4287 720 80.1 Globlastp 2465 LYM465maize|10v1|CD970702_P1 4288 721 87.6 Globlastp 2466 LYM465maize|gb170|CD970702 4289 721 87.2 Globlastp 2467 LYM467rice|gb170|OS01G69920 4290 722 83.96 glotblastn 2468 LYM467brachypodium|09v1|DV471951_T1 4291 722 81.95 Glotblastn 2469 LYM467millet|10v1|CD726346_P1 4292 722 81.6 Globlastp 2470 LYM479maize|gb170|BE345370 4293 725 85.87 Glotblastn 2471 LYM479sugarcane|gb157.3|CA070878 4294 725 85.2 Globlastp 2472 LYM479maize|10v1|BE345370_P1 4295 725 81.9 Globlastp 2473 LYM479millet|10v1|EVO454PM010588_T1 4296 725 80.34 Glotblastn 2474 LYM483sorghum|09v1|SB05G018540 4297 726 80.28 Glotblastn 2475 LYM484sorghum|09v1|SBGWP067232 4298 121 88.84 Glotblastn 2476 LYM305wheat|10v2|CD870432_P1 4299 729 95.8 Globlastp 2477 LYM305brachypodium|09v1|SRR031795S0008555_P1 4300 729 88.4 Globlastp 2478LYM305 rice|gb170|OS02G56310_P1 4301 729 80.8 Globlastp 2479 LYM320wheat|gb164|BE489120 4302 731 97.14 Glotblastn 2480 LYM320wheat|10v2|BE404444_T1 4303 731 97.14 Glotblastn 2481 LYM320wheat|gb164|BE426702 4304 731 95.71 Glotblastn 2482 LYM320wheat|gb164|BE404444 4305 731 95.71 Glotblastn 2483 LYM320brachypodium|09v1|CRPBD004741_T1 4306 731 84.29 Glotblastn 2484 LYM321wheat|10v2|BF292772_P1 4307 732 99.7 Globlastp 2485 LYM321wheat|10v2|AL826398_P1 4308 732 99.3 Globlastp 2486 LYM321wheat|gb164|BE400505 4308 732 99.3 Globlastp 2487 LYM321wheat|10v2|BE406477_P1 4309 732 99.2 Globlastp 2488 LYM321wheat|10v2|BF293528_P1 4310 732 99.2 Globlastp 2489 LYM321barley|10v2|AV922200_P1 4311 732 99 Globlastp 2490 LYM321oat|10v2|CN819912_P1 4312 732 98.4 Globlastp 2491 LYM321brachypodium|09v1|DV480013_P1 4313 732 98.2 Globlastp 2492 LYM321fescue|gb161|DT680639_P1 4314 732 98 Globlastp 2493 LYM321millet|09v1|EVO454PM006280 4315 732 96.7 Globlastp 2494 LYM321millet|10v1|EVO454PM007156_P1 4315 732 96.7 Globlastp 2495 LYM321switchgrass|gb167|FE634889 4316 732 96.7 Globlastp 2496 LYM321rice|gb170|OS01G19450 4317 732 96.5 Globlastp 2497 LYM321foxtail_millet|10v2|OXFXTRMSLX00035855D1T1_P1 4318 732 96.2 globlastp2498 LYM321 sorghum|09v1|SB03G012420 4319 732 96.2 Globlastp 2499 LYM321sugarcane|10v1|CA069900_P1 4320 732 95.6 Globlastp 2500 LYM321sugarcane|gb157.3|CA069900 4320 732 95.6 Globlastp 2501 LYM321pigeonpea|10v1|SRR054580S0025411_P1 4321 732 93.6 Globlastp 2502 LYM321citrus|gb166|CD574299_P1 4322 732 93.3 Globlastp 2503 LYM321soybean|11v1|GLYMA15G15020_P1 4323 732 92.6 Globlastp 2504 LYM321soybean|gb168|BE316107 4323 732 92.6 Globlastp 2505 LYM321cacao|10v1|CU481185_P1 4324 732 92.4 Globlastp 2506 LYM321cassava|09v1|CK645760_P1 4325 732 92.4 Globlastp 2507 LYM321cotton|10v2|BG444263_P1 4326 732 92.4 Globlastp 2508 LYM321cotton|gb164|AI726866 4327 732 92.4 Globlastp 2509 LYM321arabidopsis_lyrata|09v1|JGIAL009077_P1 4328 732 92.3 Globlastp 2510LYM321 soybean|gb168|AW685462 4329 732 92.3 Globlastp 2511 LYM321cowpea|gb166|FF391707_P1 4330 732 92.3 Globlastp 2512 LYM321lotus|09v1|AW719405_P1 4331 732 92.1 Globlastp 2513 LYM321cotton|gb164|AI054464 4332 732 92.1 Globlastp 2514 LYM321papaya|gb165|AM903596_P1 4333 732 92.1 Globlastp 2515 LYM321cucumber|09v1|DV632339_P1 4334 732 92.1 Globlastp 2516 LYM321castorbean|09v1|EG657629_T1 4335 732 91.95 Glotblastn 2517 LYM321cassava|09v1|DV451519_P1 4336 732 91.9 Globlastp 2518 LYM321liquorice|gb171|EF571302_P1 4337 732 91.9 Globlastp 2519 LYM321soybean|11v1|GLYMA07G36840_P1 4338 732 91.9 Globlastp 2520 LYM321soybean|gb168|AW719405 4338 732 91.9 Globlastp 2521 LYM321arabidopsis|10v1|AT5G49460_P1 4339 732 91.9 Globlastp 2522 LYM321arabidopsis|10v1|AT3G06650_P1 4340 732 91.9 Globlastp 2523 LYM321orobanche|10v1|SRR023189S0004714_P1 4341 732 91.8 globlastp 2524 LYM321poplar|10v1|AI164251_P1 4342 732 91.8 Globlastp 2525 LYM321arabidopsis_lyrata|09v1|JGIAL029318_P1 4343 732 91.8 Globlastp 2526LYM321 canola|10v1|CD816535_P1 4344 732 91.8 Globlastp 2527 LYM321canola|gb161|CX193985 4345 732 91.8 Globlastp 2528 LYM321oak|10v1|DN950375_P1 4346 732 91.8 Globlastp 2529 LYM321chestnut|gb170|SRR006295S0000953_T1 4347 732 91.61 Glotblastn 2530LYM321 prunus|10v1|BU046198_P1 4348 732 91.6 Globlastp 2531 LYM321sunflower|10v1|CX946588_P1 4349 732 91.4 Globlastp 2532 LYM321sunflower|gb162|CX946588 4350 732 91.4 Globlastp 2533 LYM321soybean|11v1|GLYMA17G03700_P1 4351 732 91.3 Globlastp 2534 LYM321melon|10v1|AM733929_T1 4352 732 91.12 Glotblastn 2535 LYM321grape|gb160|BQ800086_P1 4353 732 91.1 Globlastp 2536 LYM321solanum_phureja|09v1|SPHBG128839 4354 732 91 Globlastp 2537 LYM321soybean|gb168|DY632707 4355 732 91 Globlastp 2538 LYM321medicago|09v1|AW685462_P1 4356 732 91 Globlastp 2539 LYM321tomato|09v1|BG128839 4357 732 91 Globlastp 2540 LYM321tomato|09v1|BG127685 4358 732 90.8 Globlastp 2541 LYM321pepper|gb171|AF290958_P1 4359 732 90.8 Globlastp 2542 LYM321potato|10v1|BI407063_P1 4360 732 90.8 Globlastp 2543 LYM321potato|gb157.2|BI407063 4360 732 90.8 Globlastp 2544 LYM321peanut|gb171|EH043879 4361 732 90.79 Glotblastn 2545 LYM321orobanche|10v1|SRR023189S0029113_P1 4362 732 90.6 Globlastp 2546 LYM321podocarpus|10v1|SRR065014S0004936_P1 4363 732 90.6 Globlastp 2547 LYM321solanum_phureja|09v1|SPHBG127685 4364 732 90.6 Globlastp 2548 LYM321cucumber|09v1|AM733929_P1 4365 732 90.6 Globlastp 2549 LYM321strawberry|11v1|CO380638_P1 4366 732 90.5 Globlastp 2550 LYM321aquilegia|10v2|DR918406_P1 4367 732 90.5 Globlastp 2551 LYM321aquilegia|gb157.3|DR918406 4367 732 90.5 globlastp 2552 LYM321tobacco|gb162|AJ344605 4368 732 90.46 Glotblastn 2553 LYM321cacao|10v1|CA794428_P1 4369 732 90.3 Globlastp 2554 LYM321nasturtium|10v1|SRR032558S0008004_P1 4370 732 90.3 Globlastp 2555 LYM321pseudotsuga|10v1|SRR065119S0012390_P1 4371 732 90.3 Globlastp 2556LYM321 triphysaria|10v1|BM356770_P1 4372 732 90.3 Globlastp 2557 LYM321triphysaria|gb164|BM356770 4372 732 90.3 Globlastp 2558 LYM321prunus|gb167|BU046198 4373 732 90.13 Glotblastn 2559 LYM321pine|10v2|AW056672_P1 4374 732 90 Globlastp 2560 LYM321zostera|10v1|SRR057351S0000267_P1 4375 732 90 Globlastp 2561 LYM321pine|gb157.2|AW056672 4376 732 90 Globlastp 2562 LYM321sunflower|gb162|BG874299 4377 732 90 Globlastp 2563 LYM321sunflower|10v1|BG874299_P1 4378 732 89.8 Globlastp 2564 LYM321apple|gb171|CN492537_P1 4379 732 89.8 Globlastp 2565 LYM321centaurea|gb166|EL932820_T1 4380 732 89.64 Glotblastn 2566 LYM321artemisia|10v1|EY039774_P1 4381 732 89.6 Globlastp 2567 LYM321spruce|gb162|CO217937 4382 732 89.6 Globlastp 2568 LYM321poplar|gb170|AI164251 4383 732 89.6 Globlastp 2569 LYM321sequoia|10v1|SRR065044S0000472_P1 4384 732 89.5 Globlastp 2570 LYM321cichorium|gb171|DT211033_P1 4385 732 89.5 Globlastp 2571 LYM321centaurea|gb166|EH715001_T1 4386 732 89.47 Glotblastn 2572 LYM321cynara|gb167|GE577055_T1 4387 732 89.31 Glotblastn 2573 LYM321gnetum|10v1|CB080847_P1 4388 732 89.3 Globlastp 2574 LYM321monkeyflower|09v1|DV209951 4389 732 89.3 Globlastp 2575 LYM321monkeyflower|10v1|DV209951_P1 4389 732 89.3 Globlastp 2576 LYM321lettuce|gb157.2|DW104577 4390 732 89.1 Globlastp 2577 LYM321monkeyflower|10v1|DV206182_P1 4391 732 89.1 globlastp 2578 LYM321lettuce|10v1|DW051369_P1 4390 732 89.1 globlastp 2579 LYM321taxus|10v1|SRR032523S0004931_T1 4392 732 88.98 glotblastn 2580 LYM321monkeyflower|09v1|DV206182 4393 732 88.98 glotblastn 2581 LYM321poplar|10v1|CV239972_P1 4394 732 88.6 globlastp 2582 LYM321poplar|gb170|CV239972 4395 732 88.6 globlastp 2583 LYM321aristolochia|10v1|SRR039086S0168375_T1 4396 732 87.68 glotblastn 2584LYM321 artemisia|10v1|EY049275_T1 4397 732 87.17 glotblastn 2585 LYM321lettuce|gb157.2|DW051369 4398 732 85.2 globlastp 2586 LYM321physcomitrella|10v1|BJ158308_P1 4399 732 85.1 globlastp 2587 LYM321ceratodon|10v1|SRR074890S0041190_P1 4400 732 84.9 globlastp 2588 LYM321physcomitrella|10v1|BI436732_P1 4401 732 83.9 globlastp 2589 LYM321safflower|gb162|EL377310 4402 732 83.55 glotblastn 2590 LYM321maize|gb170|LLBG549613 4403 732 82.7 globlastp 2591 LYM321oak|gb170|DN950375 4404 732 81.6 globlastp 2592 LYM322wheat|10v2|BE404343_P1 4405 733 96.6 globlastp 2593 LYM322wheat|gb164|BE404343 4405 733 96.6 globlastp 2594 LYM322brachypodium|09v1|DV489083_P1 4406 733 92.2 globlastp 2595 LYM322rice|gb170|OS02G42520T2 4407 733 88.8 globlastp 2596 LYM323barley|10v2|BI953348_T1 4408 734 98.85 glotblastn 2597 LYM323wheat|gb164|BE213407 4409 734 98.3 globlastp 2598 LYM323wheat|gb164|BE489914 4410 734 98.3 globlastp 2599 LYM323wheat|10v2|BF482226_P1 4411 734 97.7 globlastp 2600 LYM323wheat|10v2|GFXAB020946X1_P1 4411 734 97.7 globlastp 2601 LYM323wheat|gb164|BE213240 4412 734 97.7 globlastp 2602 LYM323maize|gb170|LLDQ245093 4412 734 97.7 globlastp 2603 LYM323pseudoroegneria|gb167|FF353666 4412 734 97.7 globlastp 2604 LYM323wheat|gb164|BE213401 4413 734 97.7 globlastp 2605 LYM323barley|10v2|BI951458_P1 4414 734 97.1 globlastp 2606 LYM323barley|10v2|BI956160_P1 4414 734 97.1 globlastp 2607 LYM323wheat|10v2|GFXAB020926X1_P1 4415 734 97.1 globlastp 2608 LYM323wheat|10v2|GFXAB042065X1_P1 4416 734 97.1 globlastp 2609 LYM323wheat|10v2|GFXWHTRUBIAAX1_P1 4416 734 97.1 globlastp 2610 LYM323wheat|gb164|BE216925 4417 734 96.6 globlastp 2611 LYM323wheat|10v2|GFXAB020956X1_P1 4417 734 96.6 globlastp 2612 LYM323leymus|gb166|EG382657_P1 4418 734 96 globlastp 2613 LYM323wheat|gb164|BE213269 4419 734 96 globlastp 2614 LYM323wheat|gb164|BE418127 4420 734 96 globlastp 2615 LYM323wheat|10v2|BF291663_P1 4420 734 96 globlastp 2616 LYM323wheat|10v2|BF293094_P1 4421 734 95.4 globlastp 2617 LYM323wheat|10v2|BF293721_P1 4422 734 95.4 globlastp 2618 LYM323wheat|10v2|CA635260_P1 4423 734 95.4 globlastp 2619 LYM323wheat|10v2|GFXAB020932X1_P1 4424 734 95.4 globlastp 2620 LYM323wheat|10v2|CA671861_P1 4423 734 95.4 globlastp 2621 LYM323wheat|gb164|AL825247 4425 734 95.4 globlastp 2622 LYM323wheat|gb164|AL827502 4426 734 95.4 globlastp 2623 LYM323wheat|gb164|BE213286 4427 734 95.4 globlastp 2624 LYM323wheat|10v2|CA683563_P1 4427 734 95.4 globlastp 2625 LYM323wheat|10v2|CA731726_P1 4428 734 94.9 globlastp 2626 LYM323wheat|gb164|BE213546 4429 734 94.9 globlastp 2627 LYM323rye|gb164|BE705092 4430 734 94.9 globlastp 2628 LYM323wheat|gb164|BE213432 4431 734 94.9 globlastp 2629 LYM323wheat|10v2|CA722290_P1 4432 734 94.8 globlastp 2630 LYM323barley|10v2|AJ228934_P1 4433 734 94.3 globlastp 2631 LYM323barley|10v2|AV832438_P1 4433 734 94.3 globlastp 2632 LYM323barley|10v2|AV922102_P1 4433 734 94.3 globlastp 2633 LYM323barley|10v2|BE411188_P1 4433 734 94.3 globlastp 2634 LYM323wheat|10v2|BE213432_P1 4434 734 94.3 globlastp 2635 LYM323leymus|gb166|CD808613_P1 4435 734 94.3 globlastp 2636 LYM323cotton|gb164|BF277368 4433 734 94.3 globlastp 2637 LYM323rye|gb164|BE493853 4436 734 94.3 globlastp 2638 LYM323foxtail_millet|10v2|FXTSLX00054363_P1 4437 734 93.7 globlastp 2639LYM323 wheat|10v2|BE401540_P1 4438 734 93.7 globlastp 2640 LYM323wheat|10v2|BE401556_P1 4438 734 93.7 globlastp 2641 LYM323wheat|10v2|BE417960_P1 4438 734 93.7 globlastp 2642 LYM323wheat|10v2|BE499537_P1 4438 734 93.7 globlastp 2643 LYM323wheat|10v2|CA598944_P1 4438 734 93.7 globlastp 2644 LYM323wheat|10v2|CA676828_P1 4438 734 93.7 globlastp 2645 LYM323wheat|10v2|CA688017_P1 4438 734 93.7 globlastp 2646 LYM323wheat|10v2|CA688479_P1 4438 734 93.7 globlastp 2647 LYM323wheat|10v2|CA708934_P1 4438 734 93.7 globlastp 2648 LYM323wheat|10v2|CK152475_P1 4438 734 93.7 globlastp 2649 LYM323wheat|10v2|GFXAB042066X1_P1 4438 734 93.7 globlastp 2650 LYM323wheat|10v2|X83095_P1 4438 734 93.7 globlastp 2651 LYM323wheat|10v2|X00234_P1 4438 734 93.7 globlastp 2652 LYM323wheat|gb164|X00234 4438 734 93.7 globlastp 2653 LYM323wheat|gb164|BE213524 4438 734 93.7 globlastp 2654 LYM323leymus|gb166|CD808567_P1 4439 734 93.7 globlastp 2655 LYM323pseudoroegneria|gb167|FF340115 4438 734 93.7 globlastp 2656 LYM323wheat|10v2|BE401484_P1 4438 734 93.7 globlastp 2657 LYM323wheat|gb164|BE213613 4438 734 93.7 globlastp 2658 LYM323pseudoroegneria|gb167|FF343207 4438 734 93.7 globlastp 2659 LYM323rye|gb164|BE493974 4438 734 93.7 globlastp 2660 LYM323wheat|gb164|BE418243 4438 734 93.7 globlastp 2661 LYM323wheat|gb164|BE213400 4438 734 93.7 globlastp 2662 LYM323wheat|10v2|BE591831_P1 4438 734 93.7 globlastp 2663 LYM323wheat|10v2|BF293130_P1 4438 734 93.7 globlastp 2664 LYM323wheat|10v2|BE418758_P1 4438 734 93.7 globlastp 2665 LYM323wheat|10v2|BI750998_T1 4440 734 93.14 glotblastn 2666 LYM323barley|10v2|HVU43493_P1 4441 734 93.1 globlastp 2667 LYM323wheat|10v2|BE430370_P1 4442 734 93.1 globlastp 2668 LYM323wheat|10v2|CA628296_P1 4442 734 93.1 globlastp 2669 LYM323fescue|gb161|CK802562_P1 4443 734 93.1 globlastp 2670 LYM323fescue|gb161|CK802893_P1 4443 734 93.1 globlastp 2671 LYM323wheat|10v2|CK213583_P1 4444 734 92.6 globlastp 2672 LYM323leymus|gb166|EG384080_P1 4445 734 92.6 globlastp 2673 LYM323leymus|gb166|CD808506_P1 4446 734 92.6 globlastp 2674 LYM323fescue|gb161|CK802838_P1 4447 734 92.5 globlastp 2675 LYM323foxtail_millet|10v2|OXFXTSLX00005856T1_T1 4448 734 92 glotblastn 2676LYM323 lolium|10v1|AU246416_P1 4449 734 92 globlastp 2677 LYM323lolium|10v1|AU246718_P1 4450 734 92 globlastp 2678 LYM323lolium|10v1|AU246894_P1 4449 734 92 globlastp 2679 LYM323lolium|10v1|AU246987_P1 4451 734 92 globlastp 2680 LYM323oat|10v2|AF192778XX2_P1 4452 734 92 globlastp 2681 LYM323oat|10v2|GR315764_P1 4452 734 92 globlastp 2682 LYM323oat|10v2|GR319109_P1 4452 734 92 globlastp 2683 LYM323oat|10v2|GR322170_P1 4452 734 92 globlastp 2684 LYM323oat|10v2|GR330123_P1 4452 734 92 globlastp 2685 LYM323oat|10v2|SRR020741S0011514.P1 4452 734 92 globlastp 2686 LYM323wheat|10v2|GFXAB042064X1_T1 4453 734 92 glotblastn 2687 LYM323leymus|gb166|CD808636_P1 4454 734 92 globlastp 2688 LYM323fescue|gb161|CK802053_P1 4455 734 92 globlastp 2689 LYM323lolium|09v1|AU246467 4456 734 92 globlastp 2690 LYM323lolium|10v1|AU246467_P1 4456 734 92 globlastp 2691 LYM323lolium|09v1|AY693395 4449 734 92 globlastp 2692 LYM323fescue|gb161|DT679236_P1 4450 734 92 globlastp 2693 LYM323oat|10v2|CN817388_P1 4452 734 92 globlastp 2694 LYM323oat|10v2|GR318240_P1 4452 734 92 globlastp 2695 LYM323barley|10v2|BG344511_T1 4457 734 91.95 glotblastn 2696 LYM323pseudoroegneria|gb167|FF350077 4458 734 91.5 globlastp 2697 LYM323wheat|10v2|BE401428_T1 4459 734 91.43 glotblastn 2698 LYM323barley|10v2|BI951695_P1 4460 734 91.4 globlastp 2699 LYM323oat|10v2|CN817197_P1 4461 734 91.4 globlastp 2700 LYM323oat|10v2|CN817540_P1 4461 734 91.4 globlastp 2701 LYM323oat|10v2|GR313438_P1 4461 734 91.4 globlastp 2702 LYM323oat|10v2|GR318094_P1 4462 734 91.4 globlastp 2703 LYM323oat|10v2|GR320691_P1 4463 734 91.4 globlastp 2704 LYM323oat|10v2|SRR020741S0006634_P1 4461 734 91.4 globlastp 2705 LYM323oat|10v2|SRR020741S0070820_P1 4461 734 91.4 globlastp 2706 LYM323fescue|gb161|DT679230_P1 4464 734 91.4 globlastp 2707 LYM323oat|gb164|CN817363 4463 734 91.4 globlastp 2708 LYM323brachypodium|09v1|DV473139_P1 4465 734 91.4 globlastp 2709 LYM323brachypodium|09v1|DV473778_P1 4466 734 91.4 globlastp 2710 LYM323oat|10v2|AF096617XX2_P1 4463 734 91.4 globlastp 2711 LYM323barley|10v2|CA591948_T1 4467 734 91.38 glotblastn 2712 LYM323oat|10v2|GR320859_T1 4468 734 91.38 glotblastn 2713 LYM323oat|10v2|SRR020741S0022596_T1 4469 734 91.38 glotblastn 2714 LYM323barley|10v2|BF622956_P1 4470 734 90.9 globlastp 2715 LYM323leymus|gb166|CD808493_P1 4471 734 90.9 globlastp 2716 LYM323lolium|10v1|AU246624_P1 4472 734 90.8 globlastp 2717 LYM323oat|10v2|AF096617XXl_P1 4473 734 90.8 globlastp 2718 LYM323oat|10v2|GR318091_P1 4474 734 90.8 globlastp 2719 LYM323oat|10v2|GR318615_P1 4475 734 90.8 globlastp 2720 LYM323oat|10v2|GR322397_P1 4476 734 90.8 globlastp 2721 LYM323oat|10v2|GR339642_P1 4477 734 90.8 globlastp 2722 LYM323oat|10v2|SRR020741S0022271_P1 4478 734 90.8 globlastp 2723 LYM323oat|10v2|CN817363_P1 4475 734 90.8 globlastp 2724 LYM323oat|gb164|CN817333 4479 734 90.8 globlastp 2725 LYM323oat|10v2|GR313197_P1 4473 734 90.8 globlastp 2726 LYM323fescue|gb161|DT681296_P1 4480 734 90.8 globlastp 2727 LYM323rye|gb164|GFXAB020942X1 4481 734 90.8 globlastp 2728 LYM323cotton|10v2|BM359089_P1 4482 734 90.5 globlastp 2729 LYM323barley|10v2|BF625183_P1 4483 734 90.3 globlastp 2730 LYM323wheat|10v2|CA660392_T1 4484 734 90.29 glotblastn 2731 LYM323oat|10v2|CN817436_T1 4485 734 90.23 glotblastn 2732 LYM323oat|10v2|CN818311_T1 4486 734 90.23 glotblastn 2733 LYM323oat|10v2|GR331875XX1_T1 4487 734 90.23 glotblastn 2734 LYM323oat|10v2|GR333189_T1 4231 734 90.23 glotblastn 2735 LYM323barley|10v2|BI949564_P1 4488 734 90.2 globlastp 2736 LYM323oat|10v2|GR316246_P1 4489 734 90.2 globlastp 2737 LYM323oat|10v2|GR328381_P1 4490 734 90.2 globlastp 2738 LYM323oat|10v2|GR320823_P1 4491 734 90.2 globlastp 2739 LYM323oat|10v2|CN817268_P1 4492 734 90.2 globlastp 2740 LYM323oat|gb164|CN817197 4492 734 90.2 globlastp 2741 LYM323fescue|gb161|DT679671_P1 4493 734 90.2 globlastp 2742 LYM323oat|10v2|AF192776XX2_P1 4494 734 89.8 globlastp 2743 LYM323oat|10v2|AF104249XX1_P1 4495 734 89.7 globlastp 2744 LYM323oat|10v2|AF104249XX2_P1 4495 734 89.7 globlastp 2745 LYM323oat|10v2|CN817172_P1 4495 734 89.7 globlastp 2746 LYM323oat|10v2|CN817234_P1 4495 734 89.7 globlastp 2747 LYM323oat|10v2|CN817322_P1 4496 734 89.7 globlastp 2748 LYM323oat|10v2|CN817884_P1 4497 734 89.7 globlastp 2749 LYM323oat|10v2|CN817972_P1 4498 734 89.7 globlastp 2750 LYM323oat|10v2|CN818467_P1 4499 734 89.7 globlastp 2751 LYM323oat|10v2|CN818595_P1 4498 734 89.7 globlastp 2752 LYM323oat|10v2|GR313592_P1 4498 734 89.7 globlastp 2753 LYM323oat|10v2|GR316111_P1 4496 734 89.7 globlastp 2754 LYM323oat|10v2|GR318797_P1 4499 734 89.7 globlastp 2755 LYM323oat|10v2|GR319376_P1 4498 734 89.7 globlastp 2756 LYM323oat|10v2|GR331789XX1_P1 4495 734 89.7 globlastp 2757 LYM323oat|10v2|GR338398_P1 4500 734 89.7 globlastp 2758 LYM323oat|10v2|SRR020741S0001757_P1 4495 734 89.7 globlastp 2759 LYM323oat|10v2|SRR020741S0004656_P1 4495 734 89.7 globlastp 2760 LYM323oat|10v2|SRR020741S0007177_P1 4499 734 89.7 globlastp 2761 LYM323oat|10v2|SRR020741S0043377_P1 4498 734 89.7 globlastp 2762 LYM323oat|10v2|SRR020741S0152332_P1 4500 734 89.7 globlastp 2763 LYM323oat|10v2|AF097359XX1_P1 4501 734 89.7 globlastp 2764 LYM323fescue|gb161|CK800817_P1 4502 734 89.7 globlastp 2765 LYM323lolium|09v1|AU246411 4503 734 89.7 globlastp 2766 LYM323lolium|10v1|AU246411_P1 4503 734 89.7 globlastp 2767 LYM323oat|10v2|GR338950_T1 4504 734 89.66 glotblastn 2768 LYM323oat|10v2|GR341316_T1 4505 734 89.66 glotblastn 2769 LYM323oat|10v2|SRR020741S0010662_T1 4506 734 89.14 glotblastn 2770 LYM323cotton|10v2|BG446686_P1 4507 734 89.1 globlastp 2771 LYM323oat|10v2|AF097359XX2_P1 4508 734 89.1 globlastp 2772 LYM323oat|10v2|GR319246XX1_P1 4509 734 89.1 globlastp 2773 LYM323oat|10v2|GR319286_P1 4510 734 89.1 globlastp 2774 LYM323oat|10v2|SRR020741S0010488_P1 4510 734 89.1 globlastp 2775 LYM323oat|10v2|GFXAF200303X1_T1 4511 734 89.08 glotblastn 2776 LYM323oat|10v2|GO597200_T1 4512 734 89.08 glotblastn 2777 LYM323oat|10v2|SRR020741S0038759_T1 4231 734 89.08 glotblastn 2778 LYM323wheat|gb164|BE425600 4513 734 88.6 globlastp 2779 LYM323brachypodium|09v1|DV473211_P1 4514 734 88.6 globlastp 2780 LYM323oat|gb164|CN817234 4515 734 88.6 globlastp 2781 LYM323oat|10v2|SRR020741S0024573_T1 4516 734 88.57 glotblastn 2782 LYM323oat|10v2|SRR020741S0001393_T1 4217 734 88.51 glotblastn 2783 LYM323oat|10v2|SRR020741S0087500_T1 4517 734 88.51 glotblastn 2784 LYM323oat|10v2|AF104250_P1 4518 734 88.5 globlastp 2785 LYM323oat|10v2|GR342208_P1 4519 734 88.5 globlastp 2786 LYM323oat|10v2|SRR020741S0002484_P1 4520 734 88.5 globlastp 2787 LYM323oat|10v2|SRR020741S0007079_P1 4521 734 88.5 globlastp 2788 LYM323barley|10v2|BF624247_P1 4522 734 88 globlastp 2789 LYM323wheat|10v2|CA688552_P1 4523 734 88 globlastp 2790 LYM323wheat|gb164|CK216687 4524 734 88 globlastp 2791 LYM323oat|10v2|AF097360_P1 4525 734 87.9 globlastp 2792 LYM323oat|gb164|CN817884 4526 734 87.9 globlastp 2793 LYM323wheat|gb164|DR737649 4527 734 87.9 globlastp 2794 LYM323foxtail_millet|10v2|OXFXTSLX00013814T1_T1 4528 734 87.43 glotblastn 2795LYM323 wheat|10v2|CK213487_T1 4529 734 87.43 glotblastn 2795 LYM323wheat|gb164|CK213487 4530 734 87.43 glotblastn 2796 LYM323foxtail_millet|10v2|FXTSLX00047355_P1 4531 734 87.4 globlastp 2797LYM323 oat|10v2|SRR020741S0000730_P1 4532 734 87.4 globlastp 2798 LYM323oat|10v2|SRR020741S0009815_P1 4533 734 86.8 globlastp 2799 LYM323oat|10v2|CN817273_P1 4534 734 86.8 globlastp 2800 LYM323oat|10v2|GR337704_T1 4535 734 86.44 glotblastn 2801 LYM323brachypodium|09v1|SRR031799S0026606_P1 4536 734 86.4 globlastp 2802LYM323 barley|10v2|BE411629_P1 4537 734 86.3 globlastp 2803 LYM323barley|10v2|BI954460_T1 4538 734 86.29 glotblastn 2804 LYM323oat|gb164|CN817235 4539 734 85.6 globlastp 2805 LYM323oat|10v2|AF192773XX2_P1 4540 734 85.2 globlastp 2806 LYM323rye|gb164|BE494450 4541 734 85.2 globlastp 2807 LYM323barley|10v2|BE411336_P1 4542 734 85.1 globlastp 2808 LYM323foxtail_millet|10v2|FXTSLX00047015_P1 4543 734 84.6 globlastp 2809LYM323 rice|gb170|AA753240_P1 4544 734 84.6 globlastp 2810 LYM323wheat|10v2|CA484878_P1 4544 734 84.6 globlastp 2811 LYM323rice|gb170|OS12G19470_P1 4545 734 84 globlastp 2812 LYM323oat|10v2|SRR020741S0016505_P1 4546 734 83.5 globlastp 2813 LYM323rice|gb170|OS12G17600_P1 4547 734 83.4 globlastp 2814 LYM323rice|gb170|U38156_P1 4548 734 83.4 globlastp 2815 LYM323oat|10v2|SRR020741S0031808_P1 4549 734 83.3 globlastp 2816 LYM323oat|gb164|CN817273 4550 734 82.8 globlastp 2817 LYM323wheat|10v2|DR737558_T1 4551 734 82.29 glotblastn 2818 LYM323oat|10v2|GR320006_P1 4552 734 81.8 globlastp 2819 LYM323oat|10v2|SRR020741S0039481_P1 4553 734 81.6 globlastp 2820 LYM323oat|10v2|SRR020741S0063119_P1 4554 734 81.6 globlastp 2821 LYM323oat|10v2|CN817333_P1 4555 734 81.6 globlastp 2822 LYM323lovegrass|gb167|EH187915_P1 4556 734 80 globlastp 2823 LYM323lovegrass|gb167|EH188588_P1 4557 734 80 globlastp 2824 LYM327wheat|10v2|BQ484093_P1 4558 736 90.3 globlastp 2825 LYM327wheat|gb164|BQ484093 4558 736 90.3 globlastp 2826 LYM327wheat|10v2|BE443815_P1 4559 736 89.2 globlastp 2827 LYM327wheat|gb164|BE443815 4559 736 89.2 globlastp 2828 LYM328wheat|gb164|BE429282 4560 737 88.71 glotblastn 2829 LYM328wheat|gb164|BG904264 4561 737 85.48 glotblastn 2830 LYM328wheat|10v2|CJ963697_P1 4562 737 84.7 globlastp 2831 LYM328wheat|10v2|AJ603094_P1 4563 737 83.9 globlastp 2832 LYM328wheat|gb164|BE422922 4564 737 83.87 glotblastn 2833 LYM329wheat|gb164|BE497427 4565 738 81.75 glotblastn 2834 LYM329wheat|10v2|BE497427_P1 4566 738 81.4 globlastp 2835 LYM331wheat|gb164|BE213319 4567 740 96.08 glotblastn 2836 LYM331oat|10v2|GR319589_P1 4568 740 88.6 globlastp 2837 LYM331switchgrass|gb167|FE635405_T1 4569 740 80 glotblastn 2838 LYM344cacao|10v1|CU480734_P1 4570 746 86.9 globlastp 2839 LYM344cacao|gb167|CU480734 4570 746 86.9 globlastp 2840 LYM346sugarcane|10v1|CA073967_P1 4571 748 93.4 globlastp 2841 LYM346sugarcane|gb157.3|CA073967 4571 748 93.4 globlastp 2842 LYM346sorghum|09v1|SB07G025410 4572 748 87.3 globlastp 2843 LYM346wheat|10v2|BQ838271_P1 4573 748 83.1 globlastp 2844 LYM346barley|10v2|AW983456_P1 4574 748 83.1 globlastp 2845 LYM346barley|gb157SOLEXA|AL507138 4574 748 83.1 globlastp 2846 LYM346wheat|10v2|BF483471_P1 4573 748 83.1 globlastp 2847 LYM346wheat|gb164|BF483471 4573 748 83.1 globlastp 2848 LYM346millet|10v1|PMSLX0018164D1_P1 4575 748 82 globlastp 2849 LYM353sorghum|09v1|SB01G046480 4576 750 92.41 glotblastn 2850 LYM355sorghum|09v1|SB04G021010 4577 752 89.4 globlastp 2851 LYM355sugarcane|10v1|CA101409_P1 4578 752 88.9 globlastp 2852 LYM355sugarcane|gb157.3|CA066011 4578 752 88.9 globlastp 2853 LYM355switchgrass|gb167|FL759335 4579 752 87 globlastp 2854 LYM355brachypodium|09v1|DV469589_P1 4580 752 80.4 globlastp 2855 LYM355rice|gb170|OS02G31030 4581 752 80.3 globlastp 2856 LYM355switchgrass|gb167|FL773247_P1 4582 752 80.1 globlastp 2857 LYM363sorghum|09v1|SB02G040470 4583 753 95.7 globlastp 2858 LYM363millet|10v1|EVO454PM003032_T1 4584 753 89.69 glotblastn 2859 LYM363foxtail_millet|10v2|FXTRMSLX00804145D1_P1 4585 753 87.2 globlastp 2860LYM363 brachypodium|09v1|GT760454_P1 4586 753 86.1 globlastp 2861 LYM363rice|gb170|OS07G44070 4587 753 86 globlastp 2862 LYM363wheat|gb164|BE419569 4588 753 83.86 glotblastn 2863 LYM363wheat|10v2|BE419569_P1 4589 753 83.7 globlastp 2864 LYM363switchgrass|gb167|FE647044 4590 753 80.6 globlastp 2865 LYM366wheat|10v2|CJ551040_P1 4591 755 83.3 globlastp 2866 LYM366wheat|gb164|CJ551040 4591 755 83.3 globlastp 2867 LYM366wheat|gb164|CJ580047 4592 755 81.2 globlastp 2868 LYM366wheat|10v2|CJ544810_P1 4593 755 80.6 globlastp 2869 LYM367sorghum|09v1|SB01G045280 4594 756 93.3 globlastp 2870 LYM367brachypodium|09v1|SRR031795S0020888_P1 4595 756 83.4 globlastp 2871LYM367 rice|gb170|OS03G08140 4596 756 83.1 globlastp 2872 LYM367switchgrass|gb167|FL706891 4597 756 82.33 glotblastn 2873 LYM367wheat|10v2|BG262395_P1 4598 756 80.8 globlastp 2874 LYM369sugarcane|10v1|CA100818_T1 4599 757 89.67 glotblastn 2875 LYM369sugarcane|gb157.3|CA100818 4600 757 89.67 glotblastn 2876 LYM369millet|09v1|EVO454PM009868 4601 757 89.3 globlastp 2877 LYM369millet|10v1|EVO454PM009868_P1 4601 757 89.3 globlastp 2878 LYM369barley|gb157SOLEXA|BE411508 4602 757 82.23 glotblastn 2879 LYM369wheat|gb164|BF203113 4603 757 82.23 glotblastn 2880 LYM369wheat|gb164|BE419437 4604 757 81.82 glotblastn 2881 LYM369wheat|10v2|BE419437_T1 4605 757 81.82 glotblastn 2882 LYM369fescue|gb161|CK802835_T1 4606 757 81.4 glotblastn 2883 LYM372sorghum|09v1|SB04G024660 4607 760 87.1 globlastp 2884 LYM372sugarcane|10v1|BQ535890_P1 4608 760 86 globlastp 2885 LYM372sugarcane|gb157.3|BQ535890 4609 760 85.8 globlastp 2886 LYM375sorghum|09v1|SB01G001810 4610 762 88.2 globlastp 2887 LYM375sugarcane|10v1|CA074815_P1 4611 762 84.7 globlastp 2888 LYM375maize|10v1|AI948025_P1 4612 762 84 globlastp 2889 LYM375maize|gb170|AI948025 4612 762 84 globlastp 2890 LYM375sugarcane|gb157.3|CA074815 4613 762 83.9 globlastp 2891 LYM375switchgrass|gb167|FL812389 4614 762 83.19 glotblastn 2892 LYM385maize|10v1|ZMCRP2V208186_P1 4615 765 90.8 globlastp 2893 LYM385maize|10v1|ZMCRP2VO19939_T1 4616 765 89.15 glotblastn 2894 LYM387maize|10v1|AI665175_P1 4617 766 87.8 globlastp 2895 LYM387maize|gb170|AI665175 4617 766 87.8 globlastp 2896 LYM387foxtail_millet|10v2|SICRP002515_P1 4618 766 85.5 globlastp 2897 LYM387brachypodium|09v1|SRR031795S0027446_P1 4619 766 82.6 globlastp 2898LYM387 wheat|gb164|BQ236742 4620 766 81.52 glotblastn 2899 LYM387millet|10v1|EVO454PM429706_P1 4621 766 80.9 globlastp 2900 LYM387oat|10v2|GR327792_T1 4622 766 80.9 glotblastn 2901 LYM387barley|10v2|BE454704_T1 4623 766 80.22 glotblastn 2902 LYM387wheat|10v2|BE427516_P1 4624 766 80.1 globlastp 2903 LYM410brachypodium|09v1|GT774325_P1 4625 768 89.6 globlastp 2904 LYM410sorghum|09v1|SB10G025350 4626 768 88.5 globlastp 2905 LYM410maize|10v1|CD946231_P1 4627 768 87.4 globlastp 2906 LYM410maize|10v1|AI855346_P1 4628 768 86.9 globlastp 2907 LYM441maize|10v1|AI734556_P1 4629 771 91 globlastp 2908 LYM441maize|gb170|AI734556 4630 771 90.9 globlastp 2909 LYM445maize|10v1|AA979844_P1 4631 773 87.1 globlastp 2910 LYM445millet|10v1|PMSLX0015877D1_P1 4632 773 83.8 globlastp 2911 LYM445switchgrass|gb167|DN142436_P1 4633 773 82.7 globlastp 2912 LYM463maize|gb170|AI739812 4634 776 95.1 globlastp 2913 LYM463maize|10v1|AI739812_P1 4635 776 95 globlastp 2914 LYM463maize|10v1|AI941779_P1 4636 776 93.6 globlastp 2915 LYM463maize|gb170|AI941779 4636 776 93.6 globlastp 2916 LYM463foxtail_millet|10v2|SICRP008195_T1 4637 776 89.76 glotblastn 2917 LYM463rice|gb170|OS01G56910 4638 776 84.9 globlastp 2918 LYM463brachypodium|09v1|DV473125_P1 4639 776 80.5 globlastp 2919 LYM466maize|10v1|AW000428_P1 4640 778 94.6 globlastp 2920 LYM466maize|gb170|AW000428 4641 778 94.4 globlastp 2921 LYM466maize|gb170|AI783091 4642 778 93.4 globlastp 2922 LYM466maize|10v1|AI783091_P1 4643 778 93 globlastp 2923 LYM466switchgrass|gb167|FL820161 4644 778 91.9 globlastp 2924 LYM466millet|10v1|EVO454PM002550_P1 4645 778 91.1 globlastp 2925 LYM466switchgrass|gb167|FE655508 4646 778 90.9 globlastp 2926 LYM466millet|09v1|EVO454PM002550 4647 778 84.1 globlastp 2927 LYM466barley|10v2|BF623940_P1 4648 778 80.6 globlastp 2928 LYM466wheat|10v2|BE404667_P1 4649 778 80.6 globlastp 2929 LYM466wheat|gb164|BE404667 4649 778 80.6 globlastp 2930 LYM475sugarcane|gb157.3|BQ536199 4650 781 91.8 globlastp 2931 LYM475sugarcane|10v1|BQ536199_P1 4651 781 91.8 globlastp 2932 LYM475sugarcane|gb157.3|CA156864 4652 781 90.25 glotblastn 2933 LYM475maize|10v1|CO445714_P1 4653 781 84.3 globlastp 2934 LYM475maize|10v1|DR823853_P1 4654 781 81.6 globlastp 2935 LYM475maize|10v1|EU956996_P1 4654 781 81.6 globlastp 2936 LYM475millet|10v1|CD724611_P1 4655 781 80.9 globlastp 2937 LYM488maize|10v1|CB334691_P1 4656 784 93.2 globlastp 2938 LYM488maize|gb170|CB334691 4656 784 93.2 globlastp 2939 LYM496sugarcane|10v1|CA081211_P1 4657 786 93.7 globlastp 2940 LYM496maize|10v1|AI600771_P1 4658 786 92.1 globlastp 2941 LYM496maize|gb170|AI600771 4658 786 92.1 globlastp 2942 LYM496switchgrass|gb167|FE633056 4659 786 88.3 globlastp 2943 LYM496sugarcane|gb157.3|CA081211 4660 786 86.49 glotblastn 2944 LYM496millet|10v1|EVO454PM005492_P1 4661 786 86.4 globlastp 2945 LYM496foxtail_millet|10v2|SICRP031778_T1 4662 786 84.17 glotblastn 2946LYM397_H2 maize|10v1|AI372372_T1 4663 792 93.25 glotblastn Table 2:Provided are the homologous polypeptides and polynucleotides of thegenes for increasing yield (e.g., oil yield, seed yield, fiber yieldand/or quality), growth rate, vigor, biomass, abiotic stress tolerance,nitrogen use efficiency, water use efficiency and fertilizer useefficiency genes of a plant which are listed in Table 1 above. Homologywas calculated as % of identity over the aligned sequences. The querysequences were polynucleotide sequences SEQ ID NOs: 1-288 and 289-480;and polypeptide SEQ ID NOs: 481-727, and 728-792 and the subjectsequences are protein sequences identified in the database based ongreater than 80% global identity to the predicted translated sequencesof the query nucleotide sequences or to the polypeptide sequences.Nucl.″ = polynucleotide; “polyp.” = polypeptide; “Algor.” = algorithm(used for sequence alignment and determination of percent homology);“Hom.”—homology; “iden.”—identity.

The output of the functional genomics approach described herein is a setof genes highly predicted to improve yield and/or other agronomicimportant traits such as growth rate, vigor, oil content, fiber yieldand/or quality, biomass, growth rate, abiotic stress tolerance, nitrogenuse efficiency, water use efficiency and fertilizer use efficiency of aplant by increasing their expression. Although each gene is predicted tohave its own impact, modifying the mode of expression of more than onegene is expected to provide an additive or synergistic effect on theplant yield and/or other agronomic important yields performance.Altering the expression of each gene described here alone or set ofgenes together increases the overall yield and/or other agronomicimportant traits, hence expects to increase agricultural productivity.

Example 3 Production of Barley Transcriptome and High ThroughputCorrelation Analysis Using 44K Barley Oligonucleotide Micro-Array

In order to produce a high throughput correlation analysis, the presentinventors utilized a Barley oligonucleotide micro-array, produced byAgilent Technologies [Hypertext Transfer Protocol://World Wide Web (dot)chem. (dot) agilent (dot) com/Scripts/PDS (dot) asp?1Page=50879]. Thearray oligonucleotide represents about 47,500 Barley genes andtranscripts. In order to define correlations between the levels of RNAexpression and yield or vigor related parameters, various plantcharacteristics of 25 different Barley accessions were analyzed. Amongthem, 13 accessions encompassing the observed variance were selected forRNA expression analysis. The correlation between the RNA levels and thecharacterized parameters was analyzed using Pearson correlation test[Hypertext Transfer Protocol://World Wide Web (dot) davidmlane (dot)com/hyperstat/A34739 (dot) html].

Experimental Procedures

Five tissues at different developmental stages [meristem, flower,booting spike, stem, flag leaf], representing different plantcharacteristics, were sampled and RNA was extracted as describedhereinabove under “GENERAL EXPERIMENTAL AND BIOINFORMATICS METHODS”.

For convenience, each micro-array expression information tissue type hasreceived a Set ID as summarized in Table 3 below.

TABLE 3 Barley transcriptome expression sets Expression Set Set IDMeristem A Flower B Booting spike C Stem D Flag leaf E Table 3: Providedare the identification (ID) letters of each of the Barley expressionsets.

Barley Yield Components and Vigor Related Parameters Assessment—

13 Barley accessions in 4 repetitive blocks (named A, B, C, and D), eachcontaining 4 plants per plot were grown at net house. Plants werephenotyped on a daily basis following the standard descriptor of barley(Table 4, below). Harvest was conducted while 50% of the spikes were dryto avoid spontaneous release of the seeds. Plants were separated to thevegetative part and spikes, of them, 5 spikes were threshed (grains wereseparated from the glumes) for additional grain analysis such as sizemeasurement, grain count per spike and grain yield per spike. Allmaterial was oven dried and the seeds were threshed manually from thespikes prior to measurement of the seed characteristics (weight andsize) using scanning and image analysis. The image analysis systemincluded a personal desktop computer (Intel P4 3.0 GHz processor) and apublic domain program—ImageJ 1.37 (Java based image processing program,which was developed at the U.S. National Institutes of Health and freelyavailable on the internet [Hypertext Transfer Protocol://rsbweb (dot)nih (dot) gov/]. Next, analyzed data was saved to text files andprocessed using the JMP statistical analysis software (SAS institute).

TABLE 4 Barley standard descriptors Trait Parameter Range DescriptionGrowth habit Scoring 1-9 Prostrate (1) or Erect (9) Hairiness of ScoringP (Presence)/A (Absence) Absence (1) or Presence (2) basal leaves StemScoring 1-5 Green (1), Basal only or pigmentation Half or more (5) Daysto Days Days from sowing to Flowering emergence of awns Plant heightCentimeter (cm) Height from ground level to top of the longest spikeexcluding awns Spikes per plant Number Terminal Counting Spike lengthCentimeter (cm) Terminal Counting 5 spikes per plant Grains per spikeNumber Terminal Counting 5 spikes per plant Vegetative dry GramOven-dried for 48 hours at weight 70° C. Spikes dry Gram Oven-dried for48 hours at weight 30° C. Table 4.

At the end of the experiment (50% of the spikes were dry) all spikesfrom plots within blocks A-D were collected, and the followingmeasurements were performed:

(i) Grains per spike—The total number of grains from 5 spikes that weremanually threshed was counted. The average grain per spike wascalculated by dividing the total grain number by the number of spikes.

(ii) Grain average size (cm)—The total grains from 5 spikes that weremanually threshed were scanned and images were analyzed using thedigital imaging system. Grain scanning was done using Brother scanner(model DCP-135), at the 200 dpi resolution and analyzed with Image Jsoftware. The average grain size was calculated by dividing the totalgrain size by the total grain number.

(iii) Grain average weight (mgr)—The total grains from 5 spikes thatwere manually threshed were counted and weight. The average weight wascalculated by dividing the total weight by the total grain number.

(iv) Grain yield per spike (gr)—The total grains from 5 spikes that weremanually threshed were weight. The grain yield was calculated bydividing the total weight by the spike number.

(v) Spike length analysis—The five chosen spikes per plant were measuredusing measuring tape excluding the awns.

(vi) Spike number analysis—The spikes per plant were counted.

Additional parameters were measured as follows:

Growth habit scoring—At growth stage 10 (booting), each of the plantswas scored for its growth habit nature. The scale that was used was 1for prostate nature till 9 for erect.

Hairiness of basal leaves—At growth stage 5 (leaf sheath strongly erect;end of tillering), each of the plants was scored for its hairinessnature of the leaf before the last. The scale that was used was 1 forprostate nature till 9 for erect.

Plant height—At harvest stage (50% of spikes were dry), each of theplants was measured for its height using measuring tape. Height wasmeasured from ground level to top of the longest spike excluding awns.

Days to flowering—Each of the plants was monitored for flowering date.Days of flowering was calculated from sowing date till flowering date.

Stem pigmentation—At growth stage 10 (booting), each of the plants wasscored for its stem color. The scale that was used was 1 for green till5 for full purple.

Vegetative dry weight and spike yield—At the end of the experiment (50%of the spikes were dry) all spikes and vegetative material from plotswithin blocks A-D are collected. The biomass and spikes weight of eachplot was separated, measured and divided by the number of plants.

Dry weight=total weight of the vegetative portion above ground(excluding roots) after drying at 70° C. in oven for 48 hours;

Spike yield per plant=total spike weight per plant (gr) after drying at30° C. in oven for 48 hours.

Harvest Index (for barley)—The harvest index is calculated using FormulaV.

Formula V: Harvest Index=Average spike dry weight per plant/(Averagevegetative dry weight per plant+Average spike dry weight per plant)

TABLE 5 Barley correlated parameters (vectors) Corre- Correlatedparameter with (units) lation Id Grains per spike (numbers) 1 Grainssize (mm²) 2 Grain weight (miligrams) 3 Grain Yield per spike (gr/spike)4 Spike length (cm) 5 Spikes per plant (numbers) 6 Growth habit (scores1-9) 7 Hairiness of basal leaves (scoring 1-2) 8 Plant height (cm) 9Days to flowering (days) 10 Stem pigmentation (scoring 1-5) 11Vegetative dry weight (gram) 12 Harvest Index (ratio) 13 Table 5.Provided are the Barley correlated parameters (vectors).

Experimental Results

13 different Barley accessions were grown and characterized for 13parameters as described above. The average for each of the measuredparameter was calculated using the JMP software and values aresummarized in Tables 6 and 7 below. Subsequent correlation analysisbetween the various transcriptome expression sets (Table 3) and theaverage parameters, was conducted. Follow, results were integrated tothe database (Table 8 below).

TABLE 6 Measured parameters of correlation Ids in Barley accessionsParameter Spikes Days to Grain Spike Grains Grains Growth Accession perplant flowering weight length Size per spike habit Amatzya 48.85 62.4035.05 12.04 0.27 20.23 2.60 Ashqelon 48.27 64.08 28.06 10.93 0.23 17.982.00 Canada_park 37.42 65.15 28.76 11.83 0.24 17.27 1.92 Havarim_stream61.92 58.92 17.87 9.90 0.17 17.73 3.17 Jordan_est 33.27 63.00 41.2211.68 0.29 14.47 4.33 Klil 41.69 70.54 29.73 11.53 0.28 16.78 2.69Maale_Efraim ND 52.80 25.22 8.86 0.22 13.47 3.60 Mt_Arbel 40.63 60.8834.99 11.22 0.28 14.07 3.50 Mt_Harif 62.00 58.10 20.58 11.11 0.19 21.543.00 Neomi 49.33 53.00 27.50 8.58 0.22 12.10 3.67 Neot_Kdumim 50.6060.40 37.13 10.18 0.27 14.36 2.47 Oren_canyon 43.09 64.58 29.56 10.510.27 15.28 3.50 Yeruham 51.40 56.00 19.58 9.80 0.18 17.07 3.00 Table 6.Provided are the values of each of the parameters measured in Barleyaccessions according to the following correlation identifications(Correlation Ids): 6 = Spikes per plant; 10 = Days to flowering; 3 =Grain weight; 5 = Spike length; 2 = Grains Size; 1 = Grains per spike; 7= Growth habit.

TABLE 7 Barley accessions, additional measured parameters ParameterHairiness of basal Plant Grain yield Stem Vegetative Harvest Accessionleaves height per spike pigmentation dry weight Index Amatzya 1.53134.27 3.56 1.13 78.87 0.45 Ashqelon 1.33 130.50 2.54 2.50 66.14 0.42Canada_park 1.69 138.77 2.58 1.69 68.49 0.40 Havarim_stream 1.08 114.581.57 1.75 53.39 0.44 Jordan_est 1.42 127.75 3.03 2.33 68.30 0.43 Klil1.69 129.38 2.52 2.31 74.17 0.40 Maale_Efraim 1.30 103.89 1.55 1.7035.35 0.52 Mt_Arbel 1.19 121.63 2.62 2.19 58.33 0.48 Mt_Harif 1.00126.80 2.30 2.30 62.23 0.44 Neomi 1.17 99.83 1.68 1.83 38.32 0.49Neot_Kdumim 1.60 121.40 2.68 3.07 68.31 0.45 Oren_canyon 1.08 118.422.35 1.58 56.15 ND Yeruham 1.17 117.17 1.67 2.17 42.68 ND Table 7.Provided are the values of each of the parameters measured inBarleyaccessions according to the following correlation identifications(Correlation Ids): 8 = Hairiness of basal leaves; 9 = Plant height; 4 =Grain yield per spike; 11 = Stem pigmentation; 12 = Vegetative dryweight; 13 = Harvest Index.

TABLE 8 Correlation between the expression level of the selectedpolynucleotides of the invention and their homologues in specifictissues or developmental stages and the phenotypic performance acrossBarley ecotypes Gene Exp. Corr. Gene Exp. Corr. Name Set Vector R P NameSet Vector R P LYM46 B 6 0.73 0.016 LYM336 A 2 0.73 0.011 LYM302 D 110.94 0.064 LYM337 D 8 0.92 0.078 LYM304 D 6 0.93 0.073 LYM337 B 8 0.860.001 LYM304 C 6 0.74 0.009 LYM337 A 3 0.84 0.001 LYM305 D 10 0.97 0.030LYM337 A 2 0.81 0.003 LYM305 D 8 0.96 0.042 LYM337 A 8 0.78 0.004 LYM305D 9 0.94 0.059 LYM337 A 4 0.70 0.016 LYM305 C 2 0.81 0.003 LYM338 B 60.80 0.006 LYM305 C 4 0.78 0.004 LYM338 C 6 0.78 0.004 LYM305 C 3 0.780.004 LYM338 A 6 0.70 0.016 LYM305 C 8 0.75 0.008 LYM311 D 7 0.92 0.079LYM305 A 8 0.72 0.012 LYM311 C 3 0.84 0.001 LYM307 A 6 0.84 0.001 LYM311C 2 0.81 0.003 LYM308 B 2 0.72 0.019 LYM311 C 4 0.73 0.011 LYM309 B 80.71 0.020 LYM325 D 1 0.98 0.020 LYM313 D 5 1.00 0.003 LYM325 D 12 0.960.040 LYM313 D 9 0.99 0.008 LYM325 D 4 0.96 0.042 LYM313 D 10 0.98 0.016LYM325 D 5 0.95 0.045 LYM313 D 12 0.98 0.021 LYM325 D 2 0.95 0.047LYM313 D 1 0.95 0.046 LYM325 D 9 0.91 0.087 LYM313 D 8 0.93 0.067 LYM325A 2 0.91 0.000 LYM313 D 2 0.91 0.091 LYM325 A 3 0.83 0.002 LYM313 D 40.90 0.098 LYM325 A 10 0.78 0.004 LYM315 D 11 0.95 0.052 LYM325 A 4 0.710.014 LYM316 D 3 0.94 0.062 LYM346_H8 A 2 0.73 0.011 LYM317 D 11 0.950.050 LYM361_H12 A 2 0.87 0.001 LYM318 D 7 0.99 0.012 LYM361_H12 A 30.84 0.001 LYM319 D 11 0.95 0.053 LYM361_H12 A 4 0.74 0.009 LYM320 D 110.98 0.025 LYM363_H5 D 5 0.94 0.063 LYM322 D 7 0.96 0.042 LYM363_H5 D 90.92 0.082 LYM324 C 6 0.74 0.010 LYM363_H5 D 1 0.92 0.084 LYM324 C 10.71 0.015 LYM363_H5 D 12 0.90 0.100 LYM326 D 2 0.99 0.007 LYM363_H5 C 80.73 0.011 LYM326 D 1 0.99 0.010 LYM363_H5 A 8 0.73 0.011 LYM326 D 120.99 0.011 LYM376_H6 D 1 0.97 0.028 LYM326 D 4 0.99 0.011 LYM376_H6 D 50.97 0.030 LYM326 D 5 0.94 0.056 LYM376_H6 D 12 0.96 0.040 LYM326 D 30.94 0.065 LYM376_H6 D 9 0.94 0.061 LYM328 D 8 0.96 0.040 LYM376_H6 D 40.94 0.061 LYM328 D 10 0.92 0.084 LYM376_H6 D 2 0.93 0.065 LYM330 D 121.00 0.001 LYM388_H14 D 6 0.93 0.074 LYM330 D 1 0.99 0.005 LYM395_H3 D 60.91 0.093 LYM330 D 5 0.98 0.017 LYM404_H27 A 4 0.85 0.001 LYM330 D 20.98 0.024 LYM404_H27 A 5 0.80 0.003 LYM330 D 4 0.97 0.028 LYM404_H27 A12 0.75 0.008 LYM330 D 9 0.95 0.050 LYM404_H30 A 4 0.86 0.001 LYM330 D10 0.93 0.073 LYM404_H30 A 3 0.83 0.002 LYM330 A 3 0.84 0.001 LYM404_H30A 12 0.80 0.003 LYM330 A 2 0.82 0.002 LYM404_H30 A 2 0.74 0.009 LYM330 C8 0.72 0.013 LYM404_H37 A 4 0.89 0.000 LYM333 D 1 0.98 0.018 LYM404_H37A 12 0.79 0.004 LYM333 D 4 0.96 0.038 LYM404_H47 A 4 0.71 0.015 LYM333 D12 0.96 0.038 LYM404_H53 A 4 0.71 0.014 LYM333 D 2 0.96 0.043 LYM418_H15D 7 0.94 0.060 LYM333 D 5 0.95 0.046 LYM437_H8 D 11 0.99 0.009 LYM333 D9 0.91 0.089 LYM454_H4 D 3 0.95 0.048 LYM334 D 2 0.99 0.007 LYM454_H4 A6 0.77 0.005 LYM334 D 4 0.99 0.012 LYM487_H18 A 6 0.74 0.009 LYM334 D 10.98 0.018 LYM510_H1 D 5 1.00 0.002 LYM334 D 12 0.98 0.020 LYM510_H1 D12 0.99 0.011 LYM334 D 3 0.95 0.050 LYM510_H1 D 9 0.99 0.015 LYM334 D 50.92 0.076 LYM510_H1 D 10 0.97 0.028 LYM336 D 3 0.98 0.019 LYM510_H1 D 10.97 0.030 LYM336 D 2 0.92 0.083 LYM510_H1 D 2 0.93 0.068 LYM336 D 40.91 0.088 LYM510_H1 D 4 0.93 0.074 LYM336 C 2 0.86 0.001 LYM510_H1 D 80.91 0.086 LYM336 C 3 0.85 0.001 LYM510_H1 A 2 0.75 0.008 LYM336 B 20.73 0.016 LYM510_H1 C 10 0.71 0.015 Table 8. Provided are thecorrelations (R) and p-values (P) between the expression levels ofselected genes of some embodiments of the invention in various tissuesor developmental stages (Expression sets) and the phenotypic performancein various yield (seed yield, oil yield, oil content), biomass, growthrate and/or vigor components [Correlation (Corr.) vector (Vec.)Expression (Exp.)] Corr. Vector = correlation vector specified in Tables5, 6 and 7; Exp. Set = expression set specified in Table 3.

Example 4 Production of Arabidopsis Transcriptome and High ThroughputCorrelation Analysis of Yield, Biomass and/or Vigor Related ParametersUsing 44K Arabidopsis Full Genome Oligonucleotide Micro-Array

To produce a high throughput correlation analysis, the present inventorsutilized an Arabidopsis thaliana oligonucleotide micro-array, producedby Agilent Technologies [Hypertext Transfer Protocol://World Wide Web(dot) chem. (dot) agilent (dot) com/Scripts/PDS (dot) asp?1Page=50879].The array oligonucleotide represents about 40,000 A. thaliana genes andtranscripts designed based on data from the TIGR ATH1 v.5 database andArabidopsis MPSS (University of Delaware) databases. To definecorrelations between the levels of RNA expression and yield, biomasscomponents or vigor related parameters, various plant characteristics of15 different Arabidopsis ecotypes were analyzed. Among them, nineecotypes encompassing the observed variance were selected for RNAexpression analysis. The correlation between the RNA levels and thecharacterized parameters was analyzed using Pearson correlation test[Hypertext Transfer Protocol://World Wide Web (dot) davidmlane (dot)com/hyperstat/A34739 (dot) html].

Experimental Procedures

Five tissues at different developmental stages including root, leaf,flower at anthesis, seed at 5 days after flowering (DAF) and seed at 12DAF, representing different plant characteristics, were sampled and RNAwas extracted as described as described hereinabove under “GENERALEXPERIMENTAL AND BIOINFORMATICS METHODS”. For convenience, eachmicro-array expression information tissue type has received a Set ID assummarized in Table 9 below.

TABLE 9 Tissues used for Arabidopsis transcriptome expression setsExpression Set Set ID Root A Leaf B Flower C Seed 5 DAF D Seed 12 DAF ETable 9: Provided are the identification (ID) letters of each of theArabidopsis expression sets (A-E). DAF = days after flowering.

Yield Components and Vigor Related Parameters Assessment—

eight out of the nine Arabidopsis ecotypes were used in each of 5repetitive blocks (named A, B, C, D and E), each containing 20 plantsper plot. The plants were grown in a greenhouse at controlled conditionsin 22° C., and the N:P:K fertilizer (20:20:20; weight ratios) [nitrogen(N), phosphorus (P) and potassium (K)] was added. During this time datawas collected, documented and analyzed. Additional data was collectedthrough the seedling stage of plants grown in a tissue culture invertical grown transparent agar plates. Most of chosen parameters wereanalyzed by digital imaging.

Digital Imaging in Tissue Culture—

A laboratory image acquisition system was used for capturing images ofplantlets sawn in square agar plates. The image acquisition systemconsists of a digital reflex camera (Canon EOS 300D) attached to a 55 mmfocal length lens (Canon EF-S series), mounted on a reproduction device(Kaiser RS), which included 4 light units (4×150 Watts light bulb) andlocated in a darkroom.

Digital Imaging in Greenhouse—

The image capturing process was repeated every 3-4 days starting at day7 till day 30. The same camera attached to a 24 mm focal length lens(Canon EF series), placed in a custom made iron mount, was used forcapturing images of larger plants sawn in white tubs in an environmentalcontrolled greenhouse. The white tubs were square shape withmeasurements of 36×26.2 cm and 7.5 cm deep. During the capture process,the tubs were placed beneath the iron mount, while avoiding direct sunlight and casting of shadows. This process was repeated every 3-4 daysfor up to 30 days.

An image analysis system was used, which consists of a personal desktopcomputer (Intel P4 3.0 GHz processor) and a public domain program—ImageJ1.37, Java based image processing program, which was developed at theU.S National Institutes of Health and is freely available on theinternet at Hypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/.Images were captured in resolution of 6 Mega Pixels (3072×2048 pixels)and stored in a low compression JPEG (Joint Photographic Experts Groupstandard) format. Next, analyzed data was saved to text files andprocessed using the JMP statistical analysis software (SAS institute).

Leaf Analysis—

Using the digital analysis leaves data was calculated, including leafnumber, area, perimeter, length and width. On day 30, 3-4 representativeplants were chosen from each plot of blocks A, B and C. The plants weredissected, each leaf was separated and was introduced between two glasstrays, a photo of each plant was taken and the various parameters (suchas leaf total area, laminar length etc.) were calculated from theimages. The blade circularity was calculated as laminar width divided bylaminar length.

Root Analysis—

During 17 days, the different ecotypes were grown in transparent agarplates. The plates were photographed every 3 days starting at day 7 inthe photography room and the roots development was documented (seeexamples in FIGS. 3A-F). The growth rate of roots was calculatedaccording to Formula VI.

Relative growth rate of root coverage=Regression coefficient of rootcoverage along time course.  Formula VI:

Vegetative growth rate analysis—was calculated according to Formula VII.The analysis was ended with the appearance of overlapping plants.

Relative vegetative growth rate area=Regression coefficient ofvegetative area along time course.  Formula VII

For comparison between ecotypes the calculated rate was normalized usingplant developmental stage as represented by the number of true leaves.In cases where plants with 8 leaves had been sampled twice (for exampleat day 10 and day 13), only the largest sample was chosen and added tothe Anova comparison.

Seeds in Siliques Analysis—

On day 70, 15-17 siliques were collected from each plot in blocks D andE. The chosen siliques were light brown color but still intact. Thesiliques were opened in the photography room and the seeds were scatteron a glass tray, a high resolution digital picture was taken for eachplot. Using the images the number of seeds per silique was determined.

Seeds Average Weight—

At the end of the experiment all seeds from plots of blocks A-C werecollected. An average weight of 0.02 grams was measured from eachsample, the seeds were scattered on a glass tray and a picture wastaken. Using the digital analysis, the number of seeds in each samplewas calculated.

Oil Percentage in Seeds—

At the end of the experiment all seeds from plots of blocks A-C werecollected. Columbia seeds from 3 plots were mixed grounded and thenmounted onto the extraction chamber. 210 ml of n-Hexane (Cat No. 080951Biolab Ltd.) were used as the solvent. The extraction was performed for30 hours at medium heat 50° C. Once the extraction has ended then-Hexane was evaporated using the evaporator at 35° C. and vacuumconditions. The process was repeated twice. The information gained fromthe Soxhlet extractor (Soxhlet, F. Die gewichtsanalytische Bestimmungdes Milchfettes, Polytechnisches J. (Dingler's) 1879, 232, 461) was usedto create a calibration curve for the Low Resonance NMR. The content ofoil of all seed samples was determined using the Low Resonance NMR(MARAN Ultra—Oxford Instrument) and its MultiQuant sowftware package.

Silique Length Analysis—

On day 50 from sowing, 30 siliques from different plants in each plotwere sampled in block A. The chosen siliques were green-yellow in colorand were collected from the bottom parts of a grown plant's stem. Adigital photograph was taken to determine silique's length.

Dry Weight and Seed Yield—

On day 80 from sowing, the plants from blocks A-C were harvested andleft to dry at 30° C. in a drying chamber. The biomass and seed weightof each plot was separated, measured and divided by the number ofplants. Dry weight=total weight of the vegetative portion above ground(excluding roots) after drying at 30° C. in a drying chamber; Seed yieldper plant=total seed weight per plant (gr).

Oil Yield—

The oil yield was calculated using Formula VIII.

Seed Oil yield=Seed yield per plant (gr)*Oil % in seed.  Formula VIII:

Harvest Index (Seed)—

The harvest index was calculated using Formula IV (described above):Harvest Index=Average seed yield per plant/Average dry weight.

Experimental Results

Nine different Arabidopsis ecotypes were grown and characterized for 18parameters (named as vectors).

TABLE 10 Arabidopsis correlated parameters (vectors) Corre- Correlatedparameter with lation ID Root length day 13 (cm) 1 Root length day 7(cm) 2 Relative root growth (cm/day) day 13 3 Fresh weight per plant(gr) at bolting stage 4 Dry matter per plant (gr) 5 Vegetative growthrate (cm²/day) till 8 true leaves 6 Blade circularity 7 Lamina width(cm) 8 Lamina length (cm) 9 Total leaf area per plant (cm) 10 1000 Seedweight (gr) 11 Oil % per seed 12 Seeds per silique 13 Silique length(cm) 14 Seed yield per plant (gr) 15 Oil yield per plant (mg) 16 HarvestIndex 17 Leaf width/length 18 Table 10. Provided are the Arabidopsiscorrelated parameters (correlation ID Nos. 1-18). Abbreviations: Cm =centimeter(s); gr = gram(s); mg = milligram(s).

The characterized values are summarized in Tables 11 and 12 below.

TABLE 11 Measured parameters in Arabidopsis ecotypes Ecotype 15 16 12 115 17 10 13 14 An-1 0.34 118.63 34.42 0.0203 0.64 0.53 46.86 45.44 1.06Col-0 0.44 138.73 31.19 0.0230 1.27 0.35 109.89 53.47 1.26 Ct-1 0.59224.06 38.05 0.0252 1.05 0.56 58.36 58.47 1.31 Cvi 0.42 116.26 27.760.0344 1.28 0.33 56.80 35.27 1.47 (N8580) Gr-6 0.61 218.27 35.49 0.02021.69 0.37 114.66 48.56 1.24 Kondara 0.43 142.11 32.91 0.0263 1.34 0.32110.82 37.00 1.09 Ler-1 0.36 114.15 31.56 0.0205 0.81 0.45 88.49 39.381.18 Mt-0 0.62 190.06 30.79 0.0226 1.21 0.51 121.79 40.53 1.18 Shakdara0.55 187.62 34.02 0.0235 1.35 0.41 93.04 25.53 1.00 Table 11. Providedare the values of each of the parameters measured in Arabidopsisecotypes: 15 = Seed yield per plant (gram); 16 = oil yield per plant(mg); 12 = oil % per seed; 11 = 1000 seed weight (gr); 5 = dry matterper plant (gr); 17 = harvest index; 10 = total leaf area per plant (cm);13 = seeds per silique; 14 = Silique length (cm).

TABLE 12 Additional measured parameters in Arabidopsis ecotypes Ecotype6 3 2 1 4 9 8 18 7 An-1 0.313 0.631 0.937 4.419 1.510 2.767 1.385 0.3530.509 Col-0 0.378 0.664 1.759 8.530 3.607 3.544 1.697 0.288 0.481 Ct-10.484 1.176 0.701 5.621 1.935 3.274 1.460 0.316 0.450 Cvi 0.474 1.0890.728 4.834 2.082 3.785 1.374 0.258 0.370 (N8580) Gr-6 0.425 0.907 0.9915.957 3.556 3.690 1.828 0.356 0.501 Kondara 0.645 0.774 1.163 6.3724.338 4.597 1.650 0.273 0.376 Ler-1 0.430 0.606 1.284 5.649 3.467 3.8771.510 0.305 0.394 Mt-0 0.384 0.701 1.414 7.060 3.479 3.717 1.817 0.3350.491 Shakdara 0.471 0.782 1.251 7.041 3.710 4.149 1.668 0.307 0.409Table 12. Provided are the values of each of the parameters measured inArabidopsis ecotypes: 6 = Vegetative growth rate (cm²/day) until 8 trueleaves; 3 = relative root growth (cm/day) (day 13); 2 = Root length day7 (cm); 1 = Root length day 13 (cm); 4 = fresh weight per plant (gr) atbolting stage; 9. = Lamima length (cm); 8 = Lamina width (cm); 18 = Leafwidth/length; 7 = Blade circularity.

Table 13, below, provides genes of some embodiments of the invention,the characterized parameters (which are used as x axis for correlation)and the correlated tissue transcriptome along with the correlation value(R, calculated using Pearson correlation). When the correlationcoefficient (R) between the levels of a gene's expression in a certaintissue and a phenotypic performance across ecotypes is high in absolutevalue (between 0.5-1), there is an association between the gene(specifically the expression level of this gene) and the phenotypiccharacter.

TABLE 13 Correlation between the expression level of selected genes inspecific tissues or developmental stages and the phenotypic performanceacross Arabidopsis ecotypes Expres- Corre- Gene sion lation Name SetVector R P LYM297 C 1 0.77 0.024 LYM297 C 2 0.77 0.025 LYM297 D 2 0.740.056 LYM298 C 9 0.73 0.038 LYM307_H13 D 17 0.84 0.017 LYM307_H13 B 160.84 0.009 LYM307_H13 B 15 0.83 0.012 LYM307_H13 B 8 0.80 0.017LYM316_H35 D 17 0.76 0.047 LYM316_H35 C 8 0.75 0.031 LYM316_H44 B 130.70 0.051 LYM321_H23 D 2 0.90 0.006 LYM321_H23 D 1 0.83 0.021LYM321_H23 C 9 0.71 0.048 LYM361_H99 B 6 0.86 0.006 LYM361_H99 D 6 0.850.014 LYM361_H99 D 9 0.84 0.018 LYM361_H99 D 4 0.74 0.058 LYM418_H99 D 30.90 0.006 LYM418_H99 C 3 0.77 0.026 LYM418_H99 D 12 0.75 0.051LYM418_H99 B 3 0.74 0.035 LYM418_H99 E 12 0.70 0.053 Table 13. Providedare the correlations between the expression level of yield improvinggenes and their homologues in specific tissues or developmental stages(expression sets) and the phenotypic performance (correlation vector)across Arabidopsis ecotypes. The phenotypic characters [correlation(Corr.) vector (Vec.)] include yield (seed yield, oil yield, oilcontent), biomass, growth rate and/or vigor components as described inTables 10-12. Exp. Set = expression set according to Table 9hereinabove.

Example 5 Production of Arabidopsis Transcriptome and High ThroughputCorrelation Analysis of Normal and Nitrogen Limiting Conditions Using44K Arabidopsis Oligonucleotide Micro-Array

In order to produce a high throughput correlation analysis, the presentinventors utilized a Arabidopsis oligonucleotide micro-array, producedby Agilent Technologies [Hypertext Transfer Protocol://World Wide Web(dot) chem (dot) agilent (dot) com/Scripts/PDS (dot) asp?1Page=50879].The array oligonucleotide represents about 44,000 Arabidopsis genes andtranscripts. To define correlations between the levels of RNA expressionwith NUE, yield components or vigor related parameters various plantcharacteristics of 14 different Arabidopsis ecotypes were analyzed.Among them, ten ecotypes encompassing the observed variance wereselected for RNA expression analysis. The correlation between the RNAlevels and the characterized parameters was analyzed using Pearsoncorrelation test [Hypertext Transfer Protocol://World Wide Web (dot)davidmlane (dot) com/hyperstat/A34739 (dot) html].

Experimental Procedures

Two tissues of plants [leaves and stems] growing at two differentnitrogen fertilization levels (1.5 mM Nitrogen or 6 mM Nitrogen) weresampled and RNA was extracted as described hereinabove under “GENERALEXPERIMENTAL AND BIOINFORMATICS METHODS”. For convenience, eachmicro-array expression information tissue type has received a Set ID assummarized in Tablel4 below.

TABLE 14 Tissues used for Arabidopsis transcriptome expression setsExpression Set Set ID Leaves at 1.5 mM Nitrogen fertilization A Leavesat 6 mM Nitrogen fertilization B Stems at 1.5 mM Nitrogen fertilizationC Stem at 6 mM Nitrogen fertilization D Table 14: Provided are theidentification (ID) letters of each of the Arabidopsis expression sets.

Assessment of Arabidopsis Yield Components and Vigor Related ParametersUnder Different Nitrogen Fertilization Levels—

10 Arabidopsis accessions in 2 repetitive plots each containing 8 plantsper plot were grown at greenhouse. The growing protocol used was asfollows: surface sterilized seeds were sown in Eppendorf Tubes®(Eppendorf Group) containing 0.5× Murashige-Skoog basal salt medium andgrown at 23° C. under 12-hour light and 12-hour dark daily cycles for 10days. Then, seedlings of similar size were carefully transferred to potsfilled with a mix of perlite and peat in a 1:1 ratio. Constant nitrogenlimiting conditions were achieved by irrigating the plants with asolution containing 1.5 mM inorganic nitrogen in the form of KNO₃,supplemented with 2 mM CaCl₂, 1.25 mM KH₂PO₄, 1.50 mM MgSO₄, 5 mM KCl,0.01 mM H₃BO₃ and microelements, while normal irrigation conditions(Normal Nitrogen conditions) was achieved by applying a solution of 6 mMinorganic nitrogen also in the form of KNO₃, supplemented with 2 mMCaCl₂, 1.25 mM KH₂PO₄, 1.50 mM MgSO₄, 0.01 mM H₃BO₃ and microelements.To follow plant growth, trays were photographed the day nitrogenlimiting conditions were initiated and subsequently every 3 days forabout 15 additional days. Rosette plant area was then determined fromthe digital pictures. ImageJ software was used for quantifying the plantsize from the digital pictures [Hypertext Transfer Protocol://rsb (dot)info (dot) nih (dot) gov/ij] utilizing proprietary scripts designed toanalyze the size of rosette area from individual plants as a function oftime. The image analysis system included a personal desktop computer(Intel P4 3.0 GHz processor) and a public domain program—ImageJ 1.37(Java based image processing program, which was developed at the U.S.National Institutes of Health and freely available on the internet[Hypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/]. Next,analyzed data was saved to text files and processed using the JMPstatistical analysis software (SAS institute).

Data parameters collected are summarized in Table 15, hereinbelow.

TABLE 15 Arabidopsis correlated parameters (vectors) Corre- Correlatedparameter with lation Id N 1.5 mM; Rosette Area at day 8 [cm²] 1 N 1.5mM; Rosette Area at day 10 [cm²] 2 N 1.5 mM; Plot Coverage at day 8 [%]3 N 1.5 mM; Plot Coverage at day 10 [%] 4 N 1.5 mM; Leaf Number at day10 5 N 1.5 mM; Leaf Blade Area at day 10 [cm²] 6 N 1.5 mM; RGR ofRosette Area at day 3 [cm²/day] 7 N 1.5 mM; t50 Flowering [day] 8 N 1.5mM; Dry Weight [gr/plant] 9 N 1.5 mM; Seed Yield [gr/plant] 10 N 1.5 mM;Harvest Index 11 N 1.5 mM; 1000 Seeds weight [gr] 12 N 1.5 mM; seedyield/rosette area at day 10 [gr/cm²] 13 N 1.5 mM; seed yield/leaf blade[gr/cm²] 14 N 1.5 mM; % Seed yield reduction compared to N 6 mM 15 N 1.5mM; % Biomass reduction compared to N 6 mM 16 N 1.5 mM; N level/DW[SPAD ® (KONICA 17 MINOLTA SENSING, INC.) unit/gr] N 1.5 mM; DW/N level[gr/SPAD ® unit] 18 N 1.5 mM; seed yield/N level [gr/SPAD ® unit] 19 N 6mM; Rosette Area at day 8 [cm²] 20 N 6 mM; Rosette Area at day 10 [cm²]21 N 6 mM; Plot Coverage at day 8 [%] 22 N 6 mM; Plot Coverage at day 10[%] 23 N 6 mM; Leaf Number at day 10 24 N 6 mM; Leaf Blade Area at day10 25 N 6 mM; RGR of Rosette Area at day 3 [cm²/gr] 26 N 6 mM; t50Flowering [day] 27 N 6 mM; Dry Weight [gr/plant] 28 N 6 mM; Seed Yield[gr/plant] 29 N 6 mM; Harvest Index 30 N 6 mM; 1000 Seeds weight [gr] 31N 6 mM; seed yield/rosette area day at day 10 [gr/cm²] 32 N 6 mM; seedyield/leaf blade [gr/cm²] 33 N 6 mM; N level/FW 34 N 6 mM; DW/N level[gr/SPAD ® unit] 35 N 6 mM; N level/DW (SPAD ® unit/gr plant) 36 N 6 mM;Seed yield/N unit [gr/SPAD ® unit] 37 Table 15. Provided are theArabidopsis correlated parameters (vectors). “N” = Nitrogen at the notedconcentrations; “gr.” = grams; “SPAD ®” = chlorophyll levels; “t50” =time where 50% of plants flowered; “gr/SPAD ® unit” = plant biomassexpressed in grams per unit of nitrogen in plant measured by SPAD ®.“DW” = Plant Dry Weight; “FW” = Plant Fresh weight; “N level/DW” = plantNitrogen level measured in SPAD ® unit per plant biomass [gr]; “DW/Nlevel” = plant biomass per plant [gr]/SPAD ® unit; Rosette Area(measured using digital analysis); Plot Coverage at the indicated day[%] (calculated by the dividing the total plant area with the total plotarea); Leaf Blade Area at the indicated day [cm²] (measured usingdigital analysis); RGR (relative growth rate) of Rosette Area at theindicated day [cm²/day]; t50 Flowering [day] (the day in which 50% ofplant flower); seed yield/rosette area at day 10 [gr/cm²] (calculated);seed yield/leaf blade [gr/cm²] (calculated); seed yield/N level[gr/SPAD ® unit] (calculated).

Assessment of NUE, Yield Components and Vigor-Related Parameters—

Ten Arabidopsis ecotypes were grown in trays, each containing 8 plantsper plot, in a greenhouse with controlled temperature conditions forabout 12 weeks. Plants were irrigated with different nitrogenconcentration as described above depending on the treatment applied.During this time, data was collected documented and analyzed. Most ofchosen parameters were analyzed by digital imaging.

Digital Imaging—Greenhouse Assay

An image acquisition system, which consists of a digital reflex camera(Canon EOS 400D) attached with a 55 mm focal length lens (Canon EF-Sseries) placed in a custom made Aluminum mount, was used for capturingimages of plants planted in containers within an environmentalcontrolled greenhouse. The image capturing process is repeated every 2-3days starting at day 9-12 till day 16-19 (respectively) fromtransplanting.

The image processing system which was used is described in Example 4above. Images were captured in resolution of 10 Mega Pixels (3888×2592pixels) and stored in a low compression JPEG (Joint Photographic ExpertsGroup standard) format. Next, image processing output data was saved totext files and analyzed using the JMP statistical analysis software (SASinstitute).

Leaf Analysis—

Using the digital analysis leaves data was calculated, including leafnumber, leaf blade area, plot coverage, Rosette diameter and Rosettearea.

Relative Growth Rate Area:

The relative growth rate area of the rosette and the leaves wascalculated according to Formulas XII and XIV, respectively.

Seed Yield and 1000 Seeds Weight—

At the end of the experiment all seeds from all plots were collected andweighed in order to measure seed yield per plant in terms of total seedweight per plant (gr). For the calculation of 1000 seed weight, anaverage weight of 0.02 grams was measured from each sample, the seedswere scattered on a glass tray and a picture was taken. Using thedigital analysis, the number of seeds in each sample was calculated.

Dry Weight and Seed Yield—

At the end of the experiment, plant were harvested and left to dry at30° C. in a drying chamber. The biomass was separated from the seeds,weighed and divided by the number of plants. Dry weight=total weight ofthe vegetative portion above ground (excluding roots) after drying at30° C. in a drying chamber.

Harvest Index (Seed)—

The harvest index was calculated using Formula IV as described above[Harvest Index=Average seed yield per plant/Average dry weight].

T₅₀ Days to Flowering—

Each of the repeats was monitored for flowering date. Days of floweringwas calculated from sowing date till 50% of the plots flowered.

Plant Nitrogen Level—

The chlorophyll content of leaves is a good indicator of the nitrogenplant status since the degree of leaf greenness is highly correlated tothis parameter. Chlorophyll content was determined using a Minolta SPAD®502 chlorophyll meter and measurement was performed at time offlowering. SPAD® meter readings were done on young fully developed leaf.Three measurements per leaf were taken per plot. Based on thismeasurement, parameters such as the ratio between seed yield pernitrogen unit [seed yield/N level=seed yield per plant [gr]/SPAD® unit],plant DW per nitrogen unit [DW/N level=plant biomass per plant [g]/SPAD®unit], and nitrogen level per gram of biomass [N level/DW=SPAD®unit/plant biomass per plant (gr)] were calculated.

Percent of Seed Yield Reduction—

measures the amount of seeds obtained in plants when grown undernitrogen-limiting conditions compared to seed yield produced at normalnitrogen levels expressed in %.

Experimental Results

10 different Arabidopsis accessions (ecotypes) were grown andcharacterized for 37 parameters as described above. The average for eachof the measured parameters was calculated using the JMP software.Subsequent correlation analysis between the various transcriptome sets(Table 14) was conducted. Following are the results integrated to thedatabase.

TABLE 16 Correlation between the expression level of selected genes ofthe invention and their homologs in tissues under limiting or normalnitrogen fertilization and the phenotypic performance across Arabidopsisecotypes Expres- Corre- Gene sion lation Name Probe Name Set Vector R PLYM298 A_84_P21158 B 3 0.87 0.001 LYM298 A_84_P21158 B 32 0.87 0.001LYM298 A_84_P21158 A 18 0.82 0.087 LYM298 A_84_P21158 B 6 0.79 0.006LYM299 A_84_P816172 D 18 0.93 0.072 LYM299 A_84_P816172 A 18 0.91 0.031LYM299 A_84_P816172 C 18 0.91 0.032 LYM299 A_84_P127351 B 18 0.88 0.049LYM299 A_84_P127351 A 18 0.85 0.070 LYM299 A_84_P127351 A 12 0.78 0.008LYM299 A_84_P816172 B 12 0.77 0.009 LYM299 A_84_P127351 B 12 0.77 0.009LYM307_H13 A_84_P134635 C 18 0.92 0.027 LYM307_H13 A_84_P827334 C 180.86 0.061 LYM307_H13 A_84_P827334 B 15 0.77 0.009 LYM316_H35A_84_P19774 A 35 0.86 0.060 LYM316_H44 A_84_P17479 D 15 0.78 0.012LYM321_H23 A_84_P21615 C 8 0.70 0.024 LYM321_H24 A_84_P853745 C 35 0.950.015 LYM321_H24 A_84_P19280 B 15 0.75 0.012 LYM321_H24 A_84_P19280 C 80.73 0.017 LYM321_H24 A_84_P853745 C 9 0.70 0.024 LYM361_H99 A_84_P16660B 18 0.90 0.036 LYM418_H99 A_84_P591526 C 18 0.81 0.097 LYM418_H138A_84_P21797 D 35 0.96 0.042 LYM418_H138 A_84_P21797 A 17 0.91 0.031Table 16. Provided are the correlations (R) between the expressionlevels of yield improving genes and their homologs in tissues (leaves orstems) under limiting (1.5 mM Nitrogen) or normal (6 mM Nitrogen)conditions (Expression sets) and the phenotypic performance in variousyield (seed yield, oil yield, oil content), biomass, growth rate and/orvigor components [Correlation (Corr.) vector (Vec.)] under limiting ornormal Nitrogen conditions. Corr. Vec. = correlation vector according toTable 15 hereinabove; Exp. Set = expression set according to Table 14hereinabove. P = p value.

Example 6 Production of Sorghum Transcriptome and High ThroughputCorrelation Analysis with ABST Related Parameters Using 44K SorghumOligonucleotide Micro-Arrays

In order to produce a high throughput correlation analysis, the presentinventors utilized a Sorghum oligonucleotide micro-array, produced byAgilent Technologies [Hypertext Transfer Protocol://World Wide Web (dot)chem. (dot) agilent (dot) com/Scripts/PDS (dot) asp?1Page=50879]. Thearray oligonucleotide represents about 44,000 Sorghum genes andtranscripts. In order to define correlations between the levels of RNAexpression with ABST and yield components or vigor related parameters,various plant characteristics of 17 different sorghum varieties wereanalyzed. Among them, 10 varieties encompassing the observed variancewere selected for RNA expression analysis. The correlation between theRNA levels and the characterized parameters was analyzed using Pearsoncorrelation test [Hypertext Transfer Protocol://World Wide Web (dot)davidmlane (dot) com/hyperstat/A34739 (dot) html].

Correlation of Sorghum Varieties Across Ecotype Grown Under SevereDrought Conditions

Experimental Procedures

17 Sorghum varieties were grown in 3 repetitive plots in the field.Briefly, the growing protocol was as follows: sorghum seeds were sown insoil and grown under normal condition until around 35 days from sowing,around V8 (Last leaf visible, but still rolled up, ear beginning toswell). At this point, irrigation was stopped, and severe drought stresswas developed. In order to define correlations between the levels of RNAexpression with drought, yield components or vigor related parameters,the 17 different sorghum varieties were analyzed. Among them, 10varieties encompassing the observed variance were selected for RNAexpression analysis. The correlation between the RNA levels and thecharacterized parameters was analyzed using Pearson correlation test[Hypertext Transfer Protocol://World Wide Web (dot) davidmlane (dot)com/hyperstat/A34739 (dot) html].

All 10 selected Sorghum varieties were sample per each treatment. Planttissues [Flag leaf, Flower meristem and Flower] growing under severedrought stress and plants grown under Normal conditions were sampled andRNA was extracted as described hereinabove under “GENERAL EXPERIMENTALAND BIOINFORMATICS METHODS”. For convenience, each micro-arrayexpression information tissue type has received a Set ID as summarizedin Table 17 below.

TABLE 17 Sorghum transcriptome expression sets Expression Set Set IDSorghum field/Normal/flower meristem 1 Sorghum field/Normal/flower 2Sorghum field/Normal/flag leaf 3 Drought Stress: Flag leaf 4 Table 17:Provided are the sorghum transcriptome expression sets 1, 2, 3 and 4.Flag leaf = the leaf below the flower; Flower meristem = Apical meristemfollowing panicle initiation; Flower = the flower at the anthesis day.Expression sets 1, 2 and 3 are from plants grown under normalconditions. Expression set 4 derived from plants grown under droughtconditions.

The following parameters were collected using digital imaging system:

At the end of the growing period the grains were separated from thePlant ‘Head’ and the following parameters were measured and collected:

(i) Average Grain Area (cm²)—A sample of ˜200 grains were weight,photographed and images were processed using the below described imageprocessing system. The grain area was measured from those images and wasdivided by the number of grains.(ii) Average Grain Length (cm)—A sample of ˜200 grains were weight,photographed and images were processed using the below described imageprocessing system. The sum of grain lengths (longest axis) was measuredfrom those images and was divided by the number of grains.

At the end of the growing period 5 ‘Heads’ were, photographed and imageswere processed using the below described image processing system.

(i) Head Average Area (cm²) The ‘Head’ area was measured from thoseimages and was divided by the number of ‘Heads’.(ii) Head Average Length (cm) The ‘Head’ length (longest axis) wasmeasured from those images and was divided by the number of ‘Heads’.

The image processing system was used, which consists of a personaldesktop computer (Intel P4 3.0 GHz processor) and a public domainprogram—ImageJ 1.37, Java based image processing software, which wasdeveloped at the U.S. National Institutes of Health and is freelyavailable on the internet at Hypertext Transfer Protocol://rsbweb (dot)nih (dot) gov/. Images were captured in resolution of 10 Mega Pixels(3888×2592 pixels) and stored in a low compression JPEG (JointPhotographic Experts Group standard) format. Next, image processingoutput data for seed area and seed length was saved to text files andanalyzed using the JMP statistical analysis software (SAS institute).

Additional parameters were collected either by sampling 5 plants perplot or by measuring the parameter across all the plants within theplot.

Total Seed Weight/Head (Gr.)—

At the end of the experiment (plant ‘Heads’) heads from plots withinblocks A-C were collected. 5 heads were separately threshed and grainswere weighted, all additional heads were threshed together and weightedas well. The average grain weight per head was calculated by dividingthe total grain weight by number of total heads per plot (based onplot). In case of 5 heads, the total grains weight of 5 heads wasdivided by 5.

FW Head/Plant Gr—

At the end of the experiment (when heads were harvested) total and 5selected heads per plots within blocks A-C were collected separately.The heads (total and 5) were weighted (gr.) separately and the averagefresh weight per plant was calculated for total (FW Head/Plant gr basedon plot) and for 5 (FW Head/Plant gr based on 5 plants).

Plant Height—

Plants were characterized for height during growing period at 5 timepoints. In each measure, plants were measured for their height using ameasuring tape. Height was measured from ground level to top of thelongest leaf.

Plant Leaf Number—

Plants were characterized for leaf number during growing period at 5time points. In each measure, plants were measured for their leaf numberby counting all the leaves of 3 selected plants per plot.

Relative Growth Rate was calculated using Formulas IX and X.

Relative growth rate of plant height=Regression coefficient of plantheight along time course.  Formula IX

Relative growth rate of plant leaf number=Regression coefficient ofplant leaf number along time course.  Formula X

SPAD®—

Chlorophyll content was determined using a Minolta SPAD® 502 chlorophyllmeter and measurement was performed 64 days post sowing. SPAD® meterreadings were done on young fully developed leaf. Three measurements perleaf were taken per plot.

Vegetative Dry Weight and Heads—

At the end of the experiment (when Inflorescence were dry) allInflorescence and vegetative material from plots within blocks A-C werecollected. The biomass and Heads weight of each plot was separated,measured and divided by the number of Heads.

Dry weight=total weight of the vegetative portion above ground(excluding roots) after drying at 70° C. in oven for 48 hours;

Harvest Index (HI) (Sorghum)—

The harvest index was calculated using Formula XI.

Harvest Index=Average grain dry weight per Head/(Average vegetative dryweight per Head+Average Head dry weight).  Formula XI:

FW Heads/(FW Heads+FW Plants)—

The total fresh weight of heads and their respective plant biomass weremeasured at the harvest day. The heads weight was divided by the sum ofweights of heads and plants.

Experimental Results

16 different sorghum varieties were grown and characterized fordifferent parameters: The average for each of the measured parameter wascalculated using the JMP software (Tables 19-20) and a subsequentcorrelation analysis between the various transcriptome sets (Table 17)and the average parameters, was conducted (Tables 21). Results were thenintegrated to the database.

TABLE 18 Sorghum correlated parameters (vectors) Corre- CorrelationVector lation Id Average Seed Area cm²-normal A Average Seed Lengthcm-normal B FW/Plant gr based on plot-normal C FW Head/Plant gr based on5 plants-normal D FW Head/Plant gr based on plot-normal E FW Heads/(FWHeads + FW Plants) based on plot-normal F Head Average Area cm²-normal GHead Average Length cm-normal H HI-normal J Leaf SPAD ® 64 Days PostSowing-normal K Relative Growth Rate of Leaf Num-normal L RelativeGrowth Rate of Plant Height-normal M Total Seed Weight/Head gr based onplot-normal N Total Seed Weight/Head gr based on 5 heads-normal O Table18. Provided are the Sorghum correlated parameters (vectors). “gr.” =grams; “SPAD ®” = chlorophyll levels; “FW” = Plant Fresh weight;“normal” = standard growth conditions.

TABLE 19 Measured parameters in Sorghum accessions Seed Id A B C D E F GH J 20 0.1047 0.3856 162.6 406.5 175.2 0.51 120.1 25.58 200.7 21 0.11240.4017 212.6 518 223.5 0.5101 167.6 26.84 127 22 0.1313 0.4446 334.8 14856.4 0.1154 85.14 21.02 51.8 24 0.1293 0.4496 313.5 423 111.6 0.2626157.3 26.84 122.4 25 0.1204 54.53 26 0.177 93.92 27 0.1098 0.3999 151.1423.5 126.2 0.4591 168.5 31.33 327.3 28 0.1134 0.4054 137.6 386.5 107.70.4316 109.3 23.18 231.5 29 0.1022 0.3837 168 409.5 123.9 0.4249 135.125.7 241.4 30 0.118 0.4186 129 328.9 102.8 0.4416 169 28.82 304.1 310.1205 0.4302 97.62 391 82.33 0.4581 156.1 28.13 335.6 32 0.1106 0.400399.32 435.8 77.59 0.4473 112.1 22.97 349.6 33 0.1165 0.4094 112.2 429.591.17 0.4474 154.7 28.09 293.2 34 0.108 0.4008 157.4 441 150.4 0.5134171.7 30 410.9 35 0.1048 0.3947 130.5 415.8 109.1 0.4595 168.5 30.54285.1 36 0.1097 0.3953 135.7 429.5 107.6 0.4425 162.5 27.17 282.7 370.1053 0.3924 209.2 428.5 130.9 0.3856 170.5 29.26 204 Table 19:Provided are the values of each of the parameters (as described above)measured in Sorghum accessions (Seed ID) under normal and droughtconditions. Growth conditions are specified in the experimentalprocedure section.

TABLE 20 Additional measured parameters in Sorghum accessions Seed Id LM N O 20 0.1032 1.891 31.12 47.4 21 1.622 26.35 46.3 22 0.2128 3.41818.72 28.37 24 0.1862 2.425 38.38 70.4 25 0.1898 3.118 26 0.1599 3.32327 0.1957 2.178 47.67 63.45 28 0.1694 2.188 31 44.45 29 0.1821 2.57239.99 56.65 30 2.046 38.36 60 31 2.069 32.1 45.45 32 0.1754 2.547 32.6958.19 33 0.117 2.327 32.79 70.6 34 0.207 3.039 51.53 70.1 35 0.18592.335 35.71 53.95 36 0.151 2.516 38.31 59.87 37 0.24 2.81 42.44 52.65Table 20: Provided are the values of each of the parameters (asdescribed above) measured in Sorghum accessions (Seed ID) under normaland drought conditions. Growth conditions are specified in theexperimental procedure section.

TABLE 21 Correlation between the expression level of selected genes ofsome embodiments of the invention in various tissues and the phenotypicperformance under normal or abiotic stress conditions across Sorghumaccessions Gene Exp Cor R P Gene Exp Cor R P LYM419 1 N 0.73 0.025LYM491 1 N 0.71 0.031 LYM421 1 E 0.81 0.008 LYM492 1 O 0.90 0.001 LYM4211 N 0.80 0.010 LYM492 1 N 0.89 0.001 LYM421 1 O 0.77 0.016 LYM492 1 H0.85 0.003 LYM424 1 D 0.88 0.002 LYM492 1 G 0.83 0.005 LYM424 3 E 0.750.021 LYM492 1 D 0.74 0.023 LYM424 1 F 0.74 0.014 LYM493 2 D 0.84 0.005LYM424 2 E 0.71 0.032 LYM494 1 N 0.85 0.003 LYM427 1 E 0.89 0.001 LYM4941 J 0.76 0.010 LYM427 2 O 0.75 0.020 LYM494 1 G 0.76 0.018 LYM428 1 N0.81 0.008 LYM494 1 H 0.72 0.028 LYM428 1 O 0.76 0.017 LYM495 1 E 0.910.001 LYM428 1 G 0.76 0.018 LYM495 2 A 0.85 0.004 LYM428 1 H 0.73 0.027LYM495 2 B 0.83 0.005 LYM433 2 N 0.85 0.004 LYM495 1 N 0.83 0.005 LYM4352 A 0.77 0.016 LYM495 1 D 0.74 0.023 LYM435 1 N 0.75 0.020 LYM496 3 B0.91 0.001 LYM435 2 B 0.75 0.020 LYM496 3 A 0.88 0.002 LYM435 2 C 0.750.021 LYM497 1 E 0.85 0.004 LYM435 1 E 0.73 0.024 LYM497 3 A 0.85 0.004LYM436 2 C 0.90 0.001 LYM497 1 N 0.84 0.004 LYM436 2 C 0.81 0.008 LYM4973 B 0.81 0.008 LYM437 1 E 0.82 0.007 LYM497 3 C 0.80 0.010 LYM438 2 D0.89 0.001 LYM497 2 O 0.73 0.025 LYM438 2 D 0.88 0.002 LYM498 3 N 0.780.013 LYM438 2 N 0.84 0.004 LYM498 1 O 0.72 0.029 LYM438 2 O 0.79 0.012LYM498 1 J 0.72 0.020 LYM438 2 J 0.78 0.008 LYM498 1 N 0.71 0.034 LYM4382 F 0.77 0.009 LYM499 2 O 0.87 0.002 LYM438 2 E 0.74 0.022 LYM499 2 N0.80 0.010 LYM438 2 H 0.73 0.026 LYM499 2 E 0.77 0.016 LYM438 2 O 0.730.027 LYM499 1 N 0.76 0.017 LYM439 1 B 0.79 0.011 LYM499 2 J 0.73 0.016LYM439 1 A 0.74 0.023 LYM499 2 F 0.72 0.018 LYM440 2 A 0.77 0.015 LYM4992 D 0.71 0.033 LYM440 2 B 0.74 0.022 LYM500 1 N 0.78 0.012 LYM440 2 C0.71 0.031 LYM500 1 O 0.73 0.024 LYM441 1 E 0.83 0.006 LYM501 3 N 0.700.035 LYM441 2 F 0.70 0.024 LYM502 2 E 0.80 0.010 LYM442 2 A 0.75 0.021LYM503 1 C 0.87 0.002 LYM442 3 A 0.75 0.021 LYM503 3 E 0.80 0.010 LYM4423 B 0.74 0.022 LYM504 1 B 0.87 0.002 LYM442 3 C 0.71 0.032 LYM504 3 B0.86 0.003 LYM443 1 E 0.85 0.004 LYM504 1 A 0.86 0.003 LYM443 1 N 0.760.017 LYM504 3 A 0.79 0.011 LYM443 2 E 0.72 0.030 LYM505 2 N 0.81 0.009LYM444 1 B 0.83 0.006 LYM505 2 F 0.80 0.006 LYM444 1 A 0.82 0.007 LYM5052 H 0.79 0.011 LYM444 3 N 0.82 0.007 LYM505 2 D 0.77 0.014 LYM444 3 H0.73 0.026 LYM505 2 E 0.77 0.015 LYM444 3 G 0.71 0.031 LYM506 2 M 0.710.022 LYM445 2 E 0.86 0.003 LYM507 1 N 0.90 0.001 LYM445 2 H 0.85 0.003LYM507 2 O 0.80 0.009 LYM445 2 N 0.82 0.007 LYM507 2 N 0.73 0.026 LYM4452 G 0.75 0.019 LYM507 3 A 0.72 0.030 LYM445 2 D 0.74 0.022 LYM507 1 O0.71 0.032 LYM445 3 G 0.73 0.024 LYM508 1 B 0.92 0.000 LYM445 3 E 0.730.025 LYM508 1 A 0.88 0.002 LYM445 3 O 0.72 0.030 LYM508 1 C 0.88 0.002LYM446 2 B 0.86 0.003 LYM508 1 M 0.82 0.003 LYM446 2 A 0.83 0.005 LYM4202 D 0.91 0.001 LYM446 1 N 0.77 0.015 LYM420 2 N 0.86 0.003 LYM446 1 G0.75 0.021 LYM420 2 G 0.81 0.008 LYM446 1 O 0.72 0.027 LYM420 2 H 0.800.010 LYM446 1 H 0.71 0.033 LYM420 2 F 0.79 0.007 LYM447 1 N 0.70 0.035LYM420 1 A 0.77 0.014 LYM448 2 A 0.75 0.021 LYM420 3 N 0.74 0.021 LYM4482 C 0.74 0.022 LYM420 2 J 0.73 0.017 LYM448 2 B 0.73 0.025 LYM422 1 C0.95 0.000 LYM449 2 M 0.88 0.001 LYM422 2 C 0.89 0.001 LYM449 2 C 0.770.015 LYM422 2 M 0.78 0.007 LYM449 1 O 0.73 0.025 LYM422 2 A 0.75 0.019LYM450 1 N 0.77 0.016 LYM422 1 A 0.75 0.020 LYM451 2 A 0.89 0.001 LYM4221 B 0.71 0.034 LYM451 2 B 0.83 0.005 LYM422 2 B 0.70 0.035 LYM451 1 B0.83 0.005 LYM423 1 E 0.81 0.008 LYM451 3 B 0.80 0.009 LYM423 1 N 0.770.015 LYM451 1 A 0.77 0.014 LYM425 1 N 0.89 0.001 LYM451 3 A 0.73 0.027LYM425 1 H 0.86 0.003 LYM452 3 A 0.83 0.005 LYM425 1 G 0.86 0.003 LYM4523 B 0.81 0.008 LYM425 1 O 0.79 0.012 LYM452 3 C 0.77 0.015 LYM425 2 G0.75 0.021 LYM452 2 O 0.71 0.034 LYM425 3 G 0.74 0.023 LYM453 1 B 0.950.000 LYM425 2 O 0.70 0.034 LYM453 1 A 0.89 0.001 LYM426 1 N 0.78 0.014LYM455 2 E 0.72 0.029 LYM429 1 E 0.76 0.018 LYM456 1 B 0.88 0.002 LYM4301 N 0.86 0.003 LYM456 1 A 0.83 0.006 LYM430 1 O 0.71 0.033 LYM456 2 A0.80 0.010 LYM431 3 N 0.80 0.010 LYM456 3 B 0.74 0.023 LYM431 2 E 0.790.011 LYM456 2 B 0.71 0.033 LYM431 1 B 0.76 0.017 LYM456 3 A 0.71 0.033LYM431 1 A 0.72 0.028 LYM456 2 C 0.70 0.034 LYM431 3 E 0.71 0.033 LYM4572 A 0.89 0.001 LYM432 1 H 0.84 0.004 LYM457 2 B 0.86 0.003 LYM432 1 G0.74 0.022 LYM457 2 C 0.76 0.018 LYM432 1 N 0.74 0.024 LYM457 3 A 0.750.020 LYM432 1 D 0.72 0.030 LYM457 3 B 0.74 0.022 LYM434 2 A 0.89 0.001LYM458 1 A 0.87 0.003 LYM434 1 O 0.86 0.003 LYM458 1 B 0.81 0.009 LYM4342 A 0.84 0.005 LYM458 3 A 0.74 0.022 LYM434 2 B 0.81 0.009 LYM458 3 B0.74 0.023 LYM434 1 N 0.80 0.010 LYM458 2 N 0.73 0.027 LYM434 1 H 0.780.014 LYM460 1 N 0.73 0.027 LYM434 1 G 0.76 0.018 LYM461 1 E 0.74 0.023LYM434 2 B 0.74 0.023 LYM463 2 K 0.82 0.004 LYM434 1 D 0.71 0.033 LYM4651 C 0.95 0.000 LYM307_H7 1 N 0.81 0.009 LYM465 1 A 0.75 0.020 LYM307_ H71 O 0.75 0.021 LYM465 1 B 0.71 0.034 LYM315_H4 2 A 0.90 0.001 LYM466 3 N0.72 0.028 LYM315_H4 2 B 0.85 0.004 LYM467 2 O 0.75 0.019 LYM316_H39 3 B0.71 0.031 LYM467 2 H 0.73 0.027 LYM316_H39 3 A 0.71 0.034 LYM468 1 B0.89 0.001 LYM317_H8 2 E 0.86 0.003 LYM468 1 A 0.86 0.003 LYM318_H7 2 O0.79 0.012 LYM468 3 B 0.74 0.023 LYM318_H7 3 L 0.76 0.030 LYM468 3 C0.72 0.030 LYM321_H7 2 O 0.91 0.001 LYM468 3 A 0.70 0.035 LYM321_H7 2 F0.73 0.017 LYM472 2 A 0.91 0.001 LYM326_H5 2 A 0.81 0.008 LYM472 2 B0.90 0.001 LYM326_H5 2 B 0.77 0.015 LYM472 1 E 0.84 0.005 LYM326_H5 2 C0.75 0.020 LYM472 1 F 0.77 0.009 LYM332_H6 1 B 0.83 0.005 LYM472 1 O0.77 0.016 LYM332_H6 1 A 0.79 0.012 LYM472 1 D 0.76 0.016 LYM348_H1 1 O0.78 0.013 LYM472 1 N 0.74 0.022 LYM348_H1 1 N 0.77 0.015 LYM473 1 G0.85 0.004 LYM349_H1 1 N 0.80 0.010 LYM473 1 N 0.84 0.005 LYM349_H1 1 E0.71 0.032 LYM473 1 H 0.81 0.008 LYM353_H1 2 C 0.89 0.001 LYM473 1 E0.76 0.017 LYM353_H1 2 A 0.75 0.019 LYM473 3 E 0.76 0.019 LYM353_H1 2 B0.70 0.035 LYM473 1 O 0.75 0.019 LYM357_H1 2 A 0.89 0.001 LYM474 1 E0.98 0.000 LYM357_H1 2 B 0.85 0.004 LYM474 1 N 0.86 0.003 LYM360_H1 2 O0.76 0.017 LYM474 2 A 0.80 0.009 LYM363_H1 2 F 0.75 0.013 LYM474 1 O0.78 0.014 LYM364_H1 1 E 0.70 0.034 LYM474 2 B 0.77 0.016 LYM364_H1 1 N0.70 0.035 LYM474 1 D 0.75 0.020 LYM365_H1 3 B 0.74 0.023 LYM475 2 G0.79 0.012 LYM365_H1 3 A 0.70 0.034 LYM475 2 N 0.75 0.019 LYM368_H4 1 N0.84 0.005 LYM475 2 H 0.75 0.021 LYM368_H4 3 C 0.80 0.009 LYM476 2 C0.87 0.002 LYM368_H4 3 A 0.76 0.017 LYM476 2 A 0.73 0.024 LYM373_H1 3 E0.73 0.025 LYM476 2 B 0.70 0.035 LYM375_H1 1 C 0.84 0.004 LYM477 1 B0.92 0.000 LYM375_H1 2 L 0.80 0.016 LYM477 1 A 0.92 0.000 LYM376_H2 1 B0.86 0.003 LYM477 3 B 0.80 0.010 LYM376_H2 1 A 0.84 0.004 LYM477 3 A0.78 0.013 LYM382_H3 1 B 0.88 0.002 LYM477 2 C 0.75 0.019 LYM382_H3 1 A0.86 0.003 LYM478 1 A 0.85 0.004 LYM388_H2 2 C 0.70 0.035 LYM478 1 B0.82 0.007 LYM392_H2 2 E 0.72 0.028 LYM478 1 C 0.79 0.012 LYM395_H5 2 A0.90 0.001 LYM479 3 E 0.71 0.034 LYM395_H5 2 B 0.85 0.004 LYM480 1 C0.91 0.001 LYM404_H55 1 B 0.86 0.003 LYM480 3 C 0.89 0.001 LYM404_H55 1C 0.82 0.006 LYM480 1 A 0.80 0.010 LYM404_H55 1 A 0.81 0.009 LYM480 1 B0.77 0.015 LYM407_H14 1 C 0.81 0.008 LYM480 3 A 0.76 0.018 LYM407_H14 1A 0.71 0.033 LYM480 3 B 0.70 0.034 LYM407_H16 1 A 0.83 0.006 LYM480 3 M0.70 0.024 LYM407_H16 1 B 0.80 0.009 LYM481 1 B 0.90 0.001 LYM407_H16 1C 0.74 0.022 LYM481 1 A 0.86 0.003 LYM410_H2 1 N 0.74 0.023 LYM481 3 E0.83 0.005 LYM410_H2 1 E 0.73 0.026 LYM483 1 N 0.74 0.022 LYM410_H2 2 O0.71 0.032 LYM484 1 G 0.90 0.001 LYM415_H1 3 E 0.71 0.034 LYM484 1 H0.89 0.001 LYM416_H4 1 N 0.90 0.001 LYM484 3 G 0.86 0.003 LYM416_H4 1 G0.89 0.001 LYM484 1 N 0.81 0.008 LYM416_H4 1 H 0.87 0.002 LYM484 3 H0.76 0.018 LYM416_H4 1 J 0.79 0.007 LYM484 1 O 0.74 0.021 LYM416_H4 1 O0.79 0.012 LYM484 3 N 0.72 0.027 LYM418_H39 1 N 0.78 0.012 LYM484 2 G0.72 0.028 LYM418_H39 1 E 0.77 0.015 LYM485 2 F 0.73 0.017 LYM418_H146 2A 0.77 0.016 LYM486 1 N 0.84 0.004 LYM427_H1 1 E 0.92 0.000 LYM486 1 E0.83 0.006 LYM427_H1 2 O 0.75 0.019 LYM487 1 N 0.75 0.021 LYM475_H1 2 D0.82 0.007 LYM487 1 H 0.73 0.025 LYM475_H1 2 G 0.79 0.012 LYM488 1 N0.76 0.017 LYM475_H1 2 N 0.75 0.019 LYM488 2 O 0.71 0.032 LYM475_H1 2 H0.75 0.021 LYM488 1 E 0.71 0.033 LYM483_H1 1 N 0.77 0.016 LYM489 2 C0.78 0.012 LYM489_H2 1 B 0.83 0.005 LYM490 3 B 0.78 0.013 LYM489_H2 1 A0.77 0.016 LYM490 1 B 0.76 0.017 LYM497_H1 3 A 0.86 0.003 LYM490 3 A0.75 0.021 LYM497_H1 3 B 0.82 0.006 LYM490 1 A 0.70 0.035 LYM497_H1 3 C0.73 0.027 Table 21. Provided are the correlations (R) between theexpression levels of yield improving genes and their homologs in tissues[Flag leaf, Flower meristem and Flower; Expression sets (Exp)] and thephenotypic performance in various yield, biomass, growth rate and/orvigor components [Correlation vector (cor)] under stress conditions ornormal conditions across Sorghum accessions. P = p value.

Sorghum Vigor Related Parameters Under 100 mM NaCl and Low Temperature(10±2° C.)—

Ten Sorghum varieties were grown in 3 repetitive plots, each containing17 plants, at a net house under semi-hydroponics conditions. Briefly,the growing protocol was as follows: Sorghum seeds were sown in traysfilled with a mix of vermiculite and peat in a 1:1 ratio. Followinggermination, the trays were transferred to the high salinity solution(100 mM NaCl in addition to the Full Hogland solution), low temperature(10±2° C. in the presence of Full Hogland solution) or at Normal growthsolution [Full Hogland solution at 28±2° C.].

Full Hogland solution consists of: KNO₃—0.808 grams/liter, MgSO₄-0.12grams/liter, KH₂PO₄-0.172 grams/liter and 0.01% (volume/volume) of‘Super coratin’ micro elements (Iron-EDDHA[ethylenediamine-N,N′-bis(2-hydroxyphenylacetic acid)]—40.5 grams/liter;Mn—20.2 grams/liter; Zn 10.1 grams/liter; Co 1.5 grams/liter; and Mo 1.1grams/liter), solution's pH should be 6.5-6.8].

All 10 selected Sorghum varieties were sampled per each treatment. Twotissues [leaves and roots] growing at 100 mM NaCl, low temperature(10±2° C.) or under Normal conditions (full Hogland at a temperaturebetween 28±2° C.) were sampled and RNA was extracted as describedhereinabove under “GENERAL EXPERIMENTAL AND BIOINFORMATICS METHODS”.

TABLE 22 Sorghum transcriptome expression sets Expression Set Set IDSorghum roots under cold 1 Sorghum vegetative meristem NaCl 2 Sorghumvegetative meristem under low nitrogen 3 Sorghum vegetative meristemunder cold conditions 4 Sorghum roots under NaCl 5 Sorghum vegetativemeristem under normal conditions 6 Sorghum roots under low nitrogen 7Sorghum roots under normal 8 Table 22: Provided are the Sorghumtranscriptome expression sets. Cold conditions = 10 ± 2° C.; NaCl = 100mM NaCl; low nitrogen = 1.2 mM Nitrogen; Normal conditions = 16 mMNitrogen.

Experimental Results

10 different Sorghum varieties were grown and characterized for thefollowing parameters: “Leaf number Normal”=leaf number per plant undernormal conditions (average of five plants); “Plant Height Normal”=plantheight under normal conditions (average of five plants); “Root DW 100 mMNaCl”—root dry weight per plant under salinity conditions (average offive plants); The average for each of the measured parameter wascalculated using the JMP software and values are summarized in Table 24below. Subsequent correlation analysis between the various transcriptomesets and the average parameters were conducted (Table 25). Results werethen integrated to the database.

TABLE 23 Sorghum correlated parameters (vectors) Correlation VectorCorr. Id DW Root/Plant - Cold A DW Root/Plant - 100 mM NaCl B DWShoot/Plant - Low Nitrogen C DW Root/Plant - Low Nitrogen D Leaf numberTP-3* - Cold E Leaf number TP-3*- 100 mM NaCl F Plant Height TP-3*- 100mM NaCl G DW Shoot/Plant - Cold H DW Shoot/Plant - Normal I Plant HeightTP-3* - Low Nitrogen J Leaf number TP-3* - Low Nitrogen K DWShoot/Plant - 100 mM NaCl L Leaf number TP-3* - Normal M DW Root/Plant -Normal N Table 23: Provided are the Sorghum correlated parameters. Coldconditions = 10 ± 2° C.; NaCl = 100 mM NaCl; low nitrogen = 1.2 mMNitrogen; Normal conditions = 16 mM Nitrogen *TP-3 refers to time point3.

TABLE 24 Sorghum accessions, measured parameters Seed ID F B L G E A H MI 20 3.67 0.35 0.66 14.63 3.88 0.83 1.03 4.17 0.81 22 3.88 1.45 2.4316.31 4.16 0.95 1.34 4.48 1.89 26 4.28 1.49 2.40 20.56 4.52 1.47 1.714.93 2.51 27 4.03 0.81 1.61 14.70 4.28 1.06 1.28 4.53 1.26 28 3.97 1.031.77 16.43 4.33 0.71 1.12 4.52 1.55 29 3.98 0.95 1.66 16.12 4.17 1.381.69 4.64 1.50 30 3.90 2.00 2.23 15.61 3.94 2.04 2.24 4.49 1.93 31 4.181.39 2.76 18.71 4.26 1.03 1.26 4.79 1.95 34 3.70 1.29 1.29 13.65 4.201.01 1.08 4.37 1.48 37 3.82 1.76 1.55 15.72 4.04 1.01 1.02 4.54 1.85Table 24: Provided are the measured parameters under 100 mM NaCl and lowtemperature (8-10° C.) conditions of Sorghum accessions (Seed ID)according to the Correlation ID numbers (described in Table 23 above) asfollows: F [100 mM NaCl: leaf Number]; B [100 mM NaCl: Root DW]; L [100mM NaCl: Shoot DW]; G [100 mM NaCl: Plant height]; E [low temperature:leaf Number]; A [low temperature: Root DW]; H [low temperature: ShootDW]; M [Normal: leaf Number]; I [Normal: Shoot DW].

TABLE 25 Correlation between the expression level of selected genes ofsome embodiments of the invention in roots and the phenotypicperformance under normal or abiotic stress conditions across Sorghumaccessions Gene Exp Cor R P LYM304_H7 7 K 0.87 0.012 LYM304_H7 7 J 0.710.077 LYM307_H7 3 C 0.72 0.030 LYM307_H7 3 J 0.85 0.004 LYM307_H7 4 A0.83 0.006 LYM307_H7 4 H 0.88 0.002 LYM307_H7 5 F 0.85 0.068 LYM307_H7 5G 0.87 0.054 LYM307_H7 7 D 0.74 0.056 LYM307_H7 7 J 0.82 0.024 LYM307_H78 N 0.86 0.003 LYM307_H7 8 I 0.75 0.020 LYM307_H7 8 M 0.78 0.014LYM315_H4 5 F 0.98 0.002 LYM315_H4 5 G 0.87 0.056 LYM316_H10 7 K 0.930.002 LYM316_H10 7 K 0.72 0.065 LYM316_H39 7 J 0.70 0.078 LYM317_H8 3 D0.73 0.025 LYM318_H7 2 B 0.75 0.020 LYM321_H7 7 C 0.72 0.070 LYM321_H7 7J 0.73 0.061 LYM346_H2 3 D 0.75 0.020 LYM346_H2 3 C 0.72 0.028 LYM346_H24 A 0.71 0.034 LYM346_H2 4 H 0.87 0.002 LYM346_H5 4 A 0.71 0.033LYM348_H1 2 B 0.72 0.029 LYM348_H1 4 A 0.70 0.034 LYM348_H1 4 H 0.860.003 LYM349_H1 4 H 0.79 0.011 LYM350_H1 5 G 0.93 0.021 LYM350_H1 7 D0.86 0.012 LYM350_H1 7 C 0.82 0.025 LYM350_H1 7 J 0.81 0.027 LYM353_H1 5G 0.85 0.066 LYM359_H1 8 N 0.77 0.016 LYM359_H1 8 I 0.73 0.025 LYM360_H17 D 0.78 0.038 LYM360_H1 7 C 0.70 0.079 LYM361_H13 4 H 0.80 0.009LYM361_H7 5 G 0.99 0.001 LYM363_H1 5 G 0.99 0.002 LYM363_H1 7 C 0.870.012 LYM363_H1 7 J 0.84 0.017 LYM364_H1 2 B 0.76 0.018 LYM364_H1 4 H0.76 0.018 LYM365_H1 4 H 0.73 0.024 LYM365_H1 7 J 0.73 0.063 LYM368_H4 4H 0.82 0.007 LYM369_H3 7 K 0.74 0.059 LYM373_H1 5 F 0.86 0.062 LYM375_H11 E 0.71 0.021 LYM375_H1 3 J 0.75 0.020 LYM375_H1 5 L 0.88 0.048LYM375_H1 5 F 0.89 0.041 LYM375_H1 5 G 0.85 0.070 LYM375_H1 7 J 0.830.022 LYM375_H1 8 N 0.79 0.011 LYM387_H4 7 J 0.83 0.020 LYM388_H2 4 H0.75 0.020 LYM388_H2 7 K 0.77 0.045 LYM392_H2 6 N 0.75 0.020 LYM392_H2 6I 0.77 0.015 LYM393_H2 2 F 0.77 0.015 LYM400_H2 1 E 0.81 0.005 LYM400_H27 C 0.80 0.032 LYM402_H2 7 K 0.82 0.023 LYM410_H2 7 D 0.75 0.053LYM415_H1 1 A 0.70 0.024 LYM416_H4 3 D 0.89 0.001 LYM416_H4 3 C 0.890.001 LYM416_H4 3 K 0.71 0.033 LYM416_H4 3 J 0.85 0.003 LYM416_H4 4 A0.72 0.030 LYM416_H4 4 H 0.79 0.012 LYM416_H4 5 L 0.88 0.050 LYM416_H4 5F 0.83 0.085 LYM416_H4 7 D 0.73 0.060 LYM416_H4 7 C 0.88 0.010 LYM416_H47 J 0.72 0.069 LYM419 5 L 0.81 0.098 LYM428 4 H 0.87 0.002 LYM430 7 D0.71 0.074 LYM430 7 C 0.73 0.064 LYM433 2 B 0.71 0.033 LYM434 4 A 0.760.016 LYM434 4 H 0.90 0.001 LYM440 5 G 0.82 0.089 LYM441 3 D 0.81 0.007LYM441 3 C 0.75 0.020 LYM441 3 J 0.80 0.010 LYM443 2 L 0.83 0.006 LYM4463 D 0.74 0.022 LYM446 3 J 0.72 0.028 LYM446 4 A 0.84 0.005 LYM446 4 H0.78 0.014 LYM446 5 L 0.92 0.029 LYM446 7 D 0.73 0.063 LYM446 8 N 0.810.008 LYM446 8 I 0.77 0.016 LYM446 8 M 0.82 0.006 LYM447 2 B 0.71 0.033LYM447 3 D 0.75 0.020 LYM448 3 D 0.94 0.000 LYM448 3 C 0.89 0.001 LYM4483 J 0.84 0.004 LYM449 1 A 0.82 0.004 LYM450 3 K 0.75 0.021 LYM450 3 J0.76 0.018 LYM450 4 A 0.79 0.011 LYM450 4 H 0.83 0.006 LYM450 5 F 0.900.040 LYM450 5 G 0.90 0.039 LYM450 7 D 0.86 0.014 LYM450 7 C 0.81 0.028LYM450 7 K 0.71 0.071 LYM450 7 J 0.79 0.036 LYM451 2 F 0.74 0.023 LYM4512 G 0.87 0.002 LYM453 4 A 0.77 0.015 LYM453 5 B 0.95 0.015 LYM456 7 K0.72 0.068 LYM457 3 D 0.80 0.010 LYM457 3 C 0.82 0.007 LYM457 3 K 0.720.029 LYM457 4 H 0.74 0.022 LYM460 3 K 0.75 0.021 LYM460 3 J 0.74 0.024LYM460 6 N 0.76 0.018 LYM460 6 I 0.78 0.012 LYM460 7 K 0.73 0.064 LYM4637 D 0.74 0.058 LYM463 7 C 0.71 0.075 LYM463 7 J 0.77 0.043 LYM464 2 B0.76 0.017 LYM464 5 L 0.99 0.002 LYM464 8 N 0.87 0.002 LYM464 8 I 0.800.010 LYM466 5 F 0.81 0.097 LYM468 1 A 0.72 0.019 LYM468 2 B 0.71 0.033LYM468 3 D 0.76 0.017 LYM477 2 G 0.81 0.008 LYM481 2 G 0.74 0.023 LYM4816 M 0.71 0.032 LYM483 2 B 0.77 0.016 LYM484 7 D 0.76 0.050 LYM485 5 F0.98 0.003 LYM485 5 G 0.86 0.060 LYM488 3 D 0.75 0.020 LYM488 3 C 0.800.009 LYM488 4 H 0.76 0.019 LYM490 2 F 0.73 0.026 LYM490 7 D 0.74 0.057LYM490 7 C 0.71 0.075 LYM490 7 K 0.84 0.019 LYM491 3 D 0.71 0.033 LYM4913 C 0.72 0.028 LYM491 6 N 0.75 0.021 LYM491 6 I 0.79 0.012 LYM495 3 D0.72 0.028 LYM495 3 C 0.72 0.028 LYM495 4 H 0.71 0.032 LYM496 7 K 0.740.056 LYM499 2 B 0.74 0.024 LYM501 7 K 0.74 0.059 LYM505 8 N 0.82 0.007LYM505 8 I 0.72 0.029 LYM508 3 K 0.76 0.017 LYM508 5 B 0.85 0.068 LYM5085 F 0.98 0.004 LYM508 5 G 0.83 0.079 LYM508 7 K 0.76 0.046 LYM509 5 F0.91 0.034 LYM509 5 G 0.82 0.089 LYM509 7 C 0.76 0.045 LYM509 7 K 0.860.013 LYM509 7 J 0.74 0.057 Table 25. Provided are the correlations (R)between the expression levels yield improving genes and their homologsin various tissues [Expression sets (Exp)] and the phenotypicperformance [yield, biomass, growth rate and/or vigor components(Correlation vector)] under abiotic stress conditions (salinity) ornormal conditions across Sorghum accessions. Cor-Correlation vector asdescribed hereinabove (Table 23). P = p value.

Example 7 Production of Maize Transcriptome and High ThroughputCorrelation Analysis Using 60K Maize Oligonucleotide Micro-Array

To produce a high throughput correlation analysis, the present inventorsutilized a Maize oligonucleotide micro-array, produced by AgilentTechnologies [Hypertxt Transfer Protocol://World Wide Web (dot) chem.(dot) agilent (dot) com/Scripts/PDS (dot) asp?1Page=50879]. The arrayoligonucleotide represents about 46K Maize genes and transcriptsdesigned based on data from Public databases (Example 1). To definecorrelations between the levels of RNA expression and yield, biomasscomponents or vigor related parameters, various plant characteristics of12 different Maize hybrids were analyzed. Among them, 10 hybridsencompassing the observed variance were selected for RNA expressionanalysis. The correlation between the RNA levels and the characterizedparameters was analyzed using Pearson correlation test [HypertextTransfer Protocol://World Wide Web (dot) davidmlane (dot)com/hyperstat/A34739 (dot) html].

Experimental Procedures

Five tissues at different developmental stages including Ear(flowering-R1), leaf (flowering-R1), Leaf Grain from the basal ear part,Grain from the distal ear, representing different plant characteristics,were sampled and RNA was extracted as described in “GENERAL EXPERIMENTALAND BIOINFORMATICS METHODS”. For convenience, each micro-arrayexpression information tissue type has received a Set ID as summarizedin Table 26 below.

TABLE 26 Tissues used for Maize transcriptome expression sets ExpressionSet Set ID EAR Flowering-R1 A EAR R2-R3 B Grain basal R3-R5 C Graindistal R3-R5 D Internode V6 E Internode Flowering-R1 F Internode R2-R3 GLeaf V6 H Leaf Flowering-R1 J Leaf R3-R5 K Table 26: Provided are theidentification (ID) letters of each of the Maize expression sets (A-K).

The following parameters were collected:

Grain Area (cm²)—

At the end of the growing period the grains were separated from the ear.A sample of ˜200 grains were weight, photographed and images wereprocessed using the below described image processing system. The grainarea was measured from those images and was divided by the number ofgrains.

Grain Length and Grain Width (cm)—

At the end of the growing period the grains were separated from the ear.A sample of ˜200 grains were weight, photographed and images wereprocessed using the below described image processing system. The sum ofgrain lengths/or width (longest axis) was measured from those images andwas divided by the number of grains.

Ear Area (cm²)—

At the end of the growing period 6 ears were, photographed and imageswere processed using the below described image processing system. TheEar area was measured from those images and was divided by the number ofEars.

Ear Length and Ear Width (cm)

At the end of the growing period 6 ears were, photographed and imageswere processed using the below described image processing system. TheEar length and width (longest axis) was measured from those images andwas divided by the number of ears.

The image processing system was used, which consists of a personaldesktop computer (Intel P4 3.0 GHz processor) and a public domainprogram—ImageJ 1.37, Java based image processing software, which wasdeveloped at the U.S. National Institutes of Health and is freelyavailable on the internet at Hypertext Transfer Protocol://rsbweb (dot)nih (dot) gov/. Images were captured in resolution of 10 Mega Pixels(3888×2592 pixels) and stored in a low compression JPEG (JointPhotographic Experts Group standard) format. Next, image processingoutput data for seed area and seed length was saved to text files andanalyzed using the JMP statistical analysis software (SAS institute).

Additional parameters were collected either by sampling 6 plants perplot or by measuring the parameter across all the plants within theplot.

Normalized Grain Weight Per Plant (gr.)—

At the end of the experiment all ears from plots within blocks A-C werecollected. 6 ears were separately threshed and grains were weighted, alladditional ears were threshed together and weighted as well. The grainweight was normalized using the relative humidity to be 0%. Thenormalized average grain weight per ear was calculated by dividing thetotal normalized grain weight by the total number of ears per plot(based on plot). In case of 6 ears, the total grains weight of 6 earswas divided by 6.

Ear FW (gr.)—

At the end of the experiment (when ears were harvested) total and 6selected ears per plots within blocks A-C were collected separately. Theplants with (total and 6) were weighted (gr.) separately and the averageear per plant was calculated for total (Ear FW per plot) and for 6 (EarFW per plant).

Plant Height and Ear Height—

Plants were characterized for height at harvesting. In each measure, 6plants were measured for their height using a measuring tape. Height wasmeasured from ground level to top of the plant below the tassel. Earheight was measured from the ground level to the place were the main earis located

Leaf Number Per Plant—

Plants were characterized for leaf number during growing period at 5time points. In each measure, plants were measured for their leaf numberby counting all the leaves of 3 selected plants per plot.

Relative Growth Rate was calculated using regression coefficient of leafnumber change a long time course.

SPAD®—

Chlorophyll content was determined using a Minolta SPAD® 502 chlorophyllmeter and measurement was performed 64 days post sowing. SPAD® meterreadings were done on young fully developed leaf. Three measurements perleaf were taken per plot. Data were taken after 46 and 54 days aftersowing (DPS)

Dry Weight Per Plant—

At the end of the experiment when all vegetative material from plotswithin blocks A-C were collected, weight and divided by the number ofplants.

Ear Diameter [cm]—

The diameter of the ear at the mid of the ear was measured using aruler.

Cob Diameter [cm]—

The diameter of the cob without grains was measured using a ruler.

Kernel Row Number Per Ear—

The number of rows in each ear was counted. The average of 6 ears perplot was calculated.

TABLE 27 Maize correlated parameters (vectors) Corre- Correlatedparameter with lation ID Growth rate (Leaf No based) 1 Plant Height perplot (cm) 2 Ear Height (cm) 3 Leaf Number per plant 4 Ear Length (cm) 5Kernel Row Number per Ear 6 Ear Width (mm) 7 Cob diameter (mm) 8 Ear FWper plant (gr) (based on 6) 9 Normalized Grain Weight per plant (gr)(based on 6) 10 Ears FW per plant (gr) (based on all) 11 Normalizedgrain weight per plant (gr) (based on all) 12 Ear Area [cm²] 13 EarWidth [cm] 14 Grain Area [cm²] 15 Grain Length [cm] 16 Grain Width [cm]17 DW per plant (gr) (based on 6) 18 Table 27.

Twelve maize varieties were grown, and characterized for parameters, asdescribed above. The average for each parameter was calculated using theJMP software, and values are summarized in Tables 28 below. Subsequentcorrelation between the various transcriptome sets for all or sub set oflines was done by the bioinformatic unit and results were integratedinto the database.

TABLE 28 Measured parameters in Maize Hybrid Growth Plant rate HeightLeaf Kernel (Leaf per Ear Number Ear Row Geno- No plot Height per LengthNumber # type based) (cm) (cm) plant (cm) per Ear 1 Line 1 0.306 286.944135.278 11.944 19.500 16.167 2 Line 2 0.283 278.083 135.167 12.00018.917 16.167 3 Line 3 0.221 260.5 122.333 11.11 20.167 14.667 4 Line 40.281 275.133 131.967 11.689 19.456 16.200 5 Line 5 0.269 238.500114.000 11.778 19.889 15.889 6 Line 6 0.244 224.833 94.278 12.333 17.72215.167 7 Line 7 0.244 264.444 120.944 12.444 17.667 16.000 8 Line 80.266 251.611 107.722 12.222 17.278 14.833 9 Line 9 0.304 178.000 62.167 9.667 16.667 16 10 Line 10 0.308 279.000 139.667 11.667 17.500 17.66711 Line 11 0.301 278.444 112.500 12.556 20.500 15.389 12 Line 12 0.194163.778 60.444  9.278 19.856 14.267 Table 28.

TABLE 29 Measured parameters in Maize Hybrid additional parametersNormal- Normal- ized Ears ized Ear FW Grain FW grain per Weight perweight plant per plant per Ear Cob (gr) plant (gr) plant (gr) Geno-Width diameter (based (gr) (based (based (based on # type (mm) (mm) on6) on 6) on all) all) 1 Line 1 51.407 28.715 272.222 156.614 280.106140.463 2 Line 2 50.136 28.957 245.833 140.683 278.194 153.900 3 Line 346.29 25.078 208.333 139.536 217.502 135.882 4 Line 4 49.923 28.052262.222 153.667 288.280 152.500 5 Line 5 47.632 25.732 263.889 176.983247.879 159.156 6 Line 6 47.420 25.783 177.778 119.667 175.841 117.135 7Line 7 47.253 26.432 188.889 119.692 192.474 123.237 8 Line 8 46.84625.192 197.222 133.508 204.700 131.266 9 Line 9 41.822 24.342 108.33372.875 10 Line 10 48.283 26.933 175.000 113.850 257.692 153.260 11 Line11 49.275 26.668 261.111 173.231 264.236 170.662 12 Line 12 41.837141.111 54.316 142.716 40.844 Table 29.

TABLE 30 Measured parameters in Maize Hybrid additional parameters DWper Ear Ear Grain Grain Grain plant (gr) Geno- Area Width Area LengthWidth (based # type [cm{circumflex over ( )}2] [cm] [cm{circumflex over( )}2] [cm] [cm] on 6) 1 Line 1 91.624 5.728 0.806 1.228 0.824 655.556 2Line 2 85.058 5.584 0.753 1.167 0.810 657.500 3 Line 3 85.843 5.1510.708 1.092 0.814 491.667 4 Line 4 90.507 5.671 0.755 1.180 0.803641.111 5 Line 5 95.953 5.533 0.766 1.205 0.803 580.556 6 Line 6 72.4085.227 0.713 1.123 0.803 569.444 7 Line 7 74.032 5.221 0.714 1.139 0.791511.111 8 Line 8 76.534 5.328 0.753 1.134 0.837 544.444 9 Line 9 63.5994.605 0.582 1.042 0.703 633.333 10 Line 10 70.456 5.350 0.629 1.0950.721 558.333 11 Line 11 95.360 5.577 0.762 1.180 0.812 522.222 12 Line12 55.201 4.120 0.502 0.921 0.675 574.167 Table 30.

TABLE 31 Correlation between the expression level of selected genes ofsome embodiments of the invention in various tissues and the phenotypicperformance under normal conditions across maize varieties Gene Exp CorR P LYM346 D 1 0.90 0.002 LYM346 D 15 0.86 0.006 LYM346 D 1 0.84 0.009LYM346 D 15 0.80 0.016 LYM346 D 7 0.73 0.038 LYM346 D 16 0.72 0.046LYM346 F 9 −0.74 0.059 LYM346 F 5 −0.74 0.059 LYM346 F 4 −0.83 0.020LYM346 F 2 −0.86 0.013 LYM346 F 13 −0.86 0.013 LYM346 F 3 −0.86 0.012LYM346 F 7 −0.90 0.006 LYM346 F 16 −0.90 0.005 LYM346 F 10 −0.92 0.003LYM346 F 12 −0.93 0.002 LYM346 F 17 −0.95 0.001 LYM346 F 15 −0.95 0.001LYM349 E 16 0.91 0.001 LYM349 F 2 0.91 0.005 LYM349 E 16 0.91 0.001LYM349 E 1 0.90 0.001 LYM349 E 1 0.90 0.001 LYM349 E 2 0.90 0.001 LYM349A 4 0.89 0.008 LYM349 E 2 0.88 0.002 LYM349 A 2 0.88 0.010 LYM349 A 40.87 0.011 LYM349 E 15 0.87 0.003 LYM349 F 2 0.87 0.012 LYM349 E 7 0.870.003 LYM349 E 15 0.87 0.003 LYM349 A 7 0.86 0.012 LYM349 E 7 0.86 0.003LYM349 A 1 0.84 0.017 LYM349 E 5 0.84 0.005 LYM349 A 2 0.84 0.019 LYM349A 7 0.84 0.019 LYM349 F 11 0.83 0.021 LYM349 E 5 0.83 0.006 LYM349 A 150.82 0.023 LYM349 E 9 0.82 0.007 LYM349 F 1 0.82 0.025 LYM349 E 3 0.820.007 LYM349 E 9 0.81 0.008 LYM349 A 1 0.81 0.028 LYM349 A 12 0.81 0.028LYM349 E 6 0.80 0.009 LYM349 E 10 0.80 0.009 LYM349 A 15 0.80 0.030LYM349 E 3 0.80 0.010 LYM349 E 10 0.80 0.010 LYM349 F 3 0.79 0.033LYM349 A 16 0.79 0.034 LYM349 F 7 0.79 0.034 LYM349 E 12 0.79 0.011LYM349 A 17 0.79 0.036 LYM349 F 12 0.78 0.039 LYM349 E 11 0.78 0.013LYM349 E 6 0.78 0.014 LYM349 E 12 0.78 0.014 LYM349 F 11 0.77 0.042LYM349 A 16 0.77 0.043 LYM349 E 13 0.77 0.015 LYM349 A 17 0.77 0.043LYM349 A 12 0.77 0.044 LYM349 E 13 0.77 0.016 LYM349 F 7 0.77 0.045LYM349 E 11 0.76 0.017 LYM349 F 12 0.76 0.048 LYM349 E 4 0.75 0.019LYM349 E 4 0.75 0.020 LYM349 A 3 0.75 0.053 LYM349 F 9 0.73 0.060 LYM349F 15 0.73 0.064 LYM349 F 1 0.72 0.067 LYM349 F 3 0.72 0.069 LYM349 A 100.72 0.069 LYM349 F 5 0.72 0.069 LYM349 E 17 0.72 0.029 LYM349 E 17 0.710.031 LYM349 A 11 0.71 0.073 LYM349 F 4 0.71 0.076 LYM349 F 13 0.700.079 LYM349 F 13 0.70 0.080 LYM351 F 7 0.94 0.001 LYM351 J 10 0.940.002 LYM351 F 15 0.93 0.003 LYM351 F 16 0.92 0.003 LYM351 J 5 0.920.003 LYM351 J 9 0.91 0.004 LYM351 J 5 0.91 0.004 LYM351 J 13 0.91 0.004LYM351 J 16 0.91 0.005 LYM351 J 12 0.90 0.006 LYM351 J 10 0.90 0.006LYM351 J 9 0.89 0.007 LYM351 J 13 0.88 0.009 LYM351 F 12 0.87 0.011LYM351 F 1 0.86 0.012 LYM351 J 7 0.86 0.012 LYM351 H 12 0.86 0.001LYM351 J 15 0.86 0.013 LYM351 F 3 0.86 0.013 LYM351 F 10 0.85 0.017LYM351 J 1 0.84 0.017 LYM351 F 2 0.84 0.018 LYM351 A 7 0.84 0.018 LYM351J 16 0.84 0.019 LYM351 F 17 0.84 0.019 LYM351 H 4 0.83 0.003 LYM351 H 160.83 0.003 LYM351 F 11 0.83 0.020 LYM351 J 12 0.83 0.021 LYM351 J 110.83 0.022 LYM351 H 10 0.81 0.004 LYM351 F 9 0.81 0.027 LYM351 H 15 0.810.005 LYM351 H 7 0.81 0.005 LYM351 E 15 0.81 0.009 LYM351 F 6 0.80 0.029LYM351 H 5 0.80 0.005 LYM351 A 15 0.80 0.031 LYM351 H 1 0.80 0.005LYM351 E 16 0.80 0.010 LYM351 J 1 0.79 0.033 LYM351 F 4 0.79 0.034LYM351 F 13 0.79 0.035 LYM351 A 16 0.79 0.035 LYM351 H 2 0.78 0.007LYM351 A 3 0.77 0.042 LYM351 A 6 0.77 0.043 LYM351 J 11 0.77 0.044LYM351 J 7 0.77 0.045 LYM351 E 7 0.75 0.019 LYM351 J 6 0.75 0.051 LYM351E 2 0.75 0.019 LYM351 J 15 0.75 0.051 LYM351 E 17 0.75 0.020 LYM351 H 130.75 0.013 LYM351 J 3 0.75 0.055 LYM351 A 17 0.74 0.056 LYM351 F 5 0.740.058 LYM351 J 2 0.73 0.062 LYM351 A 2 0.73 0.063 LYM351 J 6 0.72 0.065LYM351 A 4 0.72 0.070 LYM351 A 1 0.72 0.071 LYM351 E 3 0.71 0.031 LYM351H 9 0.71 0.021 LYM351 H 17 0.71 0.021 LYM351 E 4 0.71 0.033 LYM351 J 170.70 0.077 LYM352 A 7 0.79 0.036 LYM352 A 3 0.77 0.042 LYM352 A 15 0.760.049 LYM352 A 17 0.76 0.049 LYM352 A 2 0.72 0.067 LYM352 A 16 0.710.071 LYM352 A 4 0.70 0.079 LYM354 F 13 0.90 0.006 LYM354 J 13 0.880.008 LYM354 F 5 0.86 0.013 LYM354 F 9 0.86 0.014 LYM354 J 3 0.85 0.015LYM354 F 10 0.83 0.020 LYM354 J 10 0.81 0.027 LYM354 J 12 0.81 0.029LYM354 J 2 0.79 0.034 LYM354 F 11 0.79 0.035 LYM354 F 12 0.78 0.039LYM354 J 9 0.78 0.039 LYM354 J 5 0.77 0.045 LYM354 J 11 0.77 0.045LYM354 F 3 0.73 0.062 LYM355 A 4 0.71 0.072 LYM355 D 18 −0.82 0.013LYM355 D 18 −0.83 0.011 LYM356 D 3 −0.73 0.038 LYM356 D 2 −0.84 0.009LYM359 D 1 0.88 0.004 LYM359 D 8 0.88 0.004 LYM359 D 18 0.85 0.008LYM359 D 7 0.83 0.010 LYM359 D 15 0.83 0.011 LYM359 D 16 0.78 0.022LYM359 D 11 0.75 0.034 LYM359 D 9 0.71 0.050 LYM359 D 2 0.70 0.052LYM359 F 8 −0.79 0.060 LYM360 A 7 0.93 0.002 LYM360 A 3 0.93 0.003LYM360 F 12 0.92 0.004 LYM360 A 2 0.91 0.004 LYM360 A 15 0.91 0.004LYM360 J 3 0.90 0.005 LYM360 A 12 0.90 0.006 LYM360 F 13 0.89 0.007LYM360 F 7 0.89 0.007 LYM360 F 10 0.89 0.008 LYM360 A 16 0.88 0.009LYM360 F 2 0.88 0.009 LYM360 A 17 0.88 0.009 LYM360 F 16 0.86 0.012LYM360 J 13 0.86 0.013 LYM360 F 3 0.86 0.013 LYM360 H 10 0.85 0.002LYM360 F 15 0.85 0.016 LYM360 J 12 0.84 0.017 LYM360 A 10 0.84 0.018LYM360 J 10 0.84 0.018 LYM360 F 5 0.83 0.021 LYM360 H 13 0.82 0.003LYM360 F 9 0.82 0.023 LYM360 H 12 0.82 0.004 LYM360 A 13 0.82 0.025LYM360 F 11 0.82 0.025 LYM360 H 5 0.81 0.004 LYM360 A 4 0.81 0.028LYM360 E 4 0.81 0.008 LYM360 J 2 0.80 0.029 LYM360 J 15 0.80 0.032LYM360 H 16 0.79 0.006 LYM360 J 7 0.79 0.035 LYM360 A 11 0.78 0.039LYM360 H 15 0.78 0.008 LYM360 J 17 0.77 0.042 LYM360 J 16 0.77 0.042LYM360 E 16 0.76 0.017 LYM360 F 17 0.76 0.047 LYM360 F 4 0.76 0.048LYM360 H 7 0.76 0.011 LYM360 A 9 0.74 0.055 LYM360 E 15 0.74 0.023LYM360 J 9 0.73 0.060 LYM360 H 2 0.73 0.016 LYM360 F 1 0.73 0.065 LYM360F 6 0.72 0.069 LYM360 J 11 0.71 0.072 LYM360 H 17 0.71 0.021 LYM360 H 40.71 0.022 LYM360 A 6 0.70 0.077 LYM360 A 1 0.70 0.078 LYM360 J 5 0.700.079 LYM360 A 5 0.70 0.080 LYM361 J 16 0.97 0.000 LYM361 J 15 0.950.001 LYM361 J 7 0.95 0.001 LYM361 A 15 0.94 0.002 LYM361 A 16 0.940.002 LYM361 F 16 0.94 0.002 LYM361 J 12 0.93 0.003 LYM361 A 4 0.920.003 LYM361 J 10 0.92 0.003 LYM361 A 7 0.90 0.006 LYM361 F 10 0.890.007 LYM361 A 17 0.88 0.009 LYM361 J 4 0.87 0.010 LYM361 F 7 0.87 0.011LYM361 J 17 0.87 0.011 LYM361 F 15 0.86 0.012 LYM361 J 13 0.86 0.013LYM361 F 12 0.85 0.016 LYM361 A 12 0.84 0.017 LYM361 A 10 0.84 0.019LYM361 F 6 0.84 0.019 LYM361 F 13 0.83 0.020 LYM361 J 3 0.82 0.023LYM361 F 5 0.82 0.024 LYM361 F 9 0.82 0.025 LYM361 J 2 0.80 0.031 LYM361J 9 0.79 0.034 LYM361 J 5 0.79 0.035 LYM361 J 6 0.77 0.042 LYM361 J 10.77 0.045 LYM361 A 1 0.76 0.049 LYM361 F 1 0.75 0.052 LYM361 F 4 0.750.054 LYM361 J 11 0.73 0.061 LYM361 A 13 0.73 0.065 LYM361 F 17 0.700.077 LYM361 F 11 0.70 0.078 LYM362 A 17 0.97 0.000 LYM362 A 15 0.960.001 LYM362 A 4 0.95 0.001 LYM362 A 7 0.90 0.005 LYM362 A 12 0.90 0.005LYM362 A 16 0.90 0.006 LYM362 H 17 0.89 0.001 LYM362 H 15 0.87 0.001LYM362 A 10 0.87 0.012 LYM362 A 2 0.86 0.013 LYM362 H 12 0.85 0.002LYM362 H 16 0.84 0.003 LYM362 H 10 0.83 0.003 LYM362 A 1 0.82 0.025LYM362 E 17 0.81 0.008 LYM362 H 7 0.81 0.004 LYM362 H 4 0.78 0.008LYM362 E 4 0.78 0.014 LYM362 A 13 0.77 0.044 LYM362 A 3 0.76 0.046LYM362 H 13 0.74 0.014 LYM362 H 3 0.73 0.017 LYM362 F 17 0.72 0.066LYM362 H 2 0.70 0.024 LYM364 A 16 0.87 0.010 LYM364 A 15 0.87 0.011LYM364 A 16 0.85 0.015 LYM364 A 15 0.85 0.016 LYM364 A 10 0.83 0.021LYM364 A 7 0.83 0.021 LYM364 A 17 0.82 0.024 LYM364 A 10 0.80 0.031LYM364 A 7 0.80 0.032 LYM364 A 4 0.79 0.033 LYM364 A 12 0.79 0.033LYM364 A 17 0.79 0.035 LYM364 A 12 0.79 0.036 LYM364 A 13 0.77 0.042LYM364 A 3 0.74 0.055 LYM364 A 13 0.71 0.072 LYM366 B 17 0.74 0.092LYM366 A 1 −0.71 0.075 LYM366 A 13 −0.77 0.041 LYM366 A 10 −0.85 0.016LYM366 A 3 −0.86 0.013 LYM366 A 2 −0.87 0.012 LYM366 A 12 −0.89 0.007LYM366 A 4 −0.90 0.006 LYM366 A 16 −0.91 0.005 LYM366 A 7 −0.93 0.003LYM366 A 15 −0.97 0.000 LYM366 A 17 −0.98 0.000 LYM368 F 15 0.98 0.000LYM368 F 17 0.97 0.000 LYM368 F 15 0.97 0.000 LYM368 A 15 0.96 0.000LYM368 A 17 0.96 0.001 LYM368 F 17 0.96 0.001 LYM368 A 17 0.95 0.001LYM368 F 15 0.95 0.001 LYM368 F 16 0.95 0.001 LYM368 A 17 0.95 0.001LYM368 E 17 0.94 0.000 LYM368 J 4 0.94 0.002 LYM368 A 7 0.94 0.002LYM368 E 17 0.94 0.000 LYM368 F 17 0.94 0.002 LYM368 H 15 0.94 0.000LYM368 F 7 0.93 0.002 LYM368 J 4 0.93 0.002 LYM368 J 17 0.93 0.002LYM368 J 4 0.93 0.002 LYM368 J 17 0.93 0.002 LYM368 J 17 0.93 0.003LYM368 A 4 0.93 0.003 LYM368 A 15 0.93 0.003 LYM368 F 12 0.93 0.003LYM368 H 17 0.93 0.000 LYM368 F 16 0.92 0.003 LYM368 F 10 0.92 0.003LYM368 A 4 0.92 0.003 LYM368 H 15 0.92 0.000 LYM368 H 17 0.92 0.000LYM368 F 7 0.91 0.004 LYM368 E 15 0.91 0.001 LYM368 A 16 0.91 0.004LYM368 H 15 0.91 0.000 LYM368 J 15 0.90 0.005 LYM368 J 15 0.90 0.005LYM368 H 17 0.90 0.000 LYM368 E 15 0.90 0.001 LYM368 E 15 0.90 0.001LYM368 A 7 0.90 0.006 LYM368 H 7 0.89 0.000 LYM368 H 16 0.89 0.001LYM368 F 16 0.89 0.007 LYM368 E 4 0.89 0.001 LYM368 J 15 0.89 0.007LYM368 E 17 0.89 0.001 LYM368 H 16 0.89 0.001 LYM368 F 7 0.89 0.008LYM368 F 12 0.89 0.008 LYM368 E 16 0.89 0.002 LYM368 F 4 0.88 0.009LYM368 E 4 0.87 0.002 LYM368 E 7 0.87 0.002 LYM368 E 16 0.87 0.002LYM368 F 10 0.87 0.011 LYM368 F 4 0.87 0.011 LYM368 H 10 0.86 0.001LYM368 A 12 0.86 0.013 LYM368 A 16 0.86 0.013 LYM368 A 15 0.86 0.013LYM368 F 3 0.86 0.013 LYM368 E 7 0.86 0.003 LYM368 E 4 0.86 0.003 LYM368H 10 0.86 0.002 LYM368 H 16 0.86 0.002 LYM368 F 13 0.85 0.014 LYM368 F12 0.85 0.014 LYM368 H 7 0.85 0.002 LYM368 A 2 0.85 0.015 LYM368 E 120.85 0.004 LYM368 H 12 0.85 0.002 LYM368 F 4 0.85 0.017 LYM368 H 7 0.840.002 LYM368 E 16 0.84 0.004 LYM368 J 7 0.84 0.018 LYM368 J 16 0.840.018 LYM368 J 16 0.84 0.018 LYM368 E 10 0.84 0.005 LYM368 E 7 0.840.005 LYM368 J 7 0.83 0.020 LYM368 E 10 0.83 0.005 LYM368 F 10 0.830.021 LYM368 A 2 0.83 0.021 LYM368 H 10 0.83 0.003 LYM368 A 3 0.83 0.022LYM368 F 2 0.82 0.023 LYM368 F 3 0.82 0.023 LYM368 A 12 0.82 0.023LYM368 H 12 0.82 0.004 LYM368 E 12 0.82 0.007 LYM368 E 2 0.82 0.007LYM368 J 16 0.82 0.025 LYM368 E 10 0.81 0.007 LYM368 J 7 0.81 0.026LYM368 A 4 0.81 0.026 LYM368 A 10 0.81 0.027 LYM368 F 3 0.81 0.027LYM368 E 12 0.81 0.009 LYM368 H 12 0.80 0.005 LYM368 A 3 0.80 0.029LYM368 A 7 0.80 0.030 LYM368 F 2 0.80 0.031 LYM368 A 2 0.80 0.032 LYM368A 3 0.79 0.034 LYM368 H 13 0.79 0.006 LYM368 F 2 0.78 0.038 LYM368 A 10.78 0.038 LYM368 F 13 0.78 0.038 LYM368 E 2 0.78 0.013 LYM368 H 13 0.770.009 LYM368 F 9 0.77 0.044 LYM368 E 2 0.77 0.016 LYM368 H 4 0.76 0.011LYM368 E 3 0.76 0.018 LYM368 H 3 0.76 0.012 LYM368 A 10 0.76 0.050LYM368 A 16 0.75 0.050 LYM368 J 12 0.75 0.051 LYM368 E 13 0.75 0.020LYM368 H 2 0.75 0.013 LYM368 J 12 0.75 0.053 LYM368 H 4 0.75 0.013LYM368 A 12 0.75 0.053 LYM368 F 5 0.75 0.053 LYM368 H 4 0.74 0.014LYM368 F 1 0.74 0.058 LYM368 H 13 0.74 0.015 LYM368 F 13 0.74 0.059LYM368 J 12 0.73 0.061 LYM368 E 13 0.73 0.026 LYM368 H 1 0.73 0.017LYM368 H 3 0.73 0.017 LYM368 H 9 0.72 0.018 LYM368 H 3 0.72 0.019 LYM368A 13 0.72 0.071 LYM368 F 11 0.71 0.072 LYM368 E 13 0.71 0.032 LYM368 J10 0.71 0.073 LYM368 A 1 0.71 0.075 LYM368 E 3 0.70 0.034 LYM368 H 20.70 0.023 LYM368 J 10 0.70 0.079 LYM369 J 17 0.81 0.026 LYM369 J 150.79 0.035 LYM369 J 4 0.77 0.044 LYM369 J 1 0.75 0.053 LYM369 J 7 0.720.066 LYM369 J 16 0.70 0.077 LYM369 E 10 −0.72 0.027 LYM369 E 4 −0.730.026 LYM369 D 16 −0.74 0.035 LYM369 D 16 −0.74 0.035 LYM369 E 3 −0.760.017 LYM369 D 1 −0.76 0.028 LYM369 E 12 −0.76 0.017 LYM369 D 1 −0.770.024 LYM369 D 18 −0.79 0.019 LYM369 D 18 −0.80 0.016 LYM369 E 2 −0.820.007 LYM369 E 16 −0.84 0.005 LYM369 E 3 −0.85 0.004 LYM369 E 7 −0.860.003 LYM369 E 15 −0.89 0.001 LYM369 E 17 −0.91 0.001 LYM370 E 3 −0.740.022 LYM370 E 10 −0.77 0.016 LYM370 E 2 −0.79 0.011 LYM370 F 2 −0.800.029 LYM370 E 12 −0.82 0.007 LYM370 E 16 −0.85 0.004 LYM370 E 7 −0.860.003 LYM370 E 4 −0.89 0.001 LYM370 E 15 −0.90 0.001 LYM370 E 17 −0.940.000 LYM372 H 15 0.93 0.000 LYM372 J 17 0.92 0.003 LYM372 A 15 0.910.004 LYM372 H 16 0.91 0.000 LYM372 A 4 0.91 0.005 LYM372 H 7 0.90 0.000LYM372 A 16 0.89 0.007 LYM372 H 17 0.89 0.001 LYM372 A 17 0.89 0.008LYM372 F 17 0.87 0.012 LYM372 F 4 0.86 0.013 LYM372 A 7 0.86 0.013LYM372 H 12 0.85 0.002 LYM372 H 10 0.85 0.002 LYM372 F 15 0.85 0.016LYM372 J 15 0.84 0.018 LYM372 J 4 0.82 0.025 LYM372 F 16 0.81 0.028LYM372 A 12 0.80 0.032 LYM372 H 4 0.79 0.006 LYM372 A 10 0.79 0.036LYM372 E 17 0.78 0.012 LYM372 F 7 0.77 0.042 LYM372 H 3 0.77 0.009LYM372 H 13 0.77 0.010 LYM372 J 16 0.75 0.051 LYM372 E 15 0.75 0.020LYM372 J 7 0.74 0.057 LYM372 E 4 0.73 0.026 LYM372 H 2 0.73 0.017 LYM372E 16 0.72 0.028 LYM372 F 12 0.72 0.069 LYM372 E 2 0.71 0.031 LYM372 F 100.71 0.077 LYM372 E 7 0.70 0.035 LYM374 F 16 0.92 0.003 LYM374 B 17 0.920.010 LYM374 B 17 0.91 0.011 LYM374 F 7 0.90 0.006 LYM374 F 15 0.880.009 LYM374 F 16 0.88 0.009 LYM374 F 6 0.88 0.010 LYM374 H 10 0.870.001 LYM374 F 15 0.86 0.014 LYM374 H 15 0.84 0.002 LYM374 J 17 0.840.019 LYM374 H 12 0.83 0.003 LYM374 H 16 0.83 0.003 LYM374 F 10 0.830.021 LYM374 H 4 0.82 0.003 LYM374 F 10 0.82 0.023 LYM374 F 7 0.82 0.025LYM374 F 12 0.81 0.027 LYM374 H 17 0.80 0.005 LYM374 F 12 0.79 0.034LYM374 F 3 0.79 0.034 LYM374 F 9 0.79 0.035 LYM374 H 10 0.79 0.007LYM374 F 1 0.79 0.036 LYM374 H 7 0.78 0.008 LYM374 J 15 0.78 0.040LYM374 H 5 0.78 0.008 LYM374 F 13 0.77 0.044 LYM374 H 13 0.76 0.010LYM374 F 11 0.76 0.049 LYM374 J 4 0.75 0.050 LYM374 F 1 0.75 0.051LYM374 J 2 0.75 0.052 LYM374 F 17 0.75 0.052 LYM374 F 17 0.75 0.053LYM374 F 4 0.74 0.055 LYM374 H 4 0.74 0.014 LYM374 H 1 0.74 0.015 LYM374F 13 0.73 0.060 LYM374 F 9 0.73 0.061 LYM374 H 15 0.73 0.016 LYM374 F 50.72 0.067 LYM374 H 16 0.72 0.019 LYM374 F 4 0.72 0.069 LYM374 J 1 0.720.069 LYM374 F 5 0.72 0.071 LYM374 H 12 0.71 0.021 LYM374 J 7 0.70 0.078LYM374 H 17 0.70 0.024 LYM374 B 6 −0.75 0.087 LYM374 B 6 −0.76 0.078LYM376 F 12 0.94 0.002 LYM376 F 17 0.93 0.002 LYM376 F 15 0.93 0.002LYM376 F 3 0.93 0.002 LYM376 F 17 0.93 0.002 LYM376 F 2 0.92 0.003LYM376 F 10 0.92 0.004 LYM376 F 2 0.90 0.005 LYM376 F 7 0.90 0.006LYM376 F 12 0.89 0.006 LYM376 F 13 0.89 0.007 LYM376 F 15 0.89 0.007LYM376 F 16 0.87 0.010 LYM376 F 3 0.87 0.012 LYM376 F 10 0.86 0.013LYM376 A 12 0.84 0.017 LYM376 A 10 0.83 0.021 LYM376 F 7 0.83 0.021LYM376 F 13 0.83 0.022 LYM376 H 17 0.82 0.003 LYM376 A 2 0.81 0.028LYM376 A 13 0.81 0.029 LYM376 F 16 0.80 0.030 LYM376 F 9 0.77 0.041LYM376 F 4 0.77 0.042 LYM376 A 5 0.77 0.043 LYM376 F 4 0.76 0.046 LYM376A 15 0.76 0.048 LYM376 E 13 0.76 0.018 LYM376 F 11 0.76 0.048 LYM376 F 50.75 0.050 LYM376 A 17 0.75 0.053 LYM376 J 13 0.74 0.058 LYM376 H 170.73 0.016 LYM376 J 2 0.73 0.061 LYM376 J 3 0.73 0.061 LYM376 J 12 0.730.064 LYM376 A 1 0.72 0.070 LYM376 A 7 0.71 0.073 LYM376 J 10 0.71 0.073LYM376 A 16 0.71 0.075 LYM376 E 10 0.70 0.034 LYM376 A 4 0.70 0.079LYM376 J 18 −0.71 0.073 LYM377 A 17 0.98 0.000 LYM377 A 15 0.97 0.000LYM377 A 17 0.96 0.001 LYM377 A 15 0.96 0.001 LYM377 A 7 0.93 0.002LYM377 A 7 0.93 0.002 LYM377 A 16 0.92 0.004 LYM377 A 4 0.91 0.005LYM377 A 3 0.90 0.005 LYM377 A 16 0.90 0.005 LYM377 A 12 0.90 0.006LYM377 A 2 0.88 0.009 LYM377 A 12 0.88 0.009 LYM377 A 10 0.86 0.013LYM377 A 4 0.85 0.014 LYM377 A 3 0.85 0.016 LYM377 A 2 0.84 0.017 LYM377A 10 0.84 0.018 LYM377 F 15 0.84 0.019 LYM377 F 16 0.83 0.020 LYM377 F15 0.83 0.021 LYM377 J 13 0.83 0.021 LYM377 F 7 0.83 0.022 LYM377 F 160.83 0.022 LYM377 F 7 0.82 0.023 LYM377 J 13 0.81 0.026 LYM377 F 12 0.810.027 LYM377 F 17 0.81 0.029 LYM377 A 13 0.80 0.030 LYM377 F 10 0.800.032 LYM377 J 10 0.80 0.032 LYM377 H 13 0.80 0.006 LYM377 F 17 0.800.032 LYM377 F 12 0.79 0.033 LYM377 H 13 0.79 0.006 LYM377 J 10 0.790.034 LYM377 F 4 0.78 0.037 LYM377 H 10 0.78 0.007 LYM377 H 10 0.780.008 LYM377 F 3 0.77 0.041 LYM377 F 10 0.77 0.044 LYM377 E 12 0.760.018 LYM377 F 13 0.76 0.049 LYM377 J 12 0.76 0.049 LYM377 F 4 0.750.050 LYM377 A 13 0.75 0.051 LYM377 F 3 0.75 0.052 LYM377 J 12 0.750.053 LYM377 J 5 0.74 0.056 LYM377 J 16 0.73 0.061 LYM377 A 1 0.73 0.062LYM377 H 5 0.73 0.018 LYM377 J 5 0.72 0.065 LYM377 F 13 0.72 0.067LYM377 J 9 0.72 0.067 LYM377 H 5 0.72 0.019 LYM377 J 9 0.71 0.077 LYM377F 2 0.70 0.077 LYM377 F 2 0.70 0.077 LYM378 F 9 0.88 0.010 LYM378 F 110.87 0.010 LYM378 F 6 0.87 0.011 LYM378 F 5 0.85 0.015 LYM378 F 1 0.760.046 LYM378 F 13 0.74 0.059 LYM378 G 1 −0.71 0.047 LYM378 G 6 −0.720.044 LYM378 G 16 −0.80 0.018 LYM379 D 15 0.75 0.031 LYM379 D 1 0.750.033 LYM380 E 15 −0.71 0.033 LYM380 E 16 −0.71 0.033 LYM380 E 1 −0.720.028 LYM380 E 4 −0.74 0.022 LYM380 E 16 −0.74 0.022 LYM380 B 7 −0.750.087 LYM380 E 4 −0.76 0.018 LYM380 B 7 −0.76 0.081 LYM380 E 1 −0.760.018 LYM382 F 2 0.95 0.001 LYM382 F 2 0.92 0.003 LYM382 F 2 0.92 0.004LYM382 F 3 0.88 0.009 LYM382 F 17 0.87 0.010 LYM382 F 3 0.86 0.013LYM382 F 17 0.85 0.016 LYM382 F 3 0.83 0.020 LYM382 F 17 0.83 0.022LYM382 G 1 0.80 0.017 LYM382 F 12 0.79 0.034 LYM382 F 15 0.79 0.036LYM382 F 15 0.78 0.040 LYM382 F 12 0.77 0.042 LYM382 F 7 0.77 0.045LYM382 G 1 0.76 0.030 LYM382 F 12 0.74 0.058 LYM382 F 7 0.73 0.060LYM382 F 15 0.73 0.063 LYM382 F 11 0.72 0.069 LYM382 F 10 0.71 0.072LYM382 F 13 0.70 0.077 LYM382 G 16 0.70 0.052 LYM382 J 11 −0.70 0.079LYM382 D 10 −0.70 0.051 LYM382 D 13 −0.70 0.051 LYM382 J 5 −0.71 0.077LYM382 A 9 −0.71 0.077 LYM382 D 12 −0.71 0.050 LYM382 J 5 −0.71 0.075LYM382 A 9 −0.71 0.075 LYM382 J 11 −0.71 0.072 LYM382 J 6 −0.72 0.070LYM382 J 6 −0.72 0.069 LYM382 J 6 −0.72 0.068 LYM382 J 5 −0.72 0.067LYM382 D 13 −0.72 0.042 LYM382 J 9 −0.73 0.063 LYM382 J 9 −0.73 0.063LYM382 J 4 −0.73 0.063 LYM382 D 12 −0.73 0.039 LYM382 A 1 −0.73 0.061LYM382 D 10 −0.73 0.039 LYM382 D 11 −0.73 0.039 LYM382 D 16 −0.74 0.035LYM382 A 1 −0.74 0.055 LYM382 J 9 −0.74 0.055 LYM382 J 4 −0.76 0.049LYM382 D 16 −0.76 0.030 LYM382 D 11 −0.76 0.029 LYM382 D 5 −0.77 0.027LYM382 A 1 −0.77 0.044 LYM382 J 4 −0.77 0.042 LYM382 D 5 −0.77 0.024LYM382 J 2 −0.77 0.041 LYM382 A 13 −0.79 0.035 LYM382 J 2 −0.79 0.035LYM382 A 13 −0.79 0.034 LYM382 J 17 −0.79 0.033 LYM382 A 13 −0.79 0.033LYM382 D 9 −0.80 0.018 LYM382 J 2 −0.80 0.032 LYM382 D 18 −0.80 0.016LYM382 D 7 −0.81 0.014 LYM382 D 9 −0.82 0.013 LYM382 D 18 −0.82 0.013LYM382 J 13 −0.82 0.023 LYM382 J 13 −0.82 0.023 LYM382 J 17 −0.83 0.021LYM382 J 17 −0.83 0.021 LYM382 J 10 −0.84 0.018 LYM382 J 13 −0.84 0.018LYM382 D 7 −0.84 0.009 LYM382 A 3 −0.85 0.016 LYM382 J 3 −0.85 0.015LYM382 J 10 −0.85 0.015 LYM382 J 15 −0.85 0.014 LYM382 J 12 −0.85 0.014LYM382 J 3 −0.86 0.014 LYM382 A 3 −0.86 0.013 LYM382 A 2 −0.86 0.013LYM382 A 2 −0.86 0.013 LYM382 A 3 −0.86 0.013 LYM382 J 16 −0.86 0.013LYM382 A 2 −0.86 0.013 LYM382 J 10 −0.86 0.012 LYM382 A 10 −0.87 0.012LYM382 A 10 −0.87 0.012 LYM382 A 10 −0.87 0.011 LYM382 J 7 −0.87 0.011LYM382 J 3 −0.87 0.011 LYM382 J 12 −0.87 0.011 LYM382 J 12 −0.88 0.009LYM382 J 15 −0.88 0.008 LYM382 J 15 −0.89 0.008 LYM382 J 16 −0.89 0.008LYM382 J 16 −0.89 0.008 LYM382 J 7 −0.89 0.007 LYM382 J 7 −0.89 0.007LYM382 A 12 −0.91 0.005 LYM382 A 4 −0.91 0.005 LYM382 A 12 −0.91 0.005LYM382 A 12 −0.91 0.005 LYM382 A 4 −0.91 0.004 LYM382 A 4 −0.93 0.003LYM382 A 16 −0.93 0.002 LYM382 A 16 −0.93 0.002 LYM382 A 16 −0.94 0.002LYM382 A 7 −0.95 0.001 LYM382 A 7 −0.95 0.001 LYM382 A 17 −0.95 0.001LYM382 A 17 −0.95 0.001 LYM382 A 17 −0.96 0.001 LYM382 A 7 −0.96 0.001LYM382 A 15 −0.97 0.000 LYM382 A 15 −0.97 0.000 LYM382 A 15 −0.98 0.000LYM383 F 9 0.88 0.009 LYM383 F 11 0.88 0.009 LYM383 F 5 0.86 0.013LYM383 F 6 0.85 0.017 LYM383 F 1 0.77 0.042 LYM383 F 13 0.74 0.056LYM383 E 12 −0.71 0.034 LYM383 E 15 −0.71 0.032 LYM383 E 13 −0.72 0.029LYM383 E 10 −0.75 0.021 LYM383 E 17 −0.75 0.020 LYM384 J 16 0.93 0.002LYM384 J 15 0.92 0.003 LYM384 J 10 0.91 0.004 LYM384 J 12 0.90 0.006LYM384 J 7 0.89 0.008 LYM384 J 4 0.88 0.008 LYM384 J 17 0.85 0.015LYM384 J 13 0.83 0.021 LYM384 J 5 0.78 0.040 LYM384 J 1 0.74 0.055LYM384 J 9 0.73 0.060 LYM384 J 2 0.72 0.069 LYM384 J 3 0.70 0.078 LYM384D 7 −0.72 0.042 LYM384 D 8 −0.74 0.038 LYM384 D 18 −0.88 0.004 LYM385 A5 0.94 0.001 LYM385 A 1 0.94 0.002 LYM385 A 9 0.94 0.002 LYM385 A 6 0.890.007 LYM385 A 11 0.89 0.008 LYM385 A 16 0.88 0.008 LYM385 F 6 0.880.010 LYM385 A 10 0.88 0.010 LYM385 F 16 0.87 0.011 LYM385 J 1 0.870.011 LYM385 J 5 0.87 0.012 LYM385 A 13 0.86 0.012 LYM385 A 7 0.86 0.013LYM385 A 12 0.86 0.014 LYM385 F 1 0.86 0.014 LYM385 F 5 0.85 0.017LYM385 F 7 0.82 0.024 LYM385 F 10 0.81 0.027 LYM385 F 9 0.81 0.028LYM385 J 9 0.80 0.030 LYM385 F 12 0.78 0.037 LYM385 A 15 0.78 0.038LYM385 E 4 0.77 0.016 LYM385 E 12 0.76 0.017 LYM385 F 13 0.76 0.047LYM385 J 11 0.76 0.047 LYM385 F 15 0.76 0.049 LYM385 F 4 0.75 0.050LYM385 J 13 0.73 0.060 LYM385 J 12 0.73 0.063 LYM385 J 10 0.73 0.064LYM385 A 2 0.71 0.071 LYM385 F 11 0.71 0.071 LYM385 H 5 0.71 0.023LYM386 B 17 0.91 0.011 LYM386 G 10 0.83 0.012 LYM386 H 13 0.79 0.007LYM386 G 16 0.79 0.021 LYM386 H 13 0.78 0.007 LYM386 H 9 0.77 0.009LYM386 H 11 0.76 0.011 LYM386 G 15 0.75 0.032 LYM386 G 5 0.75 0.033LYM386 G 16 0.74 0.036 LYM386 G 13 0.73 0.038 LYM386 H 5 0.73 0.016LYM386 H 5 0.73 0.016 LYM386 H 12 0.72 0.020 LYM386 G 9 0.72 0.046LYM386 H 10 0.71 0.020 LYM386 H 9 0.71 0.021 LYM386 G 10 0.71 0.047LYM387 J 15 0.94 0.002 LYM387 J 16 0.91 0.004 LYM387 J 17 0.91 0.005LYM387 J 10 0.91 0.005 LYM387 J 12 0.90 0.005 LYM387 J 7 0.89 0.007LYM387 J 13 0.85 0.017 LYM387 J 3 0.83 0.021 LYM387 J 16 0.83 0.022LYM387 J 3 0.82 0.024 LYM387 J 10 0.82 0.025 LYM387 J 4 0.81 0.026LYM387 J 13 0.81 0.026 LYM387 J 7 0.81 0.028 LYM387 J 12 0.79 0.033LYM387 J 15 0.79 0.034 LYM387 F 6 0.79 0.035 LYM387 J 2 0.78 0.039LYM387 J 6 0.75 0.050 LYM387 J 9 0.74 0.058 LYM387 J 9 0.73 0.064 LYM387J 5 0.72 0.065 Table 31. Provided are the correlations (R) between theexpression levels yield improving genes and their homologs in varioustissues [Expression (Exp) sets] and the phenotypic performance [yield,biomass, growth rate and/or vigor components (Correlation vector (Cor))]under normal conditions across maize varieties. P = p value.

Example 8 Gene Cloning and Generation of Binary Vectors for Plant

Expression To validate their role in improving plant yield, oil content,seed yield, biomass, growth rate, fiber yield, fiber quality, ABST, NUEand/or vigor, selected genes were over-expressed in plants, as follows.

Cloning Strategy

Selected genes from those listed in Examples 1-7 hereinabove were clonedinto binary vectors for the generation of transgenic plants. Forcloning, the full-length open reading frame (ORF) was first identified.In case of ORF-EST clusters and in some cases already published mRNAsequences were analyzed to identify the entire open reading frame bycomparing the results of several translation algorithms to knownproteins from other plant species. To clone the full-length cDNAs,reverse transcription (RT) followed by polymerase chain reaction (PCR;RT-PCR) was performed on total RNA extracted from leaves, flowers,siliques or other plant tissues, growing under normal and differenttreated conditions. Total RNA was extracted as described in “GENERALEXPERIMENTAL AND BIOINFORMATICS METHODS” above. Production of cDNA andPCR amplification was performed using standard protocols describedelsewhere (Sambrook J., E. F. Fritsch, and T. Maniatis. 1989. MolecularCloning. A Laboratory Manual., 2nd Ed. Cold Spring Harbor LaboratoryPress, New York.) which are well known to those skilled in the art. PCRproducts are purified using PCR purification kit (Qiagen). In case wherethe entire coding sequence was not found, RACE kit from Invitrogen(RACE=R apid A mplification of cDNA E nds) was used to access the fullcDNA transcript of the gene from the RNA samples described above. RACEproducts were cloned into high copy vector followed by sequencing ordirectly sequenced.

The information from the RACE procedure was used for cloning of the fulllength ORF of the corresponding genes.

In case genomic DNA was cloned, the genes were amplified by direct PCRon genomic DNA extracted from leaf tissue using the DNAeasy kit (QiagenCat. No. 69104).

Usually, 2 sets of primers were synthesized for the amplification ofeach gene from a cDNA or a genomic sequence; an external set of primersand an internal set (nested PCR primers). When needed (e.g., when thefirst PCR reaction does not result in a satisfactory product forsequencing), an additional primer (or two) of the nested PCR primerswere used.

To facilitate cloning of the cDNAs/genomic sequences, a 8-12 bpextension was added to the 5′ of each primer. The primer extensionincludes an endonuclease restriction site. The restriction sites wereselected using two parameters: (a). The site does not exist in the cDNAsequence; and (b). The restriction sites in the forward and reverseprimers were designed such that the digested cDNA was inserted in thesense formation into the binary vector utilized for transformation.

Each digested PCR product was inserted into a high copy vector pUC 19(New England BioLabs Inc], or into plasmids originating from thisvector. In some cases the undigested PCR product was inserted intopCR-Blunt II-TOPO (Invitrogen).

Sequencing of the amplified PCR products was performed, using ABI 377sequencer (Amersham Biosciences Inc). In some cases, after confirmingthe sequences of the cloned genes, the cloned cDNA was introduced into amodified pGI binary vector containing the At6669 promoter via digestionwith appropriate restriction endonucleases. In any case the insert wasfollowed by single copy of the NOS terminator (SEQ ID NO:8092). Thedigested products and the linearized plasmid vector are ligated using T4DNA ligase enzyme (Roche, Switzerland).

High copy plasmids containing the cloned genes were digested with therestriction endonucleases (New England BioLabs Inc) according to thesites designed in the primers and cloned into binary vectors as shown inTable 32, below.

Several DNA sequences of the selected genes were synthesized by acommercial supplier GeneArt [Hypertext Transfer Protocol://World WideWeb (dot) geneart (dot) coma Synthetic DNA was designed in silico.Suitable restriction enzymes sites were added to the cloned sequences atthe 5′ end and at the 3′ end to enable later cloning into the pQFNcbinary vector downstream of the At6669 promoter (SEQ ID NO: 4668).

Binary Vectors Used for Cloning:

The plasmid pPI is constructed by inserting a synthetic poly-(A) signalsequence, originating from pGL3 basic plasmid vector (Promega, Acc NoU47295; by 4658-4811) into the HindIII restriction site of the binaryvector pBI101.3 (Clontech, Acc. No. U12640). pGI (pBXYN) is similar topPI, but the original gene in the backbone, the GUS gene, is replaced bythe GUS-Intron gene followed by the NOS terminator (SEQ ID NO:4664)(Vancanneyt. G, et al MGG 220, 245-50, 1990). pGI was used in the pastto clone the polynucleotide sequences, initially under the control of35S promoter [Odell, J T, et al. Nature 313, 810-812 (28 Feb. 1985); SEQID NO:4666].

The modified pGI vectors [pQXNc (FIG. 8); or pQFN (FIG. 2), pQFNc (FIG.2) or pQYN_6669 (FIG. 1)] are modified versions of the pGI vector inwhich the cassette is inverted between the left and right borders so thegene and its corresponding promoter are close to the right border andthe NPTII gene is close to the left border.

At6669, the Arabidopsis thaliana promoter sequence (SEQ ID NO:4668) wasinserted in the modified pGI binary vector, upstream to the clonedgenes, followed by DNA ligation and binary plasmid extraction frompositive E. coli colonies, as described above.

Colonies were analyzed by PCR using the primers covering the insertwhich were designed to span the introduced promoter and gene. Positiveplasmids were identified, isolated and sequenced.

Genes which were cloned by the present inventors are provided in Table32 below.

TABLE 32 Genes cloned in High copy number plasmids Gene High copyPrimers used SEQ Polyn. SEQ Polyp. SEQ Name plasmid Organism ID NOs: IDNO: ID NO: LYM297 Topo B ARABIDOPSIS Arabidopsis thaliana Kondara 4670,4805, 4909, 5042 2 482 LYM337 203 — LYM425 206 — LYM428 208 — LYM434 213— LYM46 289 481 LYM298 pUC19c ARABIDOPSIS Arabidopsis thaliana Columbiawt 4806, 5043 290 483 LYM299 pUC19c ARABIDOPSIS Arabidopsis thalianaColumbia wt 4671, 4807, 4910, 5044 291 484 LYM300 pUC19c BARLEY Hordeumvulgare L. Manit 4808, 5045 292 485 LYM301 pUC19c BARLEY Hordeum vulgareL. Manit 4672, 4809, 4911, 5046 293 486 LYM302 Topo B BARLEY Hordeumvulgare L. Manit 4810, 5047 294 487 LYM303 pUC19c BARLEY Hordeum vulgareL. Manit 4673, 4811, 4912, 5048 295 728 LYM305 pUC19c BARLEY Hordeumvulgare L. Manit 4674, 4812, 4913, 4913 296 729 LYM306 pUC19c BARLEYHordeum vulgare L. Manit 4675, 4675, 4914, 5049 297 491 LYM308 pUC19cBARLEY Hordeum vulgare L. Spontaneum 4676, 4676, 4915, 4915 298 493LYM309 pUC19c BARLEY Hordeum vulgare L. Manit 4677, 4813, 4916, 5050 299494 LYM310 pUC19c BARLEY Hordeum vulgare L. Manit 4678, 4814, 4678, 5051300 495 LYM312 pUC19c BARLEY Hordeum vulgare L. Spontaneum 4679, 4815,4917, 5052 301 730 LYM313 pUC19c BARLEY Hordeum vulgare L. Manit 4680,4816, 4918, 5053 302 497 LYM314 pUC19c BARLEY Hordeum vulgare L.Spontaneum 4681, 4919 303 498 LYM315 304 499 LYM316 305 500 LYM317 TopoB BARLEY Hordeum vulgare L. Manit 4682, 4682, 4920, 4920 306 501 LYM318pUC19c BARLEY Hordeum vulgare L. Manit 4683, 4817, 4921, 5054 307 502LYM319 pUC19c BARLEY Hordeum vulgare L. Manit 4818, 5055 308 503 LYM320pUC19c BARLEY Hordeum vulgare L. Manit 4819, 5056 309 731 LYM321 pUC19cBARLEY Hordeum vulgare L. Manit 4684, 4684, 4922, 5057 310 732 LYM322pUC19c BARLEY Hordeum vulgare L. Manit 4685, 4685, 4923, 5058 311 733LYM323 pUC19c BARLEY Hordeum vulgare L. Manit 4820, 5059 312 734 LYM324pUC19c BARLEY Hordeum vulgare L. Spontaneum 4686, 4924 313 735 LYM327pUC19c BARLEY Hordeum vulgare L. Manit 4687, 4821, 4925, 5060 314 736LYM328 pUC19c BARLEY Hordeum vulgare L. Manit 4688, 4822, 4926, 4926 315737 LYM329 pUC19c BARLEY Hordeum vulgare L. Manit 4823, 5061 316 738LYM330 pUC19c BARLEY Hordeum vulgare L. Manit 4824, 5062 317 739 LYM331pUC19c BARLEY Hordeum vulgare L. Manit 4689, 4927 318 740 LYM332 pUC19cBARLEY Hordeum vulgare L. Manit 4690, 4825, 4928, 5063 319 741 LYM333320 516 LYM334 pUC19c BARLEY Hordeum vulgare L. Manit 4691, 4826, 4929,5064 321 517 LYM335 322 518 LYM336 pUC19c BARLEY Hordeum vulgare L.Manit 4827, 5065 323 742 LYM338 pUC19c BARLEY Hordeum vulgare L.Spontaneum 4828, 5066 324 693 LYM339 pUC19c BARLEY Hordeum vulgare L.Manit 4692, 4829, 4930, 5067 325 743 LYM340 pUC19c BRACHYPODIUMBrachypodiums distachyon 4693, 4830, 4931, 5068 326 744 ND LYM341 Topo BBRACHYPODIUM Brachypodiums distachyon 4694, 4932 327 523 ND LYM343pUC19c WHEAT Triticum aestivum L. 4695, 4933 328 745 LYM344 directly toCOTTON Gossypium barbadense Pima 4696, 4831, 4934, 5069 329 746 binaryLYM345 Topo B COTTON Gossypium barbadense Pima 4697, 4697, 4935, 5070330 747 LYM346 pUC19c MAIZE Zea mays L. B73 4698, 4832, 4936, 5071 331748 LYM348 pUC19c MAIZE Zea mays L. B73 4699, 4833, 4937, 5072 332 749LYM349 pUC19c MAIZE Zea mays L. B73 4700, 4700, 4938, 4938 333 530LYM350 334 531 LYM351 pUC19c MAIZE Zea mays L. B73 4701, 4834, 4939,5073 335 532 LYM352 336 533 LYM353 pUC19c WHEAT Triticum aestivum L. ND4702, 4835, 4940, 5074 337 750 LYM354 pUC19c MAIZE Zea mays L. B73 4703,4836, 4941, 5075 338 751 LYM355 Topo B MAIZE Zea mays L. B73 4704, 4704,4942, 5076 339 752 LYM356 pUC19c MAIZE Zea mays L. B73 4705, 4837, 4943,5077 340 537 LYM357 341 538 LYM359 Topo B MAIZE Zea mays L. B73 4706,4838, 4944, 5078 342 539 LYM360 343 540 LYM361 directly to MAIZE Zeamays L. B73 4707, 4839, 4945, 5079 344 541 binary LYM362 pUC19c MAIZEZea mays L. B73 4708, 4840, 4946, 4946 345 542 LYM363 Topo B MAIZE Zeamays L. B73 4709, 4841, 4947, 5080 346 753 LYM364 pUC19c MAIZE Zea maysL. B73 4710, 4842, 4948, 5081 347 754 LYM365 pUC19c MAIZE Zea mays L.B73 4711, 4711, 4949, 5082 348 545 LYM366 pUC19c MAIZE Zea mays L. B734712, 4843, 4950, 5083 349 755 LYM367 Topo B MAIZE Zea mays L. B73 4713,4951 350 756 LYM369 pUC19c MAIZE Zea mays L. B73 4714, 4844, 4952, 5084351 757 LYM370 Topo B MAIZE Zea mays L. B73 4715, 4845, 4953, 5085 352758 LYM371 Topo B MAIZE Zea mays L. B73 4716, 4846, 4954, 5086 353 759LYM372 pUC19c MAIZE Zea mays L. B73 4717, 4847, 4955, 5087 354 760LYM373 pUC19c MAIZE Zea mays L. B73 4718, 4848, 4956, 5088 355 761LYM374 pUC19c MAIZE Zea mays L. B73 4719, 4849, 4957, 4957 356 554LYM375 directly to MAIZE Zea mays L. B73 4720, 4850, 4958, 5089 357 762binary LYM376 pUC19c MAIZE Zea mays L. B73 4721, 4851, 4959, 5090 358556 LYM377 pUC19c MAIZE Zea mays L. B73 4722, 4852, 4960, 5091 359 557LYM378 pUC19c MAIZE Zea mays L. B73 4723, 4723, 4961, 5092 360 558LYM379 361 559 LYM380 Topo B MAIZE Zea mays L. B73 4724, 4853, 4962,5093 362 560 LYM381 Topo B MAIZE Zea mays L. B73 4725, 4725, 4963, 4963363 763 LYM382 pUC19c MAIZE Zea mays L. B73 4726, 4854, 4964, 4964 364764 LYM384 365 564 LYM385 pUC19c MAIZE Zea mays L. B73 4727, 4855, 4965,5094 366 765 LYM386 367 566 LYM387 pUC19c MAIZE Zea mays L. B73 4728,4856, 4966, 5095 368 766 LYM388 pUC19c MAIZE Zea mays L. B73 4729, 4857,4967, 4967 369 568 LYM389 370 569 LYM390 pUC19c RICE Oryza sativa L.Indica TEBBONET 4730, 4858, 4968, 5096 371 570 LYM391 372 571 LYM392 373572 LYM393 pUC19c RICE Oryza sativa L. Indica TEBBONET 4731, 4859, 4969,5097 374 573 LYM394 375 574 LYM395 376 575 LYM396 pUC19c RICE Oryzasativa L. Indica TEBBONET 4732, 4732, 4970, 5098 377 576 LYM398 378 578LYM399 pUC19c RICE Oryza sativa L. Indica TEBBONET 4733, 4860, 4971,5099 379 579 LYM400 380 580 LYM401 pUC19c RICE Oryza sativa L. IndicaTEBBONET 4734, 4972 381 767 LYM402 382 582 LYM403 383 583 LYM404 Topo BRICE Oryza sativa L. Indica TEBBONET 4735, 4861, 4973, 5100 384 584LYM405 385 585 LYM406 Topo B RICE Oryza sativa L. Indica TEBBONET 4736,4862, 4974, 5101 386 586 LYM407 Topo B RICE Oryza sativa L. IndicaTEBBONET 4863, 5102 387 587 LYM409 pUC19c RICE Oryza sativa L. IndicaTEBBONET 4737, 4864, 4975, 5103 388 589 LYM410 Topo B RICE Oryza sativaL. Indica TEBBONET 4738, 4976 389 768 LYM413 Topo B RICE Oryza sativa L.Indica TEBBONET 4865, 5104 390 593 LYM414 pUC19c RICE Oryza sativa L.Indica TEBBONET 4739, 4977 391 769 LYM415 pUC19c RICE Oryza sativa L.Indica TEBBONET 4740, 4740, 4978, 5105 392 595 LYM416 pUC19c RICE Oryzasativa L. Indica TEBBONET 4741, 4741, 4979, 5106 393 596 LYM417 394 597LYM418 Topo B RICE Oryza sativa L. Indica TEBBONET 4742, 4866, 4980,5107 395 598 LYM419 pUC19c SORGHUM Sorghum bicolor ND 4743, 4981 396 599LYM421 397 600 LYM423 pUC19c SORGHUM Sorghum bicolor ND 4800, 4907,5037, 5037 398 601 LYM424 Topo B SORGHUM Sorghum bicolor ND 4744, 4867,4982, 4982 399 770 LYM427 400 603 LYM433 pUC19c SORGHUM Sorghum bicolorND 4745, 4868, 4983, 5108 401 604 LYM435 pUC19c SORGHUM Sorghum bicolorND 4746, 4984 402 605 LYM436 Topo B SORGHUM Sorghum bicolor ND 4747,4985 403 606 LYM437 Topo B SORGHUM Sorghum bicolor ND 4748, 4869, 4986,5109 404 607 LYM438 pUC19c SORGHUM Sorghum bicolor ND 4870, 5110 405 608LYM439 406 609 LYM440 Topo B SORGHUM Sorghum bicolor ND 4749, 4749,4987, 5111 407 610 LYM441 pUC19c SORGHUM Sorghum bicolor ND 4750, 4871,4988, 5112 408 771 LYM442 Topo B SORGHUM Sorghum bicolor ND 4751, 4872,4989, 5113 409 612 LYM443 pUC19c SORGHUM Sorghum bicolor ND 4752, 4873,4990, 5114 410 613 LYM444 pUC19c SORGHUM Sorghum bicolor ND 4753, 4753,4991, 4991 411 772 LYM445 pUC19c SORGHUM Sorghum bicolor ND 4754, 4992412 773 LYM446 pUC19c SORGHUM Sorghum bicolor ND 4755, 4755, 4993, 5115413 616 LYM447 pUC19c SORGHUM Sorghum bicolor ND 4756, 4874, 4994, 4994414 617 LYM448 pUC19c SORGHUM Sorghum bicolor ND 4757, 4995 415 618LYM449 Topo B SORGHUM Sorghum bicolor ND 4875, 5116 416 619 LYM450 417620 LYM451 pUC19c SORGHUM Sorghum bicolor ND 4758, 4876, 4996, 5117 418621 LYM452 419 622 LYM453 pUC19c SORGHUM Sorghum bicolor ND 4759, 4877,4997, 5118 420 774 LYM454 pUC19c SORGHUM Sorghum bicolor ND 4760, 4760,4998, 4998 421 624 LYM455 Topo B SORGHUM Sorghum bicolor ND 4761, 4999422 625 LYM456 pUC19c SORGHUM Sorghum bicolor ND 4878, 5119 423 626LYM457 424 627 LYM458 Topo B SORGHUM Sorghum bicolor ND 4879, 5120 425628 LYM460 pUC19c SORGHUM Sorghum bicolor ND 4762, 4880, 5000, 5121 426775 LYM461 Topo B SORGHUM Sorghum bicolor ND 4763, 4881, 5001, 5122 427630 LYM463 pUC19c SORGHUM Sorghum bicolor ND 4764, 4764, 5002, 5123 428776 LYM464 pUC19c SORGHUM Sorghum bicolor ND 4765, 4765, 5003, 5124 429632 LYM465 pUC19c SORGHUM Sorghum bicolor ND 4766, 4882, 5004, 5125 430777 LYM466 Topo B SORGHUM Sorghum bicolor ND 4767, 4883, 5005, 5126 431778 LYM467 Topo B SORGHUM Sorghum bicolor ND 4768, 5006 432 635 LYM468433 636 LYM471 Topo B SORGHUM Sorghum bicolor ND 4769, 4884, 5007, 5127434 779 LYM472 pUC19c SORGHUM Sorghum bicolor ND 4770, 5008 435 780LYM473 directly to SORGHUM Sorghum bicolor ND 4771, 4885, 5009, 5128 436639 binary LYM474 pUC19c SORGHUM Sorghum bicolor ND 4772, 4886, 5010,5129 437 640 LYM475 pUC19c SORGHUM Sorghum bicolor ND 4773, 5011 438 781LYM476 pUC19c SORGHUM Sorghum bicolor ND 4774, 4774, 5012, 5130 439 642LYM477 pUC19d SORGHUM Sorghum bicolor ND 4775, 4887, 5013, 5013 440 643LYM478 441 644 LYM480 pUC19c SORGHUM Sorghum bicolor ND 4776, 4888,4776, 5131 442 646 LYM481 Topo B SORGHUM Sorghum bicolor ND 4777, 5014443 782 LYM483 pUC19c SORGHUM Sorghum bicolor ND 4778, 4778, 5015, 5132444 783 LYM484 445 649 LYM485 446 650 LYM486 Topo B SORGHUM Sorghumbicolor ND 4779, 4889, 5016, 5133 447 651 LYM487 pUC19c SORGHUM Sorghumbicolor ND 4780, 4890, 5017, 5134 448 652 LYM488 pUC19c SORGHUM Sorghumbicolor ND 4781, 4891, 5018, 5135 449 784 LYM489 pUC19c SORGHUM Sorghumbicolor ND 4892, 5136 450 654 LYM490 451 655 LYM491 Topo B SORGHUMSorghum bicolor ND 4782, 4893, 5019, 5137 452 656 LYM492 pUC19c SORGHUMSorghum bicolor ND 4783, 4783, 5020, 5138 453 657 LYM493 Topo B SORGHUMSorghum bicolor ND 4784, 4894, 5021, 5139 454 785 LYM494 pUC19c SORGHUMSorghum bicolor ND 4895, 5140 455 659 LYM495 pUC19d SORGHUM Sorghumbicolor ND 4785, 4785, 5022, 5141 456 660 LYM496 Topo B SORGHUM Sorghumbicolor ND 4786, 4896, 5023, 5142 457 786 LYM497 458 662 LYM498 Topo BSORGHUM Sorghum bicolor ND 4787, 4897, 5024, 5143 459 663 LYM499 Topo BSORGHUM Sorghum bicolor ND 4788, 4788, 5025, 5144 460 787 LYM500 Topo BSORGHUM Sorghum bicolor ND 4789, 4898, 5026, 5026 461 788 LYM501 Topo BSORGHUM Sorghum bicolor ND 4899, 5145 462 789 LYM502 pUC19d SORGHUMSorghum bicolor ND 4790, 5027 463 667 LYM503 pUC19c SORGHUM Sorghumbicolor ND 4791, 4900, 5028, 5146 464 668 LYM504 pUC19c SORGHUM Sorghumbicolor ND 4792, 4792, 5029, 5029 465 669 LYM505 Topo B SORGHUM Sorghumbicolor ND 4793, 4901, 5030, 5147 466 670 LYM506 Topo B SORGHUM Sorghumbicolor ND 4794, 4902, 5031, 5148 467 671 LYM507 Topo B SORGHUM Sorghumbicolor ND 4903, 5149 468 672 LYM509 pUC19c SORGHUM Sorghum bicolor ND4795, 4904, 5032, 5150 469 674 LYM510 Topo B WHEAT Triticum aestivum L.ND 4796, 4796, 5033, 5151 470 790 LYM304_H3 471 676 LYM307_H7 pUC19cSORGHUM Sorghum bicolor ND 4802, 4802, 5039, 5039 472 791 LYM326_H4 473678 LYM368_H4 pUC19c SORGHUM Sorghum bicolor ND 4803, 4908, 5040, 5155474 679 LYM397_H2 Topo B SORGHUM Sorghum bicolor ND 4804, 4804, 5041,5041 475 792 LYM311 pUC19c BARLEY Hordeum vulgare L. Spontaneum 4905,5152 476 — LYM325 pUC19c BARLEY Hordeum vulgare L. Manit 4797, 4797,5034, 5153 477 — LYM420 Topo B SORGHUM Sorghum bicolor ND 4798, 5035 478— LYM422 Topo B SORGHUM Sorghum bicolor ND 4799, 4906, 5036, 5036 479 —LYM432 pUC19c SORGHUM Sorghum bicolor ND 4801, 4801, 5038, 5154 480 —Table 32. Provided are the genes which were cloned in high copyplasmids, along with the primers used for cloning, the organisms fromwhich the genes were cloned and the resulting polynucleotide (“polyn.”)and polypeptide (“polyp.”) sequences of the cloned genes.

Example 9 Transforming Agrobacterium Tumefaciens Cells with BinaryVectors Harboring Putative Genes

Each of the binary vectors described in Example 8 above were used totransform Agrobacterium cells. Two additional binary constructs, havinga GUS/Luciferase reporter gene replacing the selected gene (positioneddownstream of the At6669 promoter) were used as negative controls.

The binary vectors were introduced to Agrobacterium tumefaciens GV301,or LB4404 competent cells (about 10⁹ cells/mL) by electroporation. Theelectroporation was performed using a MicroPulser electroporator(Biorad), 0.2 cm cuvettes (Biorad) and EC-2 electroporation program(Biorad). The treated cells were cultured in LB liquid medium at 28° C.for 3 hours, then plated over LB agar supplemented with gentamycin (50mg/L; for Agrobacterium strains GV301) or streptomycin (300 mg/L; forAgrobacterium strain LB4404) and kanamycin (50 mg/L) at 28° C. for 48hours. Abrobacterium colonies which developed on the selective mediawere analyzed by PCR using the primers which were designed to span theinserted sequence in the pPI plasmid. The resulting PCR products wereisolated and sequenced as described in Example 8 above, to verify thatthe correct nucleotide sequences were properly introduced to theAgrobacterium cells.

Example 10 Producing Transgenic Arabidopsis Plants Expressing SelectedGenes According to Some Embodiments of the Invention

Materials and Experimental Methods

Plant Transformation—

The Arabidopsis thaliana var Columbia (T₀ plants) were transformedaccording to the Floral Dip procedure [Clough S J, Bent A F. (1998)Floral dip: a simplified method for Agrobacterium-mediatedtransformation of Arabidopsis thaliana. Plant J. 16(6): 735-43; andDesfeux C, Clough S J, Bent A F. (2000) Female reproductive tissues arethe primary targets of Agrobacterium-mediated transformation by theArabidopsis floral-dip method. Plant Physiol. 123(3): 895-904] withminor modifications. Briefly, Arabidopsis thaliana Columbia (Co10) T₀plants were sown in 250 ml pots filled with wet peat-based growth mix.The pots were covered with aluminum foil and a plastic dome, kept at 4°C. for 3-4 days, then uncovered and incubated in a growth chamber at18-24° C. under 16/8 hours light/dark cycles. The T₀ plants were readyfor transformation six days before anthesis.

Single colonies of Agrobacterium carrying the binary vectors harboringthe yield genes were cultured in LB medium supplemented with kanamycin(50 mg/L) and gentamycin (50 mg/L). The cultures were incubated at 28°C. for 48 hours under vigorous shaking and centrifuged at 4000 rpm for 5minutes. The pellets comprising Agrobacterium cells were resuspended ina transformation medium which contained half-strength (2.15 g/L)Murashige-Skoog (Duchefa); 0.044 μM benzylamino purine (Sigma); 112 μg/LB5 Gambourg vitamins (Sigma); 5% sucrose; and 0.2 ml/L Silwet L-77 (OSISpecialists, CT) in double-distilled water, at pH of 5.7.

Transformation of T₀ plants was performed by inverting each plant intoan Agrobacterium suspension such that the above ground plant tissue wassubmerged for 3-5 seconds. Each inoculated T₀ plant was immediatelyplaced in a plastic tray, then covered with clear plastic dome tomaintain humidity and was kept in the dark at room temperature for 18hours to facilitate infection and transformation. Transformed(transgenic) plants were then uncovered and transferred to a greenhousefor recovery and maturation. The transgenic T₀ plants were grown in thegreenhouse for 3-5 weeks until siliques were brown and dry, then seedswere harvested from plants and kept at room temperature until sowing.

For generating T₁ and T₂ transgenic plants harboring the genes, seedscollected from transgenic T₀ plants were surface-sterilized by soakingin 70% ethanol for 1 minute, followed by soaking in 5% sodiumhypochlorite and 0.05% triton for 5 minutes. The surface-sterilizedseeds were thoroughly washed in sterile distilled water then placed onculture plates containing half-strength Murashig-Skoog (Duchefa); 2%sucrose; 0.8% plant agar; 50 mM kanamycin; and 200 mM carbenicylin(Duchefa). The culture plates were incubated at 4° C. for 48 hours thentransferred to a growth room at 25° C. for an additional week ofincubation. Vital T₁ Arabidopsis plants were transferred to a freshculture plates for another week of incubation. Following incubation theT₁ plants were removed from culture plates and planted in growth mixcontained in 250 ml pots. The transgenic plants were allowed to grow ina greenhouse to maturity. Seeds harvested from T₁ plants were culturedand grown to maturity as T₂ plants under the same conditions as used forculturing and growing the T₁ plants.

Example 11 Evaluation of Transgenic Arabidopsis for Seed Yield and PlantGrowth Rate Under Normal Conditions in Greenhouse ASSAYS (GH-SM Assays)

Assay 1: Seed Yield Plant Biomass and Plant Growth Rate Under NormalGreenhouse Conditions—

This assay follows seed yield production, the biomass formation and therosette area growth of plants grown in the greenhouse at non-limitingnitrogen growth conditions. Transgenic Arabidopsis seeds were sown inagar media supplemented with ½ MS medium and a selection agent(Kanamycin). The T₂ transgenic seedlings were then transplanted to 1.7trays filled with peat and perlite in a 1:1 ratio. The trays wereirrigated with a solution containing 6 mM inorganic nitrogen in the formof KNO₃ with 1 mM KH₂PO₄, 1 mM MgSO₄, 2 mM CaCl₂ and microelements. Allplants were grown in the greenhouse until mature seeds. Seeds wereharvested, extracted and weight. The remaining plant biomass (the aboveground tissue) was also harvested, and weighted immediately or followingdrying in oven at 50° C. for 24 hours.

Each construct was validated at its T₂ generation. Transgenic plantstransformed with a construct conformed by an empty vector carrying theAt6669 promoter and the selectable marker was used as control.

The plants were analyzed for their overall size, growth rate, flowering,seed yield, 1,000-seed weight, dry matter and harvest index (HI-seedyield/dry matter). Transgenic plants performance was compared to controlplants grown in parallel under the same conditions. Mock-transgenicplants expressing the uidA reporter gene (GUS-Intron) or with no gene atall, under the same promoter were used as control.

The experiment was planned in nested randomized plot distribution. Foreach gene of the invention three to five independent transformationevents were analyzed from each construct.

Digital Imaging—

A laboratory image acquisition system, which consists of a digitalreflex camera (Canon EOS 300D) attached with a 55 mm focal length lens(Canon EF-S series), mounted on a reproduction device (Kaiser RS), whichincludes 4 light units (4×150 Watts light bulb) was used for capturingimages of plant samples.

The image capturing process was repeated every 2 days starting from day1 after transplanting till day 15. Same camera, placed in a custom madeiron mount, was used for capturing images of larger plants sawn in whitetubs in an environmental controlled greenhouse. The tubs are squareshape include 1.7 liter trays. During the capture process, the tubs wereplaced beneath the iron mount, while avoiding direct sun light andcasting of shadows.

An image analysis system was used, which consists of a personal desktopcomputer (Intel P4 3.0 GHz processor) and a public domain program—ImageJ1.39 [Java based image processing program which was developed at theU.S. National Institutes of Health and freely available on the internetat Hypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/]. Imageswere captured in resolution of 10 Mega Pixels (3888×2592 pixels) andstored in a low compression JPEG (Joint Photographic Experts Groupstandard) format. Next, analyzed data was saved to text files andprocessed using the JMP statistical analysis software (SAS institute).

Leaf Analysis—

Using the digital analysis leaves data was calculated, including leafnumber, rosette area, rosette diameter, and leaf blade area.

Vegetative growth rate: the relative growth rate (RGR) of leaf number[formula X (described above)], rosette area (formula XII), plot coverage(formula XIII) and harvest index (formula IV) was calculated with theindicated formulas.

Relative growth rate of rosette area=Regression coefficient of rosettearea along time course.  Formula XII:

Relative growth rate of plot coverage=Regression coefficient of plotcoverage along time course.  Formula XIII

Seeds Average Weight—

At the end of the experiment all seeds were collected. The seeds werescattered on a glass tray and a picture was taken. Using the digitalanalysis, the number of seeds in each sample was calculated.

Dry Weight and Seed Yield—

On about day 80 from sowing, the plants were harvested and left to dryat 30° C. in a drying chamber. The biomass and seed weight of each plotwere measured and divided by the number of plants in each plot. Dryweight=total weight of the vegetative portion above ground (excludingroots) after drying at 30° C. in a drying chamber; Seed yield perplant=total seed weight per plant (gr). 1000 seed weight (the weight of1000 seeds) (gr.).

The harvest index (HI) was calculated using Formula IV as describedabove.

Oil Percentage in Seeds—

At the end of the experiment all seeds from each plot were collected.Seeds from 3 plots were mixed grounded and then mounted onto theextraction chamber. 210 ml of n-Hexane (Cat No. 080951 Biolab Ltd.) wereused as the solvent. The extraction was performed for 30 hours at mediumheat 50° C. Once the extraction has ended the n-Hexane was evaporatedusing the evaporator at 35° C. and vacuum conditions. The process wasrepeated twice. The information gained from the Soxhlet extractor(Soxhlet, F. Die gewichtsanalytische Bestimmung des Milchfettes,Polytechnisches J. (Dingler's) 1879, 232, 461) was used to create acalibration curve for the Low Resonance NMR. The content of oil of allseed samples was determined using the Low Resonance NMR (MARANUltra—Oxford Instrument) and its MultiQuant software package

Silique Length Analysis—

On day 50 from sowing, 30 siliques from different plants in each plotwere sampled in block A. The chosen siliques were green-yellow in colorand were collected from the bottom parts of a grown plant's stem. Adigital photograph was taken to determine silique's length.

Statistical Analyses—

To identify outperforming genes and constructs, results from theindependent transformation events tested were analyzed separately. Datawas analyzed using Student's t-test and results are consideredsignificant if the p value was less than 0.1. The JMP statisticssoftware package was used (Version 5.2.1, SAS Institute Inc., Cary,N.C., USA).

Tables 33-37 summarize the observed phenotypes of transgenic plantsexogenously expressing the gene constructs using the GH-SM Assays.

TABLE 33 Genes showing improved plant performance at Normal growthconditions under regulation of At6669 promoter Inflorescence Dry Weight[mg] Flowering Emergence Gene P- % P- % P- % Name Event # Ave. Val.Incr. Ave. Val. Incr. Ave. Val. Incr. LYM509 62200.4 1077.4 0.05 14 — —— — — — LYM507 62272.9 1056.9 0.28 12 — — — — — — LYM500 62368.2 1053.80.11 12 — — — — — — LYM498 62078.2 1096.9 L 17 — — — — — — LYM49662269.9 1006.0 0.06 7 — — — — — — LYM492 62136.6 1080.6 L 15 — — — — — —LYM492 62137.4 1063.1 0.07 13 — — — — — — LYM492 62140.2 1021.9 0.19 9 —— — — — — LYM487 62151.1 1112.9 0.25 18 — — — — — — LYM477 62052.51048.0 0.02 11 — — — — — — LYM466 62212.2 1111.2 0.27 18 — — — — — —LYM407 62142.6 1100.6 0.01 17 — — — — — — LYM407 62142.8 1008.1 0.04 7 —— — — — — LYM404 62243.12 1050.6 0.19 12 — — — — — — LYM399 62085.41055.0 0.01 12 — — — — — — LYM382 62061.1 1024.4 0.03 9 — — — — — —LYM343 62458.2 1086.9 0.19 15 — — — — — — LYM329 62419.5 1128.1 0.19 20— — — — — — LYM323 62356.5 1008.1 0.23 7 — — — — — — LYM323 62358.41095.0 0.15 16 — — — — — — LYM322 62334.5 1093.8 L 16 — — — — — — LYM32262336.1 1038.8 0.07 10 — — — — — — LYM317 62251.12 1063.3 0.19 13 — — —— — — LYM302 62258.1 1133.2 L 20 — — — — — — CONT. — 941.4 — — — — — — —— LYM503 61581.5 — — — — — — 27.2 0.09 −3 LYM493 61967.6 1190.7 0.06 1533.6 0.08 −3 27.1 0.25 −3 LYM493 61969.8 1123.4 0.09 9 — — — — — —LYM480 61960.6 — — — — — — 27.4 0.11 −2 LYM473 61786.1 1128.1 0.25 9 — —— — — — LYM456 61589.6 — — — — — — 27.2 0.21 −3 LYM440 61936.6 1117.50.23 8 33.3 0.19 −3 26.8 0.13 −4 LYM440 61939.6 — — — 34.0 0.24 −1 — — —LYM415 61598.5 1131.2 0.21 10 — — — — — — LYM415 61602.6 — — — 34.1 0.19−1 — — — LYM415 61602.8 — — — 33.6 0.26 −3 26.5 L −5 LYM409 61998.6 — —— 33.9 0.01 −2 — — — LYM393 61610.6 — — — 32.7 0.18 −5 25.6 0.10 −8LYM380 61824.2 1206.2 0.26 17 — — — — — — LYM380 61825.2 1170.6 0.06 13— — — — — — LYM380 61825.5 1239.4 0.10 20 — — — — — — LYM380 61828.31164.4 0.03 13 — — — — — — LYM377 61593.5 — — — — — — 27.1 0.01 −3LYM377 61594.8 — — — — — — 27.2 0.02 −3 LYM376 61835.3 — — — — — — 27.30.05 −2 LYM375 61758.3 — — — 32.8 0.22 −5 26.7 L −4 LYM366 61910.6 — — —33.7 0.07 −2 27.4 0.07 −2 LYM361 61797.1 — — — 33.9 0.25 −2 — — — LYM34661616.9 — — — 33.6 0.25 −3 26.8 0.11 −4 LYM346 61618.4 — — — 33.8 0.02−2 27.3 0.04 −2 LYM344 61788.2 — — — 31.9 0.08 −7 25.1 0.02 −10 LYM34461790.1 — — — 33.8 0.05 −2 26.9 0.23 −4 LYM344 61791.1 — — — 33.7 L −227.2 0.02 −3 LYM334 61943.12 — — — 33.8 L −2 — — — LYM327 61846.1 1130.10.06 10 — — — 27.0 0.21 −3 LYM327 61847.1 — — — 33.9 0.29 −2 — — —LYM318 61623.2 — — — 33.3 0.04 −3 26.6 0.19 −5 LYM318 61624.1 — — — — —— 25.9 0.20 −7 LYM318 61625.2 — — — — — — 27.4 0.07 −2 LYM313 61629.1 —— — 33.7 0.07 −2 27.3 0.05 −2 LYM313 61631.3 — — — 33.1 0.03 −4 26.00.26 −7 LYM310 61636.2 — — — 33.5 0.09 −3 27.0 0.01 −3 LYM310 61637.4 —— — 34.0 0.24 −1 27.3 0.05 −2 LYM300 61749.4 — — — 33.7 L −2 27.0 0.02−3 LYM300 61750.3 — — — 33.7 0.07 −2 — — — LYM300 61750.4 1152.5 0.12 12— — — — — — LYM299 61807.4 — — — 33.6 0.20 −3 27.2 0.05 −2 LYM29961808.4 — — — — — — 27.2 0.02 −3 CONT. — 1031.9 — — 34.5 — — 27.9 — —Table 33. “CONT.”—Control; “Ave.”—Average; “% Incr.” = % increment;“p-val.”—p-value, L—p < 0.01. The transgenes were under thetranscriptional regulation of the new At6669 promoter (SEQ ID NO: 4668).

TABLE 34 Genes showing improved plant performance at Normal growthconditions under regulation of At6669 promoter Leaf Blade Area PlotCoverage [cm²] Leaf Number [cm²] Gene P- % P- % P- % Name Event # Ave.Val. Incr. Ave. Val. Incr. Ave. Val. Incr. LYM509 62202.1 — — — 10.20.20 7 — — — LYM507 62272.9 2.5 0.20 9 10.5 0.14 10 51.0 0.07 19 LYM50762273.1 — — — 9.8 0.02 2 — — — LYM507 62273.12 2.5 0.09 8 — — — — — —LYM506 62208.4 — — — 9.7 0.20 1 46.3 0.13 8 LYM505 62279.5 — — — 9.80.05 3 46.5 0.17 9 LYM499 62098.1 — — — 9.6 0.28 1 — — — LYM498 62078.2— — — 9.9 0.17 3 45.2 0.21 6 LYM494 62414.4 — — — 9.9 0.26 4 — — —LYM494 62414.5 2.8 0.13 20 — — — 54.9 0.12 28 LYM492 62136.6 2.6 0.19 11— — — 48.1 0.08 13 LYM489 61834.1 — — — 9.9 0.01 4 — — — LYM466 62212.32.5 0.06 8 — — — 48.5 L 13 LYM454 62194.2 — — — 9.6 0.28 1 45.3 0.14 6LYM454 62198.4 2.7 0.07 16 — — — 50.4 0.19 18 LYM438 62186.3 2.6 0.06 13— — — — — — LYM437 62406.3 — — — 9.7 0.20 1 — — — LYM437 62406.4 3.0 L29 10.2 0.28 7 58.1 0.10 36 LYM424 62284.9 2.4 0.28 5 — — — — — — LYM41862226.1 — — — 9.9 0.17 3 — — — LYM407 62142.6 — — — 10.0 L 5 — — —LYM407 62145.1 — — — 10.0 L 5 — — — LYM404 62243.12 — — — 9.7 0.20 1 — —— LYM404 62244.12 — — — 9.9 0.26 4 — — — LYM399 62085.2 2.5 0.06 8 10.10.08 6 49.6 L 16 LYM387 62102.4 2.5 0.10 8 — — — — — — LYM373 62159.12.8 L 19 10.2 0.20 7 53.1 0.01 24 LYM356 62089.3 — — — 9.8 0.02 2 — — —LYM356 62092.3 2.5 0.04 9 9.9 0.26 4 45.9 0.12 7 LYM329 62417.1 — — —10.0 0.11 5 — — — LYM329 62418.3 2.5 0.18 8 — — — 45.9 0.12 7 LYM32962419.5 2.7 0.23 17 9.7 0.20 1 — — — LYM323 62359.3 — — — 10.1 0.08 6 —— — LYM322 62332.3 2.5 0.29 7 10.1 L 6 — — — LYM322 62336.1 2.9 L 26 — —— 57.1 0.06 34 LYM317 62251.12 — — — 10.0 0.11 5 — — — LYM302 62258.1 —— — 9.9 L 3 — — — CONT. — 2.3 — — 9.5 — — 42.7 — — LYM503 61581.5 — — —11.6 0.26 2 — — — LYM493 61966.4 — — — 12.4 L 10 — — — LYM493 61968.8 —— — 11.6 0.17 3 — — — LYM493 61969.8 — — — 11.6 0.26 2 — — — LYM48061961.1 — — — 11.9 0.08 6 — — — LYM480 61962.8 — — — 12.3 0.02 9 — — —LYM473 61783.2 — — — 11.6 0.22 3 — — — LYM473 61784.2 — — — 11.6 0.26 2— — — LYM473 61784.3 — — — 12.1 0.09 7 76.8 0.19 8 LYM458 61812.4 — — —12.3 0.10 9 78.6 0.24 11 LYM458 61816.4 — — — 11.6 0.26 2 — — — LYM45661588.5 — — — 11.6 0.26 2 — — — LYM456 61588.7 — — — 11.8 0.05 5 78.10.11 10 LYM456 61589.6 — — — 11.9 0.18 5 — — — LYM453 61985.4 3.8 0.1910 — — — 80.7 0.09 14 LYM442 61978.7 3.7 0.20 7 — — — 76.4 0.21 8 LYM44261983.7 — — — 11.8 0.07 4 — — — LYM440 61936.6 — — — — — — 76.4 0.21 7LYM440 61937.6 3.6 0.26 6 11.8 0.13 5 78.2 0.11 10 LYM415 61598.5 4.1 L18 — — — 86.6 0.04 22 LYM415 61598.7 — — — 11.7 0.12 4 — — — LYM41561602.8 — — — — — — 75.5 0.28 6 LYM413 61819.3 — — — 12.1 L 7 — — —LYM396 61902.8 — — — 11.7 0.23 4 — — — LYM393 61610.6 4.1 L 19 — — —84.8 0.03 19 LYM393 61610.8 — — — 11.8 0.13 5 — — — LYM380 61828.3 4.00.13 16 12.0 0.03 6 85.8 0.02 21 LYM377 61593.5 — — — — — — 77.0 0.30 8LYM377 61594.8 — — — 12.1 0.21 7 82.7 0.23 16 LYM376 61835.2 — — — 12.30.29 9 — — — LYM376 61836.1 3.9 0.05 12 11.8 0.11 4 85.9 L 21 LYM37262002.1 — — — — — — 77.6 0.26 9 LYM366 61906.9 3.9 0.04 13 — — — 78.90.09 11 LYM366 61910.6 — — — 11.7 0.23 4 82.1 0.12 16 LYM366 61910.8 — —— 11.6 0.22 3 — — — LYM361 61795.1 — — — 11.6 0.17 3 — — — LYM36161795.2 3.7 0.24 9 — — — 81.4 0.15 15 LYM361 61797.1 — — — 11.7 0.12 4 —— — LYM354 61800.4 — — — 11.8 0.05 5 — — — LYM354 61804.3 — — — 12.2 L 980.1 0.23 13 LYM346 61616.15 — — — 11.6 0.26 2 — — — LYM344 61788.2 — —— 12.1 0.21 7 — — — LYM344 61788.4 — — — 12.0 0.01 6 — — — LYM34461790.1 3.7 0.21 7 — — — 78.0 0.14 10 LYM334 61943.12 — — — 11.9 0.08 6— — — LYM334 61947.7 — — — 11.9 0.18 5 — — — LYM330 61841.4 — — — 11.80.29 5 — — — LYM330 61842.4 — — — 11.9 0.05 5 — — — LYM327 61846.1 3.90.04 12 12.0 0.12 6 84.7 0.07 19 LYM327 61847.1 — — — 12.1 0.21 7 — — —LYM320 61851.1 — — — 12.1 0.05 7 — — — LYM320 61854.2 4.2 L 22 — — —92.0 L 29 LYM319 61920.6 3.7 0.25 6 — — — — — — LYM319 61921.5 — — —11.6 0.26 2 — — — LYM318 61624.1 3.9 0.26 12 — — — — — — LYM313 61629.13.7 0.22 6 — — — 75.8 0.26 7 LYM313 61631.3 3.8 0.09 10 — — — 81.4 0.0415 LYM301 61913.4 — — — 11.6 0.26 2 — — — LYM300 61750.3 — — — 12.2 0.179 — — — LYM300 61750.4 — — — 12.0 0.03 6 — — — LYM299 61807.4 — — — 11.70.23 4 — — — CONT. — 3.4 — — 11.3 — — 71.1 — — Table 34.“CONT.”—Control; “Ave.”—Average; “% Incr.” = % increment;“p-val.”—p-value, L—p < 0.01. The transgenes were under thetranscriptional regulation of the new At6669 promoter (SEQ ID NO: 4668).

TABLE 35 Genes showing improved plant performance at Normal growthconditions under regulation of At6669 promoter RGR Of Leaf RGR Of PlotRGR Of Rosette Number Coverage Diameter Gene Event P- % P- % P- % Name #Ave. Val. Incr. Ave. Val. Incr. Ave. Val. Incr. LYM507 62272.9 0.7 0.1318 6.3 0.13 19 — — — LYM498 62078.2 0.7 0.28 13 — — — — — — LYM49462414.5 0.7 0.19 18 6.8 0.03 29 0.4 0.02 19 LYM492 62136.6 — — — 6.30.18 18 0.4 0.25 10 LYM487 62149.1 0.8 0.07 23 6.2 0.22 17 0.4 0.27 10LYM466 62212.3 — — — 6.1 0.21 16 0.4 0.25 9 LYM454 62194.2 — — — — — —0.4 0.27 8 LYM454 62198.4 — — — 6.3 0.11 20 0.4 0.08 14 LYM437 62406.3 —— — — — — 0.4 0.29 8 LYM437 62406.4 — — — 7.2 L 36 0.4 L 22 LYM40762142.2 0.7 0.22 16 — — — — — — LYM407 62142.6 — — — — — — 0.4 0.26 9LYM407 62142.8 — — — — — — 0.4 0.29 9 LYM404 62244.12 0.7 0.28 14 — — —— — — LYM399 62085.2 — — — 6.1 0.19 16 — — — LYM387 62102.4 — — — — — —0.4 0.27 8 LYM373 62159.1 0.8 0.04 26 6.7 0.04 26 0.4 L 21 LYM34362458.4 0.7 0.29 15 — — — — — — LYM329 62417.1 0.7 0.28 14 — — — 0.40.20 11 LYM329 62418.3 — — — — — — 0.4 0.29 8 LYM329 62419.5 — — — 6.40.14 20 0.4 0.11 15 LYM323 62359.3 0.7 0.29 12 — — — — — — LYM32262332.3 — — — 6.0 0.27 14 0.4 0.28 9 LYM322 62336.1 — — — 7.1 L 34 0.40.09 13 LYM321 62264.12 0.8 0.03 27 — — — — — — CONT. — 0.6 — — 5.3 — —0.4 — — LYM493 61966.4 0.9 0.20 17 — — — — — — LYM480 61961.1 0.9 0.2316 — — — — — — LYM458 61812.4 0.9 0.21 16 — — — 0.5 0.18 15 LYM45361985.4 0.9 0.11 21 — — — — — — LYM415 61598.5 — — — 10.7 0.16 22 0.50.27 12 LYM415 61602.6 — — — 10.6 0.21 21 0.5 0.08 24 LYM393 61610.6 — —— 10.5 0.21 20 0.5 0.12 17 LYM380 61824.2 0.9 0.18 18 — — — — — — LYM38061828.3 — — — 10.6 0.18 21 0.5 0.28 12 LYM376 61836.1 — — — 10.7 0.17 210.5 0.15 16 LYM366 61910.6 — — — 10.2 0.29 16 — — — LYM361 61795.2 — — —— — — 0.5 0.13 16 LYM344 61788.4 0.9 0.29 13 — — — — — — LYM327 61846.1— — — 10.3 0.26 17 — — — LYM327 61847.1 0.9 0.28 14 — — — — — — LYM32061854.2 0.9 0.30 14 11.5 0.05 31 0.5 0.15 16 LYM318 61624.1 — — — 10.20.30 17 0.5 0.21 14 CONT. — 0.8 — — 8.8 — — 0.4 — — Table 35.“CONT.”—Control; “Ave.”—Average; “% Incr.” = % increment;“p-val.”—p-value, L—p < 0.01. RGR = relative growth rate. The transgeneswere under the transcriptional regulation of the new At6669 promoter(SEQ ID NO: 4668).

TABLE 36 Genes showing improved plant performance at Normal growthconditions under regulation of At6669 promoter Rosette Rosette DiameterHarvest Index Area [cm²] [cm] Gene P- % P- % P- % Name Event # Ave. Val.Incr. Ave. Val. Incr. Ave. Val. Incr. LYM507 62272.9 — — — 6.4 0.07 194.3 0.19 7 LYM506 62208.4 — — — 5.8 0.13 8 4.2 0.29 5 LYM505 62279.5 — —— 5.8 0.17 9 4.2 0.10 5 LYM498 62078.2 — — — 5.7 0.21 6 — — — LYM49462414.5 — — — 6.9 0.12 28 4.6 0.11 15 LYM492 62136.6 — — — 6.0 0.08 134.3 0.07 8 LYM466 62212.3 — — — 6.1 L 13 4.3 0.03 8 LYM454 62194.2 — — —5.7 0.14 6 4.2 0.02 6 LYM454 62198.4 — — — 6.3 0.19 18 4.4 0.01 10LYM438 62186.3 — — — — — — 4.2 0.13 5 LYM437 62406.3 — — — — — — 4.20.29 4 LYM437 62406.4 — — — 7.3 0.10 36 4.7 L 18 LYM424 62284.11 — — — —— — 4.2 0.12 4 LYM407 62145.1 — — — — — — 4.1 0.24 3 LYM399 62085.2 — —— 6.2 L 16 4.3 0.03 8 LYM387 62102.4 — — — — — — 4.2 0.17 4 LYM37362159.1 — — — 6.6 0.01 24 4.5 L 13 LYM356 62092.3 — — — 5.7 0.12 7 4.30.01 7 LYM329 62418.3 — — — 5.7 0.12 7 4.3 0.02 7 LYM322 62336.1 — — —7.1 0.06 34 4.6 0.01 14 CONT. — — — — 5.3 — — 4.0 — — LYM503 61584.7 0.30.20 17 — — — — — — LYM495 61742.2 0.3 0.20 10 — — — — — — LYM49561744.1 0.3 0.29 15 — — — — — — LYM495 61744.3 0.3 0.04 18 — — — — — —LYM493 61966.4 0.3 0.29 9 — — — — — — LYM493 61968.6 0.3 0.03 20 — — — —— — LYM493 61969.8 0.3 0.26 12 — — — — — — LYM480 61960.6 0.3 0.29 10 —— — — — — LYM473 61783.2 0.3 0.04 20 — — — — — — LYM473 61784.2 0.3 0.2411 — — — — — — LYM473 61784.3 — — — 9.6 0.19 8 5.1 0.16 5 LYM458 61812.4— — — 9.8 0.24 11 5.3 0.13 9 LYM458 61816.4 0.3 0.25 9 — — — — — —LYM456 61588.5 0.3 0.05 16 — — — — — — LYM456 61588.7 — — — 9.8 0.11 105.2 0.10 6 LYM453 61985.4 — — — 10.1 0.09 14 5.1 0.27 5 LYM442 61978.7 —— — 9.6 0.21 8 5.2 0.08 7 LYM442 61979.6 0.3 0.05 18 — — — — — — LYM44061936.6 — — — 9.5 0.21 7 5.2 0.03 8 LYM440 61937.6 — — — 9.8 0.11 10 5.20.09 6 LYM440 61939.6 0.3 0.11 14 — — — — — — LYM415 61598.5 — — — 10.80.04 22 5.4 0.04 12 LYM415 61602.8 0.3 0.11 14 9.4 0.28 6 5.2 0.05 7LYM409 61997.2 0.3 0.19 17 — — — — — — LYM409 61998.6 0.3 0.21 22 — — —5.2 0.09 6 LYM396 61900.7 0.3 0.26 9 — — — — — — LYM393 61610.6 — — —10.6 0.03 19 5.6 0.01 15 LYM393 61610.8 — — — — — — 5.1 0.18 4 LYM38061828.3 — — — 10.7 0.02 21 5.4 L 10 LYM377 61593.5 — — — 9.6 0.30 8 5.20.19 6 LYM377 61594.1 0.3 0.17 13 — — — — — — LYM377 61594.12 0.3 0.0421 — — — — — — LYM377 61594.8 — — — 10.3 0.23 16 5.4 0.25 10 LYM37661836.1 — — — 10.7 L 21 5.4 0.02 11 LYM375 61754.4 0.3 0.29 12 — — — — —— LYM375 61756.1 0.3 0.03 20 — — — — — — LYM372 62002.1 — — — 9.7 0.26 9— — — LYM372 62003.6 0.3 0.14 22 — — — — — — LYM372 62004.2 0.3 0.12 15— — — — — — LYM366 61906.9 0.3 0.01 24 9.9 0.09 11 5.2 0.05 7 LYM36661910.6 — — — 10.3 0.12 16 5.4 0.03 12 LYM361 61794.3 0.3 0.25 9 — — — —— — LYM361 61795.2 — — — 10.2 0.15 15 5.5 L 12 LYM361 61795.4 0.3 0.2611 — — — — — — LYM361 61797.1 — — — — — — 5.2 0.26 7 LYM354 61804.3 — —— 10.0 0.23 13 5.2 0.14 6 LYM346 61616.16 0.3 0.06 22 — — — — — — LYM34661616.9 0.3 0.16 11 — — — — — — LYM344 61788.2 0.3 0.29 8 — — — — — —LYM344 61790.1 — — — 9.8 0.14 10 5.1 0.13 5 LYM344 61790.3 0.3 0.15 13 —— — — — — LYM334 61942.7 0.3 0.07 14 — — — — — — LYM334 61943.12 — — — —— — 5.1 0.23 4 LYM330 61840.1 0.3 0.08 23 — — — — — — LYM330 61840.3 0.30.18 10 — — — — — — LYM330 61842.4 0.3 0.02 21 — — — — — — LYM33061844.3 0.3 0.29 12 — — — — — — LYM327 61846.1 — — — 10.6 0.07 19 5.30.02 9 LYM327 61847.1 0.3 0.19 21 — — — — — — LYM320 61852.4 0.3 0.16 11— — — — — — LYM320 61854.2 — — — 11.5 L 29 5.7 0.03 16 LYM318 61622.20.3 0.21 12 — — — — — — LYM318 61623.2 0.3 0.03 18 — — — — — — LYM31861624.1 0.3 0.26 8 — — — 5.3 0.23 10 LYM313 61629.1 0.3 0.09 15 9.5 0.267 5.1 0.11 5 LYM313 61631.3 0.3 0.24 9 10.2 0.04 15 5.3 0.19 8 LYM31061634.4 0.3 0.20 16 — — — — — — LYM301 61916.2 0.3 0.22 25 — — — — — —LYM300 61750.3 — — — — — — 5.1 0.23 4 LYM300 61750.4 — — — — — — 5.00.29 3 LYM299 61806.4 0.3 0.12 19 — — — — — — LYM299 61807.3 0.3 0.29 12— — — — — — LYM299 61808.4 0.3 0.16 11 — — — — — — LYM299 61809.2 0.30.08 15 — — — — — — CONT. — 0.2 — — 8.9 — — 4.9 — — Table 36.“CONT.”—Control; “Ave.”—Average; “% Incr.” = % increment;“p-val.”—p-value, L—p < 0.01. The transgenes were under thetranscriptional regulation of the new At6669 promoter (SEQ ID NO: 4668).

TABLE 37 Genes showing improved plant performance at Normal growthconditions under regulation of At6669 promoter Gene Seed Yield [mg] 1000Seed Weight [mg] Name Event # Ave. P-Val. % Incr. Ave. P-Val. % Incr.LYM509 62202.1 382.3 0.03 17 — — — LYM506 62206.2 445.2 L 36 — — —LYM466 62212.3 373.1 0.29 14 — — — LYM424 62284.1 413.6 L 26 — — —LYM322 62334.5 437.0 0.26 34 — — — LYM317 62251.1 2365.1 0.19 12 — — —LYM302 62258.1 421.3 L 29 — — — CONT. — 327.1 — — — — — LYM503 61584.7281.9 0.09 16 — — — LYM495 61743.2 262.8 0.30 8 — — — LYM495 61744.1275.1 0.12 13 — — — LYM493 61966.4 263.6 0.26 8 — — — LYM493 61967.6306.8 0.28 26 — — — LYM493 61968.6 267.3 0.24 10 — — — LYM493 61969.8298.8 0.03 23 — — — LYM473 61783.2 286.4 0.04 18 — — — LYM473 61784.2285.5 0.29 17 — — — LYM456 61588.5 279.0 0.24 14 — — — LYM453 61986.6 —— — 28.7 0.01 32 LYM442 61980.5 — — — 23.2 0.26 7 LYM440 61937.6 — — —24.2 0.29 11 LYM415 61598.5 — — — 27.9 0.28 28 LYM413 61819.3 275.0 0.1213 — — — LYM409 61997.2 282.2 0.11 16 — — — LYM396 61902.7 — — — 24.60.05 13 LYM380 61824.2 270.1 0.19 11 — — — LYM380 61828.3 — — — 25.3 L16 LYM377 61593.5 — — — 22.9 0.26 5 LYM377 61594.1 279.0 0.07 14 — — —LYM376 61835.2 — — — 23.8 0.19 10 LYM376 61836.1 — — — 23.4 0.15 8LYM361 61794.3 — — — 25.0 0.21 15 LYM354 61804.3 — — — 25.6 0.27 18LYM344 61788.4 — — — 26.5 L 22 LYM334 61942.7 284.5 0.04 17 — — — LYM30161913.4 — — — 23.6 0.07 9 LYM300 61750.4 266.8 0.21 10 — — — CONT. —243.7 — — 21.8 — — Table 37. “CONT.”—Control; “Ave.”—Average; “% Incr.”= % increment; “p-val.”—p-value, L—p < 0.01. The transgenes were underthe transcriptional regulation of the new At6669 promoter (SEQ ID NO:4668).

Assay 2: Plant Performance Improvement Measured Until Bolting Stage:Plant Biomass and Plant Growth Rate Under Normal Greenhouse Conditions(GH-SB Assays)

This assay follows the plant biomass formation and the rosette areagrowth of plants grown in the greenhouse under normal growth conditions.Transgenic Arabidopsis seeds were sown in agar media supplemented with ½MS medium and a selection agent (Kanamycin). The T₂ transgenic seedlingswere then transplanted to 1.7 trays filled with peat and perlite in a1:1 ratio. The trays were irrigated with a solution containing of 6 mMinorganic nitrogen in the form of KNO₃ with 1 mM KH₂PO₄, 1 mM MgSO₄, 2mM CaCl₂ and microelements. All plants were grown in the greenhouseuntil mature seeds. Plant biomass (the above ground tissue) was weightin directly after harvesting the rosette (plant fresh weight [FW]).Following plants were dried in an oven at 50° C. for 48 hours andweighted (plant dry weight [DW]).

Each construct was validated at its T₂ generation. Transgenic plantstransformed with a construct conformed by an empty vector carrying the35S promoter and the selectable marker was used as control.

The plants were analyzed for their overall size, growth rate, freshweight and dry matter. Transgenic plants performance was compared tocontrol plants grown in parallel under the same conditions.Mock-transgenic plants expressing the uidA reporter gene (GUS-Intron) orwith no gene at all, under the same promoter were used as control.

The experiment was planned in nested randomized plot distribution. Foreach gene of the invention three to five independent transformationevents were analyzed from each construct.

Digital Imaging—

A laboratory image acquisition system, which consists of a digitalreflex camera (Canon EOS 300D) attached with a 55 mm focal length lens(Canon EF-S series), mounted on a reproduction device (Kaiser RS), whichincludes 4 light units (4×150 Watts light bulb) was used for capturingimages of plant samples.

The image capturing process was repeated every 2 days starting from day1 after transplanting till day 15. Same camera, placed in a custom madeiron mount, was used for capturing images of larger plants sawn in whitetubs in an environmental controlled greenhouse. The tubs were squareshape include 1.7 liter trays. During the capture process, the tubeswere placed beneath the iron mount, while avoiding direct sun light andcasting of shadows.

An image analysis system was used, which consists of a personal desktopcomputer (Intel P4 3.0 GHz processor) and a public domain program—ImageJ1.39 [Java based image processing program which was developed at theU.S. National Institutes of Health and freely available on the internetat Hypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/]. Imageswere captured in resolution of 10 Mega Pixels (3888×2592 pixels) andstored in a low compression JPEG (Joint Photographic Experts Groupstandard) format. Next, analyzed data was saved to text files andprocessed using the JMP statistical analysis software (SAS institute).

Leaf Analysis—

Using the digital analysis leaves data was calculated, including leafnumber, rosette area, rosette diameter, and leaf blade area.

Vegetative growth rate: the relative growth rate (RGR) of leaf number(Formula X, described above), rosette area (Formula XII described above)and plot coverage (Formula XIII, described above) were calculated usingthe indicated formulas.

Plant Fresh and Dry Weight—

On about day 80 from sowing, the plants were harvested and directlyweight for the determination of the plant fresh weight (FW) and left todry at 50° C. in a drying chamber for about 48 hours before weighting todetermine plant dry weight (DW).

Statistical Analyses—

To identify outperforming genes and constructs, results from theindependent transformation events tested were analyzed separately. Datawas analyzed using Student's t-test and results are consideredsignificant if the p value was less than 0.1. The JMP statisticssoftware package was used (Version 5.2.1, SAS Institute Inc., Cary,N.C., USA).

Experimental Results:

The genes listed in Tables 38-42 improved plant performance when grownat normal conditions. These genes produced larger plants with a largerphotosynthetic area, biomass (fresh weight, dry weight, rosettediameter, rosette area and plot coverage). The genes were cloned underthe regulation of a constitutive (At6669; SEQ ID NO:4668). Theevaluation of each gene was performed by testing the performance ofdifferent number of events. Event with p-value <0.1 was consideredstatistically significant

Tables 38-42 summarize the observed phenotypes of transgenic plantsexpressing the genes constructs using the GH-SB Assays.

TABLE 38 Genes showing improved plant performance at Normal growthconditions under regulation of At6669 promoter Dry Weight [mg] FreshWeight [mg] Leaf Number Gene P- % P- % P- % Name Event # Ave. Val. Incr.Ave. Val. Incr. Ave. Val. Incr. LYM502 62984.2 — — — 3031.2 0.30 11 — —— LYM502 62984.3 — — — — — — 10.6 0.28 7 LYM491 62814.1 — — — 3112.5 L14 — — — LYM490 62918.4 232.5 0.23 15 — — — — — — LYM490 62920.3 — — —3187.5 0.25 16 — — — LYM490 62922.3 223.1 0.24 10 2906.2 0.09 6 — — —LYM484 63361.4 217.5 L 7 3118.8 0.02 14 10.2 0.23 3 LYM484 63362.3 233.80.16 15 — — — — — — LYM448 62802.2 — — — — — — 10.2 0.27 2 LYM44862802.6 — — — 2943.8 0.12 7 10.8 0.11 8 LYM445 62796.4 212.5 0.08 52937.5 0.15 7 — — — LYM445 62797.2 230.0 0.11 13 — — — 10.9 L 10 LYM44562797.3 — — — 2856.2 0.23 4 — — — LYM436 62813.4 225.0 0.24 11 2931.20.14 7 — — — LYM435 62872.1 — — — 3106.2 0.30 13 — — — LYM435 62875.1216.2 0.28 7 — — — 10.6 0.02 6 LYM432 62946.1 208.1 0.21 3 — — — 11.10.02 11 LYM428 63411.2 — — — 3062.5 0.03 12 11.6 0.06 16 LYM419 62774.5— — — 2975.0 0.10 9 — — — LYM419 62778.2 223.8 0.11 10 3275.0 L 19 — — —LYM414 62968.1 — — — 3062.5 0.10 12 10.6 0.28 7 LYM414 62969.4 — — —3237.5 0.02 18 — — — LYM410 62963.2 218.8 0.23 8 — — — — — — LYM40162853.1 — — — 2925.0 0.07 7 — — — LYM401 62856.1 — — — 2843.8 0.27 4 — —— LYM398 62886.2 — — — — — — 10.8 0.01 8 LYM395 62924.2 222.5 L 10 — — —— — — LYM395 62927.1 210.0 0.11 4 — — — — — — LYM395 62928.1 222.5 0.1810 3143.8 L 15 10.6 0.02 6 LYM394 62912.1 — — — 2968.8 0.09 8 — — —LYM394 62912.2 — — — 2925.0 0.07 7 — — — LYM394 62913.2 222.5 0.27 10 —— — 10.4 0.29 4 LYM389 62900.2 221.9 L 9 3131.2 L 14 — — — LYM37162847.2 216.2 0.01 7 — — — — — — LYM371 62847.3 216.9 0.10 7 2918.8 0.076 — — — LYM365 62718.5 — — — 3012.5 0.10 10 — — — LYM365 62721.2 212.50.24 5 — — — 10.5 0.20 5 LYM352 62876.1 214.4 0.30 6 — — — — — — LYM35262876.4 208.1 0.21 3 — — — — — — LYM349 63556.3 219.4 0.25 8 — — — — — —LYM349 63557.2 — — — 2971.4 0.03 8 — — — LYM349 63557.3 — — — 3106.20.28 13 — — — LYM338 62949.6 220.0 0.04 9 3087.5 0.04 13 — — — LYM33562895.1 — — — 3012.5 0.02 10 — — — LYM335 62896.2 — — — 2968.8 0.25 810.2 0.16 3 LYM333 62888.12 — — — 2857.1 0.30 4 — — — LYM333 62890.1233.8 0.09 15 2856.2 0.26 4 — — — LYM331 62932.2 221.2 0.03 9 — — — — —— LYM331 62934.3 223.1 0.01 10 — — — — — — LYM328 62728.1 — — — 3037.50.08 11 — — — LYM328 62732.6 216.9 0.01 7 — — — — — — LYM324 62973.6216.9 0.10 7 3200.0 0.16 17 — — — LYM324 62975.3 — — — 3118.8 0.11 14 —— — LYM316 63367.1 216.2 0.01 7 — — — — — — LYM314 62859.1 209.2 0.18 3— — — — — — LYM314 62861.3 — — — 2931.2 0.14 7 — — — LYM314 62862.1215.6 0.01 6 3225.0 0.15 18 10.4 0.04 5 LYM311 62954.5 228.1 0.18 13 — —— — — — LYM311 62956.1 218.1 L 8 — — — — — — LYM311 62956.2 219.4 L 82900.0 0.12 6 — — — LYM308 63288.5 219.4 0.19 8 3075.0 L 12 — — — LYM30863290.2 216.2 0.14 7 — — — — — — LYM298 62722.1 — — — — — — 10.3 0.26 4LYM345 62936.2 208.1 0.33 3 2950 0.42 8 — — — LYM345 62936.2 — — —2812.5 0.42 3 — — — LYM457 63206.2 — — — 2968.8 0.43 8 — — — CONT. —202.7 — — 2741.1 — — 10.0 — — LYM510 62475.1 256.2 0.08 21 — — — — — —LYM510 62478.4 — — — 3025.0 L 7 10.6 0.09 7 LYM501 62537.1 271.9 0.04 293468.8 L 23 — — — LYM488 62564.7 233.8 0.24 11 3131.2 0.18 11 — — —LYM488 62567.3 232.5 0.04 10 — — — — — — LYM481 62463.2 — — — 3050.00.25 8 — — — LYM481 62466.3 236.9 0.14 12 3100.0 0.02 10 — — — LYM47162657.1 — — — — — — 10.2 0.07 3 LYM471 62658.1 225.6 0.10 7 3018.8 0.017 — — — LYM471 62658.2 248.8 0.03 18 3206.2 L 14 10.7 L 8 LYM471 62658.4— — — 2950.0 0.07 5 — — — LYM465 62338.2 233.1 0.27 10 — — — — — —LYM465 62340.2 245.0 L 16 3125.0 0.01 11 — — — LYM460 62423.3 — — — — —— 10.4 0.26 5 LYM460 62425.1 236.2 0.06 12 — — — — — — LYM455 62704.1223.1 0.17 6 2981.2 0.08 6 — — — LYM455 62705.5 — — — 3443.8 0.09 2211.2 L 14 LYM455 62708.5 — — — — — — 10.1 0.27 2 LYM451 62486.6 233.10.02 10 3062.5 0.23 9 — — — LYM451 62488.3 245.6 0.20 16 — — — — — —LYM451 62488.5 223.1 0.26 6 2900.0 0.29 3 — — — LYM447 62683.1 220.00.29 4 — — — 10.1 0.27 2 LYM447 62683.2 — — — — — — 10.2 0.12 4 LYM44662492.1 — — — — — — 10.1 0.26 2 LYM446 62495.1 240.0 0.10 14 3231.2 L 1510.2 0.12 4 LYM444 62626.1 283.1 0.29 34 3181.2 L 13 — — — LYM44462627.3 — — — 3056.2 0.26 8 — — — LYM444 62628.4 223.1 0.16 6 3043.8 L 8— — — LYM416 62663.1 — — — 3106.2 0.17 10 — — — LYM416 62663.3 225.60.10 7 — — — — — — LYM416 62664.2 — — — 2993.8 0.16 6 — — — LYM40662562.1 223.8 0.18 6 3106.2 L 10 10.3 0.20 4 LYM406 62562.2 244.4 0.2116 3106.2 L 10 10.1 0.26 2 LYM390 62472.1 231.2 0.19 9 2900.0 0.24 3 — —— LYM388 62540.1 362.5 0.16 72 3525.0 0.15 25 10.1 0.26 2 LYM378 62698.1— — — 2906.2 0.19 3 — — — LYM374 62505.2 — — — — — — 10.8 0.05 9 LYM36962548.4 — — — 3056.2 0.18 8 — — — LYM369 62548.5 253.7 L 20 — — — — — —LYM369 62550.1 — — — 3000.0 0.18 6 — — — LYM362 62344.6 — — — 2981.20.03 6 10.1 0.27 2 LYM362 62347.2 — — — — — — 10.1 0.26 2 LYM362 62349.4246.2 0.18 17 3137.5 0.16 11 — — — LYM359 62326.1 257.5 L 22 3225.0 L 14— — — LYM359 62326.4 — — — 3006.2 0.09 7 10.1 0.27 2 LYM359 62329.1227.5 0.07 8 3037.5 L 8 — — — LYM355 62450.4 — — — 2943.8 0.08 4 — — —LYM355 62450.6 251.9 0.03 19 3225.0 L 14 — — — LYM355 62451.2 228.8 0.238 — — — — — — LYM355 62451.3 — — — — — — 10.1 0.27 2 LYM355 62451.4 — —— 2925.0 0.13 4 — — — LYM353 62675.4 — — — 3025.0 0.09 7 — — — LYM35362677.1 330.6 0.14 56 — — — — — — LYM353 62677.5 — — — 3325.0 0.04 18 —— — LYM353 62677.6 223.1 0.19 6 2931.2 0.10 4 10.3 0.02 4 LYM351 62529.2233.1 0.06 10 3262.5 L 16 — — — LYM351 62530.3 243.1 L 15 3137.5 L 1111.1 0.07 12 LYM341 62480.2 — — — 2887.5 0.29 2 — — — LYM341 62484.1 — —— 3075.0 0.14 9 11.1 0.07 12 LYM340 62652.1 — — — 3031.2 L 7 — — —LYM340 62653.4 231.9 0.20 10 3031.2 0.25 7 10.1 0.11 2 LYM339 62671.2 —— — 2993.8 0.27 6 — — — LYM339 62671.3 265.0 0.02 25 3162.5 0.12 12 — —— LYM332 62554.3 — — — 2993.8 0.02 6 10.5 L 6 LYM332 62554.7 — — — — — —10.1 0.27 2 LYM332 62555.2 228.1 0.06 8 2956.2 0.18 5 — — — LYM33262556.2 — — — 2900.0 0.29 3 — — — LYM325 62689.2 260.6 0.20 23 3037.50.02 8 — — — LYM325 62689.3 228.8 0.28 8 — — — — — — LYM325 62690.1 — —— — — — 10.1 0.11 2 LYM325 62690.6 — — — 3156.2 0.30 12 10.2 0.07 3LYM306 62433.1 240.0 0.17 14 3137.5 0.19 11 10.1 0.27 2 LYM305 62518.1228.1 0.13 8 2993.8 0.03 6 — — — LYM305 62519.4 — — — 2975.0 0.06 5 — —— LYM303 62523.1 — — — — — — 10.2 0.07 3 LYM303 62525.3 — — — — — — 10.10.11 2 LYM303 62526.4 226.9 0.08 7 — — — — — — LYM441 62361.2 223.7 0.416 293 0.48 4 — — — CONT. — 211.4 — — 2821.7 — — 9.9 — — LYM503 61581.6 —— — — — — 11.9 0.03 5 LYM503 61584.1 — — — — — — 12.0 0.02 6 LYM50361584.7 — — — — — — 11.9 L 6 LYM495 61744.1 331.9 0.24 7 3200.0 0.25 9 —— — LYM493 61969.12 — — — — — — 11.6 0.14 3 LYM480 61962.8 — — — — — —12.1 L 7 LYM474 61977.6 — — — 3543.8 0.20 21 — — — LYM473 61783.2 369.40.14 19 3575.0 0.07 22 — — — LYM473 61783.4 — — — 3462.5 0.06 18 — — —LYM456 61587.8 — — — — — — 11.7 0.03 4 LYM456 61589.4 356.2 0.12 153762.5 L 29 — — — LYM456 61590.8 350.0 0.07 13 3756.2 0.02 28 11.9 0.035 LYM453 61984.7 — — — 3218.8 0.26 10 — — — LYM453 61984.9 — — — 3450.00.05 18 — — — LYM453 61986.6 404.4 0.27 31 4112.5 0.14 41 — — — LYM45361988.6 333.1 0.28 8 3700.0 0.05 26 — — — LYM442 61978.7 — — — 3216.10.24 10 — — — LYM442 61979.6 — — — 3583.9 0.04 22 — — — LYM442 61980.5331.9 0.23 7 3512.5 0.04 20 — — — LYM442 61983.7 346.4 0.07 12 3371.40.10 15 — — — LYM440 61936.6 350.6 0.19 13 3750.0 0.08 28 — — — LYM44061937.6 — — — 3556.2 0.04 22 — — — LYM440 61937.8 — — — 3425.0 0.06 17 —— — LYM440 61939.6 — — — 3337.5 0.11 14 — — — LYM415 61598.5 — — — — — —12.1 0.24 7 LYM415 61598.7 — — — 3318.8 0.15 13 — — — LYM415 61600.5361.9 0.02 17 3850.0 0.01 32 — — — LYM415 61602.6 — — — 3462.5 0.19 1811.8 L 5 LYM415 61602.8 — — — 3318.8 0.25 13 — — — LYM413 61821.1 3281.20.25 12 — — — LYM413 61823.1 348.4 0.06 12 3723.2 L 27 — — — LYM40961998.2 406.2 L 31 4082.1 L 39 — — — LYM409 61998.6 403.8 L 30 4303.6 L47 — — — LYM409 61999.2 353.1 0.08 14 3650.0 0.05 25 — — — LYM40961999.3 380.0 L 23 3931.2 0.01 34 11.7 0.03 4 LYM409 61999.5 370.0 L 193706.2 L 27 — — — LYM396 61900.1 380.6 L 23 3943.8 0.05 35 — — — LYM39661901.1 341.2 0.11 10 — — — — — — LYM396 61902.7 348.7 0.05 13 3465.20.17 18 — — — LYM393 61610.6 413.1 0.13 33 4218.8 L 44 — — — LYM39361610.8 391.2 0.25 26 4031.2 L 38 — — — LYM393 61614.6 — — — 3925.0 0.1634 — — — LYM393 61614.9 366.9 0.02 18 3825.0 0.04 31 — — — LYM38061824.2 404.9 0.16 31 4128.6 0.03 41 11.8 0.07 4 LYM380 61825.4 — — —3693.8 0.17 26 — — — LYM380 61825.5 378.1 L 22 3906.2 L 33 — — — LYM38061828.3 431.9 0.14 39 4231.2 0.06 45 11.9 0.03 5 LYM380 61828.5 388.1 L25 4156.2 L 42 — — — LYM377 61592.5 419.4 L 35 4375.0 L 49 12.0 0.02 6LYM377 61594.1 400.0 L 29 4156.2 L 42 11.6 0.11 2 LYM377 61594.12 355.60.04 15 3531.2 0.10 21 — — — LYM377 61594.8 386.2 0.03 25 3931.2 L 34 —— — LYM376 61835.2 393.1 L 27 4250.0 L 45 — — — LYM376 61835.3 — — — — —— 11.5 0.17 2 LYM376 61836.1 359.4 0.21 16 3468.8 0.23 19 — — — LYM37661837.1 366.9 0.01 18 3768.8 0.03 29 — — — LYM375 61754.4 351.2 0.04 133312.5 0.21 13 — — — LYM375 61756.1 403.8 0.03 30 4331.2 0.02 48 12.3 L9 LYM375 61758.1 353.8 0.04 14 3468.8 0.04 19 — — — LYM372 62003.2 — — —3375.0 0.08 15 — — — LYM372 62003.6 354.4 0.04 14 3431.2 0.08 17 — — —LYM366 61906.15 379.4 L 22 4006.2 0.07 37 — — — LYM366 61906.9 — — —3737.5 0.24 28 11.6 0.04 3 LYM366 61910.6 407.5 0.02 32 4218.8 0.02 44 —— — LYM366 61910.7 — — — 3462.5 0.19 18 — — — LYM361 61795.1 429.4 0.2039 4168.8 0.19 42 — — — LYM361 61795.2 378.1 L 22 3862.5 L 32 — — —LYM361 61796.4 386.8 L 25 3993.8 L 36 — — — LYM361 61797.1 361.9 0.02 173543.8 0.03 21 — — — LYM354 61800.4 390.6 0.22 26 3987.5 L 36 11.9 L 6LYM354 61801.3 407.5 L 32 4187.5 L 43 — — — LYM354 61803.4 370.0 0.02 193837.5 0.11 31 — — — LYM354 61804.3 400.0 0.07 29 4037.5 L 38 — — —LYM354 61804.4 404.4 0.01 31 4181.2 0.10 43 — — — LYM346 61616.15 373.30.10 20 3792.9 0.04 30 — — — LYM346 61616.16 383.1 L 24 3987.5 0.06 36 —— — LYM346 61617.9 418.8 0.01 35 4350.0 L 49 — — — LYM346 61618.4 371.20.11 20 3693.8 L 26 — — — LYM344 61788.2 359.9 0.18 16 3638.4 0.02 24 —— — LYM344 61788.4 370.0 0.24 19 3727.7 0.16 27 11.6 0.14 3 LYM34461790.1 413.8 L 34 4031.2 L 38 — — — LYM344 61790.3 395.0 0.02 27 3943.80.07 35 11.9 0.25 6 LYM344 61791.1 355.6 0.03 15 3993.8 0.02 36 — — —LYM334 61942.6 358.1 0.16 16 3762.5 0.02 29 — — — LYM334 61942.7 — — —3550.0 0.02 21 — — — LYM334 61942.8 387.5 0.01 25 3868.8 L 32 — — —LYM334 61943.12 361.2 0.15 17 3950.0 L 35 — — — LYM334 61947.7 391.90.24 26 3841.2 0.02 31 — — — LYM330 61840.1 383.1 0.26 24 4013.4 0.09 3711.8 0.27 4 LYM330 61841.4 — — — 3487.5 0.27 19 — — — LYM330 61842.4374.7 L 21 3872.3 0.21 32 — — — LYM330 61844.3 362.1 0.04 17 3818.8 0.0330 — — — LYM327 61846.1 384.4 L 24 4000.0 0.04 37 — — — LYM327 61846.3341.2 0.23 10 — — — — — — LYM327 61847.1 366.3 0.01 18 3586.6 0.09 23 —— — LYM327 61849.1 — — — 3471.4 0.04 19 — — — LYM320 61851.2 — — —3700.0 0.16 26 — — — LYM320 61852.4 371.2 L 20 3956.2 L 35 — — — LYM32061853.2 341.2 0.14 10 3431.2 0.07 17 — — — LYM320 61854.2 352.5 0.17 143693.8 0.10 26 — — — LYM319 61918.5 393.1 0.05 27 4206.2 L 44 11.6 0.112 LYM319 61920.6 — — — 3587.5 0.19 23 — — — LYM319 61920.7 362.5 0.06 174018.8 L 37 — — — LYM319 61921.5 380.0 0.01 23 3825.0 L 31 — — — LYM31961921.7 353.1 0.07 14 3762.5 0.02 29 — — — LYM318 61623.3 354.0 0.06 143569.6 0.02 22 — — — LYM318 61625.4 — — — 3642.9 0.24 24 — — — LYM31861625.6 377.2 0.02 22 3817.9 0.15 30 — — — LYM313 61628.2 386.0 L 254128.6 L 41 — — — LYM313 61629.1 — — — 3668.8 0.26 25 12.0 0.28 6 LYM31361630.1 — — — 3437.5 0.08 17 — — — LYM313 61630.2 383.1 0.05 24 4025.0 L38 11.6 0.11 2 LYM313 61631.3 372.1 L 20 3779.2 L 29 — — — LYM31061634.4 333.8 0.24 8 3337.5 0.17 14 — — — LYM310 61636.2 343.3 0.08 113573.2 0.02 22 — — — LYM310 61637.2 357.5 0.05 15 3712.5 0.01 27 — — —LYM310 61637.4 388.1 0.01 25 4112.5 L 41 — — — LYM310 61638.4 398.8 L 294206.2 L 44 — — — LYM301 61912.3 357.5 0.02 15 3743.8 0.02 28 — — —LYM301 61913.3 375.0 0.02 21 3793.8 L 30 11.6 0.14 3 LYM301 61914.1377.6 0.14 22 3808.9 0.07 30 11.6 0.14 3 LYM301 61914.2 384.4 0.01 243856.2 0.01 32 — — — LYM301 61916.2 381.0 L 23 4017.9 L 37 — — — LYM30061748.4 331.0 0.28 7 3487.5 0.12 19 — — — LYM300 61749.4 360.6 0.24 163606.2 0.12 23 — — — LYM300 61750.3 373.8 0.07 21 3931.2 L 34 — — —LYM300 61750.4 377.5 0.15 22 3950.0 L 35 — — — LYM300 61752.3 383.1 L 243900.0 L 33 11.6 0.14 3 LYM299 61806.2 368.1 L 19 3756.2 0.03 28 — — —LYM299 61806.4 435.6 L 41 4587.5 L 57 — — — LYM299 61807.4 399.4 0.04 294000.0 0.20 37 — — — LYM299 61808.4 386.9 L 25 4075.0 L 39 — — — LYM29961809.2 367.5 0.01 19 3937.5 L 35 — — — CONT. — 309.8 — — 2926.6 — —11.3 — — LYM509 62203.4 370.4 0.20 18 — — — — — — LYM507 62273.12 — — —3343.8 0.12 11 — — — LYM500 62367.4 — — — — — — 9.6 0.10 3 LYM49962096.1 — — — 3250.0 0.06 8 — — — LYM499 62096.2 — — — — — — 9.9 0.21 6LYM498 62078.6 — — — — — — 9.6 0.10 3 LYM498 62079.2 380.6 0.12 213500.0 L 17 — — — LYM494 62411.4 — — — 3500.0 0.29 17 — — — LYM49462414.5 — — — 3537.5 0.09 18 — — — LYM492 62136.6 409.4 0.05 31 4506.20.03 50 9.9 0.21 6 LYM492 62137.4 — — — — — — 9.6 0.20 3 LYM492 62141.1379.4 0.08 21 4093.8 L 36 — — — LYM489 61833.1 337.5 0.26 8 — — — — — —LYM489 61834.1 362.2 0.09 16 4037.5 L 34 — — — LYM487 62151.3 — — — — —— 9.6 0.20 3 LYM477 62052.5 390.4 0.19 25 4600.0 0.09 53 — — — LYM47762053.4 388.8 L 24 4181.2 0.02 39 — — — LYM464 62064.2 408.6 L 30 4457.1L 48 — — — LYM461 62219.8 369.6 0.19 18 3172.6 0.20 6 — — — LYM45462194.2 394.4 0.05 26 4243.8 L 41 — — — LYM454 62196.4 348.1 0.14 113268.8 0.23 9 — — — LYM437 62406.3 383.8 0.18 22 4250.0 L 42 9.9 0.04 6LYM424 62285.12 438.3 0.19 40 4854.2 0.04 62 — — — LYM424 62288.9 — — —3350.0 0.01 12 — — — LYM418 62227.2 — — — 3518.8 0.03 17 — — — LYM41862228.4 — — — 3781.2 0.24 26 — — — LYM407 62142.2 — — — 3568.8 0.17 199.8 0.02 5 LYM407 62142.6 392.6 0.25 25 3489.3 0.25 16 — — — LYM40762145.1 409.4 0.11 31 4325.0 L 44 — — — LYM407 62145.2 388.8 0.21 244206.2 L 40 — — — LYM404 62244.12 — — — 3156.2 0.24 5 — — — LYM39962086.3 — — — 3906.2 L 30 — — — LYM382 62059.2 359.4 0.29 15 4243.8 L 419.8 0.09 5 LYM382 62061.2 371.5 0.21 18 3482.1 0.08 16 — — — LYM36362071.1 425.4 0.15 36 — — — — — — LYM323 62356.5 — — — — — — 9.6 0.10 3LYM323 62358.4 413.8 0.06 32 3844.6 L 28 — — — LYM322 62334.5 — — — — —— 9.8 0.15 5 LYM322 62336.1 — — — — — — 9.7 0.07 4 LYM321 62264.12 400.00.20 28 3625.0 L 21 9.9 0.04 6 LYM317 62251.12 — — — — — — 9.8 0.15 5LYM302 62257.11 384.4 0.22 23 4131.2 0.10 38 9.8 0.02 5 CONT. — 313.5 —— 3003.4 — — 9.3 — — Table 38. “CONT.”—Control; “Ave.”—Average; “%Incr.” = % increment; “p-val.”—p-value, L—p < 0.01. The transgenes wereunder the transcriptional regulation of the new At6669 promoter (SEQ IDNO: 4668).

TABLE 39 Genes showing improved plant performance at Normal growthconditions under regulation of At6669 promoter Rosette Diameter PlotCoverage [cm²] Rosette Area [cm²] [cm] Gene P- % P- % P- % Name Event #Ave. Val. Incr. Ave. Val. Incr. Ave. Val. Incr. LYM490 62918.4 — — — — —— 4.7 0.10 5 LYM490 62922.3 61.1 0.09 11 7.6 0.09 11 4.8 0.02 8 LYM48463361.4 60.2 0.14 9 7.5 0.14 9 4.6 0.20 4 LYM445 62796.4 64.2 0.04 178.0 0.04 17 4.8 0.02 8 LYM435 62872.1 59.7 0.23 8 7.5 0.23 8 4.7 0.14 5LYM435 62875.1 67.7 0.24 23 8.5 0.24 23 4.9 0.21 9 LYM428 63411.2 71.70.05 30 9.0 0.05 30 5.2 0.03 16 LYM419 62775.1 60.9 0.22 11 7.6 0.22 114.7 0.06 6 LYM419 62778.2 — — — — — — 4.8 0.05 8 LYM414 62968.1 64.60.02 17 8.1 0.02 17 4.8 0.04 9 LYM410 62963.2 — — — — — — 4.6 0.25 3LYM395 62928.1 61.1 0.29 11 7.6 0.29 11 — — — LYM394 62913.2 59.4 0.20 87.4 0.20 8 4.6 0.19 4 LYM365 62721.2 — — — — — — 4.6 0.27 4 LYM33362890.1 62.6 0.26 14 7.8 0.26 14 4.8 0.07 8 LYM324 62975.3 62.8 0.20 147.9 0.20 14 4.7 0.25 6 LYM308 63288.5 62.2 0.29 13 7.8 0.29 13 4.8 0.248 CONT. — 55.1 — — 6.9 — — 4.5 — — LYM510 62475.1 55.9 L 15 7.0 L 15 4.7L 9 LYM510 62478.3 52.6 0.10 9 6.6 0.10 9 4.4 0.13 4 LYM510 62478.4 51.40.17 6 6.4 0.17 6 4.4 0.20 3 LYM501 62535.2 54.4 0.27 12 6.8 0.27 12 4.70.19 9 LYM501 62537.1 62.4 L 29 7.8 L 29 5.1 L 18 LYM488 62564.6 52.40.19 8 6.5 0.19 8 4.5 0.24 5 LYM488 62566.1 50.4 0.18 4 6.3 0.18 4 4.40.18 3 LYM486 62371.6 — — — — — — 4.4 0.26 3 LYM481 62463.2 53.2 0.03 106.7 0.03 10 — — — LYM481 62466.3 53.2 0.06 10 6.6 0.06 10 4.6 0.11 7LYM471 62658.1 54.3 0.01 12 6.8 0.01 12 4.6 0.05 7 LYM471 62658.2 61.80.13 27 7.7 0.13 27 5.0 L 15 LYM471 62659.5 — — — — — — 4.5 0.23 5LYM465 62340.2 53.3 L 10 6.7 L 10 4.6 0.02 7 LYM465 62342.4 58.7 0.23 217.3 0.23 21 4.6 0.09 7 LYM460 62425.1 — — — — — — 4.7 0.24 9 LYM45562705.5 65.2 L 34 8.2 L 34 4.9 0.02 14 LYM451 62486.2 51.2 0.20 5 6.40.20 5 4.4 0.29 2 LYM451 62488.3 59.2 L 22 7.4 L 22 4.8 L 12 LYM45162488.5 52.8 0.07 9 6.6 0.07 9 4.5 0.08 4 LYM446 62495.1 58.6 0.01 217.3 0.01 21 4.8 L 12 LYM444 62626.1 — — — — — — 4.5 0.06 5 LYM44462628.4 54.5 0.16 12 6.8 0.16 12 4.7 L 9 LYM416 62663.1 55.2 L 14 6.9 L14 4.7 0.02 9 LYM406 62562.1 — — — — — — 4.5 0.08 4 LYM406 62562.2 54.90.08 13 6.9 0.08 13 4.6 0.02 6 LYM388 62540.3 — — — — — — 4.5 0.09 4LYM388 62543.1 — — — — — — 4.6 0.09 6 LYM369 62548.4 — — — — — — 4.50.28 4 LYM369 62550.1 51.0 0.08 5 6.4 0.08 5 4.6 0.01 7 LYM364 62694.156.9 0.25 17 7.1 0.25 17 — — — LYM362 62344.6 — — — — — — 4.6 0.02 7LYM362 62349.4 57.9 0.22 20 7.2 0.22 20 4.7 0.21 9 LYM359 62329.1 60.70.02 25 7.6 0.02 25 4.9 L 15 LYM355 62450.6 57.5 0.13 19 7.2 0.13 19 4.8L 12 LYM355 62451.2 52.3 0.30 8 6.5 0.30 8 4.5 0.27 5 LYM355 62451.354.0 0.25 11 6.7 0.25 11 4.5 0.27 6 LYM355 62451.4 54.5 0.14 12 6.8 0.1412 4.5 0.08 5 LYM353 62675.4 58.4 L 20 7.3 L 20 4.8 0.03 11 LYM35362677.5 50.9 0.10 5 6.4 0.10 5 4.5 0.10 6 LYM353 62677.6 53.9 0.13 116.7 0.13 11 4.5 0.06 5 LYM351 62529.2 61.7 L 27 7.7 L 27 4.9 L 14 LYM35162530.3 64.9 L 34 8.1 L 34 4.9 L 14 LYM341 62484.1 60.9 0.06 26 7.6 0.0626 4.8 L 11 LYM340 62653.4 52.7 0.05 9 6.6 0.05 9 4.5 0.15 4 LYM33962671.2 57.1 0.11 18 7.1 0.11 18 4.7 L 9 LYM339 62671.3 56.1 L 16 7.0 L16 4.6 0.10 7 LYM332 62554.3 62.2 L 28 7.8 L 28 4.8 L 13 LYM325 62689.252.5 0.22 8 6.6 0.22 8 4.5 0.04 5 LYM325 62690.1 52.7 0.03 9 6.6 0.03 9— — — LYM306 62433.1 58.5 L 21 7.3 L 21 4.8 L 12 LYM305 62518.1 57.10.04 18 7.1 0.04 18 4.6 L 8 LYM305 62519.4 62.7 0.09 29 7.8 0.09 29 4.8L 13 LYM303 62523.1 56.1 0.17 16 7.0 0.17 16 4.6 0.01 8 CONT. — 48.5 — —6.1 — — 4.3 — — LYM503 61584.7 — — — — — — 4.7 0.20 9 LYM495 61742.266.1 0.09 10 8.3 0.13 8 4.6 0.05 6 LYM495 61744.1 73.7 0.24 23 9.2 0.2620 4.9 0.21 13 LYM493 61969.12 73.4 0.24 22 9.2 0.27 20 4.9 0.26 14LYM480 61961.12 — — — — — — 4.5 0.06 4 LYM480 61962.7 — — — — — — 4.50.02 5 LYM474 61975.8 — — — — — — 4.5 0.03 4 LYM474 61976.8 — — — — — —4.7 L 8 LYM473 61783.2 — — — — — — 4.9 0.22 14 LYM458 61816.4 66.5 0.1110 8.3 0.16 9 4.7 0.03 9 LYM456 61587.8 65.5 0.03 9 8.2 0.06 7 4.6 0.046 LYM456 61588.5 — — — — — — 4.7 0.25 9 LYM456 61588.7 74.9 0.29 24 — —— — — — LYM456 61590.8 66.4 0.01 10 8.3 0.02 8 4.7 L 9 LYM453 61986.690.5 0.04 50 11.3 0.04 48 5.6 0.10 30 LYM453 61988.6 81.4 L 35 10.2 L 335.2 L 20 LYM442 61980.5 — — — — — — 4.8 0.26 11 LYM440 61936.6 — — — — —— 5.0 0.21 15 LYM440 61937.6 73.8 0.15 23 9.2 0.17 21 4.9 0.03 14 LYM44061937.8 63.1 0.15 5 — — — 4.6 0.01 7 LYM415 61598.5 73.0 L 21 9.1 L 194.8 0.08 11 LYM415 61600.5 — — — — — — 4.9 0.29 14 LYM415 61602.6 69.50.20 15 8.7 0.23 14 4.9 L 13 LYM409 61998.2 — — — — — — 5.3 0.30 22LYM409 61998.6 76.0 0.18 26 9.5 0.20 24 5.1 0.15 19 LYM409 61999.2 78.70.15 31 9.8 0.16 29 5.1 0.18 18 LYM409 61999.3 74.8 0.28 24 — — — 5.00.24 15 LYM409 61999.5 68.9 L 15 8.6 L 13 4.8 L 12 LYM396 61900.1 72.8 L21 9.1 L 19 5.0 L 16 LYM396 61900.12 66.7 0.30 11 — — — 4.7 L 9 LYM39661901.1 — — — — — — 4.5 0.12 4 LYM396 61901.7 65.0 0.27 8 — — — 4.7 0.078 LYM396 61902.7 — — — — — — 4.9 0.29 12 LYM393 61610.6 80.6 0.10 3410.1 0.10 32 5.2 0.02 19 LYM393 61610.8 80.5 0.21 34 10.1 0.22 31 5.10.18 17 LYM393 61614.9 76.1 L 26 9.5 L 24 5.1 L 17 LYM380 61825.4 — — —— — — 4.8 0.20 12 LYM380 61825.5 64.1 0.24 7 — — — 4.8 0.01 10 LYM38061828.3 88.0 0.18 46 11.0 0.19 44 5.3 0.22 22 LYM380 61828.5 69.1 0.1815 8.6 0.21 13 4.9 0.19 14 LYM377 61592.5 96.2 0.13 60 12.0 0.14 57 5.60.10 30 LYM377 61594.1 89.9 0.13 49 11.2 0.13 47 5.4 0.14 25 LYM37761594.12 70.5 L 17 8.8 L 15 4.8 L 11 LYM377 61594.8 83.2 0.16 38 10.40.16 36 5.2 0.17 21 LYM376 61835.2 80.4 0.30 34 — — — 5.2 0.27 19 LYM37661835.3 67.2 0.19 12 8.4 0.24 10 — — — LYM376 61836.1 — — — — — — 4.60.15 7 LYM376 61837.1 73.9 0.01 23 9.2 0.02 21 5.0 L 15 LYM376 61839.478.5 0.17 30 9.8 0.18 28 5.0 0.19 15 LYM375 61754.4 65.1 0.23 8 — — —4.6 0.14 5 LYM375 61755.4 — — — — — — 4.5 0.30 3 LYM375 61756.1 91.70.11 52 11.5 0.12 50 5.6 0.15 30 LYM375 61758.1 67.9 L 13 8.5 L 11 4.60.08 7 LYM372 62002.1 75.2 0.01 25 9.4 0.01 23 5.0 L 15 LYM372 62003.2 —— — — — — 4.5 0.11 4 LYM372 62003.6 67.5 L 12 8.4 0.01 10 4.7 0.21 9LYM372 62006.4 66.5 0.27 11 — — — 4.6 0.03 7 LYM366 61906.15 74.4 L 249.3 L 22 5.0 L 17 LYM366 61906.9 77.6 L 29 9.7 L 27 5.0 0.06 16 LYM36661910.6 89.7 0.10 49 11.2 0.10 47 5.6 L 30 LYM366 61910.8 70.4 L 17 8.8L 15 4.8 L 12 LYM361 61795.1 76.5 0.23 27 9.6 0.24 25 5.0 0.14 16 LYM36161795.2 73.2 0.15 22 9.2 0.17 20 4.9 0.01 14 LYM361 61797.1 75.5 L 269.4 L 23 5.0 L 16 LYM354 61800.4 79.8 0.06 33 10.0 0.07 30 5.2 0.10 19LYM354 61801.3 85.4 0.27 42 10.7 0.29 40 — — — LYM354 61803.4 79.8 0.0233 10.0 0.02 30 5.2 0.02 20 LYM354 61804.3 84.0 0.19 40 10.5 0.20 37 5.30.05 23 LYM354 61804.4 88.4 0.15 47 11.1 0.15 45 5.4 0.10 25 LYM34661616.15 71.2 L 18 9.5 0.16 25 5.1 0.18 18 LYM346 61616.16 83.1 0.12 3810.4 0.13 36 5.2 0.17 20 LYM346 61616.9 75.3 0.10 25 9.4 0.11 23 5.1 L17 LYM346 61617.9 105.6 0.13 75 13.2 0.13 73 5.8 0.06 35 LYM346 61618.469.0 L 15 8.6 L 13 4.7 L 9 LYM344 61788.2 78.2 0.26 30 9.8 0.27 28 5.10.10 17 LYM344 61788.4 80.4 0.09 34 10.1 0.10 31 5.2 0.06 19 LYM34461790.1 84.1 L 40 10.5 L 37 5.3 L 23 LYM344 61790.3 72.9 0.26 21 9.10.29 19 4.9 0.06 13 LYM334 61942.6 68.7 0.17 14 8.6 0.21 12 4.9 0.15 13LYM334 61942.7 72.8 0.10 21 9.1 0.11 19 4.9 L 13 LYM334 61942.8 78.10.25 30 9.8 0.27 28 5.0 0.21 15 LYM334 61943.12 74.7 0.04 24 9.3 0.04 225.0 0.01 16 LYM334 61947.7 68.7 0.18 14 8.6 0.22 12 4.7 0.08 9 LYM33061840.1 82.7 0.11 37 10.3 0.11 35 5.3 0.02 23 LYM330 61841.4 71.1 0.2118 8.9 0.23 16 4.9 0.02 14 LYM330 61842.4 73.6 0.21 22 9.2 0.23 20 4.90.23 14 LYM330 61844.3 — — — — — — 4.8 0.23 11 LYM327 61846.1 86.9 0.0244 10.9 0.02 42 5.3 L 23 LYM327 61846.3 71.5 0.15 19 8.9 0.17 17 4.70.02 9 LYM327 61848.2 — — — — — — 4.6 0.01 5 LYM320 61851.2 74.8 0.12 249.4 0.13 22 4.9 0.17 13 LYM320 61852.4 80.9 L 34 10.1 L 32 5.3 L 21LYM320 61853.2 70.7 L 18 8.8 0.01 16 4.9 L 14 LYM320 61854.2 75.1 0.0425 9.4 0.05 23 5.0 0.02 16 LYM319 61918.5 78.9 0.05 31 9.9 0.06 29 5.00.10 16 LYM319 61920.6 — — — — — — 5.0 0.21 15 LYM319 61920.7 64.8 0.208 8.1 0.29 6 4.7 0.21 10 LYM319 61921.5 78.8 0.10 31 9.9 0.11 29 5.00.14 16 LYM319 61921.7 — — — — — — 4.6 L 7 LYM318 61622.2 70.6 0.29 17 —— — 4.9 0.20 14 LYM318 61623.2 68.3 0.26 14 — — — 4.8 0.04 11 LYM31861623.3 65.4 0.18 9 8.2 0.25 7 4.6 0.03 7 LYM313 61628.2 78.0 0.06 309.8 0.06 28 5.1 L 18 LYM313 61629.1 71.0 0.08 18 8.9 0.10 16 4.9 0.01 12LYM313 61630.1 — — — — — — 5.1 0.30 17 LYM313 61630.2 80.2 L 33 10.0 L31 5.1 L 18 LYM313 61631.3 71.1 0.25 18 8.9 0.28 16 4.8 0.12 12 LYM31061634.4 70.2 L 17 8.8 L 15 4.8 L 12 LYM310 61637.2 75.4 L 25 9.4 L 235.1 L 17 LYM310 61637.4 86.4 L 44 10.8 L 41 5.4 L 26 LYM310 61638.4 83.3L 38 10.4 L 36 5.3 L 22 LYM301 61912.3 74.4 0.23 24 9.3 0.24 22 5.1 0.1519 LYM301 61913.3 76.1 0.15 27 9.5 0.17 24 5.0 0.02 16 LYM301 61914.180.1 0.02 33 10.0 0.03 31 5.3 L 21 LYM301 61914.2 72.1 L 20 9.0 L 18 4.8L 10 LYM301 61916.2 — — — — — — 4.7 0.05 9 LYM300 61749.4 74.7 L 24 9.3L 22 5.0 L 16 LYM300 61750.3 77.3 0.06 28 9.7 0.07 26 5.2 0.05 19 LYM30061750.4 — — — — — — 5.3 0.23 22 LYM300 61752.3 79.5 0.04 32 9.9 0.04 305.1 0.07 18 LYM299 61806.2 75.4 0.07 25 9.4 0.08 23 5.1 0.03 19 LYM29961806.4 87.1 0.05 45 10.9 0.06 42 5.4 L 25 LYM299 61807.4 73.1 0.09 219.1 0.10 19 5.0 0.07 15 LYM299 61808.4 74.7 0.29 24 — — — 4.9 0.15 13LYM299 61809.2 78.2 L 30 9.8 L 28 5.2 0.08 21 CONT. — 60.2 — — 7.6 — —4.3 — — LYM507 62272.9 36.5 0.26 4 — — — — — — LYM507 62273.12 39.3 L 124.9 0.03 10 4.0 0.18 3 LYM507 62275.5 42.6 0.20 21 5.3 0.22 19 4.1 0.057 LYM505 62278.6 38.7 0.02 10 4.8 0.07 8 — — — LYM505 62279.5 44.8 0.0927 5.6 0.08 25 4.2 0.10 9 LYM500 62365.1 43.4 L 23 5.4 L 21 4.2 0.01 8LYM499 62096.2 43.4 0.09 23 5.4 0.09 21 4.2 0.12 9 LYM498 62078.2 38.60.11 10 4.8 0.17 8 4.1 0.28 5 LYM498 62078.4 37.0 0.16 5 — — — — — —LYM494 62413.4 39.1 0.30 11 — — — — — — LYM494 62414.5 38.3 0.09 9 4.80.18 7 — — — LYM489 61833.3 43.1 L 22 5.4 L 20 4.1 0.01 7 LYM466 62214.737.3 0.09 6 4.7 0.28 4 — — — LYM464 62068.2 36.9 0.18 5 — — — — — —LYM461 62219.2 37.9 0.13 8 4.7 0.25 6 — — — LYM454 62196.4 — — — — — —4.1 0.14 6 LYM438 62185.4 37.7 0.23 7 — — — — — — LYM437 62406.3 37.30.14 6 — — — — — — LYM437 62406.6 — — — — — — 4.1 0.05 6 LYM404 62244.136.8 0.25 5 — — — — — — LYM404 62246.12 — — — — — — 4.0 0.27 4 LYM38762102.4 38.6 0.02 10 4.8 0.08 8 — — — LYM363 62071.1 40.5 L 15 5.1 L 134.0 0.25 5 LYM348 62190.2 37.6 0.30 7 — — — — — — LYM343 62458.4 40.80.23 16 5.1 0.25 14 — — — LYM323 62356.5 37.3 0.09 6 4.7 0.28 4 — — —LYM322 62332.2 41.1 0.27 17 5.1 0.30 15 4.1 0.21 6 LYM322 62334.5 44.20.23 26 5.5 0.24 24 4.2 0.27 9 LYM322 62336.1 43.8 0.12 24 5.5 0.12 224.2 0.17 9 LYM321 62262.12 44.3 0.10 26 5.5 0.10 24 4.4 L 13 LYM32162264.12 40.8 0.07 16 5.1 0.08 14 3.9 0.30 3 LYM317 62252.11 42.3 0.2920 — — — — — — CONT. — 35.2 — — 4.5 — — 3.8 — — Table 39.“CONT.”—Control; “Ave.”—Average; “% Incr.” = % increment;“p-val.”—p-value, L—p < 0.01. The transgenes were under thetranscriptional regulation of the new At6669 promoter (SEQ ID NO: 4668).

TABLE 40 Genes showing improved plant performance at Normal growthconditions under regulation of At6669 promoter RGR Of Leaf RGR Of PlotRGR Of Rosette Number Coverage Diameter Gene P- % P- % P- % Name Event #Ave. Val. Incr. Ave. Val. Incr. Ave. Val. Incr. LYM445 62796.4 — — — 8.30.29 16 — — — LYM445 62797.2 0.7 0.23 18 — — — — — — LYM436 62812.4 0.70.25 18 — — — — — — LYM435 62875.1 — — — 8.8 0.15 23 — — — LYM43262946.1 0.8 0.07 29 — — — — — — LYM428 63411.2 0.8 0.08 29 9.4 0.05 310.5 0.09 17 LYM419 62778.2 — — — — — — 0.5 0.24 12 LYM414 62968.1 — — —8.4 0.26 18 0.5 0.27 11 LYM410 62964.2 0.7 0.26 19 — — — — — — LYM39862886.2 0.8 0.20 21 — — — — — — LYM394 62913.2 0.8 0.20 22 — — — — — —LYM365 62721.2 0.8 0.23 20 — — — — — — CONT. — 0.6 — — 7.1 — — 0.4 — —LYM510 62475.1 — — — 7.2 0.14 15 0.4 0.25 7 LYM501 62535.2 — — — 7.10.24 12 0.4 0.15 9 LYM501 62537.1 — — — 8.1 0.01 29 0.5 L 21 LYM48862564.6 — — — — — — 0.4 0.25 7 LYM488 62566.1 — — — — — — 0.4 0.28 7LYM471 62657.1 0.7 0.15 17 — — — — — — LYM471 62658.1 — — — 7.0 0.28 11— — — LYM471 62658.2 0.8 0.05 23 8.1 0.01 29 0.5 L 20 LYM465 62340.2 — —— — — — 0.4 0.09 11 LYM465 62342.4 — — — 7.6 0.05 21 0.4 0.22 8 LYM46062423.3 — — — — — — 0.4 0.25 8 LYM460 62425.1 — — — — — — 0.4 0.09 11LYM455 62704.2 0.7 0.24 16 — — — — — — LYM455 62705.5 0.8 0.03 28 8.5 L36 0.5 L 18 LYM455 62708.5 0.7 0.26 14 7.0 0.26 12 — — — LYM451 62486.60.7 0.21 17 — — — — — — LYM451 62488.3 — — — 7.7 0.04 22 0.4 0.01 16LYM447 62683.1 0.7 0.22 14 — — — — — — LYM446 62495.1 — — — 7.6 0.05 210.4 0.11 10 LYM444 62628.4 — — — 7.0 0.25 12 0.4 0.15 9 LYM416 62663.1 —— — 7.1 0.18 14 0.4 0.14 9 LYM406 62562.1 — — — — — — 0.4 0.21 8 LYM40662562.2 — — — 7.1 0.18 14 0.4 0.10 10 LYM388 62540.3 — — — — — — 0.40.18 8 LYM388 62543.1 — — — — — — 0.4 0.19 8 LYM374 62505.2 0.7 0.18 16— — — — — — LYM364 62694.1 — — — 7.4 0.10 18 0.4 0.20 9 LYM362 62344.6 —— — — — — 0.4 0.25 7 LYM362 62349.4 — — — 7.5 0.07 19 0.4 0.12 10 LYM35962329.1 — — — 7.9 0.01 26 0.4 0.01 16 LYM355 62450.6 — — — 7.5 0.07 200.4 0.02 15 LYM355 62451.4 — — — 7.1 0.21 13 — — — LYM353 62675.4 — — —7.6 0.05 21 0.4 0.04 13 LYM353 62677.5 — — — — — — 0.4 0.25 7 LYM35362677.6 — — — 7.0 0.29 11 — — — LYM351 62529.2 — — — 7.9 0.01 27 0.40.04 13 LYM351 62530.3 0.8 0.07 22 8.4 L 34 0.4 0.03 14 LYM341 62484.10.8 0.07 22 7.9 0.02 25 0.4 0.10 10 LYM339 62671.2 — — — 7.3 0.11 17 0.40.19 8 LYM339 62671.3 — — — 7.2 0.15 15 — — — LYM332 62554.3 — — — 8.0 L28 0.4 0.06 11 LYM325 62689.2 — — — — — — 0.4 0.19 8 LYM306 62433.1 — —— 7.5 0.06 20 0.4 0.04 13 LYM305 62518.1 — — — 7.4 0.10 18 0.4 0.24 7LYM305 62519.4 — — — 8.1 L 29 0.4 0.03 13 LYM303 62523.1 — — — 7.3 0.1217 0.4 0.17 9 CONT. — 0.6 — — 6.3 — — 0.4 — — LYM503 61581.5 0.8 0.21 12— — — — — — LYM503 61581.6 — — — 8.1 0.28 13 0.4 0.27 10 LYM503 61584.10.8 0.30 10 — — — 0.4 0.12 17 LYM503 61584.7 0.8 0.09 16 — — — — — —LYM495 61744.1 — — — 8.8 0.06 23 0.4 0.08 17 LYM493 61966.4 — — — — — —0.4 0.22 12 LYM493 61969.12 — — — 8.9 0.06 24 0.4 0.03 22 LYM493 61969.8— — — — — — 0.4 0.27 11 LYM480 61960.6 — — — — — — 0.4 0.28 10 LYM48061962.7 — — — — — — 0.4 0.29 10 LYM474 61975.8 — — — — — — 0.4 0.28 10LYM474 61976.8 — — — — — — 0.4 0.18 12 LYM473 61783.2 — — — 9.1 0.04 280.4 0.01 28 LYM473 61783.4 — — — — — — 0.4 0.01 25 LYM458 61812.4 — — —— — — 0.4 0.09 17 LYM458 61813.3 — — — — — — 0.4 0.08 16 LYM458 61814.1— — — 8.5 0.14 19 0.4 0.17 14 LYM458 61816.4 — — — 8.1 0.27 14 0.4 0.0519 LYM456 61587.8 0.8 0.28 10 — — — 0.4 0.29 10 LYM456 61588.5 — — — — —— 0.4 0.15 14 LYM456 61588.7 0.8 0.25 11 9.1 0.04 27 0.4 0.04 22 LYM45661589.4 — — — 8.2 0.24 15 0.4 0.23 12 LYM456 61590.8 — — — — — — 0.40.12 14 LYM453 61984.7 — — — — — — 0.4 0.04 20 LYM453 61986.6 — — — 11.0L 53 0.5 L 40 LYM453 61988.6 — — — 9.9 L 39 0.4 L 33 LYM442 61980.5 — —— 8.3 0.19 17 0.4 L 28 LYM440 61936.6 — — — 9.0 0.05 26 0.4 0.03 22LYM440 61937.6 — — — 8.9 0.05 25 0.4 0.02 22 LYM440 61937.8 — — — — — —0.4 0.11 15 LYM415 61598.5 0.8 0.05 19 8.7 0.08 22 0.4 0.22 11 LYM41561598.7 — — — — — — 0.4 0.24 12 LYM415 61600.5 — — — 8.3 0.17 17 0.40.03 22 LYM415 61602.6 — — — 8.3 0.16 17 0.4 0.05 18 LYM415 61602.8 — —— — — — 0.4 0.06 19 LYM409 61998.2 — — — 10.4 L 46 0.4 0.01 30 LYM40961998.6 — — — 9.3 0.02 31 0.4 L 36 LYM409 61999.2 — — — 9.5 0.01 33 0.4L 29 LYM409 61999.3 — — — 9.1 0.05 27 0.4 0.02 26 LYM409 61999.5 — — —8.3 0.18 17 0.4 0.07 18 LYM396 61900.1 — — — 8.8 0.06 24 0.4 L 27 LYM39661900.12 — — — 8.1 0.25 14 0.4 0.05 19 LYM396 61901.1 — — — — — — 0.40.21 12 LYM396 61901.7 — — — — — — 0.4 0.02 23 LYM396 61902.7 — — — 8.80.08 24 0.4 0.01 26 LYM393 61610.6 — — — 9.9 L 38 0.4 L 29 LYM39361610.8 — — — 9.6 L 35 0.4 0.02 23 LYM393 61613.7 — — — — — — 0.4 0.2510 LYM393 61614.9 — — — 9.1 0.03 28 0.4 L 26 LYM380 61824.2 — — — 9.00.07 26 0.4 0.05 22 LYM380 61825.4 — — — — — — 0.4 L 26 LYM380 61825.50.8 0.30 11 — — — 0.4 0.01 25 LYM380 61828.3 — — — 10.8 L 52 0.4 L 36LYM380 61828.5 — — — 8.5 0.12 20 0.4 L 32 LYM377 61592.5 0.8 0.22 1111.9 L 66 0.5 L 46 LYM377 61594.1 — — — 10.8 L 52 0.4 L 32 LYM37761594.12 — — — 8.5 0.11 19 0.4 0.06 17 LYM377 61594.8 — — — 10.1 L 410.4 L 30 LYM376 61835.2 — — — 9.7 0.02 35 0.4 0.01 30 LYM376 61835.3 0.80.25 11 8.1 0.27 13 — — — LYM376 61836.1 — — — — — — 0.4 0.16 14 LYM37661837.1 — — — 8.8 0.06 23 0.4 0.03 20 LYM376 61839.4 — — — 9.3 0.02 310.4 0.08 17 LYM375 61756.1 0.9 0.01 22 11.2 L 57 0.5 L 44 LYM375 61758.1— — — 8.3 0.16 16 0.4 0.10 16 LYM372 62002.1 — — — 9.0 0.03 27 0.4 0.0320 LYM372 62003.6 — — — 8.1 0.26 14 0.4 0.21 12 LYM372 62006.4 — — — — —— 0.4 0.11 14 LYM366 61906.15 — — — 9.0 0.04 26 0.4 L 30 LYM366 61906.9— — — 9.4 0.01 32 0.4 0.03 22 LYM366 61910.6 — — — 11.1 L 56 0.5 L 53LYM366 61910.7 — — — 8.5 0.16 19 0.4 0.07 19 LYM366 61910.8 — — — 8.50.14 18 0.4 0.08 17 LYM361 61794.3 — — — 9.2 0.04 29 0.4 0.03 24 LYM36161795.1 — — — 9.2 0.03 29 0.4 0.02 24 LYM361 61795.2 — — — 8.8 0.08 230.4 0.13 16 LYM361 61796.4 — — — 8.8 0.12 23 0.4 0.04 25 LYM361 61797.1— — — 9.1 0.03 27 0.4 0.05 19 LYM354 61800.4 0.8 0.06 17 9.8 L 38 0.4 L33 LYM354 61801.3 — — — 10.4 L 46 0.4 L 34 LYM354 61803.4 — — — 9.7 L 370.4 L 32 LYM354 61804.3 — — — 10.1 L 42 0.4 L 33 LYM354 61804.4 — — —10.7 L 49 0.4 L 36 LYM346 61616.15 — — — 8.7 0.06 23 0.4 L 29 LYM34661616.16 — — — 10.0 L 40 0.4 0.01 27 LYM346 61616.9 — — — 9.2 0.03 280.4 L 28 LYM346 61617.9 — — — 13.0 L 83 0.5 L 52 LYM346 61618.4 — — —8.4 0.15 18 0.4 0.04 19 LYM344 61788.2 — — — 9.4 0.02 32 0.4 0.03 23LYM344 61788.4 — — — 9.8 L 37 0.4 L 32 LYM344 61790.1 — — — 10.1 L 410.4 L 30 LYM344 61790.3 0.8 0.27 12 9.0 0.05 26 0.4 L 26 LYM344 61791.1— — — 8.2 0.23 15 0.4 0.04 23 LYM334 61942.6 — — — 8.4 0.15 18 0.4 L 28LYM334 61942.7 — — — 8.5 0.13 19 0.4 0.04 19 LYM334 61942.8 — — — 9.40.02 32 0.4 0.03 22 LYM334 61943.12 — — — 9.0 0.04 26 0.4 L 26 LYM33461947.7 — — — 8.3 0.21 17 0.4 0.07 18 LYM330 61840.1 — — — 10.1 L 41 0.4L 36 LYM330 61841.4 — — — 8.5 0.13 19 0.4 0.03 21 LYM330 61842.4 — — —8.9 0.06 25 0.4 0.05 20 LYM330 61844.3 — — — 8.3 0.21 16 0.4 0.21 14LYM327 61846.1 — — — 10.5 L 48 0.4 L 37 LYM327 61846.3 — — — 8.5 0.12 200.4 0.14 14 LYM327 61847.1 0.8 0.29 9 8.2 0.24 15 — — — LYM327 61848.2 —— — — — — 0.4 0.16 13 LYM320 61851.2 — — — 8.9 0.05 24 0.4 0.08 16LYM320 61852.4 — — — 9.8 L 38 0.4 L 37 LYM320 61853.2 — — — 8.6 0.10 200.4 L 28 LYM320 61854.2 — — — 9.1 0.03 27 0.4 L 27 LYM319 61918.5 — — —9.5 0.01 34 0.4 0.01 26 LYM319 61920.6 — — — 8.9 0.07 25 0.4 L 29 LYM31961920.7 — — — — — — 0.4 L 27 LYM319 61921.5 — — — 9.6 0.01 35 0.4 L 29LYM319 61921.7 — — — — — — 0.4 0.06 19 LYM318 61622.2 — — — 8.6 0.12 200.4 0.01 27 LYM318 61623.2 — — — 8.2 0.24 15 0.4 0.05 20 LYM318 61623.3— — — — — — 0.4 0.06 18 LYM318 61625.4 — — — — — — 0.4 0.24 14 LYM31861625.6 — — — — — — 0.4 0.06 23 LYM313 61628.2 — — — 9.5 0.01 33 0.4 L28 LYM313 61629.1 — — — 8.5 0.13 19 0.4 0.04 19 LYM313 61630.1 — — — 9.00.06 26 0.4 0.02 25 LYM313 61630.2 — — — 9.8 L 37 0.4 L 26 LYM31361631.3 — — — 8.6 0.11 21 0.4 0.06 19 LYM310 61634.4 — — — 8.4 0.15 180.4 0.03 21 LYM310 61637.2 — — — 9.1 0.03 27 0.4 L 25 LYM310 61637.4 — —— 10.6 L 49 0.5 L 40 LYM310 61638.4 — — — 10.1 L 41 0.4 L 29 LYM30161912.3 — — — 9.2 0.03 29 0.5 L 37 LYM301 61913.3 — — — 9.4 0.02 31 0.4L 28 LYM301 61914.1 — — — 9.9 L 38 0.5 L 38 LYM301 61914.2 — — — 8.80.09 23 0.4 0.05 20 LYM301 61916.2 — — — — — — 0.4 0.01 24 LYM30061748.4 0.8 0.29 12 — — — — — — LYM300 61749.4 — — — 9.0 0.04 25 0.40.02 24 LYM300 61750.3 — — — 9.4 0.01 31 0.4 L 27 LYM300 61750.4 0.80.18 13 9.4 0.03 32 0.5 L 38 LYM300 61752.3 — — — 9.6 0.01 34 0.4 0.0124 LYM299 61806.2 — — — 9.2 0.02 29 0.4 L 34 LYM299 61806.4 — — — 10.6 L49 0.5 L 37 LYM299 61807.4 — — — 8.8 0.07 23 0.4 0.04 20 LYM299 61808.4— — — 9.0 0.05 26 0.4 0.03 21 LYM299 61809.2 — — — 9.6 L 34 0.4 L 36CONT. — 0.7 — — 7.1 — — 0.3 — — LYM509 62202.1 — — — 5.2 0.24 18 — — —LYM507 62275.5 — — — 5.3 0.15 21 — — — LYM505 62279.5 — — — 5.7 0.05 29— — — LYM500 62365.1 — — — 5.5 0.09 25 — — — LYM499 62096.2 — — — 5.60.08 26 — — — LYM489 61833.3 — — — 5.5 0.10 24 — — — LYM407 62142.2 0.70.26 19 — — — — — — LYM363 62071.1 — — — 5.2 0.21 18 — — — LYM34362458.4 — — — 5.1 0.25 16 — — — LYM322 62332.2 — — — 5.2 0.19 18 — — —LYM322 62334.5 — — — 5.6 0.07 27 — — — LYM322 62336.1 — — — 5.6 0.07 26— — — LYM321 62262.12 — — — 5.7 0.05 29 0.4 0.08 19 LYM321 62264.12 — —— 5.1 0.25 16 — — — LYM317 62252.11 — — — 5.4 0.13 22 — — — CONT. — 0.6— — 4.4 — — 0.3 — — Table 40. “CONT.”—Control; “Ave.”—Average; “% Incr.”= % increment; “p-val.”—p-value, L—p < 0.01. The transgenes were underthe transcriptional regulation of the new At6669 promoter (SEQ ID NO:4668).

TABLE 41 Genes showing improved plant performance at Normal growthconditions under regulation of At6669 promoter Blade Relative Area BladeRelative Area Blade Relative Area TP2 TP3 TP4 Gene P- % P- % P- % NameEvent # Ave. Val. Incr. Ave. Val. Incr. Ave. Val. Incr. LYM370 62350.2 —— — — — — 87.93 0.32 1 LYM370 62351.1 — — — 91.8 0.09 3 89.52 0.24 3LYM370 62351.3 88.81 0.23 3 — — — — — — LYM370 62351.4 88.75 0.03 391.66 0.01 3 89.26 0.01 3 LYM441 62361.3 89.67 0.1 4 — — — — — — LYM44162364.1 — — — 90 0.14 1 — — — LYM441 62364.2 89.14 0.13 3 92 0.03 389.14 0.02 3 LYM443 62498.5 — — — 91 0.04 2 88.33 0.36 2 LYM443 62501.1— — — 92 L 3 88.91 0.02 2 LYM443 62502.2 — — — 91 L 3 89.91 L 3 CONTROL— 86.46 — — 89 — — 86.94 — — LYM345 62937.2 92.42 0.17 1 — — — — — —LYM345 62938.4 — — — 92 0.5 1 — — — LYM345 62938.6 93.25 0.33 2 93 0 389.98 0.6 1 LYM345 62940.1 93.49 0.26 2 93.74 0.14 3 90.89 0.11 2 LYM40563205.2 — — — 92.7 0.24 2 91.01 0.3 2 LYM405 63205.4 93.15 0.3 2 93.170.14 2 — — — LYM405 63206.2 93.47 0.03 2 93.39 0.03 3 90.67 0.04 2LYM405 63209.1 — — — 93 0.01 2 91.28 0.19 2 LYM457 62906.1 — — — 91.990.2 1 — — — LYM457 62906.3 92.31 0.08 1 92.06 0.16 1 — — — LYM45762907.4 92.89 0.38 1 92.65 0.3 2 90.38 0.35 1 LYM457 62910.1 — — — 92.360.15 2 90.39 0.45 1 CONTROL — 91.68 — — 90.94 — 0 89.18 — — Table 41.“CONT.”—Control; “Ave.”—Average; “% Incr.” = % increment;“p-val.”—p-value, L—p < 0.01. The transgenes were under thetranscriptional regulation of the new At6669 promoter (SEQ ID NO: 4668).

TABLE 42 Genes showing improved plant performance at Normal growthconditions under regulation of At6669 promoter Petiole Relative PetioleRelative Petiole Relative Area TP2 Area TP3 Area TP4 Gene P- % P- % P- %Name Event # Ave. Val. Incr. Ave. Val. Incr. Ave. Val. Incr. LYM37062350.2 15.56 0.28 15 — — — — — — LYM370 62353.4 — — — 12.84 0.11 1814.78 0.04 13 LYM441 62360.2 14.96 0.44 11 11.92 0.29 9 — — — LYM44362498.3 15.49 0.05 14 12.49 0.3 14 — — — LYM443 62498.4 — — — — — —14.01 0.35 7 CONTROL — 13.54 — — 10.92 — — 13.06 — — LYM345 62937.2 — —— — — — 12.25 0.07 13 LYM405 63209.3 10.1 0.6 21 — — — — — — LYM45762907.5 9.25 0.21 11 10.96 0.23 21 — — — CONTROL — 8.32 — — 9.06 — —10.82 — — Table 42. “CONT.”—Control; “Ave.” Average; “% Incr.” = %increment; “p-val.”—p-value, L—p < 0.01. The transgenes were under thetranscriptional regulation of the new At6669 promoter (SEQ ID NO: 4668).

Example 12 Evaluating Transgenic Arabidopsis Under Normal ConditionsUsing In Vitro Assays [Tissue Culture T2 and T1 Plants, TC-T2 and TC-T1Assays]

Surface sterilized seeds were sown in basal media [50% Murashige-Skoogmedium (MS) supplemented with 0.8% plant agar as solidifying agent] inthe presence of Kanamycin (used as a selecting agent). After sowing,plates were transferred for 2-3 days for stratification at 4° C. andthen grown at 25° C. under 12-hour light 12-hour dark daily cycles for 7to 10 days. At this time point, seedlings randomly chosen were carefullytransferred to plates containing ½ MS media (15 mM N). For experimentsperformed in T₂ lines, each plate contained 5 seedlings of the sametransgenic event, and 3-4 different plates (replicates) for each event.For each polynucleotide of the invention at least four-five independenttransformation events were analyzed from each construct. For experimentsperformed in T₁ lines, each plate contained 5 seedlings of 5 independenttransgenic events and 3-4 different plates (replicates) were planted. Intotal, for T₁ lines, 20 independent events were evaluated. Plantsexpressing the polynucleotides of the invention were compared to theaverage measurement of the control plants (empty vector or GUS reportergene under the same promoter) used in the same experiment.

Digital Imaging—

A laboratory image acquisition system, which consists of a digitalreflex camera (Canon EOS 300D) attached with a 55 mm focal length lens(Canon EF-S series), mounted on a reproduction device (Kaiser RS), whichincludes 4 light units (4×150 Watts light bulb) and located in adarkroom, was used for capturing images of plantlets sawn in agarplates.

The image capturing process was repeated every 3-4 days starting at day1 till day 10 (see for example the images in FIGS. 3A-F). An imageanalysis system was used, which consists of a personal desktop computer(Intel P4 3.0 GHz processor) and a public domain program—ImageJ 1.39[Java based image processing program which was developed at the U.S.National Institutes of Health and freely available on the internet atHypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/]. Images werecaptured in resolution of 10 Mega Pixels (3888×2592 pixels) and storedin a low compression JPEG (Joint Photographic Experts Group standard)format. Next, analyzed data was saved to text files and processed usingthe JMP statistical analysis software (SAS institute).

Seedling Analysis—

Using the digital analysis seedling data was calculated, including leafarea, root coverage and root length.

The relative growth rate for the various seedling parameters wascalculated according to the following formulas XIV, VI (described above)and XV.

Relative growth rate of leaf area=Regression coefficient of leaf areaalong time course.  Formula XIV:

Relative growth rate of root length=Regression coefficient of rootlength along time course.  Formula XV:

At the end of the experiment, plantlets were removed from the media andweighed for the determination of plant fresh weight. Plantlets were thendried for 24 hours at 60° C., and weighed again to measure plant dryweight for later statistical analysis. The fresh and dry weights areprovided for each Arabidopsis plant. Growth rate was determined bycomparing the leaf area coverage, root coverage and root length, betweeneach couple of sequential photographs, and results were used to resolvethe effect of the gene introduced on plant vigor under optimalconditions. Similarly, the effect of the gene introduced on biomassaccumulation, under optimal conditions, was determined by comparing theplants' fresh and dry weight to that of control plants (containing anempty vector or the GUS reporter gene under the same promoter). Fromevery construct created, 3-5 independent transformation events wereexamined in replicates.

Statistical Analyses—

To identify genes conferring significantly improved plant vigor orenlarged root architecture, the results obtained from the transgenicplants were compared to those obtained from control plants. To identifyoutperforming genes and constructs, results from the independenttransformation events tested were analyzed separately. To evaluate theeffect of a gene event over a control the data was analyzed by Student'st-test and the p value was calculated. Results were consideredsignificant if p<0.1. The JMP statistics software package was used(Version 5.2.1, SAS Institute Inc., Cary, N.C., USA).

Experimental Results:

Tables 43-45 summarize the observed phenotypes of transgenic plantsexpressing the gene constructs using the TC-T2 Assays.

The genes presented in Table 43 showed a significant improvement as theyproduced larger plant biomass (plant fresh and dry weight) in T2generation when grown under normal growth conditions, compared tocontrol plants. The genes were cloned under the regulation of aconstitutive promoter (At6669, SEQ ID NO:4668). The evaluation of eachgene was carried out by testing the performance of different number ofevents. Some of the genes were evaluated in more than one tissue cultureassay. The results obtained in these second experiments weresignificantly positive as well.

TABLE 43 Genes showing improved plant performance at Normal growthconditions under regulation of At6669 promoter Gene Dry Weight [mg]Fresh Weight [mg] Name Event # Ave. P-Val. % Incr. Ave. P-Val. % Incr.LYM490 62920.3 5.7 0.04 41 105.8 0.21 23 LYM448 62802.2 5.2 0.05 30102.5 0.30 19 LYM448 62805.2 5.2 0.02 29 103.2 0.19 20 LYM435 62872.17.6 0.04 88 156.9 0.06 82 LYM419 62775.1 — — — 100.9 0.11 17 LYM41962778.1 6.7 0.05 67 139.3 0.03 62 LYM401 62854.1 7.0 0.05 75 133.6 0.0955 LYM401 62856.1 6.0 0.09 50 124.1 0.13 44 LYM401 62856.4 4.5 0.17 13 —— — LYM401 62857.5 5.8 0.04 45 121.5 0.11 41 LYM395 62924.2 4.8 0.24 19— — — LYM395 62927.2 — — — 102.0 0.09 18 LYM395 62928.1 4.7 0.29 16 — —— LYM371 62847.1 — — — 123.9 0.03 44 LYM371 62847.2 6.2 L 54 130.6 0.0451 LYM371 62851.2 5.9 0.17 45 148.7 0.09 73 LYM367 62735.5 — — — 109.40.04 27 LYM367 62737.2 5.5 0.07 38 111.0 L 29 LYM367 62739.2 8.2 0.08104 161.0 0.11 87 LYM352 62880.3 5.6 0.17 40 — — — LYM352 62881.2 6.30.13 57 122.2 0.25 42 LYM328 62731.1 4.5 0.21 11 — — — LYM328 62732.7 —— — 111.6 0.01 29 LYM314 62859.1 — — — 94.2 0.22 9 LYM314 62862.1 5.80.21 43 137.8 0.07 60 CONT. — 4.0 — — 86.2 — — LYM504 63308.5 3.1 0.1916 — — — LYM504 63309.1 4.2 L 56 76.3 L 34 LYM504 63309.3 3.5 0.01 30 —— — LYM483 63629.1 3.7 0.07 36 91.0 0.16 59 LYM483 63629.2 4.5 L 65 79.2L 39 LYM467 63564.2 3.8 0.08 38 69.8 0.27 22 LYM467 63564.3 3.8 0.02 39— — — LYM467 63565.1 4.2 0.02 55 67.8 0.02 19 LYM467 63565.4 3.0 0.25 11— — — LYM463 63108.2 5.2 L 91 87.0 L 52 LYM463 63110.9 3.6 0.05 34 — — —LYM463 63111.12 3.7 L 35 63.3 0.13 11 LYM463 63112.13 3.1 0.26 15 — — —LYM463 63113.2 3.6 0.15 32 — — — LYM450 63126.2 4.4 L 63 74.6 L 31LYM450 63127.9 4.0 0.05 49 71.4 0.04 25 LYM450 63128.1 — — — 65.3 0.2514 LYM450 63130.4 3.9 0.08 43 78.3 0.25 37 LYM433 63300.3 3.8 0.09 4069.6 0.06 22 LYM433 63300.4 4.5 0.05 64 81.2 0.03 42 LYM433 63301.2 4.3L 60 74.1 0.04 30 LYM433 63301.3 3.3 0.06 23 78.9 0.22 38 LYM433 63302.14.3 L 60 84.7 0.12 48 LYM425 63132.9 4.1 0.03 52 73.9 0.05 29 LYM42563133.2 3.2 0.03 18 — — — LYM425 63134.2 5.0 0.06 83 89.7 0.03 57 LYM42362981.12 3.2 0.15 20 — — — LYM423 63056.3 3.7 0.04 37 — — — LYM42363058.2 4.0 0.02 47 70.5 0.09 24 LYM422 63313.3 4.2 0.06 54 — — — LYM40363621.3 3.9 0.19 42 69.6 0.20 22 LYM403 63624.4 3.3 0.09 21 69.0 0.09 21LYM392 63324.2 4.1 0.02 51 77.6 0.14 36 LYM392 63326.2 3.7 0.10 37 — — —LYM392 63328.4 4.6 L 69 87.8 0.09 54 LYM381 63550.2 3.2 0.13 17 — — —LYM381 63550.4 4.0 L 46 — — — LYM381 63552.1 3.5 0.01 30 — — — LYM33663068.2 3.3 0.01 21 — — — LYM336 63071.2 4.1 L 50 71.5 0.04 25 LYM33663072.1 3.8 0.10 40 74.0 0.18 30 LYM336 63073.2 4.8 0.03 76 81.8 0.03 43LYM336 63073.4 4.0 0.17 47 77.0 L 35 CONT. — 2.7 — — 57.1 — — LYM49561742.2 6.1 L 100 103.1 L 81 LYM495 61743.2 3.9 0.23 27 — — — LYM49561744.1 4.1 0.15 34 74.6 0.13 31 LYM495 61744.3 5.1 0.04 68 89.9 0.03 58LYM495 61746.2 4.0 0.09 32 65.2 0.29 15 LYM475 63096.13 4.2 0.04 36 73.20.10 29 LYM475 63096.3 4.0 0.03 30 69.6 0.04 22 LYM475 63097.4 3.7 0.2620 65.7 0.12 15 LYM475 63098.1 5.1 L 67 77.8 0.03 37 LYM475 63100.1 3.70.13 21 64.8 0.12 14 LYM473 61783.4 6.1 L 99 104.3 0.04 83 LYM47361784.3 3.8 0.11 24 — — — LYM473 61786.1 4.3 0.02 42 68.8 0.11 21 LYM47263114.2 6.6 L 117 113.8 0.01 100 LYM472 63117.5 4.5 0.01 48 73.7 L 30LYM472 63117.6 4.8 0.03 57 78.1 L 37 LYM472 63118.8 4.8 0.05 57 90.00.05 58 LYM458 61812.2 4.1 0.13 35 66.4 0.15 17 LYM458 61814.1 8.0 0.01162 134.3 0.01 136 LYM413 61819.2 6.0 0.03 97 98.7 0.05 74 LYM41361819.3 4.8 0.06 56 82.7 0.05 45 LYM413 61822.3 5.0 L 66 87.3 0.01 54LYM413 61823.1 7.1 L 134 124.4 L 119 LYM400 63121.22 4.1 L 34 68.1 0.0720 LYM400 63124.13 5.5 L 82 91.9 0.01 62 LYM380 61824.2 8.1 0.02 167136.1 0.02 139 LYM380 61825.2 5.3 L 74 89.1 L 57 LYM380 61825.5 6.0 L 95101.3 L 78 LYM380 61828.3 5.4 L 77 95.5 L 68 LYM376 61835.2 4.4 0.06 4380.2 0.01 41 LYM376 61835.3 4.3 L 41 71.7 0.06 26 LYM376 61836.1 4.70.03 53 82.0 0.04 44 LYM376 61839.4 5.5 0.05 82 94.8 0.04 67 LYM35461804.3 5.6 0.02 85 103.9 0.04 83 LYM354 61804.4 4.8 L 57 83.7 L 47LYM344 61788.2 5.0 0.07 66 88.2 0.04 55 LYM344 61788.4 4.6 0.05 52 87.80.04 54 LYM344 61790.1 4.2 0.13 38 76.2 0.11 34 LYM344 61790.3 5.7 L 8794.7 L 66 LYM330 61840.1 5.5 L 80 96.4 L 70 LYM330 61840.3 3.6 0.20 20 —— — LYM330 61841.4 4.0 0.07 33 65.1 0.16 14 LYM330 61842.4 5.4 0.11 7785.3 0.13 50 LYM330 61844.3 7.2 0.09 135 115.5 0.10 103 LYM327 61846.18.7 0.06 186 145.6 0.05 156 LYM327 61846.3 7.2 L 137 122.3 L 115 LYM32761847.1 6.0 0.09 98 104.3 0.08 83 LYM327 61848.2 3.9 0.10 28 68.8 0.0521 LYM327 61849.1 5.9 L 93 95.6 0.03 68 LYM309 63102.3 7.1 L 132 121.1 L113 LYM309 63103.27 5.1 0.02 67 79.4 0.09 40 LYM309 63104.16 7.8 L 155137.5 L 142 LYM309 63104.3 4.6 L 50 76.3 0.01 34 LYM299 61806.4 3.5 0.2814 66.5 0.07 17 LYM299 61807.3 6.1 0.02 100 101.7 0.04 79 LYM299 61808.45.8 L 91 100.2 0.01 76 LYM299 61809.2 6.6 0.01 116 122.8 L 116 CONT. —3.1 — — 56.9 — — LYM493 61966.4 5.3 L 86 103.6 L 81 LYM493 61967.6 3.60.13 24 81.5 0.02 43 LYM493 61968.6 3.5 0.08 23 — — — LYM493 61968.8 — —— 74.2 0.21 30 LYM493 61969.8 5.2 L 81 104.7 L 83 LYM480 61960.6 5.80.02 100 104.8 0.02 83 LYM480 61961.1 5.2 L 83 103.1 L 80 LYM480 61962.85.2 L 82 87.9 0.06 54 LYM474 61972.5 5.2 0.09 81 100.8 0.12 76 LYM47461977.6 3.6 0.06 25 69.8 0.11 22 LYM453 61984.7 3.3 0.14 16 71.3 0.19 25LYM453 61984.9 5.5 0.05 91 110.5 0.07 93 LYM453 61985.4 6.9 L 138 131.5L 130 LYM453 61986.6 5.6 0.05 96 119.1 0.01 108 LYM442 61979.5 5.5 L 92105.6 L 85 LYM442 61979.6 4.4 0.03 52 80.9 0.01 42 LYM442 61980.5 4.0 L40 82.4 L 44 LYM442 61983.7 6.8 L 137 126.8 L 122 LYM440 61936.6 3.8 L31 74.0 0.06 29 LYM440 61937.6 3.9 0.19 34 84.9 0.09 49 LYM440 61937.84.7 0.03 63 93.1 0.08 63 LYM440 61939.6 5.2 0.02 83 102.4 L 79 LYM40961997.1 — — — 75.1 0.26 31 LYM409 61997.2 4.5 0.01 57 86.9 0.01 52LYM409 61998.2 — — — 74.8 0.18 31 LYM409 61998.6 5.3 L 83 116.4 L 104LYM409 61999.3 4.4 0.09 52 72.7 0.18 27 LYM396 61900.7 4.3 L 50 82.1 L44 LYM396 61901.1 3.5 0.17 23 78.0 L 36 LYM396 61901.7 5.6 L 95 109.1 L91 LYM396 61902.7 3.8 0.22 30 80.0 0.02 40 LYM396 61902.8 — — — 73.00.06 28 LYM372 62002.1 4.1 L 43 79.4 0.02 39 LYM372 62003.2 — — — 65.90.22 15 LYM372 62003.6 3.3 0.29 15 — — — LYM372 62004.2 3.3 0.21 16 67.40.08 18 LYM372 62006.4 4.0 L 38 77.0 0.02 35 LYM366 61906.15 5.6 L 95106.2 L 86 LYM366 61910.6 3.5 0.02 23 — — — LYM366 61910.7 3.9 0.22 3780.8 0.15 41 LYM366 61910.8 3.2 0.25 11 — — — LYM334 61942.6 4.1 0.01 4380.8 L 41 LYM334 61942.7 5.4 0.03 87 109.1 0.01 91 LYM334 61943.12 5.40.01 89 118.0 L 107 LYM334 61947.7 3.5 0.22 23 70.4 0.10 23 LYM31961920.6 — — — 66.0 0.17 16 LYM319 61921.5 3.8 0.03 32 73.5 0.04 29LYM301 61913.4 3.5 0.25 21 66.1 0.15 16 LYM301 61914.1 3.8 0.14 31 71.30.24 25 LYM301 61916.2 3.3 0.20 14 — — — CONT. — 2.9 — — 57.1 — — LYM48363625.2 5.3 0.07 40 102.4 0.01 30 LYM483 63626.7 4.7 0.07 23 — — —LYM467 63563.3 — — — 93.8 0.26 19 LYM467 63564.3 5.8 0.02 54 110.3 0.1440 LYM467 63565.4 4.6 0.17 21 97.0 0.27 23 LYM463 63113.2 5.9 0.03 55129.0 L 63 LYM450 63126.2 4.6 0.19 22 97.0 0.09 23 LYM450 63127.9 4.80.08 26 99.7 0.10 26 LYM433 63300.3 4.9 0.01 29 103.7 0.18 31 LYM43363300.4 5.9 L 55 118.7 L 50 LYM433 63301.3 5.7 L 50 102.1 0.02 29 LYM42563132.9 4.9 0.17 30 96.0 0.20 22 LYM425 63133.2 — — — 96.7 0.15 22LYM425 63136.4 — — — 92.2 0.15 17 LYM423 62981.11 — — — 109.3 0.28 38LYM423 62981.12 5.2 0.05 36 — — — LYM403 63620.4 6.8 0.10 78 141.2 0.1679 LYM403 63621.3 5.0 0.08 31 98.3 0.10 24 LYM403 63621.4 6.0 L 57 100.10.04 27 LYM403 63624.3 — — — 95.7 0.12 21 LYM403 63624.4 — — — 114.10.13 44 LYM392 63324.2 6.3 L 66 124.2 0.03 57 LYM392 63328.6 4.6 0.26 2196.1 0.17 22 LYM381 63550.2 4.4 0.22 17 — — — LYM381 63551.2 4.4 0.18 1694.0 0.26 19 LYM336 63068.2 5.6 0.01 48 115.1 0.08 46 LYM336 63071.2 5.90.01 55 104.0 0.03 32 LYM336 63072.1 5.0 0.06 31 — — — CONT. — 3.8 — —79.0 — — LYM475 63096.13 5.6 0.08 57 — — — LYM475 63097.16 6.0 0.04 67111.1 0.27 23 LYM475 63100.1 5.9 0.13 64 — — — LYM472 63114.2 5.3 L 49 —— — LYM472 63114.4 4.2 0.30 17 — — — LYM472 63117.5 5.8 L 63 — — —LYM472 63117.6 5.3 0.04 50 — — — LYM472 63118.8 4.3 0.19 21 — — — LYM43963752.3 5.1 0.09 42 — — — LYM439 63754.3 5.8 0.21 62 121.6 0.22 34LYM427 63772.1 5.3 0.14 48 — — — LYM427 63774.1 4.1 0.22 15 — — — LYM40263778.2 4.6 0.22 29 — — — LYM402 63778.4 4.6 0.03 29 — — — LYM40263780.1 6.4 0.02 79 135.3 0.29 49 LYM400 63121.21 5.8 L 61 116.9 0.22 29LYM400 63121.22 5.0 0.10 41 — — — LYM400 63122.3 6.6 0.01 83 136.6 0.0951 LYM400 63124.13 5.8 0.27 61 — — — LYM386 63391.2 4.8 0.05 34 — — —LYM386 63391.3 5.7 0.05 59 — — — LYM386 63393.3 5.3 0.11 48 — — — LYM38663393.6 4.2 0.09 19 — — — LYM385 63654.1 4.8 0.02 34 — — — LYM38563658.3 5.4 0.02 52 — — — LYM384 63138.13 8.0 0.08 123 145.9 0.13 61LYM384 63139.14 6.7 0.03 87 131.2 0.07 45 LYM384 63139.5 5.3 0.13 50 — —— LYM337 63787.1 6.0 0.07 66 — — — LYM337 63788.2 4.4 0.27 24 — — —LYM337 63788.3 10.2 L 187 184.9 0.02 104 LYM312 63145.1 8.1 0.02 126152.9 0.17 69 LYM312 63198.2 5.4 0.07 50 — — — LYM312 63198.4 4.2 0.2917 — — — LYM312 63201.1 5.2 L 46 — — — LYM312 63201.4 7.3 0.04 105 131.30.09 45 LYM309 63102.3 4.7 0.03 31 — — — LYM309 63103.27 4.7 0.20 31 — —— LYM309 63104.16 5.4 L 52 — — — LYM309 63104.3 6.4 0.02 78 — — — CONT.— 3.6 — — 90.5 — — Table 43. “CONT.”—Control; “Ave.”—Average; “% Incr.”= % increment; “p-val.”—p-value, L—p < 0.01. The transgenes were underthe transcriptional regulation of the new At6669 promoter (SEQ ID NO:4668).

The genes presented in Tables 44 and 45 showed a significant improvementin plant performance since they produced a larger leaf biomass (leafarea) and root biomass (root length and root coverage) (Table 44) and ahigher relative growth rate of leaf area, root coverage and root length(Table 45) when grown under normal growth conditions, compared tocontrol plants. Plants producing larger root biomass have betterpossibilities to absorb larger amount of nitrogen from soil. Plantsproducing larger leaf biomass have better ability to produceassimilates. The genes were cloned under the regulation of aconstitutive promoter (At6669). The evaluation of each gene wasperformed by testing the performance of different number of events. Someof the genes were evaluated in more than one tissue culture assay. Thissecond experiment confirmed the significant increment in leaf and rootperformance. Event with p-value <0.1 was considered statisticallysignificant.

TABLE 44 Genes showing improved plant performance at Normal growthconditions under regulation of At6669 promoter Roots Coverage Leaf Area[cm²] [cm²] Roots Length [cm²] Gene P- % P- % P- % Name Event # Ave.Val. Incr. Ave. Val. Incr. Ave. Val. Incr. LYM490 62920.3 0.5 0.15 23 —— — — — — LYM448 62802.2 0.5 0.02 34 — — — — — — LYM448 62805.2 0.5 0.2225 — — — — — — LYM445 62797.2 0.5 0.12 29 — — — — — — LYM445 62798.3 0.40.30  7 — — — — — — LYM445 62799.2 — — — — — — 7.3 0.04 14 LYM43562872.1 0.5 0.07 41 — — — — — — LYM435 62875.1 0.4 0.04 16 — — — 6.90.28 6 LYM419 62775.1 0.4 0.23 12 — — — — — — LYM419 62775.3 — — — — — —6.9 0.24 8 LYM419 62778.1 0.6 L 65 — — — — — — LYM401 62854.1 0.5 0.0733 — — — — — — LYM401 62856.1 0.5 0.01 29 — — — — — — LYM401 62857.5 0.5L 40 — — — — — — LYM371 62847.1 0.5 0.06 26 — — — — — — LYM371 62847.20.5 0.12 23 — — — — — — LYM371 62851.2 0.5 0.15 41 — — — — — — LYM36762735.5 0.5 0.23 21 — — — — — — LYM367 62737.2 0.4 0.13 13 — — — — — —LYM367 62739.2 0.6 0.10 52 — — — — — — LYM352 62876.2 — — — — — — 7.00.16 9 LYM352 62881.2 0.5 0.21 22 7.7 0.16 30 — — — LYM328 62732.7 0.40.19 13 — — — 6.9 0.23 7 LYM314 62859.1 0.4 0.27 10 — — — — — — LYM31462859.4 — — — — — — 7.3 0.08 13 LYM314 62862.1 0.5 0.07 38 — — — — — —CONT. — 0.4 — — 5.9 — — 6.5 — — LYM504 63308.3 — — — 4.4 0.20  7 5.90.15 6 LYM504 63309.1 0.4 0.22 20 5.4 0.05 34 — — — LYM504 63309.3 0.40.13 27 4.8 0.26 17 6.2 0.03 10 LYM483 63625.2 — — — — — — 6.6 L 17LYM483 63626.6 — — — 5.1 0.04 24 6.6 L 19 LYM483 63629.1 — — — 7.1 0.0175 6.8 0.08 21 LYM483 63629.2 0.4 0.27 31 6.2 0.05 52 6.2 0.09 11 LYM46763563.3 0.4 0.07 24 — — — — — — LYM467 63564.2 — — — 5.3 0.04 29 6.20.04 10 LYM467 63564.3 — — — 6.8 0.01 68 7.1 L 27 LYM467 63565.1 — — —4.7 0.22 16 — — — LYM463 63108.2 0.5 L 59 6.1 L 51 — — — LYM463 63111.12— — — 5.6 0.06 37 6.3 0.01 12 LYM450 63126.2 0.4 0.06 31 6.1 L 49 6.6 L18 LYM450 63127.9 — — — 5.4 0.03 32 6.0 0.06 7 LYM450 63128.1 — — — 4.60.09 13 6.2 L 10 LYM450 63129.2 — — — 5.2 0.15 28 6.3 0.11 12 LYM45063130.4 — — — 5.1 0.10 25 6.2 0.08 11 LYM433 63300.3 — — — 6.5 0.06 607.0 L 25 LYM433 63300.4 — — — 5.4 0.23 32 6.1 0.17 9 LYM433 63301.2 0.4L 38 6.8 L 66 6.8 L 21 LYM433 63301.3 — — — 5.2 0.12 27 — — — LYM43363302.1 0.5 0.04 52 5.8 0.10 44 5.9 0.25 5 LYM425 63132.9 0.4 0.24 24 —— — — — — LYM425 63133.2 — — — 5.4 0.05 32 6.4 0.03 15 LYM425 63134.20.4 0.15 44 6.3 0.03 55 6.7 L 19 LYM425 63135.1 0.4 0.08 24 5.1 L 25 7.1L 26 LYM423 62981.11 — — — 5.2 0.03 27 6.5 0.06 15 LYM423 62981.12 — — —5.1 0.09 26 6.2 0.13 11 LYM423 63056.2 0.4 0.11 21 — — — — — — LYM42363056.3 — — — 6.1 L 50 6.5 L 15 LYM423 63058.2 0.4 0.28 21 5.1 0.04 24 —— — LYM422 63313.3 0.4 0.08 41 5.8 0.02 43 — — — LYM422 63315.3 — — —5.0 L 24 6.1 0.02 8 LYM403 63621.3 0.5 0.03 61 7.0 L 73 6.8 L 22 LYM40363624.4 — — — 5.2 0.10 29 — — — LYM392 63324.2 — — — 8.3 L 103  6.8 0.0221 LYM392 63326.2 0.4 0.29 16 5.3 L 30 6.0 0.09 7 LYM392 63328.4 0.5 L65 6.0 0.03 47 5.9 0.06 6 LYM392 63328.6 0.4 0.05 29 5.6 0.04 37 6.70.06 19 LYM381 63550.2 — — — 5.2 0.11 28 6.6 0.05 19 LYM381 63550.4 0.40.28 22 6.6 L 62 6.9 L 24 LYM381 63551.2 — — — 4.5 0.09 12 6.5 L 16LYM381 63552.1 0.5 L 55 5.1 0.03 25 — — — LYM336 63068.2 0.4 0.04 29 4.50.19 10 — — — LYM336 63071.2 — — — 5.9 0.05 44 — — — LYM336 63073.2 — —— 5.4 L 33 — — — LYM336 63073.4 — — — 7.6 0.03 87 7.1 L 27 CONT. — 0.3 —— 4.1 — — 5.6 — — LYM495 61742.2 0.5 L 51 6.3 0.14 26 — — — LYM49561743.2 0.4 0.18 11 — — — 6.9 0.25 4 LYM495 61744.3 0.5 0.03 40 6.2 0.1024 — — — LYM475 63096.13 0.4 0.23 15 — — — — — — LYM475 63097.4 0.4 0.24 8 — — — — — — LYM475 63098.1 0.5 L 25 6.0 0.15 21 — — — LYM475 63100.10.4 0.04 21 7.0 L 39 7.5 L 13 LYM473 61783.4 0.6 L 72 — — — — — — LYM47361784.2 — — — 5.5 0.30 10 — — — LYM473 61784.3 — — — — — — 7.0 0.21 6LYM473 61786.1 0.4 0.04 14 — — — — — — LYM472 63114.2 0.6 L 67 6.4 0.1227 — — — LYM472 63117.5 0.4 0.02 24 5.8 0.25 16 — — — LYM472 63117.6 0.4L 22 5.9 0.12 18 — — — LYM472 63118.8 0.5 0.01 47 — — — — — — LYM45861812.2 0.5 L 27 — — — — — — LYM458 61814.1 0.7 0.04 94 — — — — — —LYM458 61814.3 — — — — — — 7.1 0.10 7 LYM413 61819.2 0.5 0.03 52 — — — —— — LYM413 61819.3 0.5 0.03 44 — — — — — — LYM413 61822.3 0.4 0.12 22 —— — — — — LYM413 61823.1 0.6 0.02 63 — — — — — — LYM400 63121.22 0.40.08 25 6.4 0.02 28 7.2 L 9 LYM400 63123.6 — — — 5.9 0.20 17 — — —LYM400 63124.13 0.6 0.01 61 — — — — — — LYM380 61824.2 0.6 0.02 59 7.80.05 56 — — — LYM380 61825.2 0.4 0.13 16 — — — — — — LYM380 61825.5 0.50.08 27 7.4 0.04 47 — — — LYM380 61828.3 0.5 0.04 29 — — — — — — LYM38061828.5 — — — 6.0 0.22 21 — — — LYM376 61835.2 0.5 L 41 — — — — — —LYM376 61835.3 0.4 0.12 14 7.1 0.01 43 7.6 0.05 14 LYM376 61836.1 0.40.29 20 — — — — — — LYM376 61839.4 0.5 0.12 46 — — — — — — LYM35461800.2 0.4 0.10 17 5.8 0.16 17 7.0 0.13 6 LYM354 61804.3 0.6 0.03 697.4 L 47 7.5 0.02 13 LYM354 61804.4 0.5 0.08 32 7.5 L 51 7.2 0.04 8LYM344 61788.2 0.5 L 52 — — — — — — LYM344 61788.4 0.6 0.01 55 6.4 L 287.0 0.13 6 LYM344 61790.1 0.5 0.17 26 6.5 0.19 29 — — — LYM344 61790.30.5 0.09 33 — — — — — — LYM330 61840.1 0.6 L 58 7.0 0.02 39 — — — LYM33061841.4 0.4 0.17 14 6.7 0.05 34 7.4 0.05 11 LYM330 61842.4 0.5 0.06 507.2 0.07 44 7.2 0.08 8 LYM330 61844.3 0.7 0.06 82 — — — — — — LYM32761846.1 0.7 0.05 97 7.8 0.15 57 — — — LYM327 61846.3 0.7 0.01 91 6.8 L37 — — — LYM327 61847.1 0.5 0.02 51 6.2 0.18 25 — — — LYM327 61848.2 0.4L 22 — — — — — — LYM327 61849.1 0.5 L 50 7.5 0.02 51 7.4 L 12 LYM30963102.3 0.7 L 85 5.6 0.26 13 — — — LYM309 63103.21 — — — 6.0 0.04 20 7.30.02 9 LYM309 63103.27 0.6 0.04 57 — — — — — — LYM309 63104.16 0.7 L 976.9 L 38 — — — LYM309 63104.3 0.5 L 41 — — — — — — LYM299 61806.4 — — —6.0 0.04 20 7.0 0.10 5 LYM299 61807.3 0.6 0.03 53 — — — — — — LYM29961808.4 0.5 0.01 41 — — — — — — LYM299 61809.2 0.7 L 86 5.6 0.27 12 — —— CONT. — 0.4 — — 5.0 — — 6.6 — — LYM493 61966.4 0.4 0.04 56 5.9 0.11 48— — — LYM493 61968.6 — — — 4.4 0.21 11 — — — LYM493 61969.8 0.4 L 61 6.20.03 56 6.4 0.03 11 LYM480 61960.6 0.4 L 60 4.6 0.29 16 — — — LYM48061961.1 0.4 L 52 4.6 0.22 16 — — — LYM480 61961.12 0.3 0.10 13 — — — — —— LYM480 61962.7 — — — 4.7 0.05 17 6.2 0.15 7 LYM480 61962.8 0.4 L 616.0 0.02 49 6.4 0.06 11 LYM474 61972.5 — — — 4.5 0.19 12 — — — LYM47461975.7 — — — 5.2 0.08 30 — — — LYM474 61977.6 0.3 0.09 14 — — — — — —LYM453 61984.9 0.4 0.28 29 6.0 0.20 51 6.3 0.21 10 LYM453 61985.4 0.40.03 63 6.3 L 58 — — — LYM453 61986.6 0.6 L 101  7.5 L 89 6.2 0.13 7LYM453 61988.6 0.3 L 23 — — — — — — LYM442 61979.5 0.4 0.13 34 6.5 L 626.6 0.01 13 LYM442 61979.6 0.4 L 29 — — — — — — LYM442 61980.5 0.3 0.0613 4.9 0.02 22 — — — LYM442 61983.7 0.5 L 97 7.3 L 82 6.5 0.12 12 LYM44061936.6 0.3 0.10 13 — — — — — — LYM440 61937.6 0.4 L 37 5.3 L 32 — — —LYM440 61937.8 0.4 0.12 28 6.1 L 53 6.2 0.18 7 LYM440 61939.6 0.4 0.0555 5.8 0.08 44 — — — LYM409 61997.2 — — — 5.6 0.06 39 — — — LYM40961998.6 0.4 L 63 7.9 L 98 7.0 L 21 LYM409 61999.3 0.4 0.28 33 — — — — —— LYM396 61900.7 0.4 0.04 32 5.2 0.21 30 — — — LYM396 61901.7 0.5 0.0273 7.2 L 79 6.8 L 18 LYM396 61902.8 0.3 0.05 15 5.3 0.11 33 — — — LYM37262002.1 0.3 0.07 27 — — — — — — LYM372 62003.2 0.3 0.09 12 4.9 0.08 22 —— — LYM372 62006.4 — — — 5.4 L 36 6.1 0.22 6 LYM366 61906.15 0.4 0.13 495.2 0.02 30 — — — LYM366 61906.9 0.3 0.13 22 4.8 0.09 20 — — — LYM33461942.6 — — — 4.4 0.18  9 — — — LYM334 61942.7 0.4 0.02 41 6.2 0.04 566.5 0.16 13 LYM334 61943.12 0.4 0.03 42 4.8 0.04 19 — — — LYM334 61947.70.4 L 35 5.3 0.10 33 — — — LYM319 61920.6 0.3 0.13 22 — — — — — — LYM30161913.4 0.4 0.08 32 4.8 0.12 20 6.3 0.12 9 LYM301 61916.4 — — — — — —6.2 0.17 8 CONT. — 0.3 — — 4.0 — — 5.8 — — LYM504 63308.3 0.4 L 35 6.20.01 27 — — — LYM504 63309.3 0.4 0.18 12 5.9 0.02 20 7.1 L 21 LYM48363625.2 0.4 0.02 26 6.6 0.10 34 6.7 L 13 LYM483 63626.6 0.4 0.04 15 6.10.07 23 6.9 0.03 17 LYM483 63626.7 0.4 0.14 22 — — — 6.4 0.13 9 LYM48363629.1 — — — — — — 6.5 0.12 11 LYM467 63563.3 — — — 6.1 0.03 23 6.30.28 6 LYM467 63564.2 0.5 0.01 37 5.7 0.26 16 — — — LYM467 63564.3 0.5 L54 8.1 L 64 7.1 L 20 LYM467 63565.4 0.5 0.02 40 — — — — — — LYM46363108.2 0.4 0.03 33 — — — — — — LYM463 63110.9 0.4 0.20 21 — — — — — —LYM463 63111.12 0.4 L 27 7.0 0.05 41 6.9 0.02 17 LYM463 63113.2 0.5 0.0358 — — — — — — LYM450 63127.9 0.4 L 33 5.8 0.03 17 6.1 0.26 4 LYM45063128.1 0.4 0.06 15 7.1 0.06 44 6.7 L 13 LYM433 63300.3 0.4 0.03 21 6.70.01 35 6.8 0.10 16 LYM433 63300.4 0.5 L 41 8.3 L 68 7.1 L 21 LYM43363301.3 0.4 0.02 23 7.2 L 46 6.8 L 15 LYM425 63132.9 0.4 0.12 31 5.80.29 18 — — — LYM425 63133.2 0.4 0.01 19 6.6 0.03 33 6.3 0.15 7 LYM42563134.2 — — — — — — 6.4 0.21 9 LYM425 63135.1 — — — — — — 6.6 0.12 13LYM423 62981.11 — — — — — — 6.5 0.26 10 LYM423 63056.3 0.4 0.20  9 — — —6.2 0.12 5 LYM422 63314.3 0.4 0.18 14 6.1 L 24 6.6 L 12 LYM422 63315.40.4 0.14 11 6.3 0.03 28 6.9 0.03 18 LYM403 63620.4 0.5 0.07 36 6.1 0.2124 6.2 0.30 5 LYM403 63621.3 0.5 L 37 — — — — — — LYM403 63621.4 0.40.06 25 7.1 0.03 43 — — — LYM403 63624.3 0.4 L 29 — — — 6.4 0.02 8LYM403 63624.4 0.4 0.07 20 6.1 0.04 24 — — — LYM392 63324.2 0.5 L 48 — —— — — — LYM392 63326.2 0.4 0.08 16 5.5 0.29 12 6.4 0.03 9 LYM392 63328.3— — — 5.7 0.14 15 6.4 0.10 9 LYM392 63328.6 0.4 0.10 20 — — — — — —LYM381 63551.2 0.5 L 45 5.9 0.27 20 6.4 0.28 8 LYM336 63068.2 0.4 L 33 —— — — — — LYM336 63071.2 0.5 L 43 6.1 L 23 6.5 0.01 10 LYM336 63073.20.4 0.18 23 — — — — — — CONT. — 0.3 — — 4.9 — — 5.9 — — LYM475 63096.130.5 0.12 17 7.6 0.20 18 — — — LYM475 63097.16 0.6 L 24 8.0 L 23 — — —LYM475 63098.1 0.5 0.25 12 — — — — — — LYM475 63100.1 0.6 0.12 26 — — —— — — LYM472 63114.2 0.6 0.02 23 7.2 0.08 12 — — — LYM472 63117.5 0.7 L52 — — — — — — LYM472 63117.6 0.6 0.07 32 7.5 0.10 16 7.2 0.08 4 LYM43963752.3 0.5 0.20 13 — — — — — — LYM439 63752.5 — — — — — — 7.4 0.08 7LYM439 63754.3 0.6 0.03 24 7.9 L 22 7.3 0.13 5 LYM427 63772.1 0.5 0.1715 7.6 0.23 18 — — — LYM402 63778.2 0.5 0.26 14 6.9 0.24  6 — — — LYM40263778.4 0.5 0.07 21 7.3 0.08 14 7.5 0.08 9 LYM402 63780.1 0.7 0.19 47 —— — — — — LYM400 63121.21 0.6 L 40 — — — — — — LYM400 63121.22 0.6 0.1224 — — — — — — LYM400 63122.3 0.6 0.01 36 9.2 0.03 43 7.3 0.25 5 LYM40063124.13 0.6 0.15 39 — — — — — — LYM386 63391.2 0.6 0.04 28 — — — — — —LYM386 63391.3 0.6 0.07 42 — — — — — — LYM386 63393.3 0.5 0.28 17 8.60.05 33 7.5 0.02 9 LYM385 63654.1 0.5 0.05 21 7.5 0.02 16 — — — LYM38563658.1 — — — 7.8 0.01 21 — — — LYM385 63658.3 0.6 0.03 42 7.8 0.02 227.4 0.10 7 LYM384 63138.13 0.7 0.06 63 8.0 0.11 24 — — — LYM384 63139.140.7 0.02 47 8.7 0.02 35 7.7 0.08 12 LYM384 63139.5 0.6 0.17 25 7.6 0.2118 — — — LYM337 63787.1 0.6 0.07 23 — — — — — — LYM337 63787.5 — — — 7.90.05 22 7.6 L 9 LYM337 63788.3 0.8 L 82 8.9 0.02 39 — — — LYM312 63145.10.8 0.01 73 — — — — — — LYM312 63198.2 0.6 0.02 38 — — — — — — LYM31263201.1 0.6 0.04 37 — — — — — — LYM312 63201.4 0.7 0.06 59 7.9 0.26 22 —— — LYM309 63102.3 0.6 L 24 — — — — — — LYM309 63103.27 0.6 L 35 7.00.19  8 — — — LYM309 63104.16 0.6 0.02 24 — — — — — — LYM309 63104.3 0.7L 47 7.3 0.13 13 — — — CONT. — 0.5 — — 6.4 — — 6.9 — — Table 44.“CONT.”—Control; “Ave.”—Average; “% Incr.” = % increment;“p-val.”—p-value, L - p < 0.01.

TABLE 45 Genes showing improved plant performance at Normal growthconditions under regulation of At6669 promoter RGR Of Root RGR Of RootsRGR Of Leaf Area Coverage Length Gene P- % P- % P- % Name Event # Ave.Val. Incr. Ave. Val. Incr. Ave. Val. Incr. LYM490 62920.3 0.0 0.15 21 —— — — — — LYM448 62802.2 0.1 0.01 33 — — — — — — LYM445 62797.2 0.0 0.0926 — — — — — — LYM435 62872.1 0.1 0.01 38 — — — — — — LYM435 62875.1 0.00.28 12 — — — — — — LYM419 62775.3 — — — — — — 0.7 0.30 12 LYM41962778.1 0.1 L 77 — — — — — — LYM401 62854.1 0.1 0.03 31 — — — — — —LYM401 62856.1 0.0 0.04 25 — — — — — — LYM401 62857.5 0.1 L 43 — — — — —— LYM371 62847.1 0.0 0.05 25 — — — — — — LYM371 62847.2 0.0 0.26 15 — —— — — — LYM371 62851.2 0.1 0.03 43 — — — — — — LYM367 62735.5 0.0 0.2118 — — — — — — LYM367 62737.2 0.0 0.08 20 — — — — — — LYM367 62739.2 0.10.02 50 — — — — — — LYM352 62881.2 0.0 0.24 17 — — — — — — LYM31462859.4 — — — — — — 0.7 0.11 19 LYM314 62862.1 0.1 0.03 35 — — — — — —CONT. — 0.0 — — — — — 0.6 — — LYM504 63309.1 0.0 0.26 23 — — — — — —LYM504 63309.3 — — — — — — 0.6 L 20 LYM483 63625.2 — — — — — — 0.6 L 22LYM483 63626.6 — — — — — — 0.5 L 19 LYM483 63629.1 0.0 0.18 29 — — — 0.6L 28 LYM483 63629.2 0.0 0.11 39 — — — 0.6 L 23 LYM467 63564.2 — — — — —— 0.5 0.12 11 LYM467 63564.3 — — — — — — 0.6 L 23 LYM467 63565.4 — — — —— — 0.5 0.21 10 LYM463 63108.2 0.1 L 72 — — — 0.5 0.28 8 LYM463 63111.12— — — — — — 0.5 0.04 14 LYM450 63126.2 0.0 0.03 43 — — — 0.6 L 28 LYM45063128.1 — — — — — — 0.5 0.01 16 LYM450 63130.4 — — — — — — 0.5 0.22 9LYM433 63300.3 — — — — — — 0.6 L 36 LYM433 63300.4 0.0 0.28 25 — — — 0.50.24 10 LYM433 63301.2 0.0 0.02 45 — — — 0.6 L 21 LYM433 63302.1 0.00.01 58 — — — 0.6 L 23 LYM425 63132.9 0.0 0.15 30 — — — — — — LYM42563133.2 — — — — — — 0.5 0.02 17 LYM425 63134.2 0.0 0.08 45 — — — 0.50.01 17 LYM425 63135.1 0.0 0.15 27 — — — 0.6 L 27 LYM423 62981.11 — — —— — — 0.6 L 26 LYM423 62981.12 — — — — — — 0.5 0.07 15 LYM423 63056.20.0 0.27 20 — — — — — — LYM423 63056.3 — — — — — — 0.5 0.03 14 LYM42363058.2 0.0 0.22 25 — — — 0.5 0.09 11 LYM422 63313.3 0.0 0.09 37 — — —0.5 0.12 17 LYM422 63315.3 — — — — — — 0.5 0.09 10 LYM403 63621.3 0.00.01 62 — — — 0.6 L 28 LYM403 63624.3 — — — — — — 0.5 0.02 16 LYM40363624.4 — — — — — — 0.5 0.05 17 LYM392 63324.2 — — — — — — 0.6 L 29LYM392 63326.2 — — — — — — 0.5 0.12 11 LYM392 63328.4 0.1 L 79 — — — 0.6L 24 LYM392 63328.6 0.0 0.08 34 — — — 0.6 L 28 LYM381 63550.2 — — — — —— 0.6 L 25 LYM381 63550.4 — — — — — — 0.6 L 25 LYM381 63551.2 — — — — —— 0.5 L 19 LYM381 63552.1 0.0 L 54 — — — 0.5 0.23 8 LYM336 63068.2 0.00.09 31 — — — 0.5 0.21 12 LYM336 63073.2 0.0 0.21 33 — — — — — — LYM33663073.4 — — — — — — 0.6 L 33 CONT. — 0.0 — — — — — 0.5 — — LYM49561742.2 0.1 L 52 — — — — — — LYM495 61744.1 0.0 0.22 15 — — — — — —LYM495 61744.3 0.1 L 39 — — — — — — LYM475 63096.13 0.0 0.15 16 — — — —— — LYM475 63098.1 0.0 0.01 24 — — — — — — LYM475 63100.1 0.0 0.05 20 —— — 0.7 0.02 16 LYM473 61783.4 0.1 L 67 — — — — — — LYM473 61786.1 0.00.06 17 — — — — — — LYM472 63114.2 0.1 L 63 — — — — — — LYM472 63117.50.0 0.03 22 — — — — — — LYM472 63117.6 0.0 0.21 12 — — — — — — LYM47263118.8 0.1 L 47 — — — — — — LYM458 61812.2 0.0 0.02 22 — — — — — —LYM458 61814.1 0.1 L 90 — — — — — — LYM413 61819.2 0.1 L 43 — — — — — —LYM413 61819.3 0.0 0.02 30 — — — — — — LYM413 61822.3 0.0 0.03 25 — — —— — — LYM413 61823.1 0.1 L 68 — — — — — — LYM400 63121.22 0.0 0.02 26 —— — 0.6 0.25 8 LYM400 63124.13 0.1 L 59 — — — — — — LYM380 61824.2 0.1 L61 — — — — — — LYM380 61825.2 0.0 0.23 12 — — — — — — LYM380 61825.5 0.00.04 24 — — — — — — LYM380 61828.3 0.0 0.02 26 — — — — — — LYM37661835.2 0.0 L 33 — — — — — — LYM376 61835.3 0.0 0.14 15 — — — 0.6 0.1213 LYM376 61836.1 0.0 0.19 20 — — — — — — LYM376 61839.4 0.1 0.04 40 — —— — — — LYM354 61800.2 0.0 0.05 20 — — — 0.6 0.06 13 LYM354 61804.3 0.1L 58 — — — — — — LYM354 61804.4 0.0 0.02 29 — — — 0.6 0.26 8 LYM34461788.2 0.1 L 47 — — — — — — LYM344 61788.4 0.1 L 46 — — — — — — LYM34461790.1 0.0 0.04 28 — — — — — — LYM344 61790.3 0.0 L 37 — — — — — —LYM330 61840.1 0.1 L 56 — — — — — — LYM330 61841.4 0.0 0.13 15 — — — — —— LYM330 61842.4 0.1 L 47 — — — — — — LYM330 61844.3 0.1 L 77 — — — — —— LYM327 61846.1 0.1 L 85 — — — — — — LYM327 61846.3 0.1 L 88 — — — — —— LYM327 61847.1 0.1 L 51 — — — — — — LYM327 61848.2 0.0 0.03 20 — — — —— — LYM327 61849.1 0.1 L 43 — — — 0.7 0.03 15 LYM309 63102.3 0.1 L 82 —— — — — — LYM309 63103.21 — — — — — — 0.6 0.22 8 LYM309 63103.27 0.1 L50 — — — — — — LYM309 63104.16 0.1 L 91 — — — — — — LYM309 63104.3 0.1 L44 — — — — — — LYM299 61807.3 0.1 L 58 — — — — — — LYM299 61808.4 0.1 L40 — — — — — — LYM299 61809.2 0.1 L 91 — — — — — — CONT. — 0.0 — — — — —0.6 — — LYM493 61966.4 0.0 L 64 — — — — — — LYM493 61969.8 0.0 L 69 — —— 0.6 0.12 16 LYM480 61960.6 0.0 L 65 — — — — — — LYM480 61961.1 0.0 L62 — — — — — — LYM480 61961.12 0.0 0.13 19 — — — — — — LYM480 61962.80.0 L 66 — — — — — — LYM474 61977.6 0.0 0.17 18 — — — — — — LYM45361984.7 0.0 0.18 18 — — — — — — LYM453 61984.9 0.0 0.08 30 — — — 0.60.10 27 LYM453 61985.4 0.0 L 64 — — — — — — LYM453 61986.6 0.1 L 113  —— — 0.6 0.04 20 LYM453 61988.6 0.0 0.06 24 — — — — — — LYM442 61979.50.0 0.05 34 — — — 0.6 0.03 22 LYM442 61979.6 0.0 L 39 — — — — — — LYM44261980.5 0.0 0.15 18 — — — — — — LYM442 61983.7 0.1 L 110  — — — 0.6 0.1417 LYM440 61937.6 0.0 L 40 — — — — — — LYM440 61937.8 0.0 0.15 26 — — —— — — LYM440 61939.6 0.0 L 57 — — — — — — LYM409 61997.1 0.0 0.15 28 — —— — — — LYM409 61998.6 0.0 L 61 — — — 0.6 0.05 20 LYM409 61999.3 0.00.10 31 — — — — — — LYM396 61900.7 0.0 L 41 — — — — — — LYM396 61901.70.0 L 86 — — — 0.6 0.01 25 LYM396 61902.8 0.0 0.09 21 — — — — — — LYM37262002.1 0.0 0.02 37 — — — — — — LYM372 62003.2 0.0 0.19 16 — — — — — —LYM372 62006.4 — — — — — — 0.6 0.20 13 LYM366 61906.15 0.0 0.02 55 — — —— — — LYM366 61906.9 0.0 0.10 24 — — — — — — LYM334 61942.6 0.0 0.26 19— — — 0.6 0.29 12 LYM334 61942.7 0.0 L 42 — — — 0.6 0.16 17 LYM33461943.12 0.0 L 42 — — — — — — LYM334 61947.7 0.0 L 36 — — — — — — LYM31961920.6 0.0 0.07 25 — — — — — — LYM301 61913.4 0.0 0.05 31 — — — — — —CONT. — 0.0 — — — — — 0.5 — — LYM504 63307.1 0.0 0.28 13 — — — — — —LYM504 63308.3 0.0 L 45 — — — — — — LYM504 63309.1 — — — — — — 0.6 0.2112 LYM504 63309.3 — — — — — — 0.7 L 28 LYM483 63625.2 0.0 L 32 — — — 0.60.12 11 LYM483 63626.6 0.0 0.12 17 — — — 0.6 0.01 22 LYM483 63626.7 0.00.07 26 — — — 0.6 0.07 13 LYM483 63629.1 — — — — — — 0.6 0.04 16 LYM46763563.3 — — — — — — 0.6 0.28 8 LYM467 63564.2 0.0 L 35 — — — — — —LYM467 63564.3 0.1 L 54 — — — 0.6 0.06 14 LYM467 63565.1 0.0 0.29 16 — —— — — — LYM467 63565.4 0.0 L 44 — — — — — — LYM463 63108.2 0.0 L 46 — —— — — — LYM463 63110.9 0.0 0.16 21 — — — — — — LYM463 63111.12 0.0 L 37— — — 0.6 L 23 LYM463 63113.2 0.1 L 58 — — — — — — LYM450 63126.2 0.00.17 19 — — — — — — LYM450 63127.9 0.0 L 42 — — — — — — LYM450 63128.10.0 0.02 28 — — — 0.6 0.11 11 LYM433 63300.3 0.0 0.05 23 — — — 0.6 0.1213 LYM433 63300.4 0.0 L 49 — — — 0.6 L 22 LYM433 63301.3 0.0 L 34 — — —0.6 L 21 LYM433 63302.1 — — — — — — 0.6 0.09 14 LYM425 63132.9 0.0 0.0237 — — — 0.6 0.13 14 LYM425 63133.2 0.0 0.04 24 — — — — — — LYM42563134.2 0.0 0.17 18 — — — 0.6 0.04 18 LYM425 63135.1 — — — — — — 0.60.06 17 LYM423 62981.11 — — — — — — 0.6 0.20 11 LYM422 63314.3 0.0 0.0922 — — — 0.6 0.04 16 LYM422 63315.4 0.0 0.21 14 — — — 0.6 0.10 15 LYM40363620.4 0.0 L 41 — — — — — — LYM403 63621.3 0.0 L 39 — — — — — — LYM40363621.4 0.0 0.03 30 — — — — — — LYM403 63624.3 0.0 0.02 28 — — — 0.60.16 9 LYM403 63624.4 0.0 0.08 22 — — — 0.6 0.18 11 LYM392 63324.2 0.1 L57 — — — — — — LYM392 63326.2 0.0 0.07 21 — — — 0.6 0.29 7 LYM39263328.3 — — — — — — 0.6 0.04 14 LYM392 63328.4 0.0 0.29 12 — — — — — —LYM392 63328.6 0.0 0.10 21 — — — — — — LYM381 63550.3 — — — — — — 0.60.07 14 LYM381 63551.2 0.0 L 44 — — — — — — LYM336 63068.2 0.0 L 38 — —— — — — LYM336 63071.2 0.0 L 49 — — — 0.6 0.17 9 LYM336 63073.2 0.0 0.0628 — — — — — — CONT. — 0.0 — — — — — 0.5 — — LYM475 63096.13 0.0 0.21 14— — — — — — LYM475 63097.16 0.1 0.02 24 — — — — — — LYM475 63100.1 0.10.28 14 — — — — — — LYM472 63114.2 0.1 0.03 23 — — — — — — LYM47263117.5 0.1 L 36 — — — — — — LYM472 63117.6 0.1 0.03 28 — — — — — —LYM439 63752.3 0.1 0.10 18 — — — — — — LYM439 63753.1 — — — — — — 0.60.20 8 LYM439 63754.3 0.1 0.03 24 — — — 0.6 0.10 11 LYM402 63778.2 0.10.23 14 — — — — — — LYM402 63778.4 0.1 0.17 16 — — — 0.6 0.13 10 LYM40263780.1 0.1 0.03 39 — — — — — — LYM400 63121.21 0.1 0.01 27 — — — — — —LYM400 63121.22 0.1 0.17 19 — — — — — — LYM400 63122.3 0.1 L 41 — — —0.6 0.16 11 LYM400 63124.13 0.1 0.05 39 — — — — — — LYM386 63391.2 0.10.04 24 — — — — — — LYM386 63391.3 0.1 0.02 37 — — — — — — LYM38663393.3 — — — — — — 0.6 0.08 11 LYM385 63654.1 0.1 0.03 23 — — — — — —LYM385 63658.3 0.1 0.01 35 — — — — — — LYM384 63138.13 0.1 L 60 — — — —— — LYM384 63139.14 0.1 L 43 — — — 0.6 0.14 11 LYM384 63139.5 0.1 0.1522 — — — 0.6 0.29 6 LYM337 63787.1 0.1 0.04 24 — — — 0.6 0.21 10 LYM33763787.5 — — — — — — 0.7 0.01 14 LYM337 63788.2 — — — — — — 0.7 0.04 12LYM337 63788.3 0.1 L 81 — — — — — — LYM312 63145.1 0.1 L 70 — — — — — —LYM312 63198.2 0.1 L 32 — — — — — — LYM312 63201.1 0.1 0.04 26 — — — — —— LYM312 63201.4 0.1 L 54 — — — — — — LYM309 63102.3 0.0 0.15 13 — — — —— — LYM309 63103.27 0.1 0.02 26 — — — — — — LYM309 63104.16 0.1 0.06 20— — — — — — LYM309 63104.3 0.1 L 46 — — — — — — CONT. — 0.0 — — — — —0.6 — — Table 45. “CONT.”—Control; “Ave.”—Average; “% Incr.” = %increment; “p-val.”—p-value, L - p < 0.01. The transgenes were under thetranscriptional regulation of the new At6669 promoter (SEQ ID NO: 4668).

Results from T1 Plants

The genes presented in Tables 46-48 showed a significant improvement inplant biomass and root development since they produced a higher biomass(dry and fresh weight, Table 46), a larger leaf and root biomass (leafarea, root length and root coverage) (Table 47), and a higher relativegrowth rate of leaf area, root coverage and root length (Table 48) whengrown under normal growth conditions, compared to control plants. Plantsproducing larger root biomass have better possibilities to absorb largeramount of nitrogen from soil. Plants producing larger leaf biomass hasbetter ability to produce assimilates). The genes were cloned under theregulation of a constitutive promoter (At6669; SEQ ID NO:4668). Theevaluation of each gene was performed by testing the performance ofdifferent number of events. Some of the genes were evaluated in morethan one tissue culture assay. This second experiment confirmed thesignificant increment in leaf and root performance. Event with p-value<0.1 was considered statistically significant.

Tables 46-48 summarize the observed phenotypes of transgenic plantsexpressing the gene constructs using the TC-T1 Assays.

TABLE 46 Genes showing improved plant performance at Normal growthconditions under regulation of A6669 promoter Dry Weight [mg] FreshWeight [mg] Gene Name Ave. P-Val. % Incr. Ave. P-Val. % Incr. LYM452 7.10.26 22 — — — LYM449 7.1 0.03 22 140.3 0.04 22 LYM379 6.6 0.30 14 — — —CONT. 5.8 — — 115.3 — — LYM433 8.0 0.13 21 — — — LYM427 8.3 0.07 25 — —— LYM368_H4 8.4 0.13 27 190.5 0.25 51 LYM337 9.1 0.13 36 151.3 0.21 20LYM297 8.4 0.13 27 — — — CONT. 6.6 — — 125.9 — — Table 46.“CONT.”—Control; “Ave.”—Average; “% Incr.” = % increment;“p-val.”—p-value, L - p < 0.01. The transgenes were under thetranscriptional regulation of the new At6669 promoter (SEQ ID NO: 4668).

TABLE 47 Genes showing improved plant performance at Normal growthconditions under regulation of At6669 promoter Leaf Area [cm²] RootsCoverage [cm²] Roots Length [cm²] P- % P- % P- % Gene Name Ave. Val.Incr. Ave. Val. Incr. Ave. Val. Incr. LYM497 0.5 0.10 9 — — — — — —LYM485 — — — — — — 3.6 0.15 12 LYM452 — — — 3.0 0.09 33 3.6 0.05 12LYM434 — — — — — — 3.7 0.11 14 LYM420 — — — 2.5 0.22 14 3.6 0.12 12LYM397_H2 0.5 0.18 16 3.3 0.11 49 3.8 0.05 19 LYM360 — — — — — — 3.50.29 10 LYM357 — — — — — — 3.5 0.23 9 LYM350 — — — 2.5 0.23 13 3.4 0.227 CONT. 0.5 — — 2.2 — — 3.2 — — LYM504 — — — — — — 4.1 0.18 9 LYM484 — —— — — — 4.3 0.02 17 LYM483 — — — 3.5 0.24 30 4.7 0.07 26 LYM468 — — — —— — 4.0 0.30 7 LYM46 — — — 3.6 0.03 35 4.4 L 19 LYM439 — — — 4.3 0.06 615.0 0.01 33 LYM433 — — — 5.1 L 91 5.2 L 40 LYM428 — — — 3.9 0.18 47 4.90.05 33 LYM427 — — — 3.5 0.19 30 4.3 0.07 14 LYM422 — — — 3.6 0.03 355.0 L 34 LYM417 — — — 4.5 0.05 67 5.0 L 33 LYM403 — — — 3.5 0.16 31 4.7L 26 LYM402 — — — 4.4 L 63 5.2 0.01 40 LYM398 — — — 3.5 0.05 31 4.8 L 29LYM392 — — — 3.5 0.14 29 4.1 0.25 11 LYM391 — — — 3.8 0.01 44 4.7 0.0325 LYM386 — — — 3.9 0.02 46 4.8 L 29 LYM385 — — — 4.1 L 55 5.1 0.02 37LYM381 — — — — — — 5.0 L 33 LYM349 — — — 4.7 0.04 75 5.4 L 44 LYM337 — —— 3.9 0.04 46 4.6 0.01 23 LYM336 — — — — — — 4.1 0.12 11 LYM333 — — —4.8 L 78 5.0 L 35 LYM308 — — — 3.7 0.05 39 4.6 L 24 LYM307_H7 — — — 3.20.26 19 4.0 0.29 7 LYM304_H3 — — — 3.6 0.07 36 4.7 0.03 27 LYM298 — — —3.6 0.11 36 4.9 L 30 LYM297 — — — 4.4 L 64 5.0 0.01 35 CONT. — — — 2.7 —— 3.7 — — LYM421 — — — — — — 3.9 0.35 7.5 CONT. — — — — — — 3.5 — —Table 47. “CONT.”—Control; “Ave.”—Average; “% Incr.” = % increment;“p-val.”—p-value, L - p < 0.01. The transgenes were under thetranscriptional regulation of the new At6669 promoter (SEQ ID NO: 4668).

TABLE 48 Genes showing improved plant performance at Normal growthconditions under regulation of At6669 promoter RGR Of RGR Of Leaf AreaRoot Coverage RGR Of Roots Length Gene % % % Name Ave. P-Val. Incr. Ave.P-Val. Incr. Ave. P-Val. Incr. LYM485 — — — — — — 0.4 0.06 16 LYM478 — —— — — — 0.4 0.28 9 LYM452 — — — — — — 0.4 0.03 17 LYM434 — — — — — — 0.40.02 20 LYM420 — — — — — — 0.4 0.10 13 LYM397_H2 0.1 0.09 22 — — — 0.5 L29 LYM360 — — — — — — 0.4 0.19 14 LYM357 — — — — — — 0.4 0.08 14 LYM350— — — — — — 0.4 0.09 13 LYM326_H4 — — — — — — 0.4 0.13 13 LYM315 — — — —— — 0.4 0.21 11 CONT. 0.1 — — — — — 0.4 — — LYM484 — — — — — — 0.5 0.0717 LYM483 — — — — — — 0.5 0.01 28 LYM46 — — — — — — 0.5 0.01 23 LYM439 —— — — — — 0.6 L 36 LYM433 — — — — — — 0.6 L 43 LYM428 — — — — — — 0.6 L36 LYM427 — — — — — — 0.5 0.12 14 LYM422 — — — — — — 0.6 L 36 LYM417 — —— — — — 0.6 L 35 LYM403 — — — — — — 0.5 L 28 LYM402 — — — — — — 0.6 L 44LYM398 — — — — — — 0.5 L 30 LYM392 — — — — — — 0.5 0.28 11 LYM391 — — —— — — 0.5 L 27 LYM386 — — — — — — 0.5 L 31 LYM385 — — — — — — 0.6 L 39LYM381 — — — — — — 0.6 L 36 LYM349 — — — — — — 0.6 L 48 LYM337 0.1 0.1533 — — — 0.5 0.02 22 LYM336 — — — — — — 0.5 0.22 13 LYM333 — — — — — —0.6 L 37 LYM312 — — — — — — 0.5 0.21 13 LYM308 — — — — — — 0.5 L 26LYM304_H3 — — — — — — 0.5 L 28 LYM298 — — — — — — 0.6 L 35 LYM297 — — —— — — 0.6 L 38 CONT. 0.1 — — — — — 0.4 — — Table 48. “CONT.”—Control;“Ave.”—Average; “% Incr.” = % increment; “p-val.”—p-value, L - p < 0.01.The transgenes were under the transcriptional regulation of the newAt6669 promoter (SEQ ID NO: 4668).

These results demonstrate that the polynucleotides of the invention arecapable of improving yield and additional valuable importantagricultural traits such as increase of biomass, abiotic stresstolerance, nitrogen use efficiency, yield, vigor, fiber yield and/orquality. Thus, transformed plants showing improved fresh and dry weightdemonstrate the gene capacity to improve biomass a key trait of cropsfor forage and plant productivity; transformed plants showingimprovement of seed yield demonstrate the genes capacity to improveplant productivity; transformed plants showing improvement of plotcoverage and rosette diameter demonstrate the genes capacity to improveplant drought resistance as they reduce the loss of soil water by simpleevaporation and reduce the competition with weeds; hence reduce the needto use herbicides to control weeds. Transformed plants showingimprovement of relative growth rate of various organs (leaf and root)demonstrate the gene capacity to promote plant growth and henceshortening the needed growth period and/or alternatively improving theutilization of available nutrients and water leading to increase of landproductivity; Transformed plants showing improvement of organ number asdemonstrated by the leaf number parameter exhibit a potential to improvebiomass yield important for forage crops and improve the plantproductivity; Transformed plants showing increased root length andcoverage demonstrate the gene capacity to improve drought resistance andbetter utilization of fertilizers as the roots can reach larger soilvolume; Transformed plants showing improvement of leaf petiole relativearea and leaf blade area demonstrate the genes capacity to cope withlimited light intensities results from increasing the plant populationdensities and hence improve land productivity.

Although the invention has been described in conjunction with specificembodiments thereof, it is evident that many alternatives, modificationsand variations will be apparent to those skilled in the art.Accordingly, it is intended to embrace all such alternatives,modifications and variations that fall within the spirit and broad scopeof the appended claims.

All publications, patents and patent applications mentioned in thisspecification are herein incorporated in their entirety by referenceinto the specification, to the same extent as if each individualpublication, patent or patent application was specifically andindividually indicated to be incorporated herein by reference. Inaddition, citation or identification of any reference in thisapplication shall not be construed as an admission that such referenceis available as prior art to the present invention. To the extent thatsection headings are used, they should not be construed as necessarilylimiting.

What is claimed is:
 1. A method of increasing yield, biomass, growthrate, vigor, oil content, fiber yield, fiber quality, abiotic stresstolerance, and/or nitrogen use efficiency of a plant, comprisingexpressing within the plant an exogenous polynucleotide comprising anucleic acid sequence encoding a polypeptide at least 80% identical toSEQ ID NO: 517, 481-516, 518-792, 2947-4662 or 4663, thereby increasingthe yield, biomass, growth rate, vigor, oil content, fiber yield, fiberquality, abiotic stress tolerance, and/or nitrogen use efficiency of theplant.
 2. The method of claim 1, wherein said polypeptide is selectedfrom the group consisting of SEQ ID NOs: 517, 481-516, 518-792, and2947-4663.
 3. The method of claim 1, wherein said nucleic acid sequenceencoding said polypeptide is at least 80% identical to SEQ ID NO: 321,1-320, 322-480, 793-2945 or
 2946. 4. The method of claim 1, wherein saidnucleic acid sequence is selected from the group consisting of SEQ IDNOs: 321, 1-320, 322-480, and 793-2946.
 5. A nucleic acid constructcomprising an isolated polynucleotide comprising a nucleic acid sequenceencoding a polypeptide which comprises an amino acid sequence at least80% identical to the amino acid sequence set forth in SEQ ID NO: 517,481-516, 518-792, 2947-4662 or 4663, and a heterologous promoter fordirecting transcription of said isolated polynucleotide in a host cell.6. The nucleic acid construct of claim 5, wherein said promoter is aplant promoter.
 7. The nucleic acid construct of claim 5, wherein saidpromoter is heterologous to said isolated polynucleotide and/or to saidhost cell.
 8. A plant cell exogenously expressing the nucleic acidconstruct of claim
 5. 9. A plant exogenously expressing the nucleic acidconstruct of claim
 5. 10. The method of claim 1, wherein saidpolypeptide is at least 85% identical to the amino acid sequenceselected from the group consisting of SEQ ID NOs: 517, 481-516, 518-792,and 2947-4663.
 11. The method of claim 1, wherein said polypeptide is atleast 90% identical to the amino acid sequence selected from the groupconsisting of SEQ ID NOs: 517, 481-516, 518-792, and 2947-4663.
 12. Themethod of claim 1, wherein said polypeptide is at least 95% identical tothe amino acid sequence selected from the group consisting of SEQ IDNOs: 517, 481-516, 518-792, and 2947-4663.
 13. The method of claim 1,wherein said polypeptide is at least 98% identical to the amino acidsequence selected from the group consisting of SEQ ID NOs: 517, 481-516,518-792, and 2947-4663.
 14. The method of claim 1, further comprisinggrowing the plant expressing said exogenous polynucleotide under theabiotic stress.
 15. The method of claim 1, wherein said abiotic stressis selected from the group consisting of salinity, drought, waterdeprivation, flood, etiolation, low temperature, high temperature, heavymetal toxicity, anaerobiosis, nutrient deficiency, nutrient excess,atmospheric pollution and UV irradiation.
 16. The method of claim 1,wherein the yield comprises seed yield or oil yield.
 17. The method ofclaim 1, further comprising growing the plant expressing said exogenouspolynucleotide under nitrogen-limiting conditions.
 18. A method ofproducing seeds of a crop, comprising: (a) selecting a parent planttransformed with an exogenous polynucleotide comprising a nucleic acidsequence encoding a polypeptide at least 80% identical to SEQ ID NO:517, 481-516, 518-792, 2947-4662 or 4663, wherein said parent plantexhibits an increased trait selected from the group consisting of:increased yield, increased biomass, increased growth rate, increasedvigor, increased oil content, increased fiber yield, increased fiberquality, increased abiotic stress tolerance, and/or increased nitrogenuse efficiency as compared to a non-transformed plant which is grownunder the same growth conditions, and; (b) growing a seed producingplant from said parent plant resultant of step (a), wherein said seedproducing plant which comprises said exogenous polynucleotide has saidincreased trait, and; (c) producing seeds from said seed producing plantresultant of step (b), thereby producing seeds of the crop.
 19. Themethod of claim 18, wherein said polypeptide is at least 95% identicalto the amino acid sequence selected from the group consisting of SEQ IDNOs: 517, 481-516, 518-792, and 2947-4663.
 20. The method of claim 18,wherein said polypeptide is selected from the group consisting of SEQ IDNOs: 517, 481-516, 518-792, and 2947-4663.